LOCUS       sequence-442620-        7327 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  sra-6 fluorescent neural reporter driving nuclear mTagBFP2 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7327)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 7327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY121
FEATURES             Location/Qualifiers
     source          1..7327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2692..2712
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2734..2754
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4918..4940)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5040..5059
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5137..5155)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5223..5327
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5328..5396,5397..6188)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5546..5565)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6359..6947
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6848..6867
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7101..7118
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7235..7256
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7271..7276,7277..7294,7295..7301)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7309..7325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(7314..7327,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcaga tctgaggtgc
      181 atttgcgagg ggcacttcag atttgaaact ttgaattttg cacctcgcca tgtctataaa
      241 aggaggagtt tgataatatc tgacgcaagt agagtacaat tttattaatc caaataaatt
      301 gtgccaattt ttataagatg atataaatga gaaacgggaa ttgagaatat gatgagtagt
      361 ttttaaagtt tgtcattcaa aattgttgtt gcctgccatg caaatgaaag tcagaagagt
      421 tccaaaccag aagaggaaat atgatttttc catgtaacag tagttgtctt tttttagaac
      481 ggcaggccaa acacaaaagt attccattat ttcctgattt gccttttcta aacaatttcc
      541 agtcaagtga taaattcaca tttccagtgc tctgaaaatc ttgtcaaaaa gtttgtattt
      601 cggatgtcag gtgatgtttg taagacatac gttagctggt ctcttacgta ttccaaaacc
      661 gctacaatat gataatcagt caaaatgaaa cactaaagtt tttgaactga atgatcacag
      721 agttgattga gatagataga gttataattt tgaaggaatc ttacgcatac ggaagcttca
      781 aagagtttag gataatttca tagttagagt agacgtgaca aagttattaa tatcataatt
      841 tgtttttctc gaaattgaat atgactgaaa cgcaagtcag agagattcct gaacctaata
      901 atctaaaatt ttaattgcta ttttcaatat tgcatttttc gattcacgcg taaaggaaga
      961 gacaatttca cagcatcgga actatcttcc gatttggaag ataatttttt aaagtgtttt
     1021 ccttattggc tttgaaattt caataaaagt acacctgacg aattttcaaa taagtgaaaa
     1081 atagaagcag acatcgagta aacgtgcata aaataaatgc aataagtgct cgttaagtaa
     1141 tgaaataaag ttagtctaca aattgatcaa ccagtacatg actaaaaatt ggtgactgtt
     1201 ttgttttagt ctttacccaa tttttatttc agcgatttca ttttatactc aaatgtgatt
     1261 tttctacatt ttttcgaatt tagaaaagat cagacgattt ccttaaagag gatttctagt
     1321 ttagtctttg attcaataac ttatagtaca gcttagttat aactatagtt cactatgaaa
     1381 tatcacattt ataatcgttt catttaattg taaggacatt tttgcatcaa acgccagttt
     1441 tctgtcccca tttaattata agttttactc ggccgatgcg agatggatgc tcctctcaca
     1501 caccttaccc cacgccttga caccttattt attgggtttt aaattttttt gatattttag
     1561 agaattataa aggtttccgt taaaaaaaaa catggccttc aaggcgagta cgtctgcgtt
     1621 gtaaactcac aaataaactt aaaaaatatt tttttttgga ttgaagcttt atttgaaagg
     1681 ataactcact cgctatctaa aaattgagaa agttttgtaa taacacgaaa tttcatgaag
     1741 tgcggaaaat ttttgaaaat tggatgaaac tgttcctttc acgagtttgg tcgtgtttta
     1801 ttcttcattc aaataccaat gcaacagtcg gttttaactt ttcaaaatta tacttcaagt
     1861 ttttacaagt gcatatgtaa aagtgttttc tatcttcaca ccttcgaaaa cagcaataat
     1921 aaattaaagc cgcgtgcagc tatcttatag tttggttctc tgtattttca aatatcttaa
     1981 gtgtcacatc aaattcaatg ataatttgga aatcacagaa gatctttaat gtgtttatag
     2041 tctctatgaa ctcatcaatt acaaatatat ttctagtcag actattcaca caaaccgcag
     2101 tttttaatca tccatatctt cttctcatgg aagttctagc taacgtcaag tcagactttc
     2161 acaagtatga gttttatttt gtccaacatt ttatgctctt gatgaactca aatatcattt
     2221 ctctttaaac tatagtttca taagattcag taattttgat tctgcagttc agattctaaa
     2281 tttatccgaa gagaaaacca attgatcaag tctttattca atttccgaga aaacgtggtt
     2341 gagcttaaca cattttagaa tttgtaaacg tgttgttgtt tttcaaaacc tcgctaataa
     2401 tttctcccta gttactcccg taaagtattt tctggaaaat ttgcaggttt aatagagttg
     2461 gtttgaaata ctgcaaatca aaacactttt tatagattaa atcaattttg ataatgtttt
     2521 ctgtcacaca taataaactg cactcgcaga atttaatatt tattatttca gattttgccc
     2581 gggattggcc aaaggaccca aaggtatgtt tcgaatgata ctaacataac atagaacatt
     2641 ttcaggagga cccttgcttg gagggtaccg agctcagaaa aaatgaccgc tccaaagaag
     2701 aagcgcaagg ttccagtcga gaagatgact gccccaaaaa agaaacgtaa ggtcccgcgg
     2761 atgtcagagc ttattaagga gaatatgcat atgaaactgt acatggaagg aaccgtggac
     2821 aaccatcact ttaagtgcac ttcggagggg gaaggcaaac cgtacgaggg cacccagacc
     2881 atgcgaatca aggtggtcga gggaggccca ctgcccttcg ccttcgacat tctggtaagt
     2941 ttaaacatat atatactaac taaccctgat tatttaaatt ttcaggctac tagcttcctg
     3001 tacggcagca agacttttat taatcacacc cagggcatcc cggacttctt caaacagtcg
     3061 ttccctgagg gcttcacatg ggagcgtgtg accacatacg aagacggtgg cgttctcacc
     3121 gctacccagg acaccagcct ccaggacggt tgcctcatat ataatgtggt aagtttaaac
     3181 agttcggtac taactaacca tacatattta aattttcaga agatccgggg agtaaacttc
     3241 acatccaatg gtccggttat gcagaagaaa acactcggct gggaggcctt caccgagacg
     3301 ctgtatccgg ctgacggagg cctggaaggt cgaaacgaca tggccctgaa gctcgtcggc
     3361 ggtagccatc tcatcgcaaa cgctaagacc acatatagat ccaagaaacc ggtaagttta
     3421 aacatgattt tactaactaa ctaatctgat ttaaattttc aggctaagaa ccttaaaatg
     3481 ccgggagtct actatgttga ctacagactg gaacggatca aggaggccaa caacgagacc
     3541 tacgtcgagc agcacgaggt tgcagttgcc agatactgcg acctcccgag caaactgggt
     3601 cacaagctta atcctaggat accggtgggt gaagacccac caaagccatc tgccaaggga
     3661 gccaagaagg ccgccaagac cgttacgaag ccaaaggacg gaaagaagag acgtcatgcc
     3721 cgtaaggaat catactccgt ctacatctac cgtgtcctca agcaagttca tccagacact
     3781 ggagtttcct ccaaagccat gtctatcatg aactcttttg tcaacgatgt cttcgagcgt
     3841 attgctgctg aagcatcccg tcttgctcac tacaacaagc gttccacaat ctcatcccgc
     3901 gaaattcaga ccgctgtccg tctgatcctt ccaggagagc ttgccaagca cgccgtgtct
     3961 gagggaacca aggccgttac caagtacact tccagcaagt aggaattcca actgagcgcc
     4021 ggtcgctacc attaccaact tgtctggtgt caaaaataat aggggccgct gtcatcagag
     4081 taagtttaaa ctgagttcta ctaactaacg agtaatattt aaattttcag catctcgcgc
     4141 ccgtgcctct gacttctaag tccaattact cttcaacatc cctacatgct ctttctccct
     4201 gtgctcccac cccctatttt tgttattatc aaaaaacttc tcttaatttc tttgtttttt
     4261 agcttctttt aagtcacctc taacaatgaa attgtgtaga ttcaaaaata gaattaattc
     4321 gtaataaaaa gtcgaaaaaa attgtgctcc ctccccccat taataataat tctatcccaa
     4381 aatctacaca atgttctgtg tacacttctt atgtttttta cttctgataa atttttttga
     4441 aacatcatag aaaaaaccgc acacaaaata ccttatcata tgttacgttt cagtttatga
     4501 ccgcaatttt tatttcttcg cacgtctggg cctctcatga cgtcaaatca tgctcatcgt
     4561 gaaaaagttt tggagtattt ttggaatttt tcaatcaagt gaaagtttat gaaattaatt
     4621 ttcctgcttt tgctttttgg ggtttcccct attgtttgtc aagatttcga ggacggcgtt
     4681 tttcttgcta aaatcacaag tattgatgag cacgatgcaa gaaagatcgg aagaaggttt
     4741 gggtttgagg ctcagtggaa ggtgagtaga agttgataat ttgaaagtgg agtagtgtct
     4801 atggggtttt tgccttaaat gacagaatac attcccaata taccaaacat aactgtttcc
     4861 tactagtcgg ccgtacgggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     4921 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     4981 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     5041 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     5101 cgtaaggaga aaataccgca tcaggcggcc ttaagggcct cgtgatacgc ctatttttat
     5161 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     5221 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5281 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     5341 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5401 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     5461 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5521 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5581 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5641 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5701 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5761 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5821 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5881 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5941 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     6001 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     6061 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     6121 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     6181 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     6241 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6301 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6361 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6421 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6481 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6541 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6601 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6661 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6721 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6781 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6841 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6901 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6961 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     7021 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     7081 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     7141 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     7201 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     7261 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     7321 aacaatt
//
