LOCUS       sequence-442565-       11373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses sfGFP and aaRS-tRNA pair for incorporating haloalkane 
            crosslinker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11373)
  TITLE     Reaction center amber suppression plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pIND4-GFP-MmXYRS
FEATURES             Location/Qualifiers
     source          1..11373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      485..571
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(742..1557)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1465..1484
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1549..1568
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      5329..5917
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5818..5837
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6071..6088
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(6426..7508)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     7470..7489
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(7509..7586)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     primer_bind     7778..7796
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    7842..7858
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    7875..7891
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             7910..7921
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(7928..7930,7931..7933,7934..8644)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             8651..8668
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      8680..8774
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(8884..8903)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     11000..11022
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11014..11031
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11015..11031
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt
       61 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc
      121 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata
      181 tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca
      241 atatggacaa cttcttcgcc cccgttttca ccatgcatgg gcaaatatta tacgcaaggc
      301 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat
      361 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa
      421 tttttttaag gcagttattg gtgcccttaa acgcctgggg taatgactct ctagcttgag
      481 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      541 gtcggtgaac gctctcctga gtaggacaaa tccgccgcta ggagcttgcg gccctggcgt
      601 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
      661 tgcgatttat tcaacaaagc cgccgtcccg tcaagtcagc gtaatgctct gccagtgtta
      721 caaccaatta accaattctg attagaaaaa ctcatcgagc atcaaatgaa actgcaattt
      781 attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga
      841 aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac
      901 tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga
      961 gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aacagcttat gcatttcttt
     1021 ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa
     1081 accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg
     1141 acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat
     1201 attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc
     1261 agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg
     1321 cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct
     1381 acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat
     1441 tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc
     1501 catgttggaa tttaatcgcg gcttcgagca agacgtttcc cgttgaatat ggctcataac
     1561 accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt
     1621 atcttgtgca atgtaacatc agagattttg agacacaacg tggctttgtt gaataaatcg
     1681 aacttttgct gagttgaagg atcagatcac gcatcttccc gacaacgcag accgttccgt
     1741 ggcaaagcaa aagttcaaaa tcaccaactg gtccacctac aacaaagctc tcatcaaccg
     1801 tggctccctc gaggctccgc gccctcgatc cgaccgcccc ccacaaggct ggggcggatc
     1861 gggggcgcac ggcctgaagg gtgcagggga accctgccgc acgcaaactc gcagagtttg
     1921 catagtgtgc gccattcaga taccttcatg gcaggcccca ccgtggattt tgctgtcatg
     1981 gttcgattca tcacgacgcc cgcaacgccg actgcacagc ggcccgcacc acgaacgcct
     2041 gcgcagcagg aagctgcggc tgaactgaag gctgcgcgaa ctgccgaagc agcccgccgc
     2101 ctcgacaccc ggcgcaaggt ggtgctcggg ggcgcgctcc tggcactggc agaacgtgat
     2161 gcagcagcag ccgcgatgct ggcccggctg aagcagatgc tgacccgccc agcagatcgg
     2221 aagctgttcg cggatctacc cgatggctga cggtgcaatc ttccacctcg atgtgcgcac
     2281 ggtcagccgg tcagatggcc gctcggctgt cgccgctgcc gcctatcggt cggccagccg
     2341 tctgcacgat gcacgcaccg gcctgaccca cgacttcgcg cgcaagcggg gcgtcttgac
     2401 aaccttcatc gccgccccgg acggctgcga ctggatcaca gacagggaca cgctctggaa
     2461 cgcggctgag gcggcagaga agcgcaagaa cagcaccgtg gcccgtgagt ggctggtggc
     2521 cctccctgac gctctggatg ctacacagcg ggccgacctg gcgcgggcgc tggcggtcga
     2581 actggcgcag cggttcgggg tggccgtgga tgtggcaatc cacgcagctt cgggcgaggg
     2641 cgaccagcgg aaccatcacg cgcatttgct gaccacgacg cgcaccgcag gcccggacgg
     2701 gctgggcgac aagacccgcg ttctggatgc agccaaaacg gggggcgcag aaatctacgc
     2761 catgcgggct tggtgggcgg gcacggtcaa cgatgcactc gcggccgcac aaagctcggc
     2821 ccgcgtcgat caccgccgca tagccgtgat cgccgccgat gcgcgggccg aggccgaggc
     2881 gctggagaaa caggccaaag ccgtcgaaac cctgaacgcc aagcccgccg acgccggggg
     2941 gctgtggaag ggccttggca gcgccgcccg cgcaatatgg gaaggcggca tgaccgccct
     3001 caaatcgacc gccgagacag ccaacgagct gcgggagaag gccaacgagc tgcgacagaa
     3061 ggcagcccgc tacacccgcg ccaccgaacc tcaccacggc cccgtcctga cggccttctt
     3121 gcggcgcatg gctcccgtct gggcggagca ggccaaggcc gaggaagccc ggagagccgc
     3181 gcaggaggcc gcacagcgcg cactgatggc cgagagggct gaacaggccc ggaaggccaa
     3241 ggaagcctct gaggccgctc gccgggccgc tacggcagcg cgagaggccg aggaagcccg
     3301 cgaacgctgc gcccgcgagg tgttgccgct catccgccgc gcccggcacg acccgatcac
     3361 cgccgaagtg atcaagcggg agggcatcga cctcgaccgc cccgaccaac aggtggcccg
     3421 cgacccgatc tggctcatgg ccggacgcca agatcaggcg cacccggtct ggctgatcgt
     3481 ccacgcctac accgccgcgc gcgacaggca cgcgaacctg ctggcgagaa tcgaagccaa
     3541 aaccgcagcc cagatcgccg ctgccgccaa accaaagccc aagcctgcca gtcccgcccc
     3601 gaccgaaccc gaacccgatc cagacccacc gccccggcgg gattggggcg ggccgtctgg
     3661 accctgatcc accccccatt ctcagcaact ccgagccagc cagaggcgct ggctcggggc
     3721 atctggaccc ccttcctcac cccaaatttt cacgaaaaag gggcgcagcc cttcttgttc
     3781 aataagttct ataagttcag gcgaaaatcg cctttcattt tcaatggctt gcgcgcctat
     3841 cggggagaaa tggggccgca aaagggagaa atggggccgc aaaagggaga aatggggccg
     3901 atagcggctg ttgacagggg gatattttgc cccctacatt tctccccatg acaaagacca
     3961 cgcaaatcgc cgctgatcga gcctatgacg aggccaaaac cgtgctgccc gcagaggtgg
     4021 cgcggggggt ctacatgcgc aacgccccca gcctcgccgc cctcaagctg atgcacctgc
     4081 tgatcggtaa ggcgggcggg cgtatggccg aacccattcg gcacgaaatc cgcctctcag
     4141 acatccgcaa gatcgacggg atgaagaacc atgacagggc cagcctcacc ccccttttcg
     4201 cggaattgtc ggcggctgtc ctgacccatg atgaccccga aaaaatggtc gtgaccattg
     4261 gcggcttgct ggacttcgcc cgcatcgact accgccacga agtcagcaac gatctgctgg
     4321 tgtcgtggta ttttggcggg gccttccgcg agatggccga gaagtcctgc cactgggcga
     4381 tcctcgaccg gcagaccgtt tttgccctgt ccagcaagta ttcgatcctg ctgttccagc
     4441 acatcgccag cctcgtgaac ctcgatcacg tcgccagcaa gaccttcacc gtggccgaac
     4501 tgcgggccgt gctaggggtg ccgcacggaa agatggagcg gtggaacgac gtgaaccgct
     4561 ttgcgctgac gcccgcgatt gccgagatca accagacctc gcgcctgatc ctgaccgcga
     4621 ccccgaacaa gatcgggcgg acggtcgcca gcgtgacaat cacgtgggcg gagaagcctc
     4681 ccgagggcaa gcaggaagcc aagcgcgaac tggaccgccc caaggtgggc cgagaggcgc
     4741 ggcgcaacgg ctcagccgag accgtggcgc gggccttccc cagctcgggc agcatcgagt
     4801 tcgatgacca ctggaaggcc ctcaagcggg cggcgggctg caacatggac aacaccatga
     4861 ttgccgagaa gttccgggcg tggtgcgcca gcaaggggct ttccctcgac ggccgaaaca
     4921 tcgagcaggc gttcagcaac ttctgcgcca cagtgggcaa ggtctgaggc cgcgcccgct
     4981 gccagtgacc cgccgccgcc gaccgtgagc gggccgcagc ccttccagcg ccgcccaaga
     5041 gggcggcaag ccccggtctg ccccctcgtc gcccgcaaag cgggcagggg gcagaccggg
     5101 gcgctggaac ctttgggctg ctttgacggc cagacagccc ccgccgcgca agcgcgccat
     5161 tcccccaccc gtggccgggc gggggaatgg taaagcatgt gggggcagtc cagccgtcaa
     5221 tccgcgctcc gcaccacggc gcctcgagca acaccttctt cacgaggcag acctctcgac
     5281 ggagttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     5341 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     5401 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     5461 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     5521 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     5581 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     5641 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     5701 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     5761 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     5821 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     5881 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     5941 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     6001 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     6061 gaccgagcgc agcgagtcag tgagcgagga agcggaagaa gctcgcacat tcagcagcgt
     6121 ttttcagcgc gttttcgatc aacgtttcaa tgttggtatc aacaccaggt ttaactttga
     6181 acttatcggc actgacggtt actgattttg aacttttgct ttgccacgga acggtctgcg
     6241 ttgtcgggaa gatgcgtgat ctgatccttc aactcagcaa aagttcgcca atacgcaaac
     6301 cgcctctccc cgcgcgttgg ccgattcatt aatgcagggc cgcgcgaatt cgagctcggt
     6361 accgacgtag cccagcgcgt cggccagctt gcaattcgcg ctaacttaca ttaattgcgt
     6421 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     6481 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc
     6541 agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag
     6601 cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg
     6661 atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg
     6721 cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc
     6781 agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac
     6841 atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat
     6901 ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc
     6961 gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca
     7021 tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga
     7081 acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg
     7141 atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg
     7201 ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta
     7261 atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc
     7321 agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc
     7381 gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc
     7441 acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact
     7501 ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga
     7561 aaggttttgc accattcgat ggtgtcaacg taaatgcatg ccgcttcgcc ttcgcgcgcg
     7621 aattgcaggt accatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     7681 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     7741 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     7801 tcgtcttcac ctcgagaaaa tttatcaaaa agagtgttga cttgtgagcg gataacaatg
     7861 atacttagat tcaattgtga gcggataaca atttcacaca tctagaatta aagaggagaa
     7921 attaaccatg gttagcaaag gtgaagaact gtttaccggc gttgtgccga ttctggtgga
     7981 actggatggt gatgtgaatg gccataaatt tagcgttcgt ggcgaaggcg aaggtgatgc
     8041 gaccaacggt aaactgaccc tgaaatttat ttgcaccacc ggtaaactgc cggttccgtg
     8101 gccgaccctg gtgaccaccc tgacctatgg cgttcagtgc tttagccgct atccggatca
     8161 tatgaaacgc catgatttct ttaaaagcgc gatgccggaa ggctatgtgc aggaacgtac
     8221 cattagcttc aaagatgatg gcacctataa aacccgtgcg gaagttaaat ttgaaggcga
     8281 taccctggtg aaccgcattg aactgaaagg tattgatttt aaagaagatg gcaacattct
     8341 gggtcataaa ctggaatata atttcaacag ccataatgtg tatattaccg ccgataaaca
     8401 gaaaaatggc atcaaagcga actttaaaat ccgtcacaac gtggaagatg gtagcgtgca
     8461 gctggcggat cattatcagc agaatacccc gattggtgat ggcccggtgc tgctgccgga
     8521 taatcattat ctgagcaccc agagcgttct gagcaaagat ccgaatgaaa aacgtgatca
     8581 tatggtgctg ctggaatttg ttaccgccgc gggcattacc cacggtatgg atgaactgta
     8641 taaaggcagc caccatcatc atcaccatta atgagcttgg actcctgttg atagatccag
     8701 taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt
     8761 ttattggtga gaatccaagc tagcttggcg agattttcag gagctaagga agctaaaatg
     8821 gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat
     8881 tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt
     8941 acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac
     9001 attcttgccc gcctgatgaa tgctcatgct agcgagctcg ccgctcatgg cgttctgttg
     9061 cccgtctcac tggtgaaaag aaaaacaacc ctggcgccgc ttctttgagc gaacgatcaa
     9121 aaataagtgg cgccccatca aattgttacg gagcccaaaa aatccgcttg cgcccggggc
     9181 cgtctgctcc tagaaaccgc ttcaccgaga cgaagaccgg cagcgccgga cggagacgag
     9241 ggagcggatg acagaaacgt cggccgcgac aattgaagat gaggcggacg ggatcgctgg
     9301 ttgtctgcat caacggaggt cccccatgga taagaaaccc ctcaacacgc tgatctccgc
     9361 gacgggcctc tggatgagcc ggacgggcac gatccacaag atcaaacatc atgaggtgtc
     9421 ccggtccaag atctatatcg aaatggcctg cggcgatcat ctggtggtga ataattcccg
     9481 gtccagccgc acggcccggg cgctgcggca tcataagtat cgcaagacgt gcaaacgctg
     9541 ccgggtcagc gatgaagacc tgaacaagtt cctcaccaag gccaatgagg accagacctc
     9601 ggtcaaagtg aaggtggtct ccgcacccac ccgtacgaag aaggccatgc ccaaatcggt
     9661 cgcccgtgcg ccgaagcccc tcgaaaacac ggaagcagcg caggcgcagc cgtcgggcag
     9721 caagttctcg cccgccatcc ccgtgtcgac gcaggaaagc gtgagcgtgc ccgcgtcggt
     9781 ctcgacctcc atctcctcca tctccaccgg ggcgacggcg agcgccctgg tgaaaggcaa
     9841 cacgaatccg atcacgtcga tgtcggcgcc cgtccaggcc agcgcgccgg cgctcaccaa
     9901 gagccagacc gatcgcctgg aggtgctcct caatcccaaa gatgaaatct ccctcaattc
     9961 cgggaagccg ttccgcgaac tggaatccga gctgctctcc cggcggaaaa ggatctgcag
    10021 cagatctacg ccgaagaacg ggagaactat ctgggcaagc tggagcgcga aatcacgcgg
    10081 ttcttcgtgg accgcggctt cctcgaaatc aagagcccga tcctcatccc gctcgaatat
    10141 atcgaacgga tgggcatcga caatgacacg gagctctcca aacagatctt ccgggtggac
    10201 aagaatttct gcctccgccc catgctcacc cccaacctct acaactacct gcgcaagctc
    10261 gaccgcgcgc tccccgatcc catcaaaatc ttcgaaatcg gcccctgcta tcgcaaggag
    10321 tccgacggca aggaacacct cgaagaattc acgatgctcg cgttcgcgca gatggggtcc
    10381 gggtgcacgc gggaaaacct ggagagcatc atcacggact tcctcaatca cctgggcatc
    10441 gatttcaaga tcgtgggcga ctcgtgcatg gtcttcggcg acaccctcga tgtgatgcat
    10501 ggggacctgg aactctcctc cgcggtcgtg gggcccatcc cgctcgaccg ggagtggggc
    10561 atcgacaagc ccaccatcgg cgcggggttc ggcctcgaac gcctcctcaa ggtcaagcac
    10621 gacttcaaaa atatcaagcg ggccgcccgc tccgagtcct actacaatgg catcagcacc
    10681 aacctgtaag atccggtgga tgaccttttg aatgaccttt aatagattat attactaatt
    10741 aattggggac cctagaggtc ccctttttta ttttaaaaat tttttcacaa aacggtttac
    10801 aagcataaag cttgctcaat caatcaccca tcagccctga ggtcgggctc cttgcatgca
    10861 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
    10921 caggagtcgc ataagggaga gcgtctggcg aaagggggat gtgctgcaag gcgattaagt
    10981 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctt
    11041 aaaaaaaatc cttagctttc gctaaggtgg cggaaacccc gggaatctaa cccggctgaa
    11101 cggatttaga gtccattcga tctacatgat caggtttccc gttacacaga caaccagcga
    11161 tcccgtccgc ctcatcttca attgtcgcgg ccgacgtttc tgtcatccgc tccctcgtct
    11221 ccgtccggcg ctgccggtct tcgtctcggt gaagcggttt ctaggagcag acggccccgg
    11281 gcgcaagcgg attttttggg ctccgtaaca attttcactc tcgagtccgg aagtactatt
    11341 tcgaatttcg tatggcaatg aaagacggtg agc
//
