LOCUS       sequence-442610-        9970 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of human RNF8 (S157A) in mammalian cells .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9970)
  AUTHORS   Chuah YH, Tay EXY, Grinchuk OV, Yoon J, Feng J, Kannan S, Robert M, 
            Jakhar R, Liang Y, Lee BWL, Wang LC, Lim YT, Zhao T, Sobota RM, Lu 
            G, Low BC, Crasta KC, Verma CS, Lin Z, Ong DST
  TITLE     CAMK2D serves as a molecular scaffold for RNF8-MAD2 complex to 
            induce mitotic checkpoint in glioma.
  JOURNAL   Cell Death Differ. 2023 Aug;30(8):1973-1987. doi: 
            10.1038/s41418-023-01192-3. Epub 2023 Jul 19.
  PUBMED    37468549
REFERENCE   2  (bases 1 to 9970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41418-023-01192-3"; journalName: "Cell Death Differ"; 
            date: "2023-08"; volume: "30"; issue: "8"; pages: "1973-1987"
COMMENT     Sequence Label: pHAGE-RNF8-S157A
FEATURES             Location/Qualifiers
     source          1..9970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(6..8,9..11,12..725)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6..8,9..11,12..722)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(51..72)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(312..331)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     659..680
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    739..1327
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(792..812)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1210..1221)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1402..1635
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(1754..1772)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1840..1944
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1945..2013,2014..2805)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2163..2182)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2976..3564
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3465..3484
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3718..3735
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3975..3996
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4011..4016,4017..4034,4035..4041)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4049..4065
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4054..4076
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4073..4089
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4073..4089
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4139..4158
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             4350..4983
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5030..5155
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5652..5885
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6070..6114
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6407..6524
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6572..7750
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6803..7741
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7698..7718
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    7770..7794
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9256..9280)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9333..9374
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    9395..9962
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    9412..9964
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9556..9573)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9783..9802
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 cacatatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
       61 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
      121 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
      181 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
      241 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
      301 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
      361 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
      421 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
      481 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
      541 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
      601 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
      661 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
      721 agtaaatcga tagatcctaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
      781 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
      841 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
      901 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
      961 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     1021 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     1081 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     1141 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     1201 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     1261 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     1321 tccccgcctg agatccttta agaccaatga cttacaaggc agctgtagat cttagccact
     1381 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     1441 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     1501 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     1561 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     1621 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     1681 agaaatgaat atcagagagt gagaggcccg ggttaattaa ggaaagggct agatcattct
     1741 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     1801 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     1861 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     1921 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     1981 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     2041 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     2101 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     2161 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     2221 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     2281 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     2341 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     2401 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     2461 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     2521 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     2581 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     2641 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     2701 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     2761 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     2821 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     2881 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     2941 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     3001 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     3061 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     3121 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     3181 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     3241 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3301 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3361 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3421 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3481 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3541 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3601 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3661 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3721 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     3781 tggccgattc attaatgcag caagctcatg gctgactaat tttttttatt tatgcagagg
     3841 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     3901 taggcttttg caaaaagctc cccgtggcac gacaggtttc ccgactggaa agcgggcagt
     3961 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     4021 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     4081 agctatgaca tgattacgaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4141 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct
     4201 aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg
     4261 tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt
     4321 tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc aaagaagaca
     4381 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta
     4441 cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc
     4501 agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc
     4561 tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag
     4621 ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg
     4681 atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg
     4741 cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta
     4801 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     4861 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     4921 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     4981 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     5041 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     5101 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     5161 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     5221 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     5281 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     5341 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     5401 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     5461 gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt cagacctgga
     5521 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     5581 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     5641 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     5701 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     5761 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     5821 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     5881 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     5941 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     6001 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     6061 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     6121 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     6181 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     6241 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
     6301 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
     6361 tctcgacggt atcgccgaat tcacaaatgg cagtattcat ccacaatttt aaaagaaaag
     6421 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     6481 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     6541 acagcagaga tccagtttgg actagtcgtg aggctccggt gcccgtcagt gggcagagcg
     6601 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta
     6661 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
     6721 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
     6781 cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt
     6841 tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt acgtgattct
     6901 tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc
     6961 cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat
     7021 ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt
     7081 ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga
     7141 tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca
     7201 gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta
     7261 gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc
     7321 ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc
     7381 cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga
     7441 gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac
     7501 ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga gtacgtcgtc
     7561 tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac
     7621 tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt
     7681 tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca
     7741 ggtgtcgtga agcggccctg cagatatcaa caagtttgta caaaaaagca ggcaccatgg
     7801 gggagcccgg cttcttcgtc acaggagacc gcgccggtgg ccggagctgg tgcctgcggc
     7861 gggtggggat gagcgccggg tggctgctgc tggaagatgg gtgcgaggtg actgtaggac
     7921 gaggatttgg tgtcacatac caactggtat caaaaatctg ccccctgatg atttctcgaa
     7981 accactgtgt tttgaagcag aatcctgagg gccaatggac aattatggac aacaagagtc
     8041 taaatggtgt ttggctgaac agagcgcgtc tggaaccttt aagggtctat tccattcatc
     8101 agggagacta catccaactt ggagtgcctc tggaaaataa ggagaatgcg gagtatgaat
     8161 atgaagttac tgaagaagac tgggagacaa tatatccttg tctttcccca aagaatgacc
     8221 aaatgataga aaaaaataag gaattgagaa ctaaaaggaa attcgctttg gatgaattag
     8281 caggtcctgg agctgaaggc ccctcaaatt tgaaatccaa aataaataaa gtgtcttgtg
     8341 aatctggtca gccagtgaaa tcacagggga aaggtgaagt ggccagtaca ccctctgaca
     8401 atttggatcc taagttgact gcccttgagc caagtaagac cacaggggct cccatttacc
     8461 ctggcttccc caaagtcaca gaggttcatc atgagcagaa agcctcaaac tcttcagcat
     8521 ctcagagaag cttacagatg tttaaggtga ccatgtccag gattctgagg ctcaaaatac
     8581 agatgcagga aaaacatgaa gccgttatga atgtgaaaaa gcagacccaa aaggggaact
     8641 caaagaaagt tgtgcaaatg gagcaggaac ttcaggactt acagtcccag ctgtgtgcag
     8701 agcaggctca gcagcaggca agagtggagc aactagagaa gactttccag gaagaggaac
     8761 agcatcttca gggtttggag atagcccaag gagaaaagga cctgaagcaa cagctggccc
     8821 aggctctgca ggagcattgg gctctaatgg aagagctaaa tcgcagcaag aaggactttg
     8881 aagcaatcat tcaagccaag aacaaagaat tagagcagac caaggaagag aaggagaaga
     8941 tgcaagcaca gaaggaagaa gttcttagcc acatgaatga tgtgctagag aatgagctcc
     9001 aatgtattat ttgttcagaa tacttcattg aggctgtcac cttgaactgt gcccacagtt
     9061 tctgctccta ctgtatcaat gaatggatga agcggaagat agaatgcccc atttgtcgga
     9121 aggacattaa gtccaaaaca tactctttgg ttctggacaa ttgcattaat aagatggtaa
     9181 ataatctgag ctcagaagtg aaagaacgac gaattgttct cattagggaa cgaaaagcaa
     9241 agagattgtt ctagaaccca gctttcttgt acaaagtggt tgatatccag cacagtggcg
     9301 gccgctcgag tctagagggc ccgcggttcg aaggtaagcc tatccctaac cctctcctcg
     9361 gtctcgattc tacgcgtacc ggttagtaat gagatccctc ccccccccct aacgttactg
     9421 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     9481 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     9541 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     9601 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     9661 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     9721 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     9781 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     9841 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa
     9901 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat
     9961 aatatggcca
//
