LOCUS       sequence-442466-        9271 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposon plasmid for doxycycline inducible expression of 
            RB1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9271)
  AUTHORS   Pierson Smela M, Adams J, Ma C, Breimann L, Widocki U, Shioda T, 
            Church GM
  TITLE     Induction of Meiosis from Human Pluripotent Stem Cells
  JOURNAL   bioRxiv 2024.05.31.596483
REFERENCE   2  (bases 1 to 9271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.31.596483"
COMMENT     Sequence Label: pPB-cT3G-cERP2-RB1
FEATURES             Location/Qualifiers
     source          1..9271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    53..71
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    89..107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    197..215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    233..251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     278..302
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    368..392
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3186..3210)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3212..3238
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    3405..3539
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   3811..3845
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(3856..3873)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3856..3872)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3865..3887)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4081..4100)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4202..4224
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4263..4281)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4349..4453
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4454..4522,4523..5314)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4672..4691)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5485..6073
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5974..5993
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6227..6244
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6450..6471
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6486..6491,6492..6509,6510..6516)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6524..6540
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6529..6551
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6548..6564
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6548..6564
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   6590..6652
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    6828..6909
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(7626..7682)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(7692..8435)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(8436..8459)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      8459..8468
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(8488..8699)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        8700..9079
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
       61 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
      121 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
      181 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
      241 agtgatagag aacgtataag cttgcctata taagcagagc tcgtttagtg aaccgtcaga
      301 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      361 gcctaccaca agtttgtaca aaaaagcagg ctgccaccat gccaccgaag acaccaagga
      421 agactgcggc aactgctgct gccgcagctg ccgagccacc agctcctcct ccacctcctc
      481 ccccagaaga agatcctgaa caagattctg ggccagaaga tcttcccctg gtaagactcg
      541 aattcgagga gactgaggag cccgacttca cagctctgtg ccaaaagctc aaaattcctg
      601 accacgttcg agaacgagca tggctgacat gggaaaaggt cagctccgtc gacggtgtcc
      661 tcggcggcta catacagaag aagaaagagc tctggggcat atgcattttc atcgccgctg
      721 tcgatttaga cgaaatgtct tttaccttca ccgaattgca aaagaatatc gagatttctg
      781 tgcacaagtt cttcaatctg ctgaaggaga tagacacttc tacaaaggtc gacaacgcca
      841 tgagtcggct tctgaagaaa tacgacgtgc tcttcgcctt attttctaag ctggagcgaa
      901 cttgcgagct gatttacctg acgcagcctt cttcaagcat ctccacagag atcaacagtg
      961 ctctggttct caaggtcagc tggattactt tcctgcttgc aaagggtgag gtcctgcaga
     1021 tggaagacga ccttgttatc agtttccaat tgatgttgtg cgttttggat tacttcatca
     1081 agctttctcc gccgatgctc ctgaaggagc cctacaagac tgccgtaatc ccaatcaacg
     1141 gcagcccacg tactcctcgt cggggccaaa atcgtagcgc ccgcatcgcc aagcagctcg
     1201 agaacgacac tcgcataatc gaggtcctgt gcaaggagca cgagtgcaac atcgacgaag
     1261 ttaagaacgt gtactttaag aacttcattc cattcatgaa ctcactcggc ttagtgacct
     1321 caaacggtct gcctgaagtc gagaacctgt ccaagaggta tgaggagatc tacctgaaga
     1381 acaaggacct cgacgctcgg ctgttcctcg accacgacaa gacactgcaa acagactcaa
     1441 ttgatagctt cgagacgcaa cgaaccccca ggaagtccaa tctcgacgag gaagtcaacg
     1501 tcataccccc tcatacgcct gtgcggacgg tgatgaatac catacagcag ctgatgatga
     1561 tactgaactc tgcatccgac cagccgtctg agaaccttat aagttacttc aataattgta
     1621 ccgtcaaccc caaggagagc attttgaagc gggttaaaga cattggttat atattcaagg
     1681 aaaagttcgc aaaggccgtc ggtcaaggat gcgtggagat aggttcccaa cgttataagt
     1741 tgggtgtgag gctttactat agagtgatgg agtcaatgct gaagagcgag gaagagcggc
     1801 tgagtatcca gaacttctcc aagctcctca acgataatat cttccacatg tccttgctgg
     1861 catgtgcact ggaagtggtt atggcgacct actcaaggtc tactagccaa aacctggact
     1921 ccggcactga cctcagtttt ccttggatcc tcaacgtcct gaaccttaag gctttcgact
     1981 tctataaggt aattgagagc ttcattaagg ctgagggaaa tctcaccagg gagatgatta
     2041 agcacttgga gagatgcgag caccggataa tggagtctct ggcttggctt agtgacagcc
     2101 cactgttcga cctgatcaag cagagcaaag atagagaggg tccgaccgac catttagaga
     2161 gtgcctgccc cctcaacctg ccattgcaaa acaaccatac cgccgccgac atgtacctgt
     2221 cccccgtgag gtcacccaag aagaagggca gtaccacacg cgttaacagt acagctaacg
     2281 cggaaactca ggctacttcc gcatttcaaa ctcagaaacc gctgaagagc acaagcctga
     2341 gccttttcta caagaaggtt taccgcttgg cttacctgcg actgaacacc ttgtgcgaga
     2401 ggctgctcag cgaacatcct gagttggagc acataatttg gacactcttt caacatacat
     2461 tacaaaacga atacgagttg atgagggatc gccaccttga tcagatcatg atgtgcagta
     2521 tgtacgggat ctgtaaggtt aagaacatcg atctgaagtt taagataatc gtgactgctt
     2581 ataaagactt accacacgcc gtgcaagaaa cctttaagcg ggtgctgatt aaagaggaag
     2641 aatacgactc aataattgtc ttttacaatt ctgtgtttat gcaaaggctt aagacgaaca
     2701 tccttcaata cgcctctaca agaccaccca ctctcagccc cattccccat atcccacgct
     2761 ctccctataa attcccaagc tctccactgc gcatcccggg tggtaatatt tacatcagtc
     2821 cgttgaaaag cccctacaag ataagcgagg gcctgcccac cccgacgaag atgacaccgc
     2881 ggtcccgcat acttgtcagc atcggagagt cctttggcac atcagaaaag tttcaaaaga
     2941 ttaaccaaat ggtctgcaat tctgatagag tactgaagcg ctctgccgag ggctctaatc
     3001 ccccaaagcc tttgaagaag ctgaggttcg acatcgaggg gagtgacgag gccgacgggt
     3061 ctaagcacct tcctggggaa agcaagttcc aacaaaagct tgccgagatg acttccaccc
     3121 gcaccaggat gcagaaacaa aagatgaacg actccatgga cacatccaat aaagaggaaa
     3181 agtaaaccca gctttcttgt acaaagtggt atacccatac gatgttccag attacgctta
     3241 gtaatgagaa atactcagaa gatgtcactc accatagtcc gcagtgaaag ataaatgatc
     3301 accttactta aaatcgtttc gttttagagc tagaaatagc aagtaggcca ctgagcaaga
     3361 actatgaatg agatctttag acatattgcg gccgctttct ccgcgatcca gacatgataa
     3421 gatacattga tgagtttgga caaaccaaaa ctagaatgca gtgaaaaaaa tgccttattt
     3481 gtgaaatttg tgatgctatt gccttatttg taaccattat aagctgcaat aaacaagtta
     3541 tctataacaa gaaaatatat atataataag ttatcacgta agtagaacat gaaataacaa
     3601 tataattatc gtatgagtta aatcttaaaa gtcacgtaaa agataatcat gcgtcatttt
     3661 gactcacgcg gtcgttatag ttcaaaatca gtgacactta ccgcattgac aagcacgcct
     3721 cacgggagct ccaagcggcg actgagatgt cctaaatgca cagcgacgga ttcgcgctat
     3781 ttagaaagag agagcaatat ttcaagaatg catgcgtcaa ttttacgcag actatctttc
     3841 tagggttaag aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     3901 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     3961 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
     4021 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     4081 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     4141 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccatgtc
     4201 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacacgc gagttcgaaa
     4261 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     4321 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     4381 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     4441 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     4501 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     4561 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     4621 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     4681 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     4741 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     4801 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     4861 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     4921 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     4981 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     5041 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     5101 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     5161 tgagcgtggt tcacgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     5221 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     5281 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     5341 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5401 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5461 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5521 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5581 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     5641 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5701 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5761 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5821 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5881 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5941 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6001 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6061 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6121 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     6181 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     6241 cgaggaagcg gatgagcgcc caatacgcaa accgcctctc accgcgcgtt ggccgattca
     6301 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaaa
     6361 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     6421 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     6481 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     6541 tttcacacag gaaacagcta tgaccatgat tacgccaagg tcgacttaac cctagaaaga
     6601 taatcatatt gtgacgtacg ttaaagataa tcatgcgtaa aattgacgca tgtgttttat
     6661 cggtctgtat atcgaggttt atttattaat ttgaatagat attaagtttt attatattta
     6721 cacttacata ctaataataa attcaacaaa caatttattt atgtttattt atttattaaa
     6781 aaaaaacaaa aactcaaaat ttcttctata aagtaacaaa acttttagca gtgaaaaaaa
     6841 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     6901 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     6961 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcctctg
     7021 gagattcatg cgccaggttt tctggtcatg caccacgttc gtgggccctc aggaacctcc
     7081 acgtcagcgg taactgtgaa gcccagtcgc tcgtaaaaag gaagattccg gggagcactg
     7141 gtttcgagga acgccggcac gccagcccgt tccgcggcct caacaccagg aagaacgaca
     7201 gcgcttccca aacctttacc ttggtgatct gggctgacac cgacagtggc aagaaaccac
     7261 gcaggctcct ttggtctatg aggagccagc aagccctcca tttgctgttg tgccgccaac
     7321 ctactccctg acagctcggc catccgcgga cctatttccg cgaacactgc gccggcttcg
     7381 acgctttccg gagtagtcca gaccgcaact gcggcaccat cgtccgcaac ccaaacttta
     7441 ccaatgtcca gacccacgcg cgtaagaaac agctcttgca actcagttac cctctctatg
     7501 tgtctgtccg gatctacggt atggcgggtc gctggataat ctgcaaatgc tgcggcaagt
     7561 gttcggacag cccgtggtac atcgtcacgg gtagcgagcc taacagtggg cttatattcc
     7621 gtcatggggc cggggttctc ctccacgtcg ccggcctgct tcagcaggct gaagttggtg
     7681 gcgccgctgc ccccggggag catgtcaagg tcaaaatcgt caagagcgtc agcaggcagc
     7741 atatcaaggt caaagtcgtc aagggcatcg gctgggagca tgtctaagtc aaaatcgtca
     7801 agggcgtcgg tcggcccgcc gctttcgcac tttagctgtt tctccaggcc acatatgatt
     7861 agttccaggc cgaaaaggaa ggcaggttcg gctccctgcc ggtcgaacag ctcaattgct
     7921 tgtttcagaa gtgggggcat agaatcggtg gtaggtgtct ctctttcctc ttttgctact
     7981 tgatgctcct gttcctccaa tacgcagccc agtgtaaagt ggcccacggc ggacagagcg
     8041 tacagtgcgt tctccaggga gaagccttgc tgacacagga acgcgagctg attttccagg
     8101 gtttcgtact gtttctctgt tgggcgggtg ccgagatgca ctttagcccc gtcacgatgt
     8161 gagaggagag cacagcggta tgacttggcg ttgttccgca gaaagtcttg ccatgactcg
     8221 ccttccaggg ggcaggagtg ggtatgatgc ctgtccagca tctcgattgg cagggcatcg
     8281 agcagggccc gcttgttctt cacgtgccag tacagggtag gctgctcaac tcccagcttt
     8341 tgagcgagtt tccttgtcgt caggccttcg ataccgactc cattgagtaa ttccagagca
     8401 gagtttatga ctttgctctt gtccagtcta gacatcttat cgtcatcgtc tttgtaatcc
     8461 atggtggcgg atcccgcgtc acgacacctg tgttctggcg gcaaacccgt tgcgaaaaag
     8521 aacgttcacg gcgactactg cacttatata cggttctccc ccaccctcgg gaaaaaggcg
     8581 gagccagtac acgacatcac tttcccagtt taccccgcgc caccttctct aggcaccggt
     8641 tcaattgccg acccctcccc ccaacttctc gggcactgtg ggccatgtgc gctctgcccc
     8701 atggtaatag ccatgactaa tacgtagatg tactgccaag taggaaagtg ccataaggtc
     8761 atgtactggg cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta
     8821 cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc
     8881 cattgacgtc aatggaaagt gcctattggc gttactatgg gaacatacgt cattattgac
     8941 gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta
     9001 acgcggaact ccatatatgg gctatgaact aatgaccccg taattgatta ctattaataa
     9061 ctagtcaata atcaatgtca taagctctaa tcgcataatc gagctaagag cgattaatat
     9121 gcttagctag cttagctaag ctagatttct ctattctccc acaatatata gcagctatta
     9181 gcttagcagt ccgaatacga tcgtccacga tccataagcg taacacattt gagtagcgac
     9241 tgacgggcac cggagccact cgagtggaat t
//
