LOCUS       sequence-442537-        3045 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains Level 0 Part: replication origin (SH71rep) for the 
            construction of Level 1 plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Li X, Schonberg PY, Wucherpfennig T, Hinze C, Sulaj F, Henle T, 
            Mascher T
  TITLE     Development of a Golden Gate Assembly-Based Genetic Toolbox for 
            Lactiplantibacillus plantarum and Its Application for Engineering 
            Monoterpenoid Biosynthesis.
  JOURNAL   ACS Synth Biol. 2024 Sep 20;13(9):2764-2779. doi: 
            10.1021/acssynbio.4c00075. Epub 2024 Sep 10.
  PUBMED    39254046
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00075"; journalName: "ACS Synth Biol"; date: 
            "2024-09-20- 20"; volume: "13"; issue: "9"; pages: "2764-2779"
COMMENT     Sequence Label: pLPO2
FEATURES             Location/Qualifiers
     source          1..3045
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(294..305)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(2009..2103)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2124..2783)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2698..2717
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2945..2963
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2967..3010)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3013..3034
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ctcgagctcg tttgatttcg ctttcgatag aacaatcaaa gcgagaataa ggaagataaa
       61 tcccataagg gcgggagcag aatgtccgag actaattcat ggatcgattt tttattaaaa
      121 cgtcacaaaa tcgtttctgt gacgttttag cgtttatttc gtttagttat cggcataatc
      181 gttaaaacag gcgttatcgt agcgtaaaag cccttgagcg tagcgtggct ttgcagcgaa
      241 gatgttgtct gttagattat gaaagccgat gactgaatga aataataagc gcagcgtcct
      301 tctatttcgg ttggaggagg ctcaagggag tttgagggaa tgaaattccc tcatgggttt
      361 gattttaaaa attgcttgca attttgccga gcggtagcgc tggaaaattt ttgaaaaaaa
      421 tttggaattt ggaaaaaaat ggggggaaag gaagcgaatt ttgcttccgt actacgaccc
      481 cccattaagt gccgagtgcc aatttttgtg ccaaaaacgc tctatcccaa ctggctcaag
      541 ggtttgaggg gtttttcaat cgccaacgaa tcgccaacgt tttcgccaac gttttttata
      601 aatctatatt taagtagctt tattgttgtt tttatgatta caaagtgata cactaatttt
      661 ataaaattat ttgattggag ttttttaaat ggtgatttca gaatcgaaaa aaagagttat
      721 gatttctctg acaaaagagc aagataaaaa attaacagat atggcgaaac aaaaaggttt
      781 ttcaaaatct gcggttgcgg cgttagctat agaagaatat gcaagaaagg aatcagaaca
      841 aaaaaaataa gcgaaagctc gcgtttttag aaggatacga gttttcgcta cttgtttttg
      901 ataaggtaat tatatcatgg ctattaaaaa tactaaagct agaaattttg gatttttatt
      961 atatcctgac tcaattccta atgattggaa agaaaaatta gagagtttgg gcgtatctat
     1021 ggctgtcagt ctttacacga tatggacgaa aaaaaagata aagatacatg gaatagtagt
     1081 gatgttatac gaaatggaaa gcactataaa aaaccacact atcacgttat atatattgca
     1141 cgaaatcctg taacaataga aagcgttagg aacaagatta agcgaaaatt ggggaatagt
     1201 tcagttgctc atgttgagat acttgattat atcaaaggtt catatgaata tttgactcat
     1261 gaatcaaagg acgctattgc taagaataaa catatatacg acaaaaaaga tattttgaac
     1321 attaatgatt ttgatattga ccgctatata acacttgatg aaagccaaaa aagagaattg
     1381 aagaatttac ttttagatat agtggatgac tataatttgg taaatacaaa agatttaatg
     1441 gcttttattc gccttagggg agcggagttt ggaattttaa atacgaatga tgtaaaagat
     1501 attgtttcaa caaactctag cgcctttaga ttatggtttg agggcaatta tcagtgtgga
     1561 tatagagcaa gttatgcaaa ggttcttgat gctgaaacgg gggaaataaa atgacaaaca
     1621 aagaaaaaga gttatttgct gaaaatgagg aattaaaaaa agaaattaag gacttaaaag
     1681 agcgtattga aagatacaga gaaatggaag ttgaattaag tacaacaata gatttattga
     1741 gaggagggat tattgaataa ataaaagccc ccctgacgaa agtcgaaggg ggcttttatt
     1801 ttggtttgat gttgcgatta atagcaatac gattgcaata aacaaaagga tccccttaga
     1861 agcaaactgc ttgagaccca cgttaaggga ttttggtcat gagattatca aaaaggatct
     1921 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     1981 aaacttggtc tgacagctcg aggcttggat tctcaccaat aaaaaacgcc cggcggcaac
     2041 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
     2101 gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcgtcgca gtactgttgt
     2161 aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc
     2221 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
     2281 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
     2341 ttggctgata cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
     2401 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
     2461 tcactccaga gtgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
     2521 acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca
     2581 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
     2641 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
     2701 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
     2761 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca
     2821 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc
     2881 ccgatcaact cgagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     2941 taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa
     3001 ggatgatttc tggaattcgc ggccgcttct agagcgatga gtggt
//
