LOCUS       sequence-442454-        8572 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposon plasmid for doxycycline inducible expression of 
            CTCFL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Pierson Smela M, Adams J, Ma C, Breimann L, Widocki U, Shioda T, 
            Church GM
  TITLE     Induction of Meiosis from Human Pluripotent Stem Cells
  JOURNAL   bioRxiv 2024.05.31.596483
REFERENCE   2  (bases 1 to 8572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.31.596483"
COMMENT     Sequence Label: pPB-cT3G-cERP2-CTCFL
FEATURES             Location/Qualifiers
     source          1..8572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    16..34
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    52..70
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    88..106
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    124..142
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    160..178
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    196..214
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    232..250
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     277..301
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    367..391
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2486..2510)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2512..2538
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    2705..2839
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   3111..3145
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(3156..3173)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3156..3172)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3165..3187)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3381..3400)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3502..3524
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3563..3581)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3649..3753
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3754..3822,3823..4614)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3972..3991)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4785..5373
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5274..5293
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5527..5544
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5750..5771
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5786..5791,5792..5809,5810..5816)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5824..5840
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5829..5851
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5848..5864
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5848..5864
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   5890..5952
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    6128..6209
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(6926..6982)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(6992..7735)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(7736..7759)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      7759..7768
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7788..7999)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        8000..8379
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tcacctcgag tttactccct atcagtgata gagaacgtat gaagagttta ctccctatca
       61 gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt ataaggagtt
      121 tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt gatagagaac
      181 gtatctacag tttactccct atcagtgata gagaacgtat atccagttta ctccctatca
      241 gtgatagaga acgtataagc ttgcctatat aagcagagct cgtttagtga accgtcagat
      301 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
      361 cctaccacaa gtttgtacaa aaaagttggc accatggcag ccactgagat ctctgtcctt
      421 tctgagcaat tcaccaagat caaagaactc gagttgatgc cggaaaaagg cctgaaggag
      481 gaggaaaaag acggagtgtg cagagagaaa gaccatcgga gccctagtga gttggaggcc
      541 gagcgtacct ctggggcctt ccaggacagc gtcctggagg aagaagtgga gctggtgctg
      601 gccccctcgg aggagagcga gaagtacatc ctgaccctgc agacggtgca cttcacttct
      661 gaagctgtgg agttgcagga tatgagcttg ctgagcatac agcagcaaga aggggtgcag
      721 gtggtggtgc aacagcctgg ccctgggttg ctgtggcttg aggaagggcc ccggcagagc
      781 ctgcagcagt gtgtggccat tagtatccag caagagctgt actccccgca agagatggag
      841 gtgttgcagt tccacgctct agaggagaat gtgatggtgg ccagtgaaga cagtaagtta
      901 gcggtgagcc tggctgaaac tgctggactg atcaagctcg aggaagagca ggagaagaac
      961 cagttattgg ctgaaagaac aaaggagcag ctcttttttg tggaaacaat gtcaggagat
     1021 gaaagaagtg acgaaattgt tctcacagtt tcaaattcaa atgtggaaga acaagaggat
     1081 caacctacag ctggtcaagc agatgctgaa aaggccaaat ctacaaaaaa tcaaagaaag
     1141 acaaagggag caaaaggaac cttccactgt gatgtctgca tgttcacctc ttctagaatg
     1201 tcaagtttta atcgtcatat gaaaactcac accagtgaga agcctcacct gtgtcacctc
     1261 tgcctgaaaa ccttccgtac ggtcactctg ctgcggaacc atgttaacac ccacacagga
     1321 accaggccct acaagtgtaa cgactgcaac atggcatttg tcaccagtgg agaactcgtc
     1381 cgacacaggc gctataaaca tactcatgag aaacccttta aatgttccat gtgcaagtat
     1441 gccagtgtgg aggcaagtaa attgaagcgc catgtccgat cccacactgg ggagcgcccc
     1501 tttcagtgtt gccagtgcag ctatgccagc agagacacct acaagctgaa acgccacatg
     1561 agaacgcact caggtgagaa gccttacgaa tgccacatct gccacacccg cttcacccag
     1621 agcgggacca tgaaaataca tattctgcag aaacacggcg aaaatgtccc caaataccag
     1681 tgtccccatt gtgccaccat cattgcacgg aaaagcgacc tacgtgtgca tatgcgcaac
     1741 ttgcatgctt acagcgctgc agagctgaaa tgccgctact gttctgctgt cttccatgaa
     1801 cgctatgccc tcattcagca ccagaaaact cataagaatg agaagaggtt caagtgcaaa
     1861 cactgcagtt atgcctgcaa gcaggaacgt catatgaccg ctcacattcg tacccacact
     1921 ggagagaaac cattcacctg cctttcttgc aataaatgtt tccgacagaa gcaacttcta
     1981 aacgctcact tcaggaaata ccacgatgca aatttcatcc cgactgttta caaatgctcc
     2041 aagtgtggca aaggcttttc ccgctggatt aacctgcaca gacattcgga gaagtgtgga
     2101 tcaggggaag caaagtcggc tgcttcagga aagggaagaa gaacaagaaa gaggaagcag
     2161 accatcctga aggaagccac aaagggtcag aaggaagctg cgaagggatg gaaggaagcc
     2221 gcgaacggag acgaagctgc tgctgaggag gcttccacca cgaagggaga acagttccca
     2281 ggagagatgt ttcctgtcgc ctgcagagaa accacagcca gagtcaaaga ggaagtggat
     2341 gaaggcgtga cctgtgaaat gctcctcaac acgatggata agtgagaggg attcgggttg
     2401 cgtgttcact gcccccaatt cctaaagcaa gttagaagtt tttagcattt aaggtgtgaa
     2461 atgctcctca acacgatgga taagttgcca actttcttgt acaaagtggt atacccatac
     2521 gatgttccag attacgctta gtaatgagaa atactcagaa gatgtcactc accatagtcc
     2581 gcagtgaaag ataaatgatc aagaggcact tgcactgtca gttttagagc tagaaatagc
     2641 aagtaggcca ctgagcaaga actatgaatg agatctttag acatattgcg gccgctttct
     2701 ccgcgatcca gacatgataa gatacattga tgagtttgga caaaccaaaa ctagaatgca
     2761 gtgaaaaaaa tgccttattt gtgaaatttg tgatgctatt gccttatttg taaccattat
     2821 aagctgcaat aaacaagtta tctataacaa gaaaatatat atataataag ttatcacgta
     2881 agtagaacat gaaataacaa tataattatc gtatgagtta aatcttaaaa gtcacgtaaa
     2941 agataatcat gcgtcatttt gactcacgcg gtcgttatag ttcaaaatca gtgacactta
     3001 ccgcattgac aagcacgcct cacgggagct ccaagcggcg actgagatgt cctaaatgca
     3061 cagcgacgga ttcgcgctat ttagaaagag agagcaatat ttcaagaatg catgcgtcaa
     3121 ttttacgcag actatctttc tagggttaag aattcactgg ccgtcgtttt acaacgtcgt
     3181 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     3241 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     3301 aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     3361 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     3421 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     3481 agacaagctg tgaccatgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     3541 cgaaacacgc gagttcgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     3601 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     3661 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     3721 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     3781 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     3841 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     3901 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     3961 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     4021 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     4081 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     4141 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     4201 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     4261 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     4321 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     4381 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     4441 gctgataaat ctggagccgg tgagcgtggt tcacgcggta tcattgcagc actggggcca
     4501 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     4561 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     4621 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     4681 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     4741 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     4801 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     4861 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     4921 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     4981 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5041 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5101 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5161 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5221 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5281 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     5341 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     5401 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     5461 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     5521 gagcgcagcg agtcagtgag cgaggaagcg gatgagcgcc caatacgcaa accgcctctc
     5581 accgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     5641 ggcagtgagc gcaacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     5701 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     5761 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     5821 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagg
     5881 tcgacttaac cctagaaaga taatcatatt gtgacgtacg ttaaagataa tcatgcgtaa
     5941 aattgacgca tgtgttttat cggtctgtat atcgaggttt atttattaat ttgaatagat
     6001 attaagtttt attatattta cacttacata ctaataataa attcaacaaa caatttattt
     6061 atgtttattt atttattaaa aaaaaacaaa aactcaaaat ttcttctata aagtaacaaa
     6121 acttttagca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     6181 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     6241 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     6301 tggctgatta tgatcctctg gagattcatg cgccaggttt tctggtcatg caccacgttc
     6361 gtgggccctc aggaacctcc acgtcagcgg taactgtgaa gcccagtcgc tcgtaaaaag
     6421 gaagattccg gggagcactg gtttcgagga acgccggcac gccagcccgt tccgcggcct
     6481 caacaccagg aagaacgaca gcgcttccca aacctttacc ttggtgatct gggctgacac
     6541 cgacagtggc aagaaaccac gcaggctcct ttggtctatg aggagccagc aagccctcca
     6601 tttgctgttg tgccgccaac ctactccctg acagctcggc catccgcgga cctatttccg
     6661 cgaacactgc gccggcttcg acgctttccg gagtagtcca gaccgcaact gcggcaccat
     6721 cgtccgcaac ccaaacttta ccaatgtcca gacccacgcg cgtaagaaac agctcttgca
     6781 actcagttac cctctctatg tgtctgtccg gatctacggt atggcgggtc gctggataat
     6841 ctgcaaatgc tgcggcaagt gttcggacag cccgtggtac atcgtcacgg gtagcgagcc
     6901 taacagtggg cttatattcc gtcatggggc cggggttctc ctccacgtcg ccggcctgct
     6961 tcagcaggct gaagttggtg gcgccgctgc ccccggggag catgtcaagg tcaaaatcgt
     7021 caagagcgtc agcaggcagc atatcaaggt caaagtcgtc aagggcatcg gctgggagca
     7081 tgtctaagtc aaaatcgtca agggcgtcgg tcggcccgcc gctttcgcac tttagctgtt
     7141 tctccaggcc acatatgatt agttccaggc cgaaaaggaa ggcaggttcg gctccctgcc
     7201 ggtcgaacag ctcaattgct tgtttcagaa gtgggggcat agaatcggtg gtaggtgtct
     7261 ctctttcctc ttttgctact tgatgctcct gttcctccaa tacgcagccc agtgtaaagt
     7321 ggcccacggc ggacagagcg tacagtgcgt tctccaggga gaagccttgc tgacacagga
     7381 acgcgagctg attttccagg gtttcgtact gtttctctgt tgggcgggtg ccgagatgca
     7441 ctttagcccc gtcacgatgt gagaggagag cacagcggta tgacttggcg ttgttccgca
     7501 gaaagtcttg ccatgactcg ccttccaggg ggcaggagtg ggtatgatgc ctgtccagca
     7561 tctcgattgg cagggcatcg agcagggccc gcttgttctt cacgtgccag tacagggtag
     7621 gctgctcaac tcccagcttt tgagcgagtt tccttgtcgt caggccttcg ataccgactc
     7681 cattgagtaa ttccagagca gagtttatga ctttgctctt gtccagtcta gacatcttat
     7741 cgtcatcgtc tttgtaatcc atggtggcgg atcccgcgtc acgacacctg tgttctggcg
     7801 gcaaacccgt tgcgaaaaag aacgttcacg gcgactactg cacttatata cggttctccc
     7861 ccaccctcgg gaaaaaggcg gagccagtac acgacatcac tttcccagtt taccccgcgc
     7921 caccttctct aggcaccggt tcaattgccg acccctcccc ccaacttctc gggcactgtg
     7981 ggccatgtgc gctctgcccc atggtaatag ccatgactaa tacgtagatg tactgccaag
     8041 taggaaagtg ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat
     8101 tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag
     8161 tttaccgtaa atactccacc cattgacgtc aatggaaagt gcctattggc gttactatgg
     8221 gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc
     8281 catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg
     8341 taattgatta ctattaataa ctagtcaata atcaatgtca taagctctaa tcgcataatc
     8401 gagctaagag cgattaatat gcttagctag cttagctaag ctagatttct ctattctccc
     8461 acaatatata gcagctatta gcttagcagt ccgaatacga tcgtccacga tccataagcg
     8521 taacacattt gagtagcgac tgacgggcac cggagccact cgagtggaat ta
//
