LOCUS       sequence-442484-       11911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express human BCR-ABL1-P190 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11911)
  TITLE     Single-base tiled screen unveils design principles of PspCas13b for 
            potent and off-target-free RNA silencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: MSCV-BCR-ABL1-p190-IRES-EGFP
FEATURES             Location/Qualifiers
     source          1..11911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    581..922
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             989..1405
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1335..1357
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1373..1389
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    join(6921..7495,7496..7498,7499..7507)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7088..7105)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7315..7334
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7508..7510,7511..7513,7514..8227)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7508..7510,7511..7513,7514..8224)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7553..7574)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7814..7833)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8161..8182
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             8306..8820
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    8982..8998
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9006..9012,9013..9030,9031..9036))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9051..9072
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9189..9206)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9360..9948)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9440..9459)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10119..10910,10911..10979))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10742..10761
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10980..11084)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11152..11170
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11208..11230)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11330..11349
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11543..11565
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11693..11712
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
      781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
      841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
      901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
      961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1381 agccctcact ccttctctag gcgccggaat tccccggcgc ccggggccgg gcctggcgag
     1441 gccgccgcgc cgccgctgag acgggccccg cgcgcagccc ggcggcgcag gtaaggccgg
     1501 ccgcgccatg gtggacccgg tgggcttcgc ggaggcgtgg aaggcgcagt tcccggactc
     1561 agagcccccg cgcatggagc tgcgctcagt gggcgacatc gagcaggagc tggagcgctg
     1621 caaggcctcc attcggcgcc tggagcagga ggtgaaccag gagcgcttcc gcatgatcta
     1681 cctgcagacg ttgctggcca aggaaaagaa gagctatgac cggcagcgat ggggcttccg
     1741 gcgcgcggcg caggcccccg acggcgcctc cgagccccga gcgtccgcgt cgcgcccgca
     1801 gccagcgccc gccgacggag ccgacccgcc gcccgccgag gagcccgagg cccggcccga
     1861 cggcgagggt tctccgggta aggccaggcc cgggaccgcc cgcaggcccg gggcagccgc
     1921 gtcgggggaa cgggacgacc ggggaccccc cgccagcgtg gcggcgctca ggtccaactt
     1981 cgagcggatc cgcaagggcc atggccagcc cggggcggac gccgagaagc ccttctacgt
     2041 gaacgtcgag tttcaccacg agcgcggcct ggtgaaggtc aacgacaaag aggtgtcgga
     2101 ccgcatcagc tccctgggca gccaggccat gcagatggag cgcaaaaagt cccagcacgg
     2161 cgcgggctcg agcgtggggg atgcatccag gcccccttac cggggacgct cctcggagag
     2221 cagctgcggc gtcgacggcg actacgagga cgccgagttg aacccccgct tcctgaagga
     2281 caacctgatc gacgccaatg gcggtagcag gcccccttgg ccgcccctgg agtaccagcc
     2341 ctaccagagc atctacgtcg ggggcatgat ggaaggggag ggcaagggcc cgctcctgcg
     2401 cagccagagc acctctgagc aggagaagcg ccttacctgg ccccgcaggt cctactcccc
     2461 ccggagtttt gaggattgcg gaggcggcta taccccggac tgcagctcca atgagaacct
     2521 cacctccagc gaggaggact tctcctctgg ccagtccagc cgcgtgtccc caagccccac
     2581 cacctaccgc atgttccggg acaaaagccg ctctccctcg cagaactcgc aacagtcctt
     2641 cgacagcagc agtcccccca cgccgcagtg ccataagcgg caccggcact gcccggttgt
     2701 cgtgtccgag gccaccatcg tgggcgtccg caagaccggg cagatctggc ccaacgatgg
     2761 cgagggcgcc ttccatggag acgcagaagc ccttcagcgg ccagtagcat ctgactttga
     2821 gcctcagggt ctgagtgaag ccgctcgttg gaactccaag gaaaaccttc tcgctggacc
     2881 cagtgaaaat gaccccaacc ttttcgttgc actgtatgat tttgtggcca gtggagataa
     2941 cactctaagc ataactaaag gtgaaaagct ccgggtctta ggctataatc acaatgggga
     3001 atggtgtgaa gcccaaacca aaaatggcca aggctgggtc ccaagcaact acatcacgcc
     3061 agtcaacagt ctggagaaac actcctggta ccatgggcct gtgtcccgca atgccgctga
     3121 gtatctgctg agcagcggga tcaatggcag cttcttggtg cgtgagagtg agagcagtcc
     3181 tggccagagg tccatctcgc tgagatacga agggagggtg taccattaca ggatcaacac
     3241 tgcttctgat ggcaagctct acgtctcctc cgagagccgc ttcaacaccc tggccgagtt
     3301 ggttcatcat cattcaacgg tggccgacgg gctcatcacc acgctccatt atccagcccc
     3361 aaagcgcaac aagcccactg tctatggtgt gtcccccaac tacgacaagt gggagatgga
     3421 acgcacggac atcaccatga agcacaagct gggcgggggc cagtacgggg aggtgtacga
     3481 gggcgtgtgg aagaaataca gcctgacggt ggccgtgaag accttgaagg aggacaccat
     3541 ggaggtggaa gagttcttga aagaagctgc agtcatgaaa gagatcaaac accctaacct
     3601 ggtgcagctc cttggggtct gcacccggga gcccccgttc tatatcatca ctgagttcat
     3661 gacctacggg aacctcctgg actacctgag ggagtgcaac cggcaggagg tgaacgccgt
     3721 ggtgctgctg tacatggcca ctcagatctc gtcagccatg gagtacctgg agaagaaaaa
     3781 cttcatccac agagatcttg ctgcccgaaa ctgcctggta ggggagaacc acttggtgaa
     3841 ggtagctgat tttggcctga gcaggttgat gacaggggac acctacacag cccatgctgg
     3901 agccaagttc cccatcaaat ggactgcacc cgagagcctg gcctacaaca agttctccat
     3961 caagtccgac gtctgggcat ttggagtatt gctttgggaa attgctacct atggcatgtc
     4021 cccttacccg ggaattgacc tgtcccaggt gtatgagctg ctagagaagg actaccgcat
     4081 ggagcgccca gaaggctgcc cagagaaggt ctatgaactc atgcgagcat gttggcagtg
     4141 gaatccctct gaccggccct cctttgctga aatccaccaa gcctttgaaa caatgttcca
     4201 ggaatccagt atctcagacg aagtggaaaa ggagctgggg aaacaaggcg tccgtggggc
     4261 tgtgagtacc ttgctgcagg ccccagagct gcccaccaag acgaggacct ccaggagagc
     4321 tgcagagcac agagacacca ctgacgtgcc tgagatgcct cactccaagg gccagggaga
     4381 gagcgatcct ctggaccatg agcctgccgt gtctccattg ctccctcgaa aagagcgagg
     4441 tcccccggag ggcggcctga atgaagatga gcgccttctc cccaaagaca aaaagaccaa
     4501 cttgttcagc gccttgatca agaagaagaa gaagacagcc ccaacccctc ccaaacgcag
     4561 cagctccttc cgggagatgg acggccagcc ggagcgcaga ggggccggcg aggaagaggg
     4621 ccgagacatc agcaacgggg cactggcttt cacccccttg gacacagctg acccagccaa
     4681 gtccccaaag cccagcaatg gggctggggt ccccaatgga gccctccggg agtccggggg
     4741 ctcaggcttc cggtctcccc acctgtggaa gaagtccagc acgctgacca gcagccgcct
     4801 agccaccggc gaggaggagg gcggtggcag ctccagcaag cgcttcctgc gctcttgctc
     4861 cgcctcctgc gttccccatg gggccaagga cacggagtgg aggtcagtca cgctgcctcg
     4921 ggacttgcag tccacgggaa gacagtttga ctcgtccaca tttggagggc acaaaagtga
     4981 gaagccggct ctgcctcgga agagggcagg ggagaacagg tctgaccagg tgacccgagg
     5041 cacagtaacg cctcccccca ggctggtgaa aaagaatgag gaagctgctg atgaggtctt
     5101 caaagacatc atggagtcca gcccgggctc cagcccgccc aacctgactc caaaacccct
     5161 ccggcggcag gtcaccgtgg cccctgcctc gggcctcccc cacaaggaag aagctggaaa
     5221 gggcagtgcc ttagggaccc ctgctgcagc tgagccagtg acccccacca gcaaagcagg
     5281 ctcaggtgca ccagggggca ccagcaaggg ccccgccgag gagtccagag tgaggaggca
     5341 caagcactcc tctgagtcgc cagggaggga caaggggaaa ttgtccaggc tcaaacctgc
     5401 cccgccgccc ccaccagcag cctctgcagg gaaggctgga ggaaagccct cgcagagccc
     5461 gagccaggag gcggccgggg aggcagtcct gggcgcaaag acaaaagcca cgagtctggt
     5521 tgatgctgtg aacagtgacg ctgccaagcc cagccagccg ggagagggcc tcaaaaagcc
     5581 cgtgctcccg gccactccaa agccacagtc cgccaagccg tcggggaccc ccatcagccc
     5641 agcccccgtt ccctccacgt tgccatcagc atcctcggcc ctggcagggg accagccgtc
     5701 ttccaccgcc ttcatccctc tcatatcaac ccgagtgtct cttcggaaaa cccgccagcc
     5761 tccagagcgg atcgccagcg gcgccatcac caagggcgtg gtcctggaca gcaccgaggc
     5821 gctgtgcctc gccatctcta ggaactccga gcagatggcc agccacagcg cagtgctgga
     5881 ggccggcaaa aacctctact cgttctgcgt gagctatgtg gattccatcc agcaaatgag
     5941 gaacaagttt gccttccgag aggccatcaa caaactggag aataatctcc gggagcttca
     6001 gatctgcccg gcgacagcag gcagtggtcc agcggccact caggacttca gcaagctcct
     6061 cagttcggtg aaggaaatca gtgacatagt gcagaggtag cagcagtcag gggtcaggtg
     6121 tcaggcccgt cggagctgcc tgcagcacat gcgggctcgc ccatacccgt gacagtggct
     6181 gacaagggac tagtgagtca gcaccttggc ccaggagctc tgcgccaggc agagctgagg
     6241 gccctgtgga gtccagctct actacctacg tttgcaccgc ctgccctccc gcaccttcct
     6301 cctccccgct ccgtctctgt cctcgaattt tatctgtgga gttcctgctc cgtggactgc
     6361 agtcggcatg ccaggacccg ccagccccgc tcccacctag tgccccagac tgagctctcc
     6421 aggccaggtg ggaacggctg atgtggactg tctttttcat ttttttctct ctggagcccc
     6481 tcctcccccg gctgggcctc cttcttccac ttctccaaga atggaagcct gaactgaggc
     6541 cttgtgtgtc aggccctctg cctgcactcc ctggccttgc ccgtcgtgtg ctgaagacat
     6601 gtttcaagaa ccgcatttcg ggaagggcat gcacgggcat gcacacggct ggtcactctg
     6661 ccctctgctg ctgcccgggg tggggtgcac tcgccatttc ctcacgtgca ggacagctct
     6721 tgatttgggt ggaaaacagg gtgctaaagc caaccagcct ttgggtcctg ggcaggtggg
     6781 agctgaaaag gatcgaggca tggggcatgt cctttccatc tgtccacatc cccagagccc
     6841 agctcttgct ctcttgtgac gtgcactgtg aatcctggca agaaagctgg aattcgttaa
     6901 cctcgagaga tccgcggccg cccctctccc tccccccccc ctaacgttac tggccgaagc
     6961 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     7021 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     7081 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     7141 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     7201 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     7261 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     7321 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     7381 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     7441 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc
     7501 cacaaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     7561 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     7621 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     7681 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     7741 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     7801 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     7861 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     7921 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     7981 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     8041 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     8101 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     8161 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     8221 caagtaactc gagggatccg tcgacctgca gccaagctta tcgataaaat aaaagatttt
     8281 atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc
     8341 ttaagtaacg ccattttgca aggcatggaa aatacataac tgagaataga gaagttcaga
     8401 tcaaggttag gaacagagag acagcagaat atgggccaaa caggatatct gtggtaagca
     8461 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc cgccctcagc
     8521 agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg
     8581 ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc
     8641 gagctcaata aaagagccca caacccctca ctcggcgcgc cagtcctccg atagactgcg
     8701 tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc
     8761 gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat
     8821 gggtaacagt ttcttgaagt tggagaacaa cattctgagg gtaggagtcg aatattaagt
     8881 aatcctgact caattagcca ctgttttgaa tccacatact ccaatactcc tgaaatagtt
     8941 cattatggac agcgcagaaa gagctgggga gaattgtgaa attgttatcc gctcacaatt
     9001 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     9061 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     9121 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     9181 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     9241 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     9301 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     9361 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     9421 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     9481 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     9541 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     9601 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     9661 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     9721 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     9781 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     9841 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     9901 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     9961 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    10021 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    10081 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    10141 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    10201 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    10261 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    10321 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    10381 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    10441 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    10501 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    10561 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    10621 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    10681 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    10741 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    10801 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    10861 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    10921 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    10981 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    11041 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    11101 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
    11161 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
    11221 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
    11281 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
    11341 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
    11401 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
    11461 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
    11521 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc gcaaggaatg
    11581 gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca
    11641 cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt
    11701 cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc
    11761 cggcgtagag gcgattagtc caatttgtta aagacaggat atcagtggtc caggctctag
    11821 ttttgactca acaatatcac cagctgaagc ctatagagta cgagccatag ataaaataaa
    11881 agattttatt tagtctccag aaaaaggggg g
//
