LOCUS       sequence-442472-       10138 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposon plasmid for doxycycline inducible expression of 
            SMC3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10138)
  AUTHORS   Pierson Smela M, Adams J, Ma C, Breimann L, Widocki U, Shioda T, 
            Church GM
  TITLE     Induction of Meiosis from Human Pluripotent Stem Cells
  JOURNAL   bioRxiv 2024.05.31.596483
REFERENCE   2  (bases 1 to 10138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.31.596483"
COMMENT     Sequence Label: pPB-cT3G-cERP2-SMC3
FEATURES             Location/Qualifiers
     source          1..10138
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    53..71
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    89..107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    197..215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    233..251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     278..302
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    368..392
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4053..4077)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4079..4105
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    4272..4406
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   4678..4712
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(4723..4740)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4723..4739)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4732..4754)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4948..4967)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5069..5091
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5130..5148)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5216..5320
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5321..5389,5390..6181)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5539..5558)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6352..6940
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6841..6860
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7094..7111
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7317..7338
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7353..7358,7359..7376,7377..7383)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7391..7407
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7396..7418
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7415..7431
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7415..7431
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   7457..7519
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    7695..7776
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(8493..8549)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(8559..9302)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(9303..9326)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      9326..9335
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(9355..9566)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        9567..9946
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
       61 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
      121 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
      181 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
      241 agtgatagag aacgtataag cttgcctata taagcagagc tcgtttagtg aaccgtcaga
      301 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      361 gcctaccaca agtttgtaca aaaaagcagg ctgccaccat gtacataaag caggtgatta
      421 tccagggttt tcgaagttac agagatcaaa caattgtaga tcccttcagt tcaaaacata
      481 atgtgattgt gggcagaaat ggatctggaa aaagtaactt tttttatgca attcagtttg
      541 ttctcagtga tgagtttagt catcttcgtc cagaacagcg gttggcttta ttgcatgaag
      601 gtactggtcc tcgtgttatt tctgcttttg tggagattat ttttgataat tcagacaacc
      661 ggttaccaat cgataaagag gaagtttcac ttcgaagagt tattggtgcc aaaaaggatc
      721 agtatttctt agacaagaag atggtcacga aaaatgatgt gatgaacctc cttgaaagcg
      781 ctggtttttc tcgaagcaat ccttattata ttgttaaaca aggaaagatc aaccagatgg
      841 caacagcacc agattctcag agattaaagc tattaagaga agtagctggt actagagtgt
      901 atgacgaacg aaaggaagaa agcatctcct taatgaaaga aacagagggc aaacgggaaa
      961 aaatcaatga gttgttaaaa tacattgaag agagattaca tactctagag gaagaaaagg
     1021 aagaactagc tcagtatcag aagtgggata aaatgagacg agccctggaa tataccattt
     1081 acaatcagga acttaacgag actcgtgcca aacttgatga gctttctgct aagcgagaga
     1141 ctagtggaga aaaatccaga caattaagag atgctcagca ggatgcaaga gataaaatgg
     1201 aggatatcga acgccaagtt agagaattga aaacaaaaat ttcagctatg aaagaagaaa
     1261 aagaacagct tagtgctgaa agacaagagc agattaagca gaggactaag ttggagctta
     1321 aagccaagga tttacaagat gaactagcag gcaatagtga acaaaggaaa cgtttattaa
     1381 aagagaggca gaagctgctt gaaaaaatag aagaaaagca gaaagaactg gcagaaacag
     1441 aacccaaatt caacagtgtg aaagagaaag aagaacgagg aattgctaga ttggctcaag
     1501 ctacccagga aagaacggat ctttatgcaa agcagggtcg aggaagccag tttacatcaa
     1561 aagaagaaag ggataagtgg attaaaaagg aactcaagtc tttagatcag gctattaatg
     1621 acaagaaaag acagattgct gctatacata aggatttgga agacactgaa gcaaataaag
     1681 agaaaaatct ggagcagtat aataaactgg accaggatct taatgaagtc aaagctcgag
     1741 tagaagaact ggacagaaaa tattacgaag taaaaaataa gaaagatgaa ctacaaagtg
     1801 aaagaaacta cttgtggaga gaagagaatg cagaacagca agcacttgct gctaaaagag
     1861 aagatcttga aaagaagcaa caacttctta gagcagcaac aggaaaggcc attttaaatg
     1921 gaatagacag cataaacaaa gtgctagacc acttccgtcg aaaaggaata aaccagcatg
     1981 ttcaaaatgg ctatcatggt attgtaatga ataactttga atgtgaacca gctttctaca
     2041 catgcgtgga agtcactgct ggaaacaggt tattttatca cattgttgat tcagatgaag
     2101 tcagcacgaa gattttaatg gagtttaata aaatgaatct tcctggagag gttacttttc
     2161 tgcctcttaa caagttagat gtcagggata cagcctatcc tgaaaccaat gatgctattc
     2221 ctatgatcag caaactgagg tacaatccca gatttgacaa agctttcaaa catgtgtttg
     2281 gaaagactct tatttgtcgt agcatggaag tttcaaccca gctggcccgt gctttcacta
     2341 tggactgtat tactttggaa ggtgaccaag tcagccatcg gggtgctcta actgggggtt
     2401 attatgacac aaggaagtct cgacttgaat tgcaaaaaga tgttagaaaa gcagaagaag
     2461 aactaggtga acttgaagca aagctcaatg aaaacctgcg cagaaatatt gaaaggatta
     2521 ataatgaaat tgatcagttg atgaaccaaa tgcaacagat cgagacccag caaaggaaat
     2581 ttaaagcatc tagagatagc atattatcag aaatgaagat gctaaaagag aagaggcagc
     2641 agtcagagaa aaccttcatg cctaagcaac gtagcttaca gagtttggag gcaagcttgc
     2701 atgctatgga gtctaccaga gagtcattga aagcagaact gggaactgat ttgctttctc
     2761 aactgagttt ggaagatcag aagagagtag atgcactgaa tgatgagatt cgtcaacttc
     2821 agcaggaaaa cagacagttg ctaaatgaaa gaattaaatt agaaggtatt attactcgag
     2881 tagagactta tctcaatgag aatctgagaa aacgcttgga ccaagtagaa caggaactta
     2941 atgagctgag agagacagaa gggggtactg ttctcacagc cacaacatca gaacttgaag
     3001 ccatcaataa aagagtaaaa gacactatgg cacgatcaga agatttggac aattccattg
     3061 ataaaacaga agctggaatt aaggagcttc agaagagtat ggagcgctgg aaaaatatgg
     3121 aaaaagaaca tatggatgct ataaatcatg atactaaaga actggaaaag atgacaaatc
     3181 ggcaaggcat gctattgaag aagaaagaag agtgtatgaa gaaaattcga gaacttggat
     3241 cacttcccca ggaagcattt gaaaagtacc agacactgag cctcaaacag ttgtttcgaa
     3301 aacttgagca gtgcaacaca gaattaaaga agtacagcca tgttaacaaa aaggctttgg
     3361 atcagtttgt aaatttctcc gagcagaaag aaaagttaat aaagcgtcaa gaagagttag
     3421 ataggggtta caaatcaatc atggaactga tgaatgtact tgaacttcgg aaatatgaag
     3481 ctattcagtt aactttcaaa caggtatcta agaacttcag tgaagtattc cagaagttag
     3541 tacctggtgg caaagctact ttggtgatga agaaaggaga tgtggagggc agtcagtctc
     3601 aagatgaagg agaagggagt ggtgagagtg agaggggttc tggctcacaa agcagtgtcc
     3661 catcagttga ccagtttact ggagttggaa ttagggtgtc atttacagga aaacaaggtg
     3721 aaatgagaga aatgcaacag ctttcaggtg gacagaaatc cttggtagcc cttgctctga
     3781 tttttgccat tcagaaatgt gacccggctc cattttactt gtttgatgaa attgaccagg
     3841 ctctggatgc tcagcacaga aaggctgtgt cagatatgat tatggaactt gctgtacatg
     3901 ctcagtttat tacaactact tttaggcctg aactgcttga gtcagctgac aaattctatg
     3961 gtgtaaagtt cagaaataag gttagtcata ttgatgtgat cacagcagag atggccaaag
     4021 actttgtaga agatgatacc acacatggtt aaacccagct ttcttgtaca aagtggtata
     4081 cccatacgat gttccagatt acgcttagta atgagaaata ctcagaagat gtcactcacc
     4141 atagtccgca gtgaaagata aatgatctgc ttgtgagttt ggtggatgtt ttagagctag
     4201 aaatagcaag taggccactg agcaagaact atgaatgaga tctttagaca tattgcggcc
     4261 gctttctccg cgatccagac atgataagat acattgatga gtttggacaa accaaaacta
     4321 gaatgcagtg aaaaaaatgc cttatttgtg aaatttgtga tgctattgcc ttatttgtaa
     4381 ccattataag ctgcaataaa caagttatct ataacaagaa aatatatata taataagtta
     4441 tcacgtaagt agaacatgaa ataacaatat aattatcgta tgagttaaat cttaaaagtc
     4501 acgtaaaaga taatcatgcg tcattttgac tcacgcggtc gttatagttc aaaatcagtg
     4561 acacttaccg cattgacaag cacgcctcac gggagctcca agcggcgact gagatgtcct
     4621 aaatgcacag cgacggattc gcgctattta gaaagagaga gcaatatttc aagaatgcat
     4681 gcgtcaattt tacgcagact atctttctag ggttaagaat tcactggccg tcgttttaca
     4741 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     4801 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     4861 cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     4921 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     4981 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     5041 cgcttacaga caagctgtga ccatgtctcc gggagctgca tgtgtcagag gttttcaccg
     5101 tcatcaccga aacacgcgag ttcgaaaggg cctcgtgata cgcctatttt tataggttaa
     5161 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     5221 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5281 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5341 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     5401 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     5461 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     5521 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     5581 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     5641 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     5701 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     5761 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     5821 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     5881 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     5941 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     6001 gtttattgct gataaatctg gagccggtga gcgtggttca cgcggtatca ttgcagcact
     6061 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     6121 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6181 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6241 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6301 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6361 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6421 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6481 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6541 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6601 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6661 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6721 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6781 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6841 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     6901 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     6961 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     7021 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     7081 aacgaccgag cgcagcgagt cagtgagcga ggaagcggat gagcgcccaa tacgcaaacc
     7141 gcctctcacc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     7201 gaaagcgggc agtgagcgca acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     7261 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     7321 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     7381 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     7441 gccaaggtcg acttaaccct agaaagataa tcatattgtg acgtacgtta aagataatca
     7501 tgcgtaaaat tgacgcatgt gttttatcgg tctgtatatc gaggtttatt tattaatttg
     7561 aatagatatt aagttttatt atatttacac ttacatacta ataataaatt caacaaacaa
     7621 tttatttatg tttatttatt tattaaaaaa aaacaaaaac tcaaaatttc ttctataaag
     7681 taacaaaact tttagcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     7741 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     7801 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     7861 tgtggtatgg ctgattatga tcctctggag attcatgcgc caggttttct ggtcatgcac
     7921 cacgttcgtg ggccctcagg aacctccacg tcagcggtaa ctgtgaagcc cagtcgctcg
     7981 taaaaaggaa gattccgggg agcactggtt tcgaggaacg ccggcacgcc agcccgttcc
     8041 gcggcctcaa caccaggaag aacgacagcg cttcccaaac ctttaccttg gtgatctggg
     8101 ctgacaccga cagtggcaag aaaccacgca ggctcctttg gtctatgagg agccagcaag
     8161 ccctccattt gctgttgtgc cgccaaccta ctccctgaca gctcggccat ccgcggacct
     8221 atttccgcga acactgcgcc ggcttcgacg ctttccggag tagtccagac cgcaactgcg
     8281 gcaccatcgt ccgcaaccca aactttacca atgtccagac ccacgcgcgt aagaaacagc
     8341 tcttgcaact cagttaccct ctctatgtgt ctgtccggat ctacggtatg gcgggtcgct
     8401 ggataatctg caaatgctgc ggcaagtgtt cggacagccc gtggtacatc gtcacgggta
     8461 gcgagcctaa cagtgggctt atattccgtc atggggccgg ggttctcctc cacgtcgccg
     8521 gcctgcttca gcaggctgaa gttggtggcg ccgctgcccc cggggagcat gtcaaggtca
     8581 aaatcgtcaa gagcgtcagc aggcagcata tcaaggtcaa agtcgtcaag ggcatcggct
     8641 gggagcatgt ctaagtcaaa atcgtcaagg gcgtcggtcg gcccgccgct ttcgcacttt
     8701 agctgtttct ccaggccaca tatgattagt tccaggccga aaaggaaggc aggttcggct
     8761 ccctgccggt cgaacagctc aattgcttgt ttcagaagtg ggggcataga atcggtggta
     8821 ggtgtctctc tttcctcttt tgctacttga tgctcctgtt cctccaatac gcagcccagt
     8881 gtaaagtggc ccacggcgga cagagcgtac agtgcgttct ccagggagaa gccttgctga
     8941 cacaggaacg cgagctgatt ttccagggtt tcgtactgtt tctctgttgg gcgggtgccg
     9001 agatgcactt tagccccgtc acgatgtgag aggagagcac agcggtatga cttggcgttg
     9061 ttccgcagaa agtcttgcca tgactcgcct tccagggggc aggagtgggt atgatgcctg
     9121 tccagcatct cgattggcag ggcatcgagc agggcccgct tgttcttcac gtgccagtac
     9181 agggtaggct gctcaactcc cagcttttga gcgagtttcc ttgtcgtcag gccttcgata
     9241 ccgactccat tgagtaattc cagagcagag tttatgactt tgctcttgtc cagtctagac
     9301 atcttatcgt catcgtcttt gtaatccatg gtggcggatc ccgcgtcacg acacctgtgt
     9361 tctggcggca aacccgttgc gaaaaagaac gttcacggcg actactgcac ttatatacgg
     9421 ttctccccca ccctcgggaa aaaggcggag ccagtacacg acatcacttt cccagtttac
     9481 cccgcgccac cttctctagg caccggttca attgccgacc cctcccccca acttctcggg
     9541 cactgtgggc catgtgcgct ctgccccatg gtaatagcca tgactaatac gtagatgtac
     9601 tgccaagtag gaaagtgcca taaggtcatg tactgggcat aatgccaggc gggccattta
     9661 ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag
     9721 tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtgcc tattggcgtt
     9781 actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca
     9841 ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat
     9901 gaccccgtaa ttgattacta ttaataacta gtcaataatc aatgtcataa gctctaatcg
     9961 cataatcgag ctaagagcga ttaatatgct tagctagctt agctaagcta gatttctcta
    10021 ttctcccaca atatatagca gctattagct tagcagtccg aatacgatcg tccacgatcc
    10081 ataagcgtaa cacatttgag tagcgactga cgggcaccgg agccactcga gtggaatt
//
