LOCUS       sequence-442545-       10543 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposon plasmid for doxycycline inducible expression of 
            BRCA1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10543)
  AUTHORS   Pierson Smela M, Adams J, Ma C, Breimann L, Widocki U, Shioda T, 
            Church GM
  TITLE     Induction of Meiosis from Human Pluripotent Stem Cells
  JOURNAL   bioRxiv 2024.05.31.596483
REFERENCE   2  (bases 1 to 10543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.31.596483"
COMMENT     Sequence Label: pPB-cT3G-cERP2-BRCA1
FEATURES             Location/Qualifiers
     source          1..10543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    16..34
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    52..70
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    88..106
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    124..142
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    160..178
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    196..214
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    232..250
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     277..301
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    367..391
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4457..4481)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4483..4509
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    4676..4810
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   5082..5116
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5127..5144)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5127..5143)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5136..5158)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5352..5371)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5473..5495
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5534..5552)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5620..5724
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5725..5793,5794..6585)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5943..5962)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6756..7344
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7245..7264
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7498..7515
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7721..7742
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7757..7762,7763..7780,7781..7787)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7795..7811
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7800..7822
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7819..7835
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7819..7835
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   7861..7923
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    8099..8180
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(8897..8953)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(8963..9706)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(9707..9730)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      9730..9739
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(9759..9970)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        9971..10350
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tcacctcgag tttactccct atcagtgata gagaacgtat gaagagttta ctccctatca
       61 gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt ataaggagtt
      121 tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt gatagagaac
      181 gtatctacag tttactccct atcagtgata gagaacgtat atccagttta ctccctatca
      241 gtgatagaga acgtataagc ttgcctatat aagcagagct cgtttagtga accgtcagat
      301 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
      361 cctaccacaa gtttgtacaa aaaagttggc accatgataa atcagggaac taaccaaacg
      421 gagcagaatg gtcaagtgat gaatattact aatagtggtc atgagaataa aacaaaaggt
      481 gattctattc agaatgagaa aaatcctaac ccaatagaat cactcgaaaa agaatctgct
      541 ttcaaaacga aagctgaacc tataagcagc agtataagca atatggaact cgaattaaat
      601 atccacaatt caaaagcacc taaaaagaat aggctgagga ggaagtcttc taccaggcat
      661 attcatgcgc ttgaactagt agtcagtaga aatctaagcc cacctaattg tactgaattg
      721 caaattgata gttgttctag cagtgaagag ataaagaaaa aaaagtacaa ccaaatgcca
      781 gtcaggcaca gcagaaacct acaactcatg gaaggtaaag aacctgcaac tggagccaag
      841 aagagtaaca agccaaatga acagacaagt aaaagacatg acagtgatac tttcccagag
      901 ctgaagttaa caaatgcacc tggttctttt actaagtgtc caaataccag tgaacttaaa
      961 gaatttgtca atcctagcct tccaagagaa gaaaaagaag agaaactaga aacagttaaa
     1021 gtgtctaata atgctgaaga ccccaaagat ctcatgttaa gtggagaaag ggttttgcaa
     1081 actgaaagat ctgtagagag tagcagtatt tcactggtac ctggtactga ttatggcact
     1141 caggaaagta tctcgttact ggaagttagc actctaggga aggcaaaaac agaaccaaat
     1201 aaatgtgtga gtcagtgtgc agcatttgaa aaccccaagg gactaattca tggttgttcc
     1261 aaagataata gaaatgacac agaaggcttt aagtatccat tgggacatga agttaaccac
     1321 agtcgggaaa caagcataga aatggaagaa agtgaactcg atgctcagta tttgcagaat
     1381 acattcaagg tttcaaagcg ccagtcattt gctctgtttt caaatccagg aaatgcagaa
     1441 gaggaatgtg caacattctc tgcccactct gggtccttaa agaaacaaag tccaaaagtc
     1501 acttttgaat gtgaacaaaa ggaagaaaat caaggaaaga atgagtctaa tatcaagcct
     1561 gtacagacag ttaatatcac tgcaggcttt cctgtggttg gtcagaaaga taagccagtt
     1621 gataatgcca aatgtagtat caaaggaggc tctaggtttt gtctatcatc tcagttcaga
     1681 ggcaacgaaa ctggactcat tactccaaat aaacatggac ttttacaaaa cccatatcgt
     1741 ataccaccac tttttcccat caagtcattt gttaaaacta aatgtaagaa aaatctgcta
     1801 gaggaaaact ttgaggaaca ttcaatgtca cctgaaagag aaatgggaaa tgagaacatt
     1861 ccaagtacag tgagcacaat tagccgtaat aacattagag aaaatgtttt taaaggagcc
     1921 agctcaagca atattaatga agtaggttcc agtactaatg aagtgggctc cagtattaat
     1981 gaaataggtt ccagtgatga aaacattcaa gcagaactag gtagaaacag agggccaaaa
     2041 ttgaatgcta tgcttagatt aggggttttg caacctgagg tctataaaca aagtcttcct
     2101 ggaagtaatt gtaagcatcc tgaaataaaa aagcaagaat atgaagaagt agttcagact
     2161 gttaatacag atttctctcc atatctgatt tcagataact tagaacagcc tatgggaagt
     2221 agtcatgcat ctcaggtttg ttctgagaca cctgatgacc tgttagatga tggtgaaata
     2281 aaggaagata ctagttttgc tgaaaatgac attaaggaaa gttctgctgt ttttagcaaa
     2341 agcgtccaga gaggagagct tagcaggagt cctagccctt tcacccatac acatttggct
     2401 cagggttacc gaagaggggc caagaaatta gagtcctcag aagagaactt atctagtgag
     2461 gatgaagagc ttccctgctt ccaacacttg ttatttggta aagtaaacaa tataccttct
     2521 cagtctacta ggcatagcac cgttgctacc gagtgtctgt ctaagaacac agaggagaat
     2581 ttattatcat tgaagaatag cttaaatgac tgcagtaacc aggtaatatt ggcaaaggca
     2641 tctcaggaac atcaccttag tgaggaaaca aaatgttctg ctagcttgtt ttcttcacag
     2701 tgcagtgaat tggaagactt gactgcaaat acaaacaccc aggatccttt cttgattggt
     2761 tcttccaaac aaatgaggca tcagtctgaa agccagggag ttggtctgag tgacaaggaa
     2821 ttggtttcag atgatgaaga aagaggaacg ggcttggaag aaaataatca agaagagcaa
     2881 agcatggatt caaacttagg tgaagcagca tctgggtgtg agagtgaaac aagcgtctct
     2941 gaagactgct cagggctatc ctctcagagt gacattttaa ccactcagca gagggatacc
     3001 atgcaacata acctgataaa gctccagcag gaaatggctg aactagaagc tgtgttagaa
     3061 cagcatggga gccagccttc taacagctac ccttccatca taagtgactc ctctgccctt
     3121 gaggacctgc gaaatccaga acaaagcaca tcagaaaaag attcgcatat acatggccaa
     3181 agggacaact ccatgttttc taaaaggcct agagaacata tatcagtatt aacttcacag
     3241 aaaagtagtg aataccctat aagccagaat ccagaaggcc tttctgctga caagtttgag
     3301 gtgtctgcag atagttctac cagtaaaaat aaagaaccag gagtggaaag gtcatcccct
     3361 tctaaatgcc catcattaga tgataggtgg tacatgcaca gttgctctgg gagtcttcag
     3421 aatagaaact acccatctca agaggggctc attaaggttg ttgatgtgga ggagcaacag
     3481 ctggaagagt ctgggccaca cgatttgacg gaaacatctt acttgccaag gcaagatcta
     3541 gagggaaccc cttacctgga atctggaatc agcctcttct ctgatgaccc tgaatctgat
     3601 ccttctgaag acagagcccc agagtcagct cgtgttggca acataccatc ttcaacctct
     3661 gcattgaaag ttccccaatt gaaagttgca gaatctgccc agggtccagc tgctgctcat
     3721 actactgata ctgctgggta taatgcaatg gaagaaagtg tgagcaggga gaagccagaa
     3781 ttgacagctt caacagaaag ggtcaacaaa agaatgtcca tagtggtgtc tggcctgacc
     3841 ccagaagaat ttatgctcgt gtacaagttt gccagaaaac accacatcac tttaactaat
     3901 ctaattactg aagagactac tcatgttgtt atgaaaacag atgctgagtt tgtgtgtgaa
     3961 cggacactga aatattttct aggaattgcg ggaggaaaat gggtagttag ctatttctgg
     4021 gtgacccagt ctattaaaga aagaaaaatg ctgaatgagc atgattttga agtcagagga
     4081 gatgtggtca atggaagaaa ccaccaaggt ccaaagcgag caagagaatc ccaggacaga
     4141 aagatcttca gggggctaga aatctgttgc tatgggccct tcaccaacat gcccacagat
     4201 caactggaat ggatggtaca gctgtgtggt gcttctgtgg tgaaggagct ttcatcattc
     4261 acccttggca caggtgtcca cccaattgtg gttgtgcagc cagatgcctg gacagaggac
     4321 aatggcttcc atgcaattgg gcagatgtgt gaggcacctg tggtgacccg agagtgggtg
     4381 ttggacagtg tagcactcta ccagtgccag gagctggaca cctacctgat accccagatc
     4441 ccccacagcc actacttgcc aactttcttg tacaaagtgg tatacccata cgatgttcca
     4501 gattacgctt agtaatgaga aatactcaga agatgtcact caccatagtc cgcagtgaaa
     4561 gataaatgat cagaagacct tgataagcgc tgttttagag ctagaaatag caagtaggcc
     4621 actgagcaag aactatgaat gagatcttta gacatattgc ggccgctttc tccgcgatcc
     4681 agacatgata agatacattg atgagtttgg acaaaccaaa actagaatgc agtgaaaaaa
     4741 atgccttatt tgtgaaattt gtgatgctat tgccttattt gtaaccatta taagctgcaa
     4801 taaacaagtt atctataaca agaaaatata tatataataa gttatcacgt aagtagaaca
     4861 tgaaataaca atataattat cgtatgagtt aaatcttaaa agtcacgtaa aagataatca
     4921 tgcgtcattt tgactcacgc ggtcgttata gttcaaaatc agtgacactt accgcattga
     4981 caagcacgcc tcacgggagc tccaagcggc gactgagatg tcctaaatgc acagcgacgg
     5041 attcgcgcta tttagaaaga gagagcaata tttcaagaat gcatgcgtca attttacgca
     5101 gactatcttt ctagggttaa gaattcactg gccgtcgttt tacaacgtcg tgactgggaa
     5161 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     5221 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     5281 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     5341 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     5401 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     5461 gtgaccatgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacacg
     5521 cgagttcgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     5581 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     5641 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5701 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     5761 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5821 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5881 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5941 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     6001 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     6061 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     6121 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     6181 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     6241 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     6301 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     6361 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     6421 tctggagccg gtgagcgtgg ttcacgcggt atcattgcag cactggggcc agatggtaag
     6481 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     6541 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     6601 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     6661 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     6721 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6781 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6841 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6901 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6961 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     7021 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     7081 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     7141 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     7201 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     7261 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     7321 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     7381 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     7441 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     7501 gagtcagtga gcgaggaagc ggatgagcgc ccaatacgca aaccgcctct caccgcgcgt
     7561 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     7621 cgcaacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     7681 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     7741 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     7801 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag gtcgacttaa
     7861 ccctagaaag ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc
     7921 atgtgtttta tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt
     7981 tattatattt acacttacat actaataata aattcaacaa acaatttatt tatgtttatt
     8041 tatttattaa aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttagc
     8101 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     8161 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     8221 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
     8281 atgatcctct ggagattcat gcgccaggtt ttctggtcat gcaccacgtt cgtgggccct
     8341 caggaacctc cacgtcagcg gtaactgtga agcccagtcg ctcgtaaaaa ggaagattcc
     8401 ggggagcact ggtttcgagg aacgccggca cgccagcccg ttccgcggcc tcaacaccag
     8461 gaagaacgac agcgcttccc aaacctttac cttggtgatc tgggctgaca ccgacagtgg
     8521 caagaaacca cgcaggctcc tttggtctat gaggagccag caagccctcc atttgctgtt
     8581 gtgccgccaa cctactccct gacagctcgg ccatccgcgg acctatttcc gcgaacactg
     8641 cgccggcttc gacgctttcc ggagtagtcc agaccgcaac tgcggcacca tcgtccgcaa
     8701 cccaaacttt accaatgtcc agacccacgc gcgtaagaaa cagctcttgc aactcagtta
     8761 ccctctctat gtgtctgtcc ggatctacgg tatggcgggt cgctggataa tctgcaaatg
     8821 ctgcggcaag tgttcggaca gcccgtggta catcgtcacg ggtagcgagc ctaacagtgg
     8881 gcttatattc cgtcatgggg ccggggttct cctccacgtc gccggcctgc ttcagcaggc
     8941 tgaagttggt ggcgccgctg cccccgggga gcatgtcaag gtcaaaatcg tcaagagcgt
     9001 cagcaggcag catatcaagg tcaaagtcgt caagggcatc ggctgggagc atgtctaagt
     9061 caaaatcgtc aagggcgtcg gtcggcccgc cgctttcgca ctttagctgt ttctccaggc
     9121 cacatatgat tagttccagg ccgaaaagga aggcaggttc ggctccctgc cggtcgaaca
     9181 gctcaattgc ttgtttcaga agtgggggca tagaatcggt ggtaggtgtc tctctttcct
     9241 cttttgctac ttgatgctcc tgttcctcca atacgcagcc cagtgtaaag tggcccacgg
     9301 cggacagagc gtacagtgcg ttctccaggg agaagccttg ctgacacagg aacgcgagct
     9361 gattttccag ggtttcgtac tgtttctctg ttgggcgggt gccgagatgc actttagccc
     9421 cgtcacgatg tgagaggaga gcacagcggt atgacttggc gttgttccgc agaaagtctt
     9481 gccatgactc gccttccagg gggcaggagt gggtatgatg cctgtccagc atctcgattg
     9541 gcagggcatc gagcagggcc cgcttgttct tcacgtgcca gtacagggta ggctgctcaa
     9601 ctcccagctt ttgagcgagt ttccttgtcg tcaggccttc gataccgact ccattgagta
     9661 attccagagc agagtttatg actttgctct tgtccagtct agacatctta tcgtcatcgt
     9721 ctttgtaatc catggtggcg gatcccgcgt cacgacacct gtgttctggc ggcaaacccg
     9781 ttgcgaaaaa gaacgttcac ggcgactact gcacttatat acggttctcc cccaccctcg
     9841 ggaaaaaggc ggagccagta cacgacatca ctttcccagt ttaccccgcg ccaccttctc
     9901 taggcaccgg ttcaattgcc gacccctccc cccaacttct cgggcactgt gggccatgtg
     9961 cgctctgccc catggtaata gccatgacta atacgtagat gtactgccaa gtaggaaagt
    10021 gccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa
    10081 tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta
    10141 aatactccac ccattgacgt caatggaaag tgcctattgg cgttactatg ggaacatacg
    10201 tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg
    10261 taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt
    10321 actattaata actagtcaat aatcaatgtc ataagctcta atcgcataat cgagctaaga
    10381 gcgattaata tgcttagcta gcttagctaa gctagatttc tctattctcc cacaatatat
    10441 agcagctatt agcttagcag tccgaatacg atcgtccacg atccataagc gtaacacatt
    10501 tgagtagcga ctgacgggca ccggagccac tcgagtggaa tta
//
