LOCUS       sequence-44255-e         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Wang JW, Beck ES, McCabe BD
  TITLE     A modular toolset for recombination transgenesis and neurogenetic 
            analysis of Drosophila.
  JOURNAL   PLoS One. 2012;7(7):e42102. Epub 2012 Jul 25.
  PUBMED    22848718
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(7)"; pages: "e42102. Epub 2012 Jul 25"
COMMENT     Sequence Label: pBID-UASC-MRG
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(41..130)
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     protein_bind    373..467
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    491..585
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
ORIGIN
        1 tgttctcctt aatcagctcc tcgcccttgc tcactctaga cagatcttcc tcagagatga
       61 gcttctgttc cagatcttct tctgaaatca acttttgttc cagatcttct tcagagatga
      121 gtttctgctc catgttgata agcttggcca gctggtcgac ctgcagatcc ggccctcgag
      181 ccgcggccgc agatctgcga attcgtttgg tatgcgtctt gtgattcaaa gttggcttat
      241 tcaaaggata ttaacgaagg cagcggcacg tctgctcggc tcgaacattc attcacaact
      301 gatgctctca gccaccccgc gcccttttat accgctgcgc tcgatccccg ggcgagctcg
      361 ctagagtctc cgctcggagg acagtactcc gctcggagga cagtactccg ctcggaggac
      421 agtactccgc tcggaggaca gtactccgct cggaggacag tactccgacc tgcaggcatg
      481 caagcttccg ctcggaggac agtactccgc tcggaggaca gtactccgct cggaggacag
      541 tactccgctc ggaggacagt actccgctcg gaggacagta ctccgacctg caggcatgca
      601 agcggatcga gccgcggcca gcttggatct gttgttggtt ggcacaccac aaatatactg
      661 ttgccgagca caattgatcg gctaaatggt atggcaagaa aaggtatgca atataataat
      721 cttttattgg gtatgcaacg aaaatttgtt tcgtcaacgt atgcaatatt ttttattaaa
      781 a
//
