LOCUS       sequence-44236-e         669 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, 
            Eichtinger M, Jiang T, Foley JE, Winfrey RJ, Townsend JA, 
            Unger-Wallace E, Sander JD, Muller-Lerch F, Fu F, Pearlberg J, 
            Goebel C, Dassie JP, Pruett-Miller SM, Porteus MH, Sgroi DC, Iafrate
            AJ, Dobbs D, McCray PB, Cathomen T, Voytas DF, Joung JK
  TITLE     Rapid ""open-source"" engineering of customized zinc-finger 
            nucleases for highly efficient gene modification.
  JOURNAL   Mol Cell. 2008 Jul 25. 31(2):294-301.
  PUBMED    18657511
REFERENCE   2  (bases 1 to 669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2008 Jul 25. 31(2):294-301."
COMMENT     Sequence Label: flt1_R (OZ586)
FEATURES             Location/Qualifiers
     source          1..669
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(500..519)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atgatgacaa atctagaccc ggggagcgcc ccttccagtg tcgcatttgc atgcggaact
       61 tttcggcacc atctaaattg gcacgtcata cccgtactca taccggtgaa aaaccgtttc
      121 agtgtcggat ctgtatgcga aatttctccc agcgttctga cttgacccgt catctacgta
      181 cgcacaccgg cgagaagcca ttccaatgcc gaatatgcat gcgcaacttc agtgacccat
      241 ctaacttgcg tcgtcaccta aaaacccacc tgaggggatc ctctgcacag tagtaactag
      301 agactagaaa aaggccgaca agtcccgctc cgctgaagat cctggcgtaa tagcgaagag
      361 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
      421 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
      481 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
      541 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
      601 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg ggccatcgcc
      661 ctgatagac
//
