LOCUS       sequence-442475-       11875 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposon plasmid for doxycycline inducible expression of 
            TET3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11875)
  AUTHORS   Pierson Smela M, Adams J, Ma C, Breimann L, Widocki U, Shioda T, 
            Church GM
  TITLE     Induction of Meiosis from Human Pluripotent Stem Cells
  JOURNAL   bioRxiv 2024.05.31.596483
REFERENCE   2  (bases 1 to 11875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.31.596483"
COMMENT     Sequence Label: pPB-cT3G-cERP2-TET3
FEATURES             Location/Qualifiers
     source          1..11875
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    53..71
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    89..107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    197..215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    233..251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     278..302
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    368..392
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    3164..3175
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    complement(5790..5814)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5816..5842
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    6009..6143
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   6415..6449
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6460..6477)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6460..6476)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6469..6491)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6685..6704)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6806..6828
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6867..6885)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6953..7057
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7058..7126,7127..7918)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7276..7295)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8089..8677
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8578..8597
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8831..8848
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9054..9075
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9090..9095,9096..9113,9114..9120)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9128..9144
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9133..9155
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9152..9168
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9152..9168
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   9194..9256
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    9432..9513
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(10230..10286)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(10296..11039)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(11040..11063)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      11063..11072
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(11092..11303)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        11304..11683
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
       61 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
      121 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
      181 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
      241 agtgatagag aacgtataag cttgcctata taagcagagc tcgtttagtg aaccgtcaga
      301 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      361 gcctaccaca agtttgtaca aaaaagcagg cttagccacc atgagccagt ttcaggtgcc
      421 cctggccgtc cagccggacc tgccaggcct ttatgacttc cctcagcgcc aggtgatggt
      481 agggagcttc ccggggtctg ggctctccat ggctgggagt gagtcccaac tccgaggggg
      541 tggagatggt cgaaagaaac ggaaacggtg tggtacttgt gagccctgcc ggcggctgga
      601 aaactgtggc gcttgcacta gctgtaccaa ccgccgcacg caccagatct gcaaactgcg
      661 aaaatgtgag gtgctgaaga aaaaagtagg gcttctcaag gaggtggaaa taaaggctgg
      721 tgaaggagcc gggccgtggg gacaaggagc ggctgtcaag acaggctcag agctcagccc
      781 agttgatgga cctgttccag gtcagatgga ctcagggcca gtgtaccatg gggactcacg
      841 gcagctaagc gcctcagggg tgccggtcaa tggtgctaga gagcccgctg gacccagtct
      901 gctggggact gggggtcctt ggcgggtaga ccaaaagccc gactgggagg ctgccccagg
      961 cccagctcat actgctcgcc tggaagatgc ccacgatctg gtggcctttt cggctgtggc
     1021 cgaagctgtg tcctcttatg gggcccttag cacccggctc tatgaaacct tcaaccgtga
     1081 gatgagtcgt gaggctggga acaacagcag gggaccccgg ccagggcctg agggctgctc
     1141 tgctggcagc gaagaccttg acacactgca gacggccctg gccctcgcgc ggcatggtat
     1201 gaaaccaccc aactgcaact gcgatggccc agaatgccct gactacctcg agtggctgga
     1261 ggggaagatc aagtctgtgg tcatggaagg aggggaggag cggcccaggc tcccagggcc
     1321 tctgcctcct ggtgaggccg gcctcccagc accaagcacc aggccactcc tcagctcaga
     1381 ggtgccccag atctctcccc aagagggcct gcccctgtcc cagagtgccc tgagcattgc
     1441 caaggaaaaa aacatcagct tgcagaccgc cattgccatt gaggccctca cacagctctc
     1501 ctctgccctc ccgcagcctt ctcattccac cccccaggct tcttgccccc ttcctgaggc
     1561 cttgtcacct cctgcccctt tcagatctcc ccagtcttac ctccgggctc cctcatggcc
     1621 tgtggttcct cctgaagagc actcatcttt tgctcctgat agctctgcct tccctccagc
     1681 aactcctaga actgagttcc ctgaagcctg gggcactgac acccctccag caacgccccg
     1741 gagctcctgg cccatgcctc gcccaagccc cgatcccatg gctgaactgg agcagttgtt
     1801 gggcagcgcc agtgattaca tccagtcagt attcaagcgg cctgaggccc tgcctaccaa
     1861 gcccaaggtc aaggtggagg caccctcttc ctccccggcc ccggccccat cccctgtact
     1921 tcagagggag gctcccacgc catcctcgga gcccgacacc caccagaagg cccagaccgc
     1981 cctgcagcag cacctccacc acaagcgcag cctcttccta gaacaggtgc acgacacctc
     2041 cttccctgct ccttcagagc cttctgctcc tggctggtgg cccccaccaa gttcacctgt
     2101 cccacggctt ccagacagac cacccaagga gaagaagaag aagctcccaa caccagctgg
     2161 aggtcccgtg ggaacggaga aagctgcccc tgggatcaag cccagtgtcc gaaagcccat
     2221 tcagatcaag aagtccaggc cccgggaagc acagcccctc ttcccacctg tccgacagat
     2281 tgtcctggaa gggcttaggt ccccagcctc ccaggaagtg caggctcatc caccggcccc
     2341 tctgcctgcc tcacagggct ctgctgtgcc cctgccccca gaaccttctc ttgcgctatt
     2401 tgcacctagt ccctccaggg acagcctgct gccccctact caggaaatga ggtcccccag
     2461 ccccatgaca gccttgcagc caggctccac tggccctctt ccccctgccg atgacaagct
     2521 ggaagagctc atccggcagt ttgaggctga atttggagat agctttgggc ttcccggccc
     2581 cccttctgtg cccattcagg accccgagaa ccagcaaaca tgtctcccag cccctgagag
     2641 cccctttgct acccgttccc ccaagcaaat caagattgag tcttcggggg ctgtgactgt
     2701 gctctcaacc acctgcttcc attcagagga gggaggacag gaggccacac ccaccaaggc
     2761 tgagaaccca ctcacaccca ccctcagtgg cttcttggag tcacctctta agtacctgga
     2821 cacacccacc aagagtctgc tggacacacc tgccaagaga gcccaggccg agttccccac
     2881 ctgcgattgc gtcgaacaaa tagtggagaa agatgaaggt ccatattata ctcacttggg
     2941 atctggcccc acggtcgcct ctatccggga actcatggag gagcggtatg gagagaaggg
     3001 gaaagccatc cggatcgaga aggtcatcta cacggggaag gagggaaaga gctcccgcgg
     3061 ttgccccatt gcaaagtggg tgatccgcag gcacacgctg gaggagaagc tactctgcct
     3121 ggtgcggcac cgggcaggcc accactgcca gaacgctgtg atcgtcatcc tcatcctggc
     3181 ctgggagggc attccccgta gcctcggaga caccctctac caggagctca ccgacaccct
     3241 ccggaagtat gggaacccca ccagccggag atgcggcctc aacgatgacc ggacctgcgc
     3301 ttgccaaggc aaagacccca acacctgtgg tgcctccttc tcctttggtt gttcctggag
     3361 catgtacttc aacggctgca agtatgctcg gagcaagaca cctcgcaagt tccgcctcgc
     3421 aggggacaat cccaaagagg aagaagtgct ccggaagagt ttccaggacc tggccaccga
     3481 agtcgctccc ctgtacaagc gactggcccc tcaggcctat cagaaccagg tgaccaacga
     3541 ggaaatagcg attgactgcc gtctggggct gaaggaagga cggcccttcg cgggggtcac
     3601 ggcctgcatg gacttctgtg cccacgccca caaggaccag cataacctct acaatgggtg
     3661 caccgtggtc tgcaccctga ccaaggaaga caatcgctgc gtgggcaaga ttcccgagga
     3721 tgagcagctg catgttctcc ccctgtacaa gatggccaac acggatgagt ttggtagcga
     3781 ggagaaccag aatgcaaagg tgggcagcgg agccatccag gtgctcaccg ccttcccccg
     3841 cgaggtccga cgcctgcccg agcctgccaa gtcctgccgc cagcggcagc tggaagccag
     3901 aaaggcagca gccgagaaga agaagattca gaaggagaag ctgagcactc cggagaagat
     3961 caagcaggag gccctggagc tggcgggcat tacgtcggac ccaggcctgt ctctgaaggg
     4021 tggattgtcc cagcaaggcc tgaagccctc cctcaaggtg gagccgcaga accacttcag
     4081 ctccttcaag tacagcggca acgcggtggt ggagagctac tcggtgctgg gcaactgccg
     4141 gccctccgac ccttacagca tgaacagcgt gtactcctac cactcctact atgcacagcc
     4201 cagcctgacc tccgtcaatg gcttccactc caagtacgct ctcccgtctt ttagctacta
     4261 tggctttcca tccagcaacc ccgtcttccc ctctcagttc ctgggtcctg gtgcctgggg
     4321 gcatagtggc agcagtggca gttttgagaa gaagccagac ctccacgctc tgcacaacag
     4381 cctgagcccg gcctacggtg gtgctgagtt tgccgagctg cccagccagg ctgttcccac
     4441 agacgcccac caccccactc ctcaccacca gcagcctgcg tacccaggcc ccaaggagta
     4501 tctgcttccc aaggcccccc tactccactc agtgtccagg gacccctccc cctttgccca
     4561 gagctccaac tgctacaaca gatccatcaa gcaagagcca gtagacccgc tgacccaggc
     4621 tgagcctgtg cccagagacg ctggcaagat gggcaagaca cctctgtccg aggtgtctca
     4681 gaatggagga cccagtcacc tttggggaca gtactcagga ggcccaagca tgtcccccaa
     4741 gaggactaac ggtgtgggtg gcagctgggg tgtgttctcg tctggggaga gtcctgccat
     4801 cgtccctgac aagctcagtt cctttggggc cagctgcctg gccccttccc acttcacaga
     4861 tggccagtgg gggctgttcc ccggtgaggg gcagcaggca gcttcccact ctggaggacg
     4921 gctgcgaggc aaaccgtgga gcccctgcaa gtttgggaac agcacctcgg ccttggctgg
     4981 gcccagcctg actgagaagc cgtgggcgct gggggcaggg gatttcaact cggccctgaa
     5041 aggtagtcct gggttccaag acaagctgtg gaaccccatg aaaggagagg agggcaggat
     5101 tccagccgca ggggccagcc agctggacag ggcctggcag tcctttggtc tgcccctggg
     5161 atccagcgag aagctgtttg gggctctgaa gtcagaggag aagctgtggg accccttcag
     5221 cctggaggag gggccggctg aggagccccc cagcaaggga gcggtgaagg aggagaaggg
     5281 cggtggtggt gcggaggagg aagaggagga gctgtggtcg gacagtgaac acaacttcct
     5341 ggacgagaac atcggcggcg tggccgtggc cccagcccac ggctccatcc tcatcgagtg
     5401 tgcccggcgg gagctgcacg ccaccacgcc gcttaagaag cccaaccgct gccaccccac
     5461 ccgcatctcg ctggtcttct accagcacaa gaacctcaac cagcccaacc acgggctggc
     5521 cctctgggaa gccaagatga agcagctggc ggagagggca cgggcacggc aggaggaggc
     5581 tgcccggctg ggcctgggcc agcaggaggc caagctctac gggaagaagc gcaagtgggg
     5641 gggcactgtg gttgctgagc cccagcagaa agagaagaag ggggtcgtcc ccacccggca
     5701 ggcactggct gtgcccacag actcggcggt caccgtgtcc tcctatgcct acacgaaggt
     5761 cactggcccc tacagccgct ggatctagta cccagctttc ttgtacaaag tggtataccc
     5821 atacgatgtt ccagattacg cttagtaatg agaaatactc agaagatgtc actcaccata
     5881 gtccgcagtg aaagataaat gatcagatct ctagaaatct aaaagtttta gagctagaaa
     5941 tagcaagtag gccactgagc aagaactatg aatgagatct ttagacatat tgcggccgct
     6001 ttctccgcga tccagacatg ataagataca ttgatgagtt tggacaaacc aaaactagaa
     6061 tgcagtgaaa aaaatgcctt atttgtgaaa tttgtgatgc tattgcctta tttgtaacca
     6121 ttataagctg caataaacaa gttatctata acaagaaaat atatatataa taagttatca
     6181 cgtaagtaga acatgaaata acaatataat tatcgtatga gttaaatctt aaaagtcacg
     6241 taaaagataa tcatgcgtca ttttgactca cgcggtcgtt atagttcaaa atcagtgaca
     6301 cttaccgcat tgacaagcac gcctcacggg agctccaagc ggcgactgag atgtcctaaa
     6361 tgcacagcga cggattcgcg ctatttagaa agagagagca atatttcaag aatgcatgcg
     6421 tcaattttac gcagactatc tttctagggt taagaattca ctggccgtcg ttttacaacg
     6481 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     6541 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     6601 cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     6661 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     6721 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     6781 ttacagacaa gctgtgacca tgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     6841 tcaccgaaac acgcgagttc gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     6901 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     6961 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     7021 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     7081 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     7141 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     7201 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     7261 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     7321 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     7381 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     7441 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     7501 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     7561 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     7621 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     7681 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     7741 tattgctgat aaatctggag ccggtgagcg tggttcacgc ggtatcattg cagcactggg
     7801 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     7861 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     7921 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     7981 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     8041 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8101 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8161 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8221 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8281 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8341 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     8401 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     8461 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     8521 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     8581 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     8641 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     8701 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     8761 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     8821 gaccgagcgc agcgagtcag tgagcgagga agcggatgag cgcccaatac gcaaaccgcc
     8881 tctcaccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     8941 agcgggcagt gagcgcaacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     9001 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     9061 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     9121 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     9181 aaggtcgact taaccctaga aagataatca tattgtgacg tacgttaaag ataatcatgc
     9241 gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag gtttatttat taatttgaat
     9301 agatattaag ttttattata tttacactta catactaata ataaattcaa caaacaattt
     9361 atttatgttt atttatttat taaaaaaaaa caaaaactca aaatttcttc tataaagtaa
     9421 caaaactttt agcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     9481 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     9541 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     9601 ggtatggctg attatgatcc tctggagatt catgcgccag gttttctggt catgcaccac
     9661 gttcgtgggc cctcaggaac ctccacgtca gcggtaactg tgaagcccag tcgctcgtaa
     9721 aaaggaagat tccggggagc actggtttcg aggaacgccg gcacgccagc ccgttccgcg
     9781 gcctcaacac caggaagaac gacagcgctt cccaaacctt taccttggtg atctgggctg
     9841 acaccgacag tggcaagaaa ccacgcaggc tcctttggtc tatgaggagc cagcaagccc
     9901 tccatttgct gttgtgccgc caacctactc cctgacagct cggccatccg cggacctatt
     9961 tccgcgaaca ctgcgccggc ttcgacgctt tccggagtag tccagaccgc aactgcggca
    10021 ccatcgtccg caacccaaac tttaccaatg tccagaccca cgcgcgtaag aaacagctct
    10081 tgcaactcag ttaccctctc tatgtgtctg tccggatcta cggtatggcg ggtcgctgga
    10141 taatctgcaa atgctgcggc aagtgttcgg acagcccgtg gtacatcgtc acgggtagcg
    10201 agcctaacag tgggcttata ttccgtcatg gggccggggt tctcctccac gtcgccggcc
    10261 tgcttcagca ggctgaagtt ggtggcgccg ctgcccccgg ggagcatgtc aaggtcaaaa
    10321 tcgtcaagag cgtcagcagg cagcatatca aggtcaaagt cgtcaagggc atcggctggg
    10381 agcatgtcta agtcaaaatc gtcaagggcg tcggtcggcc cgccgctttc gcactttagc
    10441 tgtttctcca ggccacatat gattagttcc aggccgaaaa ggaaggcagg ttcggctccc
    10501 tgccggtcga acagctcaat tgcttgtttc agaagtgggg gcatagaatc ggtggtaggt
    10561 gtctctcttt cctcttttgc tacttgatgc tcctgttcct ccaatacgca gcccagtgta
    10621 aagtggccca cggcggacag agcgtacagt gcgttctcca gggagaagcc ttgctgacac
    10681 aggaacgcga gctgattttc cagggtttcg tactgtttct ctgttgggcg ggtgccgaga
    10741 tgcactttag ccccgtcacg atgtgagagg agagcacagc ggtatgactt ggcgttgttc
    10801 cgcagaaagt cttgccatga ctcgccttcc agggggcagg agtgggtatg atgcctgtcc
    10861 agcatctcga ttggcagggc atcgagcagg gcccgcttgt tcttcacgtg ccagtacagg
    10921 gtaggctgct caactcccag cttttgagcg agtttccttg tcgtcaggcc ttcgataccg
    10981 actccattga gtaattccag agcagagttt atgactttgc tcttgtccag tctagacatc
    11041 ttatcgtcat cgtctttgta atccatggtg gcggatcccg cgtcacgaca cctgtgttct
    11101 ggcggcaaac ccgttgcgaa aaagaacgtt cacggcgact actgcactta tatacggttc
    11161 tcccccaccc tcgggaaaaa ggcggagcca gtacacgaca tcactttccc agtttacccc
    11221 gcgccacctt ctctaggcac cggttcaatt gccgacccct ccccccaact tctcgggcac
    11281 tgtgggccat gtgcgctctg ccccatggta atagccatga ctaatacgta gatgtactgc
    11341 caagtaggaa agtgccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg
    11401 tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg
    11461 gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtgcctat tggcgttact
    11521 atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc
    11581 gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac
    11641 cccgtaattg attactatta ataactagtc aataatcaat gtcataagct ctaatcgcat
    11701 aatcgagcta agagcgatta atatgcttag ctagcttagc taagctagat ttctctattc
    11761 tcccacaata tatagcagct attagcttag cagtccgaat acgatcgtcc acgatccata
    11821 agcgtaacac atttgagtag cgactgacgg gcaccggagc cactcgagtg gaatt
//
