LOCUS       sequence-442622-        9325 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  sra-12 fluorescent neural reporter driving nuclear CyOFP1 expression
            (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9325)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 9325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY122
FEATURES             Location/Qualifiers
     source          1..9325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4684..4704
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4726..4746
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6916..6938)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7038..7057
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7135..7153)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7221..7325
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7326..7394,7395..8186)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7544..7563)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8357..8945
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8846..8865
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9099..9116
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9233..9254
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9269..9274,9275..9292,9293..9299)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9307..9323
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(9312..9325,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcaga ttgccaagat
      181 ttgggagaaa taaaaattta aattttgtta aaatgtttgt atgtttgttt tctgaaaaaa
      241 gttatatggt taacaataaa caagttttta taatttaaaa aaaaaagtcg atgtgaatta
      301 aaattgtaat ttcgaaataa attttgttgt agcgatattt tgagtctcca atttaaatta
      361 caaaagagct ggatgagagc aaatctatta aactcattga aacggaaaga ttccgtgcga
      421 aattgcatct cgcagaaaaa ttttgtaatt aattgaaata agcaaggatt gtttgattaa
      481 cgagtgtgct cactctagac agctagattg aattcagatt cacaagattt ttcagctgcc
      541 cttattgtcc acattcacac tttgtgagct ggcttggtta actaaaaatt ttttacaatt
      601 caagctattt caccaaaatg gatattcaaa tgccacagtc attaggaggg tttcaggtca
      661 aggtaatttt tcaatcaatt tccggattaa ttaaatttaa aatacataag atttgtggct
      721 agtcccttat cttattaaaa ataaacttcc gcatgagttc aattaatata cttttttgtt
      781 taatgatgac ttcaatgata aataattttt gcaaaaaatt ctagctcagc tatttttaat
      841 tcgactggag aaaaataatt tttttcaaaa tattcccctt atctgaaaat ctcgcttcag
      901 cactaatatt tattctttaa attaccattt cccaatcaaa gtgcatttat taaagttcat
      961 gtgaatttca taaagaaaat gctttctttt cggaaatttt tcactcaata attatagcac
     1021 ttttcgccaa cttttattct atagtcactg cacactaaaa ttatgtctga tatcatttac
     1081 atactgatgt ttggcattct ttataaaact tttttttcag ctgaaattaa aaaaaaattt
     1141 tttttagctg tgagtcgaaa ctgtattctg cgcaaaaagt ctgaaaaaaa tcccaataag
     1201 ttttcttttg ctgcgattac taaactgctt ttcacaagat ttcaaactct actctcttta
     1261 cattcctgta ttgtaacaat tgaaagctac ttgctctacg ctctttttaa tctttacccc
     1321 cgccgcacta accaattttt caaaaagtga attacataat caaacaggaa tagactaaga
     1381 ctaggaagta gcatgttcta ctcttggaaa tgtaatgttt cccgccgtaa ttgccttgtc
     1441 accatggacc aaaacgggcg gtaagcatgc atgtagagct tgtgattttg tgttttcagt
     1501 catttcctgc tgacacaaat ctacaaacaa tgtgacgttt acttgggctt tctggcacga
     1561 tttagtcatt caaattttaa aatatgagga gttatctttt gagttagaat tattatagac
     1621 gtagtagttt cttattcaaa aacttatttt cagaatacga taattttata tttttggtta
     1681 gatattttac ctctgcttga gtttgatttt ccagctctaa ctaaacaagt agctgagcaa
     1741 tcagcgaagt gcgtgggtgt ggccaacaat tatgattggt cggccggaaa ttgacttttc
     1801 tggaagaaat agttcaagtc tttctaattt tattgataac ccgctttagt caaaacaact
     1861 atagttctat ggataaactt ttcccccata tttttcctgt ttttttttgg ttccttgttc
     1921 gattttttgc tcattaacct atatcatgat aaccttacgg acattttcat gacgtgctgt
     1981 ctagggaatt ttgaatgtct tgaaagattt ttgaatttta atcaactgga aaagtatggt
     2041 atatgcaaaa atacatttta aatttagaga cactggaata ttcatgcggc aggtgaaaat
     2101 tacagaaatc attgttacac tgatttttga tcaatacaat aattatgaaa aattaattgt
     2161 cagtggggat tttgtataag aatactcatc cagaacattt aggttatcaa aaatttcagt
     2221 caaagtgaat ggctagtctt taaaaatttt ggtattcctg agtattttag tattgccata
     2281 taggttcaaa atttccattc acaattctta aaagaggatt tcctggcttg aattagtttg
     2341 cgtcaatttt aaaaaatttg tgctaccgta ttttcaagct ttcattatgt ttgtgaatat
     2401 tctacattat aaatatcaga aaattttaaa aaatttctta aaattgtaaa cgccatcgac
     2461 ttgtttaatg aaaaataaga ctctagacaa tttaaagttt tctcagaaac tttttcagga
     2521 acttgtccaa aactaatttt cttatcgtta acctattttt cctttttttt tgttcttgga
     2581 tatataaatt attgatgttt tcaatagaaa atatgaaaaa cttcccacaa tttcacgcaa
     2641 aaccttctga taattgcctt cgaaaaatga ttatctcctg caattttctg atttttcaaa
     2701 tgtttgtttt ttgaacattc tctagagtcg caaaacagca acgatctcaa actatttttt
     2761 aaatctcaga aattgcattc tgcttcacgt aacattcatg ttttgaactc attaattttg
     2821 ataaacctcc tccagcctca tgcacgtttt cgtgtttcat cagatgttgg aaacaaaatt
     2881 ttatttcgat ggaagatttt ttgatggttt ttgattttga aaatatttgc attagacttg
     2941 aggtctttta gtgactttta aaatatttca taaaatgtgt taaattcagt caaaagtggc
     3001 aattactcaa gttcaggttt ttctagaatt tggcaatttg ccaaatattt gccataaatc
     3061 tttgaaaaat ttataagatt ctagagtgtt ttgtttagaa ttttcagcct actctggggt
     3121 ccagagactg ggccatgaaa aaaacggctc ccagtatttt acaagattta cgttttttaa
     3181 tccctaaaat tttctagtaa atcttgaact atatttataa gtatagtata ggtataacga
     3241 aactttgccc atttgccgac caattttcaa actatcagct tgtcccataa gtttttgtac
     3301 tatttttttt ttgaaaaaaa tttatttctc tcaagcgaca agtagtacta ttcacacaag
     3361 tattcaccat tagtgtccac cacttgttgc catttactag gtaacatcat gatgccacgg
     3421 gagaagaaat ccggcgaacg cgatgagaag aaagtggaga gttcagtttt gagatgctct
     3481 tcgtcgtcga attgcttgtc gcgcatgtag tcactgagag acaagaacaa atggtagtcg
     3541 gttggtgcaa gatctggaga atatggtgga tgcggtaaaa cagtccaacc aagatcttgc
     3601 agcttttgga aagtcttctt ggcgacatga ggcctagcat tatcgtgaag aaaatatagt
     3661 ttttcatatt ttccgttggt cttttctgca actcggtcca attgggcaca atagtaatca
     3721 gcagtgatag ttttattagt tggcaacaat tcccagtgca cgggtccttg aacaccccac
     3781 cagacacaga tcataatctt ttttgggtga agatcaggct ttggcgtcgg tattcctttc
     3841 tcaccgatcg gaagccattg acgttttctg gaatggttaa catagagcac ccacttctca
     3901 tctccagtaa ccagattgtt cagccaatcg aactttcgac gaaaagttag aagttgagta
     3961 caaacgttga cccgagtgag cttctgtgat gccgaaagtt catgaggcac ccaagttcca
     4021 agttttgaag taaaaccaag gcgacccaag tgacgtgcaa ttgtgctatg acaacactca
     4081 agcttctctt ccatttcacg aagactaaga cgaggctctt cctccaccag cttcactagg
     4141 tcttcttcat ttaactctac cggtcgccca gaacgttcta tttcttcgag actgaagtcg
     4201 ccgttcttga acttttgaaa ccaactcttt gctgtattat aagagagtgc tccctcaccc
     4261 atcgcaccgc atatgtttcg ttccgcttcc attgctgaat gaccaagacg aaattcataa
     4321 agaagaagca atctaacgtc tcgacgttca agtttgatga ttgtcatcgt ggcaaagtag
     4381 aaatggatga aatgaatctt ttctgaaagg ggacgacccg accttgacac gacctacgat
     4441 gaaaaaattt taaaactttc tagaagtttt ggaaaaatat ttgaaaaaaa gtacaaaaac
     4501 ttatgggaca acctgataca tcctcaaact taaaacacca tttttccgta taaaaaatct
     4561 aatttcagac ccgggattgg ccaaaggacc caaaggtatg tttcgaatga tactaacata
     4621 acatagaaca ttttcaggag gacccttgct tggagggtac cgagctcaga aaaaatgacc
     4681 gctccaaaga agaagcgcaa ggttccagtc gagaagatga ctgccccaaa aaagaaacgt
     4741 aaggtcccgc gggtaagttt aaacagttcg gtactaacta accatacata tttaaatttt
     4801 cagcgcgctg tgtcaaaagg agaagagctt attaaggaga atatgcgttc caagctctac
     4861 ctcgagggat ccgtcaacgg acaccaattc aagtgcaccc acgagggaga gggaaagcca
     4921 tacgagggaa agcaaaccaa ccgtatcaag gtcgtcgagg gaggaccact cccattcgcc
     4981 ttcgacatcc tcgccaccca cttcatgtac ggatccaagg taagtttaaa catatatata
     5041 ctaactaacc ctgattattt aaattttcag gtcttcatca agtacccagc cgacctccca
     5101 gactacttca agcaatcctt cccagaggga ttcacctggg agcgtgtcat ggtcttcgag
     5161 gacggaggag tcctcaccgc cacccaagac acctccctcc aagacggaga gctcatctac
     5221 aacgtcaagg tccgtggagt caacttccca gccaacggac cagtcatgca aaagaagacc
     5281 ctcggatggg agccatccac cgagaccatg tacccagccg acggaggact cgagggacgt
     5341 tgcgacaagg taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag
     5401 gccctcaagc tcgtcggagg aggacacctc cacgtcaact tcaagaccac ctacaagtcc
     5461 aagaagccag tcaagatgcc aggagtccac tacgtcgacc gtcgtctcga gcgtatcaag
     5521 gaggccgaca acgagaccta cgtcgagcaa tacgagcacg ccgtcgcccg ttactccaac
     5581 ctcggaggag gaatggacga gctctacaag cctaggatac cggtgggtga agacccacca
     5641 aagccatctg ccaagggagc caagaaggcc gccaagaccg ttacgaagcc aaaggacgga
     5701 aagaagagac gtcatgcccg taaggaatca tactccgtct acatctaccg tgtcctcaag
     5761 caagttcatc cagacactgg agtttcctcc aaagccatgt ctatcatgaa ctcttttgtc
     5821 aacgatgtct tcgagcgtat tgctgctgaa gcatcccgtc ttgctcacta caacaagcgt
     5881 tccacaatct catcccgcga aattcagacc gctgtccgtc tgatccttcc aggagagctt
     5941 gccaagcacg ccgtgtctga gggaaccaag gccgttacca agtacacttc cagcaagtag
     6001 gaattccaac tgagcgccgg tcgctaccat taccaacttg tctggtgtca aaaataatag
     6061 gggccgctgt catcagagta agtttaaact gagttctact aactaacgag taatatttaa
     6121 attttcagca tctcgcgccc gtgcctctga cttctaagtc caattactct tcaacatccc
     6181 tacatgctct ttctccctgt gctcccaccc cctatttttg ttattatcaa aaaacttctc
     6241 ttaatttctt tgttttttag cttcttttaa gtcacctcta acaatgaaat tgtgtagatt
     6301 caaaaataga attaattcgt aataaaaagt cgaaaaaaat tgtgctccct ccccccatta
     6361 ataataattc tatcccaaaa tctacacaat gttctgtgta cacttcttat gttttttact
     6421 tctgataaat ttttttgaaa catcatagaa aaaaccgcac acaaaatacc ttatcatatg
     6481 ttacgtttca gtttatgacc gcaattttta tttcttcgca cgtctgggcc tctcatgacg
     6541 tcaaatcatg ctcatcgtga aaaagttttg gagtattttt ggaatttttc aatcaagtga
     6601 aagtttatga aattaatttt cctgcttttg ctttttgggg tttcccctat tgtttgtcaa
     6661 gatttcgagg acggcgtttt tcttgctaaa atcacaagta ttgatgagca cgatgcaaga
     6721 aagatcggaa gaaggtttgg gtttgaggct cagtggaagg tgagtagaag ttgataattt
     6781 gaaagtggag tagtgtctat ggggtttttg ccttaaatga cagaatacat tcccaatata
     6841 ccaaacataa ctgtttccta ctagtcggcc gtacgggccc tttcgtctcg cgcgtttcgg
     6901 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     6961 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     7021 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     7081 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcggcctt aagggcctcg
     7141 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     7201 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     7261 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     7321 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     7381 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     7441 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     7501 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     7561 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     7621 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     7681 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     7741 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     7801 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     7861 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     7921 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     7981 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     8041 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     8101 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     8161 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     8221 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     8281 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     8341 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     8401 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     8461 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     8521 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     8581 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     8641 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     8701 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     8761 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     8821 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     8881 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     8941 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     9001 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     9061 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     9121 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     9181 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     9241 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     9301 gtggaattgt gagcggataa caatt
//
