LOCUS       sequence-442522-        9259 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposon plasmid for doxycycline inducible expression of 
            KCTD19.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9259)
  AUTHORS   Pierson Smela M, Adams J, Ma C, Breimann L, Widocki U, Shioda T, 
            Church GM
  TITLE     Induction of Meiosis from Human Pluripotent Stem Cells
  JOURNAL   bioRxiv 2024.05.31.596483
REFERENCE   2  (bases 1 to 9259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.31.596483"
COMMENT     Sequence Label: pPB-cT3G-cERP2-KCTD19
FEATURES             Location/Qualifiers
     source          1..9259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    16..34
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    52..70
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    88..106
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    124..142
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    160..178
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    196..214
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    232..250
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     277..301
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    367..391
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3173..3197)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3199..3225
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    3392..3526
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   3798..3832
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(3843..3860)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3843..3859)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3852..3874)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4068..4087)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4189..4211
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4250..4268)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4336..4440
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4441..4509,4510..5301)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4659..4678)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5472..6060
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5961..5980
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6214..6231
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6437..6458
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6473..6478,6479..6496,6497..6503)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6511..6527
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6516..6538
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6535..6551
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6535..6551
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   6577..6639
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    6815..6896
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(7613..7669)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(7679..8422)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(8423..8446)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      8446..8455
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(8475..8686)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        8687..9066
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tcacctcgag tttactccct atcagtgata gagaacgtat gaagagttta ctccctatca
       61 gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt ataaggagtt
      121 tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt gatagagaac
      181 gtatctacag tttactccct atcagtgata gagaacgtat atccagttta ctccctatca
      241 gtgatagaga acgtataagc ttgcctatat aagcagagct cgtttagtga accgtcagat
      301 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
      361 cctaccacaa gtttgtacaa aaaagttggc accatggagg agtctggcat ggctcatgaa
      421 tcagcagagg acttgtttca tttcaacgta gggggctggc atttctcagt tcccagaagc
      481 aaactctctc agtttccaga ctccctgctg tggaaagagg cttcagcctt gacctcttca
      541 gaaagccaga ggctatttat cgacagagat ggttccacat ttaggcacgt gcactattac
      601 ctctacacct ccaaactctc cttctccagt tgtgcagaac tgaacttgct gtatgagcaa
      661 gcattgggtt tgcagctgat gcctttgctg cagactctag ataacctgaa ggaagggaaa
      721 caccatctac gcgtacggcc tgcagaccta cctgttgctg agagagcatc tctgaactac
      781 tggcgtacat ggaagtgtat tagcaaaccc tcagaatttc caattaaaag cccagccttt
      841 acaggcctac atgataaggc acctctgggg ctcatggaca cacccctgtt agacacagaa
      901 gaggaggtgc actactgctt cctgccccta gacctggtgg ccaaatatcc cagcctagtg
      961 actgaagaca acctgctgtg gctggctgag acggtggccc tcatcgagtg cgagtgcagc
     1021 gagttccgct tcattgtgaa ttttcttcgc tcacagaaga ttttactacc ggataatttc
     1081 tccaacattg atgtattaga agcagaagtg gaaattctgg aaatccctgc actcactgaa
     1141 gccgtaaggt ggtaccggat gaacatgggt ggctgttccc cgaccacctg ttctcccctg
     1201 agccccggga agggggcccg cacagccagc ctggagtccg tgaaaccgct ctacacaatg
     1261 gccctgggtc tgctggtcaa gtacccggac tctgcgctgg gccagcttcg catcgagagc
     1321 acgctagacg gaagccgact gtacatcaca gggaatggcg tcctctttca gcacgtcaag
     1381 aactggctgg ggacttgccg gctgcccctg acagagacca tttccgaggt atatgagctc
     1441 tgtgccttcc tagacaaaag ggacatcacc tacgagccaa tcaaagttgc tttgaagact
     1501 catctggagc caaggacttt ggcacccatg gatgtgctca atgagtggac ggcagagatc
     1561 actgtgtatt ccccacaaca gatcatcaaa gtgtatgttg gaagccactg gtacgcaacc
     1621 accctgcaga cactgctgaa gtatccagaa ctgctgtcca accctcagag agtgtactgg
     1681 atcacatatg gacaaaccct gctcatccac ggggatggcc agatgttccg acacattctc
     1741 aacttcctga gacttggcaa actgttttta ccatctgaat ttaaggaatg gcccctcttc
     1801 tgccaggagg tggaggaata ccacattcca tccctctcag aagcccttgc acaatgtgaa
     1861 gcatacaagt catggactca ggagaaagaa tctgaaaatg aagaagcttt ttccatcagg
     1921 aggctgcatg tggtgacaga agggccaggg tcactggtgg agttcagtag agacactaaa
     1981 gaaaccacag cctacatgcc tgtggacttc gaagactgca gtgacaggac tccatggaac
     2041 aaggctaagg gaaacctggt caggtccaac cagatggatg aggctgagca gtacactcgg
     2101 cccatccagg tgtccctatg ccgaaatgcc aagagggctg gcaaccctag cacatactca
     2161 cactgccgtg gcttgtgtac caatcctgga cactggggga gccaccctga gagcccccca
     2221 aagaagaaat gcaccacaat caacctcaca cagaaatctg aaaccaaaga ccctcccgcc
     2281 actcccatgc aaaaactcat ctccctggtg agagaatggg acatggtcaa ttgcaaacag
     2341 tgggaattcc agccactgac agccacacgg agcagcccct tggaggaggc caccctgcag
     2401 ctccccttgg gaagcgaggc tgcttcccag cccagcacct cagctgcctg gaaagcccat
     2461 tccacagcct cagagaagga tccaggacca caggcagggg ctggagctgg agcgaaagac
     2521 aaggggccag agccaacctt caagccatac ttacccccaa aaagagctgg caccctgaag
     2581 gactggagca agcagaggac caaggagaga gaaagccctg cccctgagca gcctctgccc
     2641 gaggccagtg aggtggacag cctaggggtt atcctcaaag tgactcaccc ccccgtggtg
     2701 ggcagcgatg gcttctgcat gttctttgag gacagcatca tctataccac ggagatggac
     2761 aacctcaggc acacaacacc cacagccagt ccccagcccc aagaagtgac tttcctgagt
     2821 ttctctctgt cctgggaaga gatgttttat gcacagaaat gtcactgctt cctggctgac
     2881 atcatcatgg attccatcag gcaaaaggac cccaaagcca tcacagccaa ggtggtctcc
     2941 ctggccaatc ggctgtggac cctgcacatc agccccaagc agtttgtggt agatttgctg
     3001 gccatcaccg gcttcaagga tgaccggcac acccaggagc gcctgtacag ctgggtggag
     3061 cttacactgc ccttcgccag gaaatatggc cgatgcatgg acctgctcat ccagaggggc
     3121 ctgtctaggt ctgtctctta ctccatcctg ggaaagtacc tacaagagga cttgccaact
     3181 ttcttgtaca aagtggtata cccatacgat gttccagatt acgcttagta atgagaaata
     3241 ctcagaagat gtcactcacc atagtccgca gtgaaagata aatgatccca tgtgacaaat
     3301 tcccatcgtt ttagagctag aaatagcaag taggccactg agcaagaact atgaatgaga
     3361 tctttagaca tattgcggcc gctttctccg cgatccagac atgataagat acattgatga
     3421 gtttggacaa accaaaacta gaatgcagtg aaaaaaatgc cttatttgtg aaatttgtga
     3481 tgctattgcc ttatttgtaa ccattataag ctgcaataaa caagttatct ataacaagaa
     3541 aatatatata taataagtta tcacgtaagt agaacatgaa ataacaatat aattatcgta
     3601 tgagttaaat cttaaaagtc acgtaaaaga taatcatgcg tcattttgac tcacgcggtc
     3661 gttatagttc aaaatcagtg acacttaccg cattgacaag cacgcctcac gggagctcca
     3721 agcggcgact gagatgtcct aaatgcacag cgacggattc gcgctattta gaaagagaga
     3781 gcaatatttc aagaatgcat gcgtcaattt tacgcagact atctttctag ggttaagaat
     3841 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     3901 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     3961 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
     4021 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
     4081 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     4141 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccatgtctcc gggagctgca
     4201 tgtgtcagag gttttcaccg tcatcaccga aacacgcgag ttcgaaaggg cctcgtgata
     4261 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     4321 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4381 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4441 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4501 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4561 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4621 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4681 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4741 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     4801 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     4861 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     4921 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     4981 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5041 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5101 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtggttca
     5161 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5221 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5281 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5341 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5401 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5461 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5521 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5581 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     5641 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5701 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5761 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5821 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5881 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5941 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6001 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6061 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6121 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     6181 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggat
     6241 gagcgcccaa tacgcaaacc gcctctcacc gcgcgttggc cgattcatta atgcagctgg
     6301 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaaaccg cctctccccg
     6361 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     6421 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     6481 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     6541 acagctatga ccatgattac gccaaggtcg acttaaccct agaaagataa tcatattgtg
     6601 acgtacgtta aagataatca tgcgtaaaat tgacgcatgt gttttatcgg tctgtatatc
     6661 gaggtttatt tattaatttg aatagatatt aagttttatt atatttacac ttacatacta
     6721 ataataaatt caacaaacaa tttatttatg tttatttatt tattaaaaaa aaacaaaaac
     6781 tcaaaatttc ttctataaag taacaaaact tttagcagtg aaaaaaatgc tttatttgtg
     6841 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     6901 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     6961 gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcctctggag attcatgcgc
     7021 caggttttct ggtcatgcac cacgttcgtg ggccctcagg aacctccacg tcagcggtaa
     7081 ctgtgaagcc cagtcgctcg taaaaaggaa gattccgggg agcactggtt tcgaggaacg
     7141 ccggcacgcc agcccgttcc gcggcctcaa caccaggaag aacgacagcg cttcccaaac
     7201 ctttaccttg gtgatctggg ctgacaccga cagtggcaag aaaccacgca ggctcctttg
     7261 gtctatgagg agccagcaag ccctccattt gctgttgtgc cgccaaccta ctccctgaca
     7321 gctcggccat ccgcggacct atttccgcga acactgcgcc ggcttcgacg ctttccggag
     7381 tagtccagac cgcaactgcg gcaccatcgt ccgcaaccca aactttacca atgtccagac
     7441 ccacgcgcgt aagaaacagc tcttgcaact cagttaccct ctctatgtgt ctgtccggat
     7501 ctacggtatg gcgggtcgct ggataatctg caaatgctgc ggcaagtgtt cggacagccc
     7561 gtggtacatc gtcacgggta gcgagcctaa cagtgggctt atattccgtc atggggccgg
     7621 ggttctcctc cacgtcgccg gcctgcttca gcaggctgaa gttggtggcg ccgctgcccc
     7681 cggggagcat gtcaaggtca aaatcgtcaa gagcgtcagc aggcagcata tcaaggtcaa
     7741 agtcgtcaag ggcatcggct gggagcatgt ctaagtcaaa atcgtcaagg gcgtcggtcg
     7801 gcccgccgct ttcgcacttt agctgtttct ccaggccaca tatgattagt tccaggccga
     7861 aaaggaaggc aggttcggct ccctgccggt cgaacagctc aattgcttgt ttcagaagtg
     7921 ggggcataga atcggtggta ggtgtctctc tttcctcttt tgctacttga tgctcctgtt
     7981 cctccaatac gcagcccagt gtaaagtggc ccacggcgga cagagcgtac agtgcgttct
     8041 ccagggagaa gccttgctga cacaggaacg cgagctgatt ttccagggtt tcgtactgtt
     8101 tctctgttgg gcgggtgccg agatgcactt tagccccgtc acgatgtgag aggagagcac
     8161 agcggtatga cttggcgttg ttccgcagaa agtcttgcca tgactcgcct tccagggggc
     8221 aggagtgggt atgatgcctg tccagcatct cgattggcag ggcatcgagc agggcccgct
     8281 tgttcttcac gtgccagtac agggtaggct gctcaactcc cagcttttga gcgagtttcc
     8341 ttgtcgtcag gccttcgata ccgactccat tgagtaattc cagagcagag tttatgactt
     8401 tgctcttgtc cagtctagac atcttatcgt catcgtcttt gtaatccatg gtggcggatc
     8461 ccgcgtcacg acacctgtgt tctggcggca aacccgttgc gaaaaagaac gttcacggcg
     8521 actactgcac ttatatacgg ttctccccca ccctcgggaa aaaggcggag ccagtacacg
     8581 acatcacttt cccagtttac cccgcgccac cttctctagg caccggttca attgccgacc
     8641 cctcccccca acttctcggg cactgtgggc catgtgcgct ctgccccatg gtaatagcca
     8701 tgactaatac gtagatgtac tgccaagtag gaaagtgcca taaggtcatg tactgggcat
     8761 aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat
     8821 acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat
     8881 ggaaagtgcc tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg
     8941 ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca
     9001 tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta gtcaataatc
     9061 aatgtcataa gctctaatcg cataatcgag ctaagagcga ttaatatgct tagctagctt
     9121 agctaagcta gatttctcta ttctcccaca atatatagca gctattagct tagcagtccg
     9181 aatacgatcg tccacgatcc ataagcgtaa cacatttgag tagcgactga cgggcaccgg
     9241 agccactcga gtggaatta
//
