LOCUS       sequence-442496-        9398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV-BI30 Rep-Cap plasmid for production of AAV-BI30, a capsid with 
            tropism for CNS endothelial cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9398)
  AUTHORS   Krolak T, Chan KY, Kaplan L, Huang Q, Wu J, Zheng Q, Kozareva V, 
            Beddow T, Tobey IG, Pacouret S, Chen AT, Chan YA, Ryvkin D, Gu C, 
            Deverman BE
  TITLE     A High-Efficiency AAV for Endothelial Cell Transduction Throughout 
            the Central Nervous System.
  JOURNAL   Nat Cardiovasc Res. 2022 Apr;1(4):389-400. doi: 
            10.1038/s44161-022-00046-4. Epub 2022 Apr 13.
  PUBMED    35571675
REFERENCE   2  (bases 1 to 9398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44161-022-00046-4"; journalName: "Nat Cardiovasc Res"; 
            date: "2022-04"; volume: "1"; issue: "4"; pages: "389-400"
COMMENT     Sequence Label: kiCAP-AAV-BI30
FEATURES             Location/Qualifiers
     source          1..9398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2759..2869
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     promoter        complement(2908..2926)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2909..2926)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2946..2963)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2952..3176
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    3238..3256
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3275..3293
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3312..3330
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3349..3367
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3386..3404
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3423..3441
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3460..3478
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3497..3515
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             6232..6243
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(6584..6604)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6584..6602)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6623..6639)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6623..6639)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6636..6658)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6647..6663
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6671..6677,6678..6695,6696..6701))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6716..6737
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6854..6871)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7025..7613)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7105..7124)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7784..8593)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8507..8526
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(8594..8698)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8724..9179)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8861..8882)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9073..9092
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9306..9328
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9320..9337
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9321..9337
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9347..9366
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9347..9365
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtcgacacgc gtggaggggt ggagtcgtga cgtgaattac gtcatagggt tagggaggtc
       61 ctgtattaga ggtcacgtga gtgttttgcg acattttgcg acaccatgtg gtcacgctgg
      121 gtatttaagc ccgagtgagc acgcagggtc tccattttga agcgggaggt ttgaacgcgc
      181 agccgccatg ccggggtttt acgagattgt gattaaggtc cccagcgacc ttgacgagca
      241 tctgcccggc atttctgaca gctttgtgaa ctgggtggcc gagaaggaat gggagttgcc
      301 gccagattct gacatggatc tgaatctgat tgagcaggca cccctgaccg tggccgagaa
      361 gctgcagcgc gactttctga cggaatggcg ccgtgtgagt aaggccccgg aggctctttt
      421 ctttgtgcaa tttgagaagg gagagagcta cttccacatg cacgtgctcg tggaaaccac
      481 cggggtgaaa tccatggttt tgggacgttt cctgagtcag attcgcgaaa aactgattca
      541 gagaatttac cgcgggatcg agccgacttt gccaaactgg ttcgcggtca caaagaccag
      601 aaatggcgcc ggaggcggga acaaggtggt ggatgagtgc tacatcccca attacttgct
      661 ccccaaaacc cagcctgagc tccagtgggc gtggactaat atggaacagt atttaagcgc
      721 ctgtttgaat ctcacggagc gtaaacggtt ggtggcgcag catctgacgc acgtgtcgca
      781 gacgcaggag cagaacaaag agaatcagaa tcccaattct gatgcgccgg tgatcagatc
      841 aaaaacttca gccaggtaca tggagctggt cgggtggctc gtggacaagg ggattacctc
      901 ggagaagcag tggatccagg aggaccaggc ctcatacatc tccttcaatg cggcctccaa
      961 ctcgcggtcc caaatcaagg ctgccttgga caatgcggga aagattatga gcctgactaa
     1021 aaccgccccc gactacctgg tgggccagca gcccgtggag gacatttcca gcaatcggat
     1081 ttataaaatt ttggaactaa acgggtacga tccccaatat gcggcttccg tctttctggg
     1141 atgggccacg aaaaagttcg gcaagaggaa caccatctgg ctgtttgggc ctgcaactac
     1201 cgggaagacc aacatcgcgg aggccatagc ccacactgtg cccttctacg ggtgcgtaaa
     1261 ctggaccaat gagaactttc ccttcaacga ctgtgtcgac aagatggtga tctggtggga
     1321 ggaggggaag atgaccgcca aggtcgtgga gtcggccaaa gccattctcg gaggaagcaa
     1381 ggtgcgcgtg gaccagaaat gcaagtcctc ggcccagata gacccgactc ccgtgatcgt
     1441 cacctccaac accaacatgt gcgccgtgat tgacgggaac tcaacgacct tcgaacacca
     1501 gcagccgttg caagaccgga tgttcaaatt tgaactcacc cgccgtctgg atcatgactt
     1561 tgggaaggtc accaagcagg aagtcaaaga ctttttccgg tgggcaaagg atcacgtggt
     1621 tgaggtggag catgaattct acgtcaaaaa gggtggagcc aagaaaagac ccgcccccag
     1681 tgacgcagat ataagtgagc ccaaacgggt gcgcgagtca gttgcgcagc catcgacgtc
     1741 agacgcggaa gcttcgatca actacgcgga caggtaccaa aacaaatgtt ctcgtcacgt
     1801 gggcatgaat ctgatgctgt ttccctgcag acaatgcgag agactgaatc agaattcaaa
     1861 tatctgcttc actcacggtg tcaaagactg tttagagtgc tttcccgtgt cagaatctca
     1921 acccgtttct gtcgtcaaaa aggcgtatca gaaactgtgc tacattcatc acatcatggg
     1981 aaaggtgcca gacgcttgca ctgcttgcga cctggtcaat gtggacttgg atgactgtgt
     2041 ttctgaacaa taaatgactt aaaccaggta ttgctgccga tggttatctt ccagattggc
     2101 tcgaggacaa ccttagtgaa ctagtgccac catgagtcgg ctggataaat ctaaagtcat
     2161 aaacggcgct ctggaattac tcaatgaagt cggtatcgaa ggcctgacga caaggaaact
     2221 cgctcaaaag ctgggagttg agcagcctac cctgtactgg cacgtgaaga acaagcgggc
     2281 cctgctcgat gccctggcca tcgagatgct ggacaggcat catacccact tctgccccct
     2341 ggaaggcgag tcatggcaag actttctgcg gaacaacgcc aagtcattcc gctgtgctct
     2401 cctctcacat cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag agaaacagta
     2461 cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc
     2521 actgtacgct ctgtccgccg tgggccactt tacactgggc tgcgtattgg aggaacagga
     2581 gcatcaagta gcaaaagagg aaagagagac acctaccacc gattctatgc ccccacttct
     2641 gagacaagca attgagctgt tcgaccggca gggagccgaa cctgccttcc ttttcggcct
     2701 ggaactaatc atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg ggccggccga
     2761 cgcccttgac gattttgact tagacatgct cccagccgat gcccttgacg actttgacct
     2821 tgatatgctg cctgctgacg ctcttgacga ttttgacctt gacatgctcc ccgggtaaat
     2881 gcatgaattc gatctagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg
     2941 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     3001 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     3061 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     3121 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     3181 tgaggcggaa agaaccagct ggggctcgaa tcaagctatc aagtgccacc tgacgtctcc
     3241 ctatcagtga tagagaagtc gacacgtctc gagctcccta tcagtgatag agaaggtacg
     3301 tctagaacgt ctccctatca gtgatagaga agtcgacacg tctcgagctc cctatcagtg
     3361 atagagaagg tacgtctaga acgtctccct atcagtgata gagaagtcga cacgtctcga
     3421 gctccctatc agtgatagag aaggtacgtc tagaacgtct ccctatcagt gatagagaag
     3481 tcgacacgtc tcgagctccc tatcagtgat agagaaggta ccccctatat aagcagagag
     3541 atctgttcaa atttgaactg actaagcggc tcccgccaga ttttggcaag attactaagc
     3601 aggaagtcaa ggactttttt gcttgggcaa aggtcaatca ggtgccggtg actcacgagt
     3661 ttaaagttcc cagggaattg gcgggaacta aaggggcgga gaaatctcta aaacgcccac
     3721 tgggtgacgt caccaatact agctataaaa gtctggagaa gcgggccagg ctctcatttg
     3781 ttcccgagac gcctcgcagt tcagacgtga ctgttgatcc cgctcctctg cgaccgctag
     3841 cttcgatcaa ctacgcggac aggtaccaaa acaaatgttc tcgtcacgtg ggcatgaatc
     3901 tgatgctgtt tccctgcaga caatgcgaga gactgaatca gaattcaaat atctgcttca
     3961 ctcacggtgt caaagactgt ttagagtgct ttcccgtgtc agaatctcaa cccgtttctg
     4021 tcgtcaaaaa ggcgtatcag aaactgtgct acattcatca catcatggga aaggtgccag
     4081 acgcttgcac tgcttgcgac ctggtcaatg tggacttgga tgactgtgtt tctgaacaat
     4141 aaatgactta aaccaggtat ggctgccgat ggttatcttc cagattggct cgaggacaac
     4201 cttagtgaag gaattcgcga gtggtgggct ttgaaacctg gagcccctca acccaaggca
     4261 aatcaacaac atcaagacaa cgctcgaggt cttgtgcttc cgggttacaa ataccttgga
     4321 cccggcaacg gactcgacaa gggggagccg gtcaacgcag cagacgcggc ggccctcgag
     4381 cacgacaagg cctacgacca gcagctcaag gccggagaca acccgtacct caagtacaac
     4441 cacgccgacg ccgagttcca ggagcggctc aaagaagata cgtcttttgg gggcaacctc
     4501 gggcgagcag tcttccaggc caaaaagagg cttcttgaac ctcttggtct ggttgaggaa
     4561 gcggctaaga cggctcctgg aaagaagagg cctgtagagc agtctcctca ggaaccggac
     4621 tcctccgcgg gtattggcaa atcgggtgca cagcccgcta aaaagagact caatttcggt
     4681 cagactggcg acacagagtc agtcccagac cctcaaccaa tcggagaacc tcccgcagcc
     4741 ccctcaggtg tgggatctct tacaatggct tcaggtggtg gcgcaccagt ggcagacaat
     4801 aacgaaggtg ccgatggagt gggtagttcc tcgggaaatt ggcattgcga ttcccaatgg
     4861 ctgggggaca gagtcatcac caccagcacc cgaacctggg ccctgcccac ctacaacaat
     4921 cacctctaca agcaaatctc caacagcaca tctggaggat cttcaaatga caacgcctac
     4981 ttcggctaca gcaccccctg ggggtatttt gacttcaaca gattccactg ccacttctca
     5041 ccacgtgact ggcagcgact catcaacaac aactggggat tccggcctaa gcgactcaac
     5101 ttcaagctct tcaacattca ggtcaaagag gttacggaca acaatggagt caagaccatc
     5161 gccaataacc ttaccagcac ggtccaggtc ttcacggact cagactatca gctcccgtac
     5221 gtgctcgggt cggctcacga gggctgcctc ccgccgttcc cagcggacgt tttcatgatt
     5281 cctcagtacg ggtatctgac gcttaatgat ggaagccagg ccgtgggtcg ttcgtccttt
     5341 tactgcctgg aatatttccc gtcgcaaatg ctaagaacgg gtaacaactt ccagttcagc
     5401 tacgagtttg agaacgtacc tttccatagc agctacgctc acagccaaag cctggaccga
     5461 ctaatgaatc cactcatcga ccaatacttg tactatctct ctagaactat taacggttct
     5521 ggacagaatc aacaaacgct aaaattcagt gtggccggac ccagcaacat ggctgtccag
     5581 ggaagaaact acatacctgg acccagctac cgacaacaac gtgtctcaac cactgtgact
     5641 caaaacaaca acagcgaatt tgcttggcct ggagcttctt cttgggctct caatggacgt
     5701 aatagcttga tgaatcctgg acctgctatg gccagccaca aagaaggaga ggaccgtttc
     5761 tttcctttgt ctggatcttt aatttttggc aaacaaggaa ctggaagaga caacgtggat
     5821 gcggacaaag tcatgataac caacgaagaa gaaattaaaa ctactaaccc ggtagcaacg
     5881 gagtcctatg gacaagtggc cacaaaccac cagagtgccc aaaacaactc aacccgcggc
     5941 ggcgcacagg cgcagaccgg ttgggttcaa aaccaaggaa tacttccggg tatggtttgg
     6001 caggacagag atgtgtacct gcaaggaccc atttgggcca aaattcctca cacggacggc
     6061 aactttcacc cttctccgct gatgggaggg tttggaatga agcacccgcc tcctcagatc
     6121 ctcatcaaaa acacacctgt acctgcggat cctccaacgg ccttcaacaa ggacaagctg
     6181 aactctttca tcacccagta ttctactggc caagtcagcg tggagatcga gtgggagctg
     6241 cagaaggaaa acagcaagcg ctggaacccg gagatccagt acacttccaa ctattacaag
     6301 tctaataatg ttgaatttgc tgttaatact gaaggtgtat atagtgaacc ccgccccatt
     6361 ggcaccagat acctgactcg taatctgtaa ttgcttgtta atcaataaac cgtttaattc
     6421 gtttcagttg aactttggtc tctgcgtatt tctttcttat ctagtttcca tggctacgta
     6481 gataagtagc atggcgggtt aatcattaac tacagatatc catcacactg gcggccgctc
     6541 gactagagcg gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa
     6601 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     6661 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     6721 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     6781 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6841 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6901 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6961 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     7021 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7081 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7141 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7201 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7261 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7321 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7381 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7441 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     7501 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7561 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7621 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7681 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7741 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttagaaa aactcatcga
     7801 gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa
     7861 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct
     7921 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt
     7981 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg
     8041 gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat
     8101 caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa
     8161 atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga
     8221 acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga
     8281 atgctgtttt tccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa
     8341 aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat
     8401 ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg
     8461 gcttcccata caagcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt
     8521 tatacccata taaatcagca tccatgttgg aatttaatcg cggcctcgac gtttcccgtt
     8581 gaatatggct catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     8641 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     8701 catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt
     8761 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     8821 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     8881 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     8941 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     9001 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     9061 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     9121 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     9181 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     9241 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     9301 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact
     9361 atagggcgaa ttgggtaccg ggccccccct cgatcgag
//
