LOCUS       sequence-442474-        8557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposon plasmid for doxycycline inducible expression of 
            MYBL1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8557)
  AUTHORS   Pierson Smela M, Adams J, Ma C, Breimann L, Widocki U, Shioda T, 
            Church GM
  TITLE     Induction of Meiosis from Human Pluripotent Stem Cells
  JOURNAL   bioRxiv 2024.05.31.596483
REFERENCE   2  (bases 1 to 8557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.31.596483"
COMMENT     Sequence Label: pPB-cT3G-cERP2-MYBL1
FEATURES             Location/Qualifiers
     source          1..8557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    16..34
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    52..70
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    88..106
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    124..142
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    160..178
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    196..214
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    232..250
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     277..301
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    367..391
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2471..2495)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2497..2523
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    2690..2824
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   3096..3130
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(3141..3158)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3141..3157)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3150..3172)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3366..3385)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3487..3509
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3548..3566)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3634..3738
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3739..3807,3808..4599)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3957..3976)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4770..5358
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5259..5278
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5512..5529
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5735..5756
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5771..5776,5777..5794,5795..5801)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5809..5825
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5814..5836
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5833..5849
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5833..5849
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   5875..5937
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    6113..6194
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(6911..6967)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(6977..7720)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(7721..7744)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      7744..7753
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7773..7984)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        7985..8364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tcacctcgag tttactccct atcagtgata gagaacgtat gaagagttta ctccctatca
       61 gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt ataaggagtt
      121 tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt gatagagaac
      181 gtatctacag tttactccct atcagtgata gagaacgtat atccagttta ctccctatca
      241 gtgatagaga acgtataagc ttgcctatat aagcagagct cgtttagtga accgtcagat
      301 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
      361 cctaccacaa gtttgtacaa aaaagttggc accatggcga agaggtcgcg cagtgaggat
      421 gaggatgatg accttcagta tgccgatcat gattatgaag taccacaaca aaaaggactg
      481 aagaaactct ggaacagagt aaaatggaca agggacgagg atgataaatt aaagaagttg
      541 gttgaacaac atggaactga tgattggact ctaattgcta gtcatcttca aaatcgctct
      601 gattttcagt gccagcatcg atggcagaaa gttttaaatc ctgaattgat aaagggtcct
      661 tggactaaag aagaagatca gagggttatt gaattagttc agaaatatgg gccaaaaaga
      721 tggtctttaa ttgcaaaaca tttaaaagga agaataggca agcagtgtag agaaagatgg
      781 cataatcatc tgaatcctga ggtaaagaaa tcttcctgga cagaagagga ggacaggatc
      841 atctatgaag cacataagcg gttgggaaat cgttgggcag aaattgccaa actacttcca
      901 ggaaggactg ataattctat caaaaatcat tggaattcta ctatgcgaag aaaagtggaa
      961 caggagggct atttacaaga tggaataaaa tcagaacgat cttcatctaa acttcaacac
     1021 aaaccttgtg cagctatgga tcatatgcaa acccagaatc agttttacat acctgttcag
     1081 atccctgggt atcagtatgt gtcacctgaa ggcaattgta tagaacatgt tcagcctact
     1141 tctgccttta ttcagcaacc cttcattgat gaagatcctg ataaggaaaa gaaaataaag
     1201 gaacttgaga tgcttcttat gtcagctgag aatgaagtta gaagaaagcg aattccatca
     1261 cagcctggaa gtttttctag ctggtctggt agtttcctca tggatgataa catgtctaat
     1321 actctaaata gccttgacga gcacactagt gagttttaca gtatggatga aaatcagcct
     1381 gtgtctgctc agcagaattc acccacaaag ttcctggccg tggaggcaaa cgctgtgtta
     1441 tcctctttgc agaccatccc agaatttgca gagactctag aacttattga atctgatcct
     1501 gtagcatgga gtgacgttac cagttttgat atttctgatg ctgctgcttc tcctatcaaa
     1561 tccaccccag ttaaattaat gagaattcag cacaatgaag gagccatgga atgccaattt
     1621 aacgtcagtc ttgtacttga agggaaaaaa aacacttgta atggtggcaa cagtgaagct
     1681 gttcctttaa catccccaaa tatagccaag tttagcactc caccagccat cctcagaaag
     1741 aagagaaaaa tgcgagtggg tcattcccca ggcagcgaac ttagggatgg ctcattgaac
     1801 gatggtggta atatggcgct aaaacataca ccactgaaaa cactaccatt ttctccttca
     1861 cagtttttca acacatgtcc tggtaatgaa caacttaata tagaaaatcc ttcatttaca
     1921 tcaaccccta tttgtgggca gaaagctctc attacaactc ctcttcataa ggaaacaact
     1981 cccaaagatc aaaaggaaaa tgtagggttt agaacaccta ctattagaag atctatactg
     2041 ggtaccacac caagaactcc tactcctttt aagaatgcgc ttgctgctca ggagaaaaaa
     2101 tatggacctc ttaaaattgt gtcccagcca cttgctttct tggaagaaga tattcgggaa
     2161 gttttaaaag aagaaactgg aacagaccta ttcctcaaag aggaagatga acctgcttac
     2221 aaaagctgca aacaagagaa taccgcttct gggaagaaag tcagaaaatc actagtctta
     2281 gataattggg aaaaagaaga atcaggcact caactgttga ctgaagacat ttcagacatg
     2341 cagtcaaatt gtgaatggga aacagtggtt tatgggaaga cagaagacca acttattatg
     2401 actgaacaag caagaagata tctgagtact tacacagcta ccagtagtac ttcaagagct
     2461 ctcatactgt tgccaacttt cttgtacaaa gtggtatacc catacgatgt tccagattac
     2521 gcttagtaat gagaaatact cagaagatgt cactcaccat agtccgcagt gaaagataaa
     2581 tgatctcttt gtcgtatggt gtcaagtttt agagctagaa atagcaagta ggccactgag
     2641 caagaactat gaatgagatc tttagacata ttgcggccgc tttctccgcg atccagacat
     2701 gataagatac attgatgagt ttggacaaac caaaactaga atgcagtgaa aaaaatgcct
     2761 tatttgtgaa atttgtgatg ctattgcctt atttgtaacc attataagct gcaataaaca
     2821 agttatctat aacaagaaaa tatatatata ataagttatc acgtaagtag aacatgaaat
     2881 aacaatataa ttatcgtatg agttaaatct taaaagtcac gtaaaagata atcatgcgtc
     2941 attttgactc acgcggtcgt tatagttcaa aatcagtgac acttaccgca ttgacaagca
     3001 cgcctcacgg gagctccaag cggcgactga gatgtcctaa atgcacagcg acggattcgc
     3061 gctatttaga aagagagagc aatatttcaa gaatgcatgc gtcaatttta cgcagactat
     3121 ctttctaggg ttaagaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     3181 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     3241 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
     3301 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
     3361 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     3421 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     3481 atgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cacgcgagtt
     3541 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     3601 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     3661 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     3721 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     3781 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     3841 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     3901 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     3961 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     4021 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     4081 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     4141 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     4201 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     4261 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     4321 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     4381 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     4441 gccggtgagc gtggttcacg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     4501 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     4561 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     4621 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     4681 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     4741 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     4801 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     4861 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     4921 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     4981 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     5041 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     5101 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     5161 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     5221 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     5281 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     5341 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     5401 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     5461 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     5521 gtgagcgagg aagcggatga gcgcccaata cgcaaaccgc ctctcaccgc gcgttggccg
     5581 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     5641 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     5701 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     5761 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     5821 aacaatttca cacaggaaac agctatgacc atgattacgc caaggtcgac ttaaccctag
     5881 aaagataatc atattgtgac gtacgttaaa gataatcatg cgtaaaattg acgcatgtgt
     5941 tttatcggtc tgtatatcga ggtttattta ttaatttgaa tagatattaa gttttattat
     6001 atttacactt acatactaat aataaattca acaaacaatt tatttatgtt tatttattta
     6061 ttaaaaaaaa acaaaaactc aaaatttctt ctataaagta acaaaacttt tagcagtgaa
     6121 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     6181 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     6241 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
     6301 ctctggagat tcatgcgcca ggttttctgg tcatgcacca cgttcgtggg ccctcaggaa
     6361 cctccacgtc agcggtaact gtgaagccca gtcgctcgta aaaaggaaga ttccggggag
     6421 cactggtttc gaggaacgcc ggcacgccag cccgttccgc ggcctcaaca ccaggaagaa
     6481 cgacagcgct tcccaaacct ttaccttggt gatctgggct gacaccgaca gtggcaagaa
     6541 accacgcagg ctcctttggt ctatgaggag ccagcaagcc ctccatttgc tgttgtgccg
     6601 ccaacctact ccctgacagc tcggccatcc gcggacctat ttccgcgaac actgcgccgg
     6661 cttcgacgct ttccggagta gtccagaccg caactgcggc accatcgtcc gcaacccaaa
     6721 ctttaccaat gtccagaccc acgcgcgtaa gaaacagctc ttgcaactca gttaccctct
     6781 ctatgtgtct gtccggatct acggtatggc gggtcgctgg ataatctgca aatgctgcgg
     6841 caagtgttcg gacagcccgt ggtacatcgt cacgggtagc gagcctaaca gtgggcttat
     6901 attccgtcat ggggccgggg ttctcctcca cgtcgccggc ctgcttcagc aggctgaagt
     6961 tggtggcgcc gctgcccccg gggagcatgt caaggtcaaa atcgtcaaga gcgtcagcag
     7021 gcagcatatc aaggtcaaag tcgtcaaggg catcggctgg gagcatgtct aagtcaaaat
     7081 cgtcaagggc gtcggtcggc ccgccgcttt cgcactttag ctgtttctcc aggccacata
     7141 tgattagttc caggccgaaa aggaaggcag gttcggctcc ctgccggtcg aacagctcaa
     7201 ttgcttgttt cagaagtggg ggcatagaat cggtggtagg tgtctctctt tcctcttttg
     7261 ctacttgatg ctcctgttcc tccaatacgc agcccagtgt aaagtggccc acggcggaca
     7321 gagcgtacag tgcgttctcc agggagaagc cttgctgaca caggaacgcg agctgatttt
     7381 ccagggtttc gtactgtttc tctgttgggc gggtgccgag atgcacttta gccccgtcac
     7441 gatgtgagag gagagcacag cggtatgact tggcgttgtt ccgcagaaag tcttgccatg
     7501 actcgccttc cagggggcag gagtgggtat gatgcctgtc cagcatctcg attggcaggg
     7561 catcgagcag ggcccgcttg ttcttcacgt gccagtacag ggtaggctgc tcaactccca
     7621 gcttttgagc gagtttcctt gtcgtcaggc cttcgatacc gactccattg agtaattcca
     7681 gagcagagtt tatgactttg ctcttgtcca gtctagacat cttatcgtca tcgtctttgt
     7741 aatccatggt ggcggatccc gcgtcacgac acctgtgttc tggcggcaaa cccgttgcga
     7801 aaaagaacgt tcacggcgac tactgcactt atatacggtt ctcccccacc ctcgggaaaa
     7861 aggcggagcc agtacacgac atcactttcc cagtttaccc cgcgccacct tctctaggca
     7921 ccggttcaat tgccgacccc tccccccaac ttctcgggca ctgtgggcca tgtgcgctct
     7981 gccccatggt aatagccatg actaatacgt agatgtactg ccaagtagga aagtgccata
     8041 aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg
     8101 gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact
     8161 ccacccattg acgtcaatgg aaagtgccta ttggcgttac tatgggaaca tacgtcatta
     8221 ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt
     8281 atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt
     8341 aataactagt caataatcaa tgtcataagc tctaatcgca taatcgagct aagagcgatt
     8401 aatatgctta gctagcttag ctaagctaga tttctctatt ctcccacaat atatagcagc
     8461 tattagctta gcagtccgaa tacgatcgtc cacgatccat aagcgtaaca catttgagta
     8521 gcgactgacg ggcaccggag ccactcgagt ggaatta
//
