LOCUS       sequence-442401-        9343 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposon plasmid for doxycycline inducible expression of 
            MEIOC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9343)
  AUTHORS   Pierson Smela M, Adams J, Ma C, Breimann L, Widocki U, Shioda T, 
            Church GM
  TITLE     Induction of Meiosis from Human Pluripotent Stem Cells
  JOURNAL   bioRxiv 2024.05.31.596483
REFERENCE   2  (bases 1 to 9343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.31.596483"
COMMENT     Sequence Label: pPB-cT3G-cERP2-MEIOC
FEATURES             Location/Qualifiers
     source          1..9343
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    53..71
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    89..107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    197..215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    233..251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     278..302
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    368..392
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      393..402
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3258..3282)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3284..3310
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    3477..3611
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   3883..3917
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(3928..3945)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3928..3944)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3937..3959)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4153..4172)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4274..4296
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4335..4353)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4421..4525
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4526..4594,4595..5386)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4744..4763)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5557..6145
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6046..6065
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6299..6316
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6522..6543
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6558..6563,6564..6581,6582..6588)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6596..6612
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6601..6623
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6620..6636
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6620..6636
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   6662..6724
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    6900..6981
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(7698..7754)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(7764..8507)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(8508..8531)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      8531..8540
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(8560..8771)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        8772..9151
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
       61 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
      121 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
      181 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
      241 agtgatagag aacgtataag cttgcctata taagcagagc tcgtttagtg aaccgtcaga
      301 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      361 gcctaccaca agtttgtaca aaaaagcagg ctgccaccat ggaagttaga cgcggagata
      421 catgcccaag accccatcct tccgggcttc gggaagaagg acttgagccc aaagtcgcgt
      481 tccccggagg tgcgaatcgc tgttggaacc tcggcgccga cgccggcagc aggttaaccg
      541 acgtcttcgg cagcgtgatg ttgactggct ccgcttcctt ctacgattgc tacacatcgc
      601 agagtgaaga caatgtagac ctaaggcaga cctatactcc attttcttca acagaatatt
      661 caagttctgt agattcttca cttttctgtg caccatggtc tacttatgga gatgacatta
      721 aacaaccttc taattctcag atcagtataa agaacaggat tcaaacagaa agaaatgact
      781 atggcagtga aacagactta tatggacttg tgtctaacat tttggaagaa caagataagt
      841 cacagccata ttttgctgaa gggacctgct cctccaattt aaagtcagtt tggccaatga
      901 acacaagcag atttgcagat caccatgacc tcttaacaga aaccaaaagg ccaatagata
      961 cagtcatctc tcagcaagct ttttatagtg atgaatctgt gtcagcaatg gaaaagcaat
     1021 acctgcgtaa cagtaatctc acaccacaac aaaaaataga tgaacttcat catggattta
     1081 ctggtttaga tcttgaagaa caatggatgt acccctcacg aagtgatcat tctaactgtc
     1141 acaatattca gacaaatgat acagctaaga caacattcca agaatatcca cttatcaaaa
     1201 actgttttac accccaaact ggtctgtctg atatcatgaa agaatcagga gttgatatct
     1261 accattatgg aagagacaga atatgtacta aaggtcttga agcaccacta cagcaaaaaa
     1321 gggcagagat gttcctcagt cagttcaacc gatataacga gaacgtggac tactgccgct
     1381 acccagagta tgttcatcct aataaggcta agcttaataa atgttcaaat tttagtgtcc
     1441 aagatagcaa aaaattagcc aatggcacac ctgaaacacc aactgtagaa gcagacacct
     1501 acacaaagtt atttcaggtt aagccagcga atcagaaaaa aatggaggag acaatccctg
     1561 atcagcagaa tttcacattt ccaaaaacta cgccacatct gacagagaaa cagtttgcaa
     1621 aggaagcagt attcactgct gattttggct taacatcaga atatggacta aaacctcaca
     1681 cagcttgtcc cgctaatgat tttgctaacg tcacagaaaa gcaacagttt gctaaacctg
     1741 atcccccaca ttctgagtat tttaaatcag tgaatttatt atcaaactca gcaacatctt
     1801 caggaggtat caatttaaac agaccaactt ggatgaatgt tcaaacaaaa aataacactc
     1861 ctattcctta tcgaaatcaa ggtaacttga tgaaattaaa tagtcattta agtgcagctt
     1921 caaaaggttc taaccattct tcagatttcc cccaactatc atccacaaac ttaaccccaa
     1981 atagcaattt atttcagaaa tattgccaag aaaacccttc agcattttct agttttgatt
     2041 ttagttacag tggtgcagaa agaatccaat ctgtcaatca catagaagga ctaacaaagc
     2101 ctggagaaga aaatctcttc aaattggtta cggataaaaa aataaagcag ccaaatggat
     2161 tttgtgataa ctattcagct cagaagtatg ggataattga aaatgtaaac aaacataatt
     2221 ttcaagccaa gccccagagt ggacattatg atcctgagga aggtccaaag catttagatg
     2281 gcttatcaca aaatacatac caagatctac tggagtcaca gggtcattct aatagccaca
     2341 gaacgagagg tggagacaat agccgtgtga atcgcacaca agtgtcatgc ttttctaata
     2401 attatatgat gggagattta aggcataatc agtgttttca acaacttggt tcaaatgggt
     2461 ttcccctaag atccacccac ccatttggcc attcagttgt tccactgttg gattcctatg
     2521 acttactttc ttatgatgac ttaagccatt tgtaccctta ttttaatatg atgtatggtg
     2581 ataattcttt ttctggtctc atgccaactt ttggatttca aagaccaatt aaaacccgta
     2641 gtggaccagc cagtgaactt catattcgtc tagaagagtg ctgtgaacaa tggagagcat
     2701 tagaaaaaga gagaaaaaag actgaattag cccttgccaa gaattatcct ggaaaaaaag
     2761 tatccagtac taacaacact ccagttccaa ggctgacttc caacccatct agagttgatc
     2821 gcttaattgt ggatgaactt cgagaactag ccagagttgt gactttacta ggcaaaatgg
     2881 aacgtcttcg gagttctctt cttcatgcca gtatctctac tgctcttgat agacacttgg
     2941 agtctattca cattgtacag tcacgtagaa aggatgaaat tgttaatgct tccaatcggc
     3001 aaaggcaagg agttcctaga tgccaagatg acagagatgt ttttgccctt gcttctgcaa
     3061 ttaaagagat gtgtgtggct actcggaaaa cacgcactgc cctgtggtgt gcactgcaga
     3121 tgaccttgcc aaaaacagcc agtacagctg atgtggtaaa gcctttacaa gatacagtaa
     3181 actgtgaaga taaagtccat gaaagcataa atagtagcaa tccaatgaac cagagaggtg
     3241 aaacaaacaa acattaaacc cagctttctt gtacaaagtg gtatacccat acgatgttcc
     3301 agattacgct tagtaatgag aaatactcag aagatgtcac tcaccatagt ccgcagtgaa
     3361 agataaatga tcgattttga gtacgattac tcgttttaga gctagaaata gcaagtaggc
     3421 cactgagcaa gaactatgaa tgagatcttt agacatattg cggccgcttt ctccgcgatc
     3481 cagacatgat aagatacatt gatgagtttg gacaaaccaa aactagaatg cagtgaaaaa
     3541 aatgccttat ttgtgaaatt tgtgatgcta ttgccttatt tgtaaccatt ataagctgca
     3601 ataaacaagt tatctataac aagaaaatat atatataata agttatcacg taagtagaac
     3661 atgaaataac aatataatta tcgtatgagt taaatcttaa aagtcacgta aaagataatc
     3721 atgcgtcatt ttgactcacg cggtcgttat agttcaaaat cagtgacact taccgcattg
     3781 acaagcacgc ctcacgggag ctccaagcgg cgactgagat gtcctaaatg cacagcgacg
     3841 gattcgcgct atttagaaag agagagcaat atttcaagaa tgcatgcgtc aattttacgc
     3901 agactatctt tctagggtta agaattcact ggccgtcgtt ttacaacgtc gtgactggga
     3961 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     4021 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     4081 atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     4141 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     4201 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     4261 tgtgaccatg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacac
     4321 gcgagttcga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     4381 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     4441 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     4501 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     4561 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     4621 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     4681 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     4741 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     4801 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     4861 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     4921 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     4981 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     5041 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     5101 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     5161 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     5221 atctggagcc ggtgagcgtg gttcacgcgg tatcattgca gcactggggc cagatggtaa
     5281 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     5341 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     5401 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     5461 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     5521 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     5581 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     5641 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     5701 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     5761 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     5821 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     5881 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     5941 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6001 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6061 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6121 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6181 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     6241 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     6301 cgagtcagtg agcgaggaag cggatgagcg cccaatacgc aaaccgcctc tcaccgcgcg
     6361 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     6421 gcgcaacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     6481 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     6541 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     6601 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa ggtcgactta
     6661 accctagaaa gataatcata ttgtgacgta cgttaaagat aatcatgcgt aaaattgacg
     6721 catgtgtttt atcggtctgt atatcgaggt ttatttatta atttgaatag atattaagtt
     6781 ttattatatt tacacttaca tactaataat aaattcaaca aacaatttat ttatgtttat
     6841 ttatttatta aaaaaaaaca aaaactcaaa atttcttcta taaagtaaca aaacttttag
     6901 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     6961 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     7021 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat
     7081 tatgatcctc tggagattca tgcgccaggt tttctggtca tgcaccacgt tcgtgggccc
     7141 tcaggaacct ccacgtcagc ggtaactgtg aagcccagtc gctcgtaaaa aggaagattc
     7201 cggggagcac tggtttcgag gaacgccggc acgccagccc gttccgcggc ctcaacacca
     7261 ggaagaacga cagcgcttcc caaaccttta ccttggtgat ctgggctgac accgacagtg
     7321 gcaagaaacc acgcaggctc ctttggtcta tgaggagcca gcaagccctc catttgctgt
     7381 tgtgccgcca acctactccc tgacagctcg gccatccgcg gacctatttc cgcgaacact
     7441 gcgccggctt cgacgctttc cggagtagtc cagaccgcaa ctgcggcacc atcgtccgca
     7501 acccaaactt taccaatgtc cagacccacg cgcgtaagaa acagctcttg caactcagtt
     7561 accctctcta tgtgtctgtc cggatctacg gtatggcggg tcgctggata atctgcaaat
     7621 gctgcggcaa gtgttcggac agcccgtggt acatcgtcac gggtagcgag cctaacagtg
     7681 ggcttatatt ccgtcatggg gccggggttc tcctccacgt cgccggcctg cttcagcagg
     7741 ctgaagttgg tggcgccgct gcccccgggg agcatgtcaa ggtcaaaatc gtcaagagcg
     7801 tcagcaggca gcatatcaag gtcaaagtcg tcaagggcat cggctgggag catgtctaag
     7861 tcaaaatcgt caagggcgtc ggtcggcccg ccgctttcgc actttagctg tttctccagg
     7921 ccacatatga ttagttccag gccgaaaagg aaggcaggtt cggctccctg ccggtcgaac
     7981 agctcaattg cttgtttcag aagtgggggc atagaatcgg tggtaggtgt ctctctttcc
     8041 tcttttgcta cttgatgctc ctgttcctcc aatacgcagc ccagtgtaaa gtggcccacg
     8101 gcggacagag cgtacagtgc gttctccagg gagaagcctt gctgacacag gaacgcgagc
     8161 tgattttcca gggtttcgta ctgtttctct gttgggcggg tgccgagatg cactttagcc
     8221 ccgtcacgat gtgagaggag agcacagcgg tatgacttgg cgttgttccg cagaaagtct
     8281 tgccatgact cgccttccag ggggcaggag tgggtatgat gcctgtccag catctcgatt
     8341 ggcagggcat cgagcagggc ccgcttgttc ttcacgtgcc agtacagggt aggctgctca
     8401 actcccagct tttgagcgag tttccttgtc gtcaggcctt cgataccgac tccattgagt
     8461 aattccagag cagagtttat gactttgctc ttgtccagtc tagacatctt atcgtcatcg
     8521 tctttgtaat ccatggtggc ggatcccgcg tcacgacacc tgtgttctgg cggcaaaccc
     8581 gttgcgaaaa agaacgttca cggcgactac tgcacttata tacggttctc ccccaccctc
     8641 gggaaaaagg cggagccagt acacgacatc actttcccag tttaccccgc gccaccttct
     8701 ctaggcaccg gttcaattgc cgacccctcc ccccaacttc tcgggcactg tgggccatgt
     8761 gcgctctgcc ccatggtaat agccatgact aatacgtaga tgtactgcca agtaggaaag
     8821 tgccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca
     8881 atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt
     8941 aaatactcca cccattgacg tcaatggaaa gtgcctattg gcgttactat gggaacatac
     9001 gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc
     9061 gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat
     9121 tactattaat aactagtcaa taatcaatgt cataagctct aatcgcataa tcgagctaag
     9181 agcgattaat atgcttagct agcttagcta agctagattt ctctattctc ccacaatata
     9241 tagcagctat tagcttagca gtccgaatac gatcgtccac gatccataag cgtaacacat
     9301 ttgagtagcg actgacgggc accggagcca ctcgagtgga att
//
