LOCUS       sequence-442627-        5324 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  srh-127 fluorescent neural reporter driving nuclear mNeptune2.5 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 5324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY125
FEATURES             Location/Qualifiers
     source          1..5324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             999..1019
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1044..1064
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1086..1106
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(2915..2937)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3037..3056
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3134..3152)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3220..3324
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3325..3393,3394..4185)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3543..3562)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4356..4944
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4845..4864
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5232..5253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5268..5273,5274..5291,5292..5298)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5306..5322
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(5311..5324,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt gcctatttgt
      181 cacattccgg aaatgcaaca aataaatgaa cacttgtttt ttcagtacaa ctatatttaa
      241 acaactctat ttaaacaaaa agaaatttga aaaactggag gttgaaattg tttttaacaa
      301 ttctggaaca ttttaaagtt tttggaaact ctagcatatc tctagcattt ttcggaaagt
      361 tctagaaagt gtcaaaagct ttgtaaagct attaaaaaaa actgggaaac ttttaaagcc
      421 tctgggaatt cctggaaatt tctagagact tctcaaaaat actgagaaat tttacaaaac
      481 ttctgtagaa ttctggaaac tttgtagaat gtctggcaat atctgataat ttttgaaaag
      541 ttctagaaac ttttggtagc tttgcaaaac agttaaaaac tttcggaaat ttttgggaac
      601 tctgtgactt tttgaatagt gctctttttc atgactcatt ttttggttta ttacgtgaca
      661 aactccgtca ataataaaaa aaatccctcc tagacacctt aattatacct gttcatcttc
      721 tcatagatca gcagaaaaaa ttattgcatt ggcgcagtcg caggtgaaaa tacatattga
      781 acaaaatcag aactactcac tcaaattgat tcagttgcac atttcattct tctcccctca
      841 cctaccaaat tttcaaataa cccgggattg gccaaaggac ccaaaggtat gtttcgaatg
      901 atactaacat aacatagaac attttcagga ggacccttgg agggtaccgg ttttcccagt
      961 cacgacgcgt ggtaccgagc tcagaaaaaa tgaccgctcc aaagaagaaa cgcaaagtac
     1021 cgagctcaga aaaaatgacg gcacctaaaa agaaacgcaa agtaccggtc gccgagaaga
     1081 cagccccaaa aaagaagcgt aaagttccac gcgtcatggg agtaagttta aacagttcgg
     1141 tactaactaa ccatacatat ttaaattttc agcgcgctgt ctccaaggga gaggagctca
     1201 tcaaggagaa catgcacacc aagctctaca tggagggaac cgtcaacaac caccacttca
     1261 agtgcaccca cgaaggggag ggcaagccct acgagggcac ccagaccaac aggattaagg
     1321 tggtggaggg aggccccctg ccgttcgcat tcgacatcct ggccacctgc tttatgtacg
     1381 ggagcaagac cttcatcaac cacacccaag gaatcccaga cttcttcaag caatccttcg
     1441 taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag cctgagggct
     1501 tcacatggga gagagtcacc acatacgaag acgggggcgt gcttaccgtt acccaggaca
     1561 ccagcctcca ggacggctgc ttgatctaca acgtcaagct cagaggggtg aacttcccat
     1621 ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccagtacc gagaccctgt
     1681 accccgctga cggcggcctg gaaggcagat gcgacatggc cctgaagctc gtgggcgggg
     1741 gccacctgca ctgcaacctg aagaccacat acagatccaa gaaacccgct aagaacctca
     1801 agatgccagg agtaagttta aacatatata tactaactaa ccctgattat ttaaattttc
     1861 aggtctactt tgtggaccgc agactggaaa gaatcaagga ggccgacaat gagacctacg
     1921 tcgagcagca cgaggtggct gtggccagat actgcgacct ccctagcaaa ctggggcaca
     1981 aacttaatgg catggacgag ctgtacaagt aagcgccggt cgctaccatt accaacttgt
     2041 ctggtgtcaa aaataatagg ggccgctgtc atcagagtaa gtttaaactg agttctacta
     2101 actaacgagt aatatttaaa ttttcagcat ctcgcgcccg tgcctctgac ttctaagtcc
     2161 aattactctt caacatccct acatgctctt tctccctgtg ctcccacccc ctatttttgt
     2221 tattatcaaa aaacttctct taatttcttt gttttttagc ttcttttaag tcacctctaa
     2281 caatgaaatt gtgtagattc aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt
     2341 gtgctccctc cccccattaa taataattct atcccaaaat ctacacaatg ttctgtgtac
     2401 acttcttatg ttttttactt ctgataaatt tttttgaaac atcatagaaa aaaccgcaca
     2461 caaaatacct tatcatatgt tacgtttcag tttatgaccg caatttttat ttcttcgcac
     2521 gtctgggcct ctcatgacgt caaatcatgc tcatcgtgaa aaagttttgg agtatttttg
     2581 gaatttttca atcaagtgaa agtttatgaa attaattttc ctgcttttgc tttttggggt
     2641 ttcccctatt gtttgtcaag atttcgagga cggcgttttt cttgctaaaa tcacaagtat
     2701 tgatgagcac gatgcaagaa agatcggaag aaggtttggg tttgaggctc agtggaaggt
     2761 gagtagaagt tgataatttg aaagtggagt agtgtctatg gggtttttgc cttaaatgac
     2821 agaatacatt cccaatatac caaacataac tgtttcctac tagtcggccg tacgggccct
     2881 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     2941 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     3001 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     3061 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     3121 ggcggcctta agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     3181 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     3241 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3301 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     3361 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     3421 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     3481 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     3541 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     3601 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     3661 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     3721 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     3781 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     3841 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     3901 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     3961 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     4021 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     4081 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     4141 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     4201 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     4261 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     4321 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     4381 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     4441 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     4501 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     4561 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     4621 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     4681 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     4741 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     4801 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     4861 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     4921 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     4981 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     5041 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     5101 gagtcagtga gcgatgaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     5161 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     5221 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     5281 cttccggctc gtatgttgtg tggaattgtg agcggataac aatt
//
