LOCUS       sequence-442563-        4632 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes L-subunit and M-subunit of bacterial photosynthetic reaction
            center in R. sphaeroides.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4632)
  TITLE     Reaction center amber suppression plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTZ18U-pufLM-Clonable
FEATURES             Location/Qualifiers
     source          1..4632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     235..254
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        235..253
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2088..2105)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2088..2104)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2097..2119)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2246..2701
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2333..2352)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2543..2564
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2727..2831
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2832..2900,2901..3692)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3050..3069)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3863..4451
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4352..4371
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4605..4622
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg aatttaatac
      241 gactcactat agggaattcg agctcggtac ccggggatcc gttgggccga ctgcaagcgg
      301 agagggaagc atggcactgc tcagcttcga gcgaaaatat cgcgtgccgg ggggcacgct
      361 ggtcggcgga aacctgttcg acttctgggt cggccctttc tatgtcggct tcttcggggt
      421 tgcgacgttt ttcttcgcgg ccctgggtat cattctgatt gcctggagtg ccgtactcca
      481 gggtacctgg aacccccaac tcatctctgt ctacccgccg gcccttgaat atggcctggg
      541 aggtgcaccc ctcgcaaaag gcgggctgtg gcagatcatc acgatctgcg ccactggtgc
      601 cttcgtcagc tgggcgctgc gcgaagtcga aatctgccgt aagctgggca tcgggtacca
      661 catcccgttc gccttcgcgt tcgccatcct ggcctacctg acgctggtgc tgttccgccc
      721 ggtgatgatg ggcgcctggg gctatgcctt cccctacggg atctggacgc acctcgactg
      781 ggtgtcgaac acgggctaca cctacggcaa cttccactac aaccctgccc acatgatcgc
      841 catctcgttc ttcttcacga acgcgctggc tctggcgctg cacggcgccc ttgtgctctc
      901 cgcggccaac cccgagaagg gcaaggaaat gcggacgccg gatcacgagg atacgttctt
      961 ccgcgatctg gtcggctact cgatcgggac gctcggcatc caccgcctcg gcctgctgct
     1021 ctcgctgagc gccgtcttct tcagcgccct ctgcatgatc attaccggca ccatctggtt
     1081 cgatcagtgg gtcgactggt ggcaatggtg ggtgaagctg ccgtggtggg cgaacatccc
     1141 gggaggcatc aatggctgag tatcagaaca tcttctccca ggtccaggtc cgcggaccgg
     1201 ccgacctggg gatgaccgaa gacgtcaacc tggccaaccg ttcgggcgtc ggtcccttct
     1261 cgaccctgct cggctggttc ggcaacgccc agctcggccc gatctatctc ggctcgctcg
     1321 gcgtcctgtc cctcttctcg ggcctgatgt ggttcttcac catcgggatc tggttctggt
     1381 atcaggcggg ctggaacccg gccgtcttcc tgcgcgacct gttcttcttc tcgctcgagc
     1441 cgccggcacc cgaatacggt ctgtccttcg cggctccgct gaaggaaggc gggctgtggc
     1501 tgatcgcgtc gttcttcatg ttcgtcgcgg tctggtcctg gtggggccgc acctatctcc
     1561 gcgctcaggc gctgggcatg ggcaagcaca ccgcctgggc gttcctctcg gccatctggc
     1621 tgtggatggt gctgggcttc atccgtccga tcctcatggg gtcctggtcg gaagcggttc
     1681 cctacggcat cttctcgcac ctcgactgga cgaacaactt ctcgctcgtc cacggcaacc
     1741 tgttctacaa ccccttccac ggtctctcga tcgccttcct ctacgggtcg gccctgctct
     1801 tcgcgatgca cggtgcgacc atcctcgcgg tctcccgctt cggcggcgag cgcgagctgg
     1861 agcagatcgc cgaccgcggg acggcagcgg agcgggccgc cctcttctgg cgctggacca
     1921 tgggtttcaa cgccacgatg gaaggcatcc accgctgggc catctggatg gcggtcctcg
     1981 tgaccctcac cggcggcatc ggcatcctgc tctcgggcac ggtcgtggac aactggtacg
     2041 tctggggcca gaaccacggc atggcgccgc tgaactgaac atatggcact ggccgtcgtt
     2101 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     2161 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     2221 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     2281 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     2341 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     2401 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     2461 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     2521 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     2581 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     2641 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     2701 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     2761 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     2821 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     2881 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     2941 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     3001 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     3061 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     3121 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     3181 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     3241 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     3301 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     3361 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     3421 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     3481 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     3541 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     3601 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     3661 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     3721 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     3781 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     3841 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     3901 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     3961 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     4021 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     4081 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4141 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4201 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     4261 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     4321 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     4381 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     4441 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     4501 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     4561 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     4621 aggaagcgga ag
//
