LOCUS       sequence-442616-        6581 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  unc-25 fluorescent neural reporter driving nuclear mTagBFP2 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6581)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 6581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY25
FEATURES             Location/Qualifiers
     source          1..6581
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1946..1966
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1988..2008
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4172..4194)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4294..4313
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4391..4409)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4477..4581
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4582..4650,4651..5442)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4800..4819)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5613..6201
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6102..6121
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6355..6372
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6489..6510
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6525..6530,6531..6548,6549..6555)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6563..6579
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(6568..6581,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagc gcacgtcttc
      181 ttattcttct tcttcttctt cttttcctac cttttctatc ccactttttc cccggaattt
      241 tgagatttga catgattttt cagagaattt cgagttttga gaaaaaaaaa atcaaaaaag
      301 cgattttttg gtcaaaagcc gaaatttaaa gctagttttt ttggaaatat cgattttttt
      361 tttgagaatt tttcgatttt ccaaaaaaaa aatcgaaagt tgtttcccga aaagccgaaa
      421 ttttttttga gatatcgaaa aatcggagaa tttttcgatg tttcccagaa atactaaaat
      481 tttgaatttt ccggaaaaaa aatcgttaaa aaattagttt cttttaaaga ccgaaattta
      541 aagttttttt tttggaaata tcgatttttt ccgagatatc taaaattgaa agaaattttc
      601 gattttctga gaaaaatacg aaaaaatcga aaaaaaaacc caaatttcgg ttttttcgta
      661 aaaaaagtcg taaaaatgta atttttttcc tgaaaaatcg gaatttttta caaaatatcg
      721 gaaaatactc aaaaaaaagc tgaaaatttc gattttccgg agaaaaaatc tttttaaaaa
      781 aatatttttt ttttcagaaa ataagaaaag ccgaaattta taactatttc tccggaaatt
      841 cgaaattttt aacgaaatat cggaataatt tttagatttt tcaagtttga ctttgcgaga
      901 aaaaaatcgg aaaaacctcg attcgacgcc gaaaaatgct ccttttcgaa aagatttttg
      961 aaatttcaga aaatcgacat gcaagcgcgc tccacggcga aatgacaacg atgatccacc
     1021 gccctcaaaa agttgggtct cgttaggtat ttggcggtaa aactggtaaa actccagttt
     1081 tgcctccaac gagacccaat tttttggggc ggtggtggag cgcgcttgca caagctgaaa
     1141 gcatttttct gcgactcgat aatattttga aaacctgtgt caattctcga aattcttttt
     1201 ttaaaaaata atcccgagct tctctcagtc ctcctctatg aggatgttcc ttttttttgg
     1261 tttttcaatt ttttttaaaa ttccaaattt ctgttgtgca attcacttcc ccccaagaaa
     1321 tcccaaaaat ccccagtttt ccccaaaaat gttccgtttt catgtgattt ttcccccatt
     1381 tttaaaacat ttttttgact tttttttaaa atgattatta ttattgtttt ctatttcatg
     1441 gccggtaaat tatttttttt ctttcttttt ttttgctctt ttttttcaag aattttcgaa
     1501 ttgtttgaag ggctgctcat ctaatctttt gtcattttgt tctgatgcca tcatttctga
     1561 gaggaccttt gaagactcgt cacgaaacgg gaggggggct caagtgagca ttattattat
     1621 tattattgtc gcaaaaagtt taccccgggc tccccctggc tcccctcttt gagcaagggt
     1681 ttaagggctc attttgatga cgaattgctc attgggatta tagtcacgcc cctcttttgg
     1741 agcaactaca caactgagcc acagtaatcc ttgggggcgg ggtcagtagg accccctccg
     1801 gaatagggaa aagctcagtt caccgccaaa acccgggatt ggccaaagga cccaaaggta
     1861 tgtttcgaat gatactaaca taacatagaa cattttcagg aggacccttg cttggagggt
     1921 accgagctca gaaaaaatga ccgctccaaa gaagaagcgc aaggttccag tcgagaagat
     1981 gactgcccca aaaaagaaac gtaaggtccc gcggatgtca gagcttatta aggagaatat
     2041 gcatatgaaa ctgtacatgg aaggaaccgt ggacaaccat cactttaagt gcacttcgga
     2101 gggggaaggc aaaccgtacg agggcaccca gaccatgcga atcaaggtgg tcgagggagg
     2161 cccactgccc ttcgccttcg acattctggt aagtttaaac atatatatac taactaaccc
     2221 tgattattta aattttcagg ctactagctt cctgtacggc agcaagactt ttattaatca
     2281 cacccagggc atcccggact tcttcaaaca gtcgttccct gagggcttca catgggagcg
     2341 tgtgaccaca tacgaagacg gtggcgttct caccgctacc caggacacca gcctccagga
     2401 cggttgcctc atatataatg tggtaagttt aaacagttcg gtactaacta accatacata
     2461 tttaaatttt cagaagatcc ggggagtaaa cttcacatcc aatggtccgg ttatgcagaa
     2521 gaaaacactc ggctgggagg ccttcaccga gacgctgtat ccggctgacg gaggcctgga
     2581 aggtcgaaac gacatggccc tgaagctcgt cggcggtagc catctcatcg caaacgctaa
     2641 gaccacatat agatccaaga aaccggtaag tttaaacatg attttactaa ctaactaatc
     2701 tgatttaaat tttcaggcta agaaccttaa aatgccggga gtctactatg ttgactacag
     2761 actggaacgg atcaaggagg ccaacaacga gacctacgtc gagcagcacg aggttgcagt
     2821 tgccagatac tgcgacctcc cgagcaaact gggtcacaag cttaatccta ggataccggt
     2881 gggtgaagac ccaccaaagc catctgccaa gggagccaag aaggccgcca agaccgttac
     2941 gaagccaaag gacggaaaga agagacgtca tgcccgtaag gaatcatact ccgtctacat
     3001 ctaccgtgtc ctcaagcaag ttcatccaga cactggagtt tcctccaaag ccatgtctat
     3061 catgaactct tttgtcaacg atgtcttcga gcgtattgct gctgaagcat cccgtcttgc
     3121 tcactacaac aagcgttcca caatctcatc ccgcgaaatt cagaccgctg tccgtctgat
     3181 ccttccagga gagcttgcca agcacgccgt gtctgaggga accaaggccg ttaccaagta
     3241 cacttccagc aagtaggaat tccaactgag cgccggtcgc taccattacc aacttgtctg
     3301 gtgtcaaaaa taataggggc cgctgtcatc agagtaagtt taaactgagt tctactaact
     3361 aacgagtaat atttaaattt tcagcatctc gcgcccgtgc ctctgacttc taagtccaat
     3421 tactcttcaa catccctaca tgctctttct ccctgtgctc ccacccccta tttttgttat
     3481 tatcaaaaaa cttctcttaa tttctttgtt ttttagcttc ttttaagtca cctctaacaa
     3541 tgaaattgtg tagattcaaa aatagaatta attcgtaata aaaagtcgaa aaaaattgtg
     3601 ctccctcccc ccattaataa taattctatc ccaaaatcta cacaatgttc tgtgtacact
     3661 tcttatgttt tttacttctg ataaattttt ttgaaacatc atagaaaaaa ccgcacacaa
     3721 aataccttat catatgttac gtttcagttt atgaccgcaa tttttatttc ttcgcacgtc
     3781 tgggcctctc atgacgtcaa atcatgctca tcgtgaaaaa gttttggagt atttttggaa
     3841 tttttcaatc aagtgaaagt ttatgaaatt aattttcctg cttttgcttt ttggggtttc
     3901 ccctattgtt tgtcaagatt tcgaggacgg cgtttttctt gctaaaatca caagtattga
     3961 tgagcacgat gcaagaaaga tcggaagaag gtttgggttt gaggctcagt ggaaggtgag
     4021 tagaagttga taatttgaaa gtggagtagt gtctatgggg tttttgcctt aaatgacaga
     4081 atacattccc aatataccaa acataactgt ttcctactag tcggccgtac gggccctttc
     4141 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     4201 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     4261 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     4321 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     4381 ggccttaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     4441 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4501 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4561 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4621 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     4681 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4741 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     4801 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4861 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4921 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     4981 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     5041 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     5101 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     5161 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     5221 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     5281 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     5341 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     5401 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     5461 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     5521 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5581 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5641 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     5701 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     5761 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5821 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5881 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5941 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6001 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6061 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     6121 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     6181 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     6241 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     6301 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     6361 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     6421 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     6481 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     6541 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat t
//
