LOCUS       sequence-442476-        9209 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zebrafish -2.1prox1a_1 (mouse conserved) enhancer reporter in the 
            lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal 
            and cardiac muscle. Shows the high background typical of the ZED 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9209)
  AUTHORS   Panara V, Yu H, Peng D, Staxang K, Hodik M, Filipek-Gorniok B, 
            Kazenwadel J, Skoczylas R, Mason E, Allalou A, Harvey NL, Haitina T,
            Hogan BM, Koltowska K
  TITLE     Multiple cis-regulatory elements control prox1a expression in 
            distinct lymphatic vascular beds.
  JOURNAL   Development. 2024 May 1;151(9):dev202525. doi: 10.1242/dev.202525. 
            Epub 2024 May 9.
  PUBMED    38722096
REFERENCE   2  (bases 1 to 9209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2024-05-1"; volume: "151(9):dev202525. doi"; 
            pages: " 10.1242/dev.202525. Epub 2024 May 9"
COMMENT     Sequence Label: Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2
FEATURES             Location/Qualifiers
     source          1..9209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(15..26,27..214)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     protein_bind    complement(301..334)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      1012..1021
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1018..1692
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(1198..1218)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     1610..1633
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    1817..1938
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1999..2015)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2610..2630)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2610..2628)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(2633..2666)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    2687..2808
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(3018..3731,3732..3734,3735..3737))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(3021..3731,3732..3734,3735..3737))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3063..3084)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3412..3431
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3671..3692
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      3734..3743
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4801..4817)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4820..4853)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4862..4886
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5093..5117)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5709..5742)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    join(6109..6271,6272..6283)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     promoter        complement(6334..6352)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6335..6352)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6370..6386)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6370..6386)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6383..6405)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6394..6410
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6418..6424,6425..6442,6443..6448))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6463..6484
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6601..6618)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6772..7360)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6852..6871)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7531..8322,8323..8391))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8154..8173
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8392..8496)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8574..9029)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8711..8732)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8923..8942
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9155..9177
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9169..9186
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9170..9186
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(9193..9209,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(9193..9209,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccagagg tgtaaagtac ttgagtaatt ttacttgatt actgtactta
       61 agtattattt ttggggattt ttactttact tgagtacaat taaaaatcaa tacttttact
      121 tttacttaat tacatttttt tagaaaaaaa agtacttttt actccttaca attttattta
      181 cagtcaaaaa gtacttattt tttggagatc acttcattct attttccctt gctattacca
      241 aaccaattga attgcgctga tgcccagttt aatttaaata gatctgtcga cttaattaat
      301 gaagttccta tactttctag agaataggaa cttcctcact atagggcgaa ttggagctcc
      361 accgcggtgg cggccgctct agaattctgc acattggctg tttcccaaca gagtgcaagc
      421 tctgagagca aatcttgccc gtcattactg tttgatataa ggtattccca tgcctgacgc
      481 acataatatt tttgttacat tcgtgtttac agcactgaag gcaggcagct atttgtgcag
      541 tcagtacttt atgtatttct aaacccagtg ctggagaggc caataggcag gatgcccccc
      601 ccgcgttagt ccccctgcac aattgtgctg cacctgtcta ctccatttgc agacccctgt
      661 gtctgtgcaa actatttctt tcattgtgct gttttttttg tcacccagca ttacagacat
      721 gcttttttgg gaatccctat ttggccatcc ctagtagtgc tccccccacc tccatacttt
      781 ccatacatgg gctaaggggt ccaaagaccc tgccctcccc cctcacctac tccattaatg
      841 gcttctttgc ttttcaatgg cccagaagct accaaataag ggcaggctgc ctgcctttcc
      901 ctgcggagct cccactgact cctcaactcc aggcagcgta taaattgaca gctcagtccc
      961 caggcagcac ccagccaatt caatcacagc acggggatcg atccaccggt cgccaccatg
     1021 gcctcctccg agaacgtcat caccgagttc atgcgcttca aggtgcgcat ggagggcacc
     1081 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggccac
     1141 aacaccgtga agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
     1201 tccccccagt tccagtacgg ctccaaggtg tacgtgaagc accccgccga catccccgac
     1261 tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     1321 ggcggcgtgg cgaccgtgac ccaggactcc tccctgcagg acggctgctt catctacaag
     1381 gtgaagttca tcggcgtgaa cttcccctcc gacggccccg tgatgcagaa gaagaccatg
     1441 ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagacc
     1501 cacaaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa gtccatctac
     1561 atggccaaga agcccgtgca gctgcccggc tactactacg tggacgccaa gctggacatc
     1621 acctcccaca acgaggacta caccatcgtg gagcagtacg agcgcaccga gggccgccac
     1681 cacctgttcc tgagatcggc cgcgactcta gatcataatc agccatacca catttgtaga
     1741 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     1801 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     1861 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     1921 actcatcaat gtatcttaag atcctagaac tagaactagt ggatcccccg ggctgcagga
     1981 attcgatatc aagcttatcg ataccgtcga cctcgagcgg ccgccagtgt gatggatatc
     2041 tgcagaattc ggcttacaca ctcaagctgg ttctttgaac agagtttaag aaagaagcta
     2101 tttacaatgt tgggggtgga atttaagaag aaaggacagt ggataatgaa aaggatactc
     2161 agaacaactg ctggaacaca gacatcactg tcttgacaaa gtcagctggc aggaactatg
     2221 accttggaga aggatataag caactttctt gttatgtggc aaagaggaag ttgcagacct
     2281 ttgacttcac tctccttctg cctctaaaag ccgaattcca gcacactggc ggccgttact
     2341 agtggatccc ccgggctgca ggaattcgat tatgtcaaaa acctgtactt cataaaattg
     2401 gaaatgacaa ttttcttgat aggtatcact taccaaagtt aaatgaaaat catattaaca
     2461 atttaaatag acctgtaata tataagaaaa taaaagcaat cattaaaaga ctccaaatcc
     2521 cccccggccc cccaaagaaa gcccaaagac tgttttagga cagaattcct gcagcccggg
     2581 ggatccgagc tcggtaccag cttttgttcc ctttagtgag ggttaattgc gtgaagttcc
     2641 tatactttct agagaatagg aacttcttaa ttaaccatgg cgtacgtaag atacattgat
     2701 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     2761 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     2821 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     2881 aacctctaca aatgtggtat ggctgattat gatcagttat ctagatccgg tactagtagg
     2941 cctattattt ttgacaccag acaagttggt aatggtagcg accggcgctc agttggaatt
     3001 ctagagtcgc ggccgcttta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg
     3061 gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg
     3121 gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg
     3181 ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg
     3241 aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg
     3301 tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc
     3361 agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag
     3421 ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac
     3481 ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag
     3541 gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac
     3601 ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg
     3661 tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc
     3721 tcgcccttgc tcaccatggt ggcgaccggt ggatcctcaa gtgtccgcgc ttagaaaatg
     3781 cttggaaaag tttacttgac actacagacc cgaaagcacc actatagtcc gttagattat
     3841 taaaatatct gtcgttagct taatgcttca ggtggcgatg ctctgtttac tttctgagca
     3901 tgcttttatt tttgaaggag tgtagaggtc ctgcttcttt gcattcaagg gtggccaggg
     3961 acacctgtaa gagaataaca gatagaagca tctcagtgtt gacgttaaac acagtgcaag
     4021 acagatctta attcacacga cgacgcctca gttaatataa gtaggctaac ttttgtagtc
     4081 aatcaattaa atcgctcaat taaatgctcg caatttcaaa taactatcgg gaagtactca
     4141 cggcgcaaag ttgcatttca gcactccaat cctgcgttta tttttcccgt gttagtcctg
     4201 cgcaaagaat gttaatcctc aagatgcctg ttgacgttcg taattcgcca ctggtttaaa
     4261 ttgtccacag cccgattgtt aaagtctccc taaaactctg cgtctgtcta aagcggagag
     4321 aagtctcatt aatgaattgt gcctgtgtac tgtcggaact ggcgccagta gattccatat
     4381 cttacgaaca tgggcgggga cacgagctgg cagtgagcgc cctgggaacc aatagcagag
     4441 cgcgctgact gccattggct cggacagcat gctttccgaa atgccacggc gactgtcggc
     4501 ctactgctcc acatccagag agctgagaga ctcatcattc agcgcagcaa tttattgaga
     4561 tctttttacc ctttttagag cttcacgttg atggtgtgta atgatgtctt tttactcaag
     4621 tgctaacaga aaagcctaaa atctaagttg cagcctaatg ctatttttta aatttatttt
     4681 taggacatta aatgatgtga ataatataat tatagacttg caggattata tttaattttt
     4741 aattgtgcag aaatgtatta aaatgcctct attctattaa tgaatatcgc gcaagcttat
     4801 cgataccgtc gacctcgaga taacttcgta tagcatacat tatacgaagt tatcataatc
     4861 aacaagtttg tacaaaaaag caggctgcag gatttttgca gcagtgaatt ttttttccaa
     4921 aacaggtgtt ccaacttatt ttgttagcat ctttccccaa aggcacgctc ggagtgcgag
     4981 cttagtggaa aatacatgac aacattaaag cagtggtgtt tggagaagga aaacgcctct
     5041 taggtgtaga ggaaacctgt ttattaacac cgccacctct gatctctgtc acacccagct
     5101 ttcttgtaca aagtggttga ttatggagtt ggcgcgcctg tcattctaaa tctctctttc
     5161 agcctaaagc tttttccccg tatcccccca ggtgtctgca ggctcaaaga gcagcgagaa
     5221 gcgttcagag gaaagcgatc ccgtgccacc ttccccgtgc ccgggctgtc cccgcacgct
     5281 gccggctcgg ggatgcgggg ggagcgccgg accggagcgg agccccgggc ggctcgctgc
     5341 tgccccctag cgggggaggg acgtaattac atccctgggg gctttgggag ggggctgtcc
     5401 ccgtgagctc ccaggcgcgc ctgtcattct aaatctctct ttcagcctaa agctttttcc
     5461 ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga gaagcgttca gaggaaagcg
     5521 atcccgtgcc accttccccg tgcccgggct gtccccgcac gctgccggct cggggatgcg
     5581 gggggagcgc cggaccggag cggagccccg ggcggctcgc tgctgccccc tagcggggga
     5641 gggacgtaat tacatccctg ggggctttgg gggggggctg tccccgtgag ctcccaggcg
     5701 cgcaactcat aacttcgtat agcatacatt atacgaagtt atggcccatc tggcctgtgt
     5761 ttcagacacc agggagtctc tgctcacgtt tcctgctatt tgcagcctct ctatcaagac
     5821 taatacacct cttcccgcat cggctgcctg tgagaggctt ttcagcactg caggattgct
     5881 tttcagcccc aaaagagcta ggcttgacac taacaatttt gagaatcagc ttctactgaa
     5941 gttaaatctg aggttttaca actttgagta gcgtgtactg gcattagatt gtctgtctta
     6001 tagtttgata attaaataca aacagttcta aagcaggata aaaccttgta tgcatttcat
     6061 ttaatgtttt ttgagattaa aagcttaaac aagaatctct agttttcttt cttgctttta
     6121 cttttacttc cttaatactc aagtacaatt ttaatggagt acttttttac ttttactcaa
     6181 gtaagattct agccagatac ttttactttt aattgagtaa aattttccct aagtacttgt
     6241 actttcactt gagtaaaatt tttgagtact ttttacacct ctgctcgacc atatgggaga
     6301 gctcccaacg cgttggatgc atagcttgag tattctatag tgtcacctaa atagcttggc
     6361 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     6421 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     6481 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     6541 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     6601 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6661 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     6721 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6781 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6841 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6901 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6961 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7021 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7081 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7141 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7201 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7261 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7321 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7381 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7441 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     7501 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     7561 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     7621 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     7681 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     7741 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     7801 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     7861 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     7921 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7981 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     8041 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     8101 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     8161 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     8221 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     8281 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     8341 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     8401 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     8461 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     8521 tgatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt
     8581 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     8641 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     8701 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     8761 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     8821 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     8881 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     8941 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     9001 cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg caactgttgg
     9061 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     9121 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     9181 gccagtgaat tgtaatacga ctcactata
//
