LOCUS       sequence-442487-       10748 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  includes 2 UAS cassettes and 1 heat-shock inducible hsp70 casette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10748)
  TITLE     Multigenic Drosophila vectors and transgenic lines for 
            recombinase-directed fluorescent labeling and lineage tracing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 2xUAS-1xhsp70-attB
FEATURES             Location/Qualifiers
     source          1..10748
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..432
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(675..693)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3201..3332,
                     3403..4017)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4800..4894
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        4913..5151
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     intron          5295..5360
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5490..5510
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5782..5916
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     intron          6497..6562
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6692..6712
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6984..7118
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    7134..7228
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        7247..7485
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     intron          7622..7687
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7817..7837
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8109..8243
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    8272..8341
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(8560..8578)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8599..8615)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8599..8615)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8612..8634)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8623..8639
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8647..8653,8654..8671,8672..8677))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8692..8713
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8830..8847)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9001..9589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9081..9100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9760..10551,10552..10620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10383..10402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10621..10725)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacamktyc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccaa gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggctctaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcctt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agtattacat aacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgccattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatctggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagata gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaagat aaataaattt catttaactc gcgaatatta atgagatgcg
     3181 agtaacattt taatttgcag atggttgcca tcttgattgg cctcatcttt ttgggccaac
     3241 aactcacgca agtgggtgtg atgaatatca acggagccat cttcctcttc ctgaccaaca
     3301 tgacctttca aaacgtcttt gccacgataa atgtaagtca tgtttagaat acatttgcat
     3361 ttcaataatt tactaacttt ctaatgaatc gattcgattt aggtgttcac ctcagagctg
     3421 ccagttttta tgagggaggc ccgaagtcga ctttatcgct gtgacacata ctttctgggc
     3481 aaaacgattg ccgaattgcc gctttttctc acagtgccac tggtcttcac ggcgattgcc
     3541 tatccgatga tcggactgcg ggccggagtg ctgcacttct tcaactgcct ggcgctggtc
     3601 actctggtgg ccaatgtgtc aacgtccttc ggatatctaa tatcctgcgc cagctcctcg
     3661 acctcgatgg cgctgtctgt gggtccgccg gttatcatac cattcctgct ctttggcggc
     3721 ttcttcttga actcgggctc ggtgccagta tacctcaaat ggttgtcgta cctctcatgg
     3781 ttccgttacg ccaacgaggg tctgctgatt aaccaatggg cggacgtgga gccgggcgaa
     3841 attagctgca catcgtcgaa caccacgtgc cccagttcgg gcaaggtcat cctggagacg
     3901 cttaacttct ccgccgccga tctgccgctg gactacgtgg gtctggccat tctcatcgtg
     3961 agcttccggg tgctcgcata tctggctcta agacttcggg cccgacgcaa ggagtagccg
     4021 acatatatcc gaaataactg cttgtttttt ttttttacca ttattaccat cgtgtttact
     4081 gtttattgcc ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa acaagatatg
     4141 acctatagaa tacaagtatt tccccttcga acatccccac aagtagactt tggatttgtc
     4201 ttctaaccaa aagacttaca cacctgcata ccttacatca aaaactcgtt tatcgctaca
     4261 taaaacaccg ggatatattt tttatataca tacttttcaa atcgcgcgcc ctcttcataa
     4321 ttcacctcca ccacaccacg tttcgtagtt gctctttcgc tgtctcccac ccgctctccg
     4381 caacacattc accttttgtt cgacgacctt ggagcgactg tcgttagttc cgcgcgattc
     4441 ggttcgctca aatggttccg agtggttcat ttcgtctcaa tagaaattag taataaatat
     4501 ttgtatgtac aatttatttg ctccaatata tttgtatata tttccctcac agctatattt
     4561 attctaattt aatattatga ctttttaagg taattttttg tgacctgttc ggagtgatta
     4621 gcgttacaat ttgaactgaa agtgacatcc agtgtttgtt ccttgtgtag atgcatctca
     4681 aaaaaatggt gggcataata gtgttgttta tatatatcaa aaataacaac tataataata
     4741 agaatacatt taatttagaa aatgcttgga tttcactgga actaggctag cctgcaggtc
     4801 ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga
     4861 gtactgtcct ccgagcggag tactgtcctc cgagcggaga ctctagcgag cgccggagta
     4921 taaatagagg cgcttcgtct acggagcgac aattcaattc aaacaagcaa agtgaacacg
     4981 tcgctaagcg aaagctaagc aaataaacaa gcgcagctga acaagctaaa caatctgcag
     5041 taaagtgcaa gttaaagtga atcaattaaa agtaaccagc aaccaagtaa atcaactgca
     5101 actactgaaa tctgccaaga agtaattatt gaatacaaga agagaactct gaatagggaa
     5161 ttgggaattg gccggccata tgaccggtga tacttaatta actgaagacg tcctagagga
     5221 tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
     5281 tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc
     5341 taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga
     5401 atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg
     5461 ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca
     5521 aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc
     5581 ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta
     5641 tggaaaaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
     5701 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     5761 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     5821 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     5881 tccaaactca tcaatgtatc ttatcatgtc tggatccaag cttctagaat cccaaaacaa
     5941 actagttgtt gtggtaggtc atttgtttgg cagaaagaaa actcgagaaa tttctctggc
     6001 cgttattcgt tattctctct tttctttttg ggtctctccc tctctgcact aatgctctct
     6061 cactctgtca cacagtaaac ggcatactgc tctcgttggt tcgagagagc gcgcctcgaa
     6121 tgttcgcgaa aagagcgccg gagtataaat agaggcgctt cgtctacgga gcgacaattc
     6181 aattcaaaca agcaaagtga acacgtcgct aagcgaaagc taagcaaata aacaagcgca
     6241 gctgaacaag ctaaacaatc tgcagtaaag tgcaagttaa agtgaatcaa ttaaaagtaa
     6301 ccagcaacca agtaaatcaa ctgcaactac tgaaatctgc caagaagtaa ttattgaata
     6361 caagaagagg gcgcgccata tgcgtacgga tacgcgatcg cctgaagttt aaacctagag
     6421 gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga
     6481 gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat
     6541 tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg
     6601 gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga
     6661 ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc
     6721 caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac
     6781 tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat
     6841 tatggaaaaa tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat
     6901 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     6961 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     7021 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     7081 tgtccaaact catcaatgta tcttatcatg tctggatccg catgcctgca ggtcggagta
     7141 ctgtcctccg agcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg
     7201 tcctccgagc ggagtactgt cctccgagcg gagactctag cgagcgccgg agtataaata
     7261 gaggcgcttc gtctacggag cgacaattca attcaaacaa gcaaagtgaa cacgtcgcta
     7321 agcgaaagct aagcaaataa acaagcgcag ctgaacaagc taaacaatct gcagtaaagt
     7381 gcaagttaaa gtgaatcaat taaaagtaac cagcaaccaa gtaaatcaac tgcaactact
     7441 gaaatctgcc aagaagtaat tattgaatac aagaagagaa ctctgaatag ggaattggga
     7501 attcgttaac agatctgcgg ccgcggctcg agggtacctc tagaggatct ttgtgaagga
     7561 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa
     7621 ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt
     7681 attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga
     7741 ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc
     7801 tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc
     7861 agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc
     7921 tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattt
     7981 gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac
     8041 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     8101 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     8161 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     8221 atgtatctta tcatgtctgg atccactagt gtcgacgatg taggtcacgg tctcgaagcc
     8281 gcggtgcggg tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat
     8341 ctggtccatc atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg
     8401 caccgggaag ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg
     8461 tgcgacggcg tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac
     8521 ggcgggcatg tcgacactag ttctagccag cttttgttcc ctttagtgag ggttaatttc
     8581 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     8641 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     8701 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     8761 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     8821 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     8881 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     8941 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9001 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9061 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     9241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     9301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     9361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     9421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     9481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     9541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     9601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     9661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     9721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     9781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     9841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     9901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     9961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    10021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    10081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    10141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    10201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    10261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    10321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    10381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    10441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    10501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    10561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    10621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    10681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    10741 agtgccac
//
