LOCUS       sequence-442618-        6635 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  osm-9 fluorescent neural reporter driving nuclear CyOFP1 expression 
            (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 6635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY34
FEATURES             Location/Qualifiers
     source          1..6635
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..21
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5..21
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1993..2013
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2035..2055
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4225..4247)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4347..4366
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4444..4462)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4530..4634
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4635..4703,4704..5495)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4853..4872)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5666..6254
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6155..6174
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6408..6425
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6542..6563
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6578..6583,6584..6601,6602..6608)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6616..6632
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(6621..6635,1..8)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 cacacaggaa acagctatga ccatgattac gccaagctgt aagtttaaac atgatcttac
       61 taactaacta ttctcattta aattttcaga gcttaaaaat ggctgaaatc actcacaacg
      121 atggatacgc taacaacttg gaaatgaaat aagcttgcat gcctgcagca ctagaaggac
      181 gttgcgcaac ctgaattcct cgcgggagta ctttacgggt aactaggtat attttgaaac
      241 ttgctagtta gcctaaaaaa cagtgagcgc ccctttttta aaaaggtttt tgagaatcag
      301 tactctggca attcaacttt tatcgagatc gtattttctc acttctttac ccaaaaaaag
      361 caatacctag aatatagcaa tggagcccat ttcatatgtg ggatttcgaa aatttccgaa
      421 ctttcctgtg aaaaattaat ataggtaacg gattcagcat gtcaaaaaac taatgtagat
      481 attattccat tagcctacct gaaaatttga gatatcagta ggaaactatt ggaataaatc
      541 cggctacaaa atatttcaac atttttattc tttagataag tttcaaattg gacttgtatc
      601 tcgaaatgta tccaactaat caataaaatt ctccactcac gtttttttct ttttcaaaat
      661 tctcagaact agaacatgct ctagcactct cactgcactt gctttcaatc aattttcaga
      721 gcaggtcttg tcaggtcact aagattattg atttaagtgg ggttgctctt atgaaatata
      781 gtattgcttt gagttgtagt cttatagact ttaatagttt tttttgcaaa tttttgtata
      841 cataatattg attaatggac attctagaat taattgacgt gtttattagt tcagtcacaa
      901 ttttttttga aaactttagc acatttggtg tatagaccca aatgccaaat gccaaatatt
      961 atgcactttt aaagtttggg acacattata agagacgaca ctcgttcctt acccttaact
     1021 tcataagcac ctataactct gcactgattg tgagtaaatt tatcaaaaaa attgcacgtt
     1081 ttaaacgtga aggttagctt tttgttagtt gacaattttt tgataacacc taccgcaaag
     1141 gagttatgcg cctttaaagt atgggacaca aagtgagtga gcagcgcttc cttcttcacc
     1201 caatattctg tgagttgagt tgtcaaaaag ttggcaacta acaaaatgtt taaaattttg
     1261 agataaataa tgtgtaagca cacaactgct tgatcacact taccgctgca agaatgcttt
     1321 gagaaaatta acgaaatttt caaaattttg aatatcgtaa aattatttca tgtactaaaa
     1381 actagttgtt taccttttat gttcatccgg actgtgcttt agagttgaag caaaaagttc
     1441 aatttattgt cttaactcaa cccgtttcct tccatcttgc tcggcgccgc ctgaaggtgt
     1501 gtctcatggg gtgtgactaa tccacgtcgt caattgcgaa aatatacgct ttttcacttc
     1561 cattactaca cacacacact tccgctagcc aaattgtggg acccaaaaag tatgacatga
     1621 tcctttaaag caggtggaaa ttattcccgt gttctcagaa tttctttctc tctctctgat
     1681 tttgttctga tcaaaatata attttcttat cttcatattc atactcattt atagtattat
     1741 tcgaaagtat aaaatttgtt cttcacctcc aaatttcata taacttttgt attactaaga
     1801 aggcggagtt ggctctcgct gtctatatta tcaatttttc accaactctt taattttcca
     1861 attttttcag aaaccaaacc cgggattggc caaaggaccc aaaggtatgt ttcgaatgat
     1921 actaacataa catagaacat tttcaggagg acccttgctt ggagggtacc gagctcagaa
     1981 aaaatgaccg ctccaaagaa gaagcgcaag gttccagtcg agaagatgac tgccccaaaa
     2041 aagaaacgta aggtcccgcg ggtaagttta aacagttcgg tactaactaa ccatacatat
     2101 ttaaattttc agcgcgctgt gtcaaaagga gaagagctta ttaaggagaa tatgcgttcc
     2161 aagctctacc tcgagggatc cgtcaacgga caccaattca agtgcaccca cgagggagag
     2221 ggaaagccat acgagggaaa gcaaaccaac cgtatcaagg tcgtcgaggg aggaccactc
     2281 ccattcgcct tcgacatcct cgccacccac ttcatgtacg gatccaaggt aagtttaaac
     2341 atatatatac taactaaccc tgattattta aattttcagg tcttcatcaa gtacccagcc
     2401 gacctcccag actacttcaa gcaatccttc ccagagggat tcacctggga gcgtgtcatg
     2461 gtcttcgagg acggaggagt cctcaccgcc acccaagaca cctccctcca agacggagag
     2521 ctcatctaca acgtcaaggt ccgtggagtc aacttcccag ccaacggacc agtcatgcaa
     2581 aagaagaccc tcggatggga gccatccacc gagaccatgt acccagccga cggaggactc
     2641 gagggacgtt gcgacaaggt aagtttaaac atgattttac taactaacta atctgattta
     2701 aattttcagg ccctcaagct cgtcggagga ggacacctcc acgtcaactt caagaccacc
     2761 tacaagtcca agaagccagt caagatgcca ggagtccact acgtcgaccg tcgtctcgag
     2821 cgtatcaagg aggccgacaa cgagacctac gtcgagcaat acgagcacgc cgtcgcccgt
     2881 tactccaacc tcggaggagg aatggacgag ctctacaagc ctaggatacc ggtgggtgaa
     2941 gacccaccaa agccatctgc caagggagcc aagaaggccg ccaagaccgt tacgaagcca
     3001 aaggacggaa agaagagacg tcatgcccgt aaggaatcat actccgtcta catctaccgt
     3061 gtcctcaagc aagttcatcc agacactgga gtttcctcca aagccatgtc tatcatgaac
     3121 tcttttgtca acgatgtctt cgagcgtatt gctgctgaag catcccgtct tgctcactac
     3181 aacaagcgtt ccacaatctc atcccgcgaa attcagaccg ctgtccgtct gatccttcca
     3241 ggagagcttg ccaagcacgc cgtgtctgag ggaaccaagg ccgttaccaa gtacacttcc
     3301 agcaagtagg aattccaact gagcgccggt cgctaccatt accaacttgt ctggtgtcaa
     3361 aaataatagg ggccgctgtc atcagagtaa gtttaaactg agttctacta actaacgagt
     3421 aatatttaaa ttttcagcat ctcgcgcccg tgcctctgac ttctaagtcc aattactctt
     3481 caacatccct acatgctctt tctccctgtg ctcccacccc ctatttttgt tattatcaaa
     3541 aaacttctct taatttcttt gttttttagc ttcttttaag tcacctctaa caatgaaatt
     3601 gtgtagattc aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt gtgctccctc
     3661 cccccattaa taataattct atcccaaaat ctacacaatg ttctgtgtac acttcttatg
     3721 ttttttactt ctgataaatt tttttgaaac atcatagaaa aaaccgcaca caaaatacct
     3781 tatcatatgt tacgtttcag tttatgaccg caatttttat ttcttcgcac gtctgggcct
     3841 ctcatgacgt caaatcatgc tcatcgtgaa aaagttttgg agtatttttg gaatttttca
     3901 atcaagtgaa agtttatgaa attaattttc ctgcttttgc tttttggggt ttcccctatt
     3961 gtttgtcaag atttcgagga cggcgttttt cttgctaaaa tcacaagtat tgatgagcac
     4021 gatgcaagaa agatcggaag aaggtttggg tttgaggctc agtggaaggt gagtagaagt
     4081 tgataatttg aaagtggagt agtgtctatg gggtttttgc cttaaatgac agaatacatt
     4141 cccaatatac caaacataac tgtttcctac tagtcggccg tacgggccct ttcgtctcgc
     4201 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     4261 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     4321 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     4381 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcggcctta
     4441 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     4501 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4561 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4621 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     4681 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     4741 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     4801 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     4861 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     4921 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     4981 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     5041 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     5101 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     5161 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     5221 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     5281 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     5341 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     5401 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     5461 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     5521 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     5581 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     5641 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     5701 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     5761 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     5821 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     5881 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     5941 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6001 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     6061 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6121 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     6181 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     6241 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     6301 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     6361 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     6421 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     6481 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     6541 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     6601 gtatgttgtg tggaattgtg agcggataac aattt
//
