LOCUS       sequence-442612-        9970 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of human RNF8 (R42A) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9970)
  AUTHORS   Chuah YH, Tay EXY, Grinchuk OV, Yoon J, Feng J, Kannan S, Robert M, 
            Jakhar R, Liang Y, Lee BWL, Wang LC, Lim YT, Zhao T, Sobota RM, Lu 
            G, Low BC, Crasta KC, Verma CS, Lin Z, Ong DST
  TITLE     CAMK2D serves as a molecular scaffold for RNF8-MAD2 complex to 
            induce mitotic checkpoint in glioma.
  JOURNAL   Cell Death Differ. 2023 Aug;30(8):1973-1987. doi: 
            10.1038/s41418-023-01192-3. Epub 2023 Jul 19.
  PUBMED    37468549
REFERENCE   2  (bases 1 to 9970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41418-023-01192-3"; journalName: "Cell Death Differ"; 
            date: "2023-08"; volume: "30"; issue: "8"; pages: "1973-1987"
COMMENT     Sequence Label: pHAGE-RNF8-R42A
FEATURES             Location/Qualifiers
     source          1..9970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(320..364)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    549..782
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1279..1404
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1451..2084
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2276..2295)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2345..2361)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2345..2361)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2358..2380)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2369..2385
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2393..2399,2400..2417,2418..2423))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2438..2459
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2699..2716)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2870..3458)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2950..2969)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3629..4420,4421..4489))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4252..4271
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4490..4594)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4662..4680
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             4799..5032
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    5107..5695
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5213..5224
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5622..5642
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(5709..6422,6423..6425,6426..6428))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(5712..6422,6423..6425,6426..6428))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5754..5775)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6103..6122
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6362..6383
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    6440..6992
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6442..7009
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6602..6621)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     6831..6848
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(7030..7071)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    7124..7148
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8610..8634)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(8654..9832)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          8663..9601
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(8686..8706)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    join(9880..9970,1..27)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccccaatcc ccccttttct tttaaaattg tggatgaata ctgccatttg tgaattcggc
       61 gataccgtcg agatccgttc actaatcgaa tggatctgtc tctgtctctc tctccacctt
      121 cttcttctat tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg gtctgaaacg
      181 ataatggtga atatccctgc ctaactctat tcactataga aagtacagca aaaactattc
      241 ttaaacctac caagcctcct actatcatta tgaataattt tatataccac agccaatttg
      301 ttatgttaaa ccaattccac aaacttgccc atttatctaa ttccaataat tcttgttcat
      361 tcttttcttg ctggttttgc gattcttcaa ttaaggagtg tattaagctt gtgtaattgt
      421 taatttctct gtcccactcc atccaggtcg tgtgattcca aatctgttcc agagatttat
      481 tactccaact agcattccaa ggcacagcag tggtgcaaat gagttttcca gagcaacccc
      541 aaatccccag gagctgttga tcctttaggt atctttccac agccaggatt cttgcctgga
      601 gctgcttgat gccccagact gtgagttgca acagatgctg ttgcgcctca atagccctca
      661 gcaaattgtt ctgctgctgc actataccag acaataattg tctggcctgt accgtcagcg
      721 tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa gaacccaagg aacaaagctc
      781 ctattcccac tgctcttttt tctctctgca ccactcttct ctttgccttg gtgggtgcta
      841 ctcctaatgg ttcaattttt actactttat atttatataa ttcacttctc caattgtccc
      901 tcatatctcc tcctccaggt ctgaagatca gcggccggcc gcttgctgtg cggtggtctt
      961 acttttgttt tgctcttcct ctatcttgtc taaagcttcc ttggtgtctt ttatctctat
     1021 cctttgatgc acacaataga gggttgctac tgtattatat aatgatctaa gttcttctga
     1081 tcctgtctga agggatggtt gtagctgtcc cagtatttgt ctacagcctt ctgatgtttc
     1141 taacaggcca ggattaactg cgaatcgttc tagctccctg cttgcccata ctatatgttt
     1201 taatttatat tttttctttc cccctggcct taaccgaatt ttttcccatc gcgatctaat
     1261 tctcccccgc ttaatactga cgctctcgca cccatctctc tccttctagc ctccgctagt
     1321 caaaattttt ggcgtactca ccagtcgccg cccctcgcct cttgccgtgc gcgcttcagc
     1381 aagccgagtc ctgcgtcgag agagctcctc tggtttccct ttcgctttca agtccctgtt
     1441 cgggcgccac tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta
     1501 ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga
     1561 ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac
     1621 cagagagacc cagtacaggc aaaaagcagc tgcttatatg caggatctga gggctcgcca
     1681 ctccccagtc ccgcccaggc cacgcctccc tggaaagtcc ccagcggaaa gtcccttgta
     1741 gcaagctcga tatcagcagt tcttgaagta ctccggatgc agctctcggg ccacgtgatg
     1801 aaatgctagg cggctgtcaa acctccactc taacacttct ctctccgggt catccatccc
     1861 atgcaggctc acagggtgta acaagctggt gttctctcct ttattggcct cttctacctt
     1921 atctggctca actggtacta gcttgtagca ccatccaaag gtcagtggat atctgacccc
     1981 tggccctggt gtgtagttct gctaatcagg gaagtagcct tgtgtgtggt agatccacag
     2041 atcaaggata tcttgtcttc tttgggagtg aattagccct tccaactact aagtttgtag
     2101 tacatattta acaaatacaa tttctttaaa atgaaaataa ttcagaggaa tcacaggttt
     2161 agagtaaatg aaaccacagg taattggcag tggtaatagg gtatggggtg ggaagtttgg
     2221 gatgattttg gttagcttga gttatccagt tgatccagac atgataagat acattgatga
     2281 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaattcgtaa
     2341 tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     2401 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     2461 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccac ggggagcttt
     2521 ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc
     2581 gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgagc ttgctgcatt
     2641 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     2701 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     2761 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     2821 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     2881 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     2941 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3001 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3061 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     3121 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     3181 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     3241 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     3301 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     3361 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     3421 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     3481 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     3541 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     3601 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     3661 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     3721 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     3781 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     3841 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     3901 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     3961 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     4021 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     4081 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     4141 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     4201 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
     4261 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     4321 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     4381 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     4441 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     4501 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     4561 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     4621 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     4681 cctttcgtct tcaagaatga tctagccctt tccttaatta acccgggcct ctcactctct
     4741 gatattcatt tctttgcaag ttataaatac tgaataataa gatgacatga actactactg
     4801 ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac
     4861 aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt
     4921 gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca
     4981 gtacaagcaa aaagcagatc ttgtcttcgt tgggagtgaa ttagcccttc cagtcccccc
     5041 ttttctttta aaaagtggct aagatctaca gctgccttgt aagtcattgg tcttaaagga
     5101 tctcaggcgg ggaggcggcc caaagggaga tccgactcgt ctgagggcga aggcgaagac
     5161 gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg aaggtccgct ggattgaggg
     5221 ccgaagggac gtagcagaag gacgtcccgc gcagaatcca ggtggcaaca caggcgagca
     5281 gtcaaggaaa ggacgatgat ttccccgaca acaccacgga attgtcagtg cccaacagcc
     5341 gagcccctgt ccagcagcgg gcaaggcagg cggcgatgag ttccgccgtg gcaataggga
     5401 gggggaaagc gaaagtcccg gaaaggagct gacaggtggt ggcaatgccc caaccagtgg
     5461 gggttgcgtc agcaaacaca gtgcacacca cgccacgttg cctgacaacg ggccacaact
     5521 cctcataaag agacagcaac caggatttat acaaggagga gaaaatgaaa gccatacggg
     5581 aagcaatagc atgatacaaa ggcattaaag cagcgtatcc acatagcgta aaaggagcaa
     5641 catagttaag aataccagtc aatctttcac aaattttgta atccagaggt tgattaggat
     5701 ctatcgattt acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac
     5761 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggta
     5821 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
     5881 tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc
     5941 ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac
     6001 tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg
     6061 cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg
     6121 tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag
     6181 tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg
     6241 gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc
     6301 agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt
     6361 acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg
     6421 ctcaccatat gtgtggccat attatcatcg tgtttttcaa aggaaaacca cgtccccgtg
     6481 gttcgggggg cctagacgtt tttttaacct cgactaaaca catgtaaagc atgtgcaccg
     6541 aggccccaga tcagatccca tacaatgggg taccttctgg gcatccttca gccccttgtt
     6601 gaatacgctt gaggagagcc atttgactct ttccacaact atccaactca caacgtggca
     6661 ctggggttgt gccgcctttg caggtgtatc ttatacacgt ggcttttggc cgcagaggca
     6721 cctgtcgcca ggtggggggt tccgctgcct gcaaagggtc gctacagacg ttgtttgtct
     6781 tcaagaagct tccagaggaa ctgcttcctt cacgacattc aacagacctt gcattccttt
     6841 ggcgagaggg gaaagacccc taggaatgct cgtcaagaag acagggccag gtttccgggc
     6901 cctcacattg ccaaaagacg gcaatatggt ggaaaataac atatagacaa acgcacaccg
     6961 gccttattcc aagcggcttc ggccagtaac gttagggggg ggggagggat ctcattacta
     7021 accggtacgc gtagaatcga gaccgaggag agggttaggg ataggcttac cttcgaaccg
     7081 cgggccctct agactcgagc ggccgccact gtgctggata tcaaccactt tgtacaagaa
     7141 agctgggttc tagaacaatc tctttgcttt tcgttcccta atgagaacaa ttcgtcgttc
     7201 tttcacttct gagctcagat tatttaccat cttattaatg caattgtcca gaaccaaaga
     7261 gtatgttttg gacttaatgt ccttccgaca aatggggcat tctatcttcc gcttcatcca
     7321 ttcattgata cagtaggagc agaaactgtg ggcacagttc aaggtgacag cctcaatgaa
     7381 gtattctgaa caaataatac attggagctc attctctagc acatcattca tgtggctaag
     7441 aacttcttcc ttctgtgctt gcatcttctc cttctcttcc ttggtctgct ctaattcttt
     7501 gttcttggct tgaatgattg cttcaaagtc cttcttgctg cgatttagct cttccattag
     7561 agcccaatgc tcctgcagag cctgggccag ctgttgcttc aggtcctttt ctccttgggc
     7621 tatctccaaa ccctgaagat gctgttcctc ttcctggaaa gtcttctcta gttgctccac
     7681 tcttgcctgc tgctgagcct gctctgcaca cagctgggac tgtaagtcct gaagttcctg
     7741 ctccatttgc acaactttct ttgagttccc cttttgggtc tgctttttca cattcataac
     7801 ggcttcatgt ttttcctgca tctgtatttt gagcctcaga atcctggaca tggtcacctt
     7861 aaacatctgt aagcttctct gagatgctga agagtttgag gctttctgct catgatgaac
     7921 ctctgtgact ttggggaagc cagggtaaat gggagcccct gtggtcttac ttggctcaag
     7981 ggcagtcaac ttaggatcca aattgtcaga gggtgtactg gccacttcac ctttcccctg
     8041 tgatttcact ggctgaccag attcacaaga cactttattt attttggatt tcaaatttga
     8101 ggggccttca gctccaggac ctgctaattc atccaaactg aatttccttt tagttctcaa
     8161 ttccttattt ttttctatca tttggtcatt ctttggggaa agacaaggat atattgtctc
     8221 ccagtcttct tcagtaactt catattcata ctccgcattc tccttatttt ccagaggcac
     8281 tccaagttgg atgtagtctc cctgatgaat ggaatagacc cttaaaggtt ccagacgcgc
     8341 tctgttcagc caaacaccat ttagactctt gttgtccata attgtccatt ggccctcagg
     8401 attctgcttc aaaacacagt ggtttcgaga aatcatcagg gggcagattt ttgataccag
     8461 ttggtatgtg acaccaaatc ctgctcctac agtcacctcg cacccatctt ccagcagcag
     8521 ccacccggcg ctcatcccca cccgccgcag gcaccagctc cggccaccgg cgcggtctcc
     8581 tgtgacgaag aagccgggct cccccatggt gcctgctttt ttgtacaaac ttgttgatat
     8641 ctgcagggcc gcttcacgac acctgaaatg gaagaaaaaa actttgaacc actgtctgag
     8701 gcttgagaat gaaccaagat ccaaactcaa aaagggcaaa ttccaaggag aattacatca
     8761 agtgccaagc tggcctaact tcagtctcca cccactcagt gtggggaaac tccatcgcat
     8821 aaaacccctc cccccaacct aaagacgacg tactccaaaa gctcgagaac taatcgaggt
     8881 gcctggacgg cgcccggtac tccgtggagt cacatgaagc gacggctgag gacggaaagg
     8941 cccttttcct ttgtgtgggt gactcacccg cccgctctcc cgagcgccgc gtcctccatt
     9001 ttgagctccc tgcagcaggg ccgggaagcg gccatctttc cgctcacgca actggtgccg
     9061 accgggccag ccttgccgcc cagggcgggg cgatacacgg cggcgcgagg ccaggcacca
     9121 gagcaggccg gccagcttga gactaccccc gtccgattct cggtggccgc gctcgcaggc
     9181 cccgcctcgc cgaacatgtg cgctgggacg cacgggcccc gtcgccgccc gcggccccaa
     9241 aaaccgaaat accagtgtgc agatcttggc ccgcatttac aagactatct tgccagaaaa
     9301 aaagcgtcgc agcaggtcat caaaaatttt aaatggctag agacttatcg aaagcagcga
     9361 gacaggcgcg aaggtgccac cagattcgca cgcggcggcc ccagcgccca ggccaggcct
     9421 caactcaagc acgaggcgaa ggggctcctt aagcgcaagg cctcgaactc tcccacccac
     9481 ttccaacccg aagctcggga tcaagaatca cgtactgcag ccaggtggaa gtaattcaag
     9541 gcacgcaagg gccataaccc gtaaagaggc caggcccgcg ggaaccacac acggcactta
     9601 cctgtgttct ggcggcaaac ccgttgcgaa aaagaacgtt cacggcgact actgcactta
     9661 tatacggttc tcccccaccc tcgggaaaaa ggcggagcca gtacacgaca tcactttccc
     9721 agtttacccc gcgccacctt ctctaggcac cggttcaatt gccgacccct ccccccaact
     9781 tctcggggac tgtgggcgat gtgcgctctg cccactgacg ggcaccggag cctcacgact
     9841 agtccaaact ggatctctgc tgtccctgta ataaacccga aaattttgaa tttttgtaat
     9901 ttgtttttgt aattctttag tttgtatgtc tgttgctatt atgtctacta ttctttcccc
     9961 tgcactgtac
//
