LOCUS       sequence-442629-        5891 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  srh-15 fluorescent neural reporter driving nuclear CyOFP1 expression
            (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY126
FEATURES             Location/Qualifiers
     source          1..5891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1250..1270
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1292..1312
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3482..3504)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3604..3623
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3701..3719)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3787..3891
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3892..3960,3961..4752)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4110..4129)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4923..5511
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5412..5431
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5665..5682
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5799..5820
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5835..5840,5841..5858,5859..5865)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5873..5889
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(5878..5891,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt tgagatgtca
      181 tgctttgaag aattcagtat caactgtaca atattcaagc caaaatactt aaaatgaact
      241 gcgtggattt tcgtaggtgt tacggtgaaa agcggacgta acttcataac cagtgaatat
      301 atcattaaat gaaaaaatga aaatgttttt gtggtgtttt cggtatttca ttaaccaaat
      361 aaattcactt tttgctttga tattttacat ttttgaaatt tcaacaagtt ggaatacaaa
      421 ctaccaaatt ttttccaaaa ggaaaaatat gaaatagacg gcttccttat ttatttgctt
      481 cgttgttggt taaatttgaa aaaaaaattt aaaaaatttc ttttgtacaa atttcgtcaa
      541 ttttttgtag gaaatttccg ctagaactga aattatttct gaaaaaataa tattttttaa
      601 tgagacagct caaaagattt tatttcagat aattatcttg aaaaatatat atacgtcttt
      661 ttgatcattg ttgagatttt agccagaatt accaatccac ctgttatgat cgttcatttt
      721 ttatttagta aacaagtcaa ctgtatagaa atcgcagtca ctcatgtcag tttttggttg
      781 ttttcaattg tcgaaaatta aaagctgaaa aaatcagaga gagtcgtctg aaaatgagct
      841 tgttccaata atttcgaaga gtataacatt gttaattgaa aacaattgtg tgtgattaga
      901 aatggtgttt tatttgaaat gtttttttga taaacacata attggtttac ataaaactac
      961 cttccccaaa aacgatgtga tcattggtac actgattgat tgatcacttt cttccaagaa
     1021 aatttaatta ccagaacatg aatgcaattt gattttgaag tggacgtcga tgaactccct
     1081 catataacat ctggtgctgt tgtcagatat ttgttttcac tcttgatttt atacacccgg
     1141 gattggccaa aggacccaaa ggtatgtttc gaatgatact aacataacat agaacatttt
     1201 caggaggacc cttgcttgga gggtaccgag ctcagaaaaa atgaccgctc caaagaagaa
     1261 gcgcaaggtt ccagtcgaga agatgactgc cccaaaaaag aaacgtaagg tcccgcgggt
     1321 aagtttaaac agttcggtac taactaacca tacatattta aattttcagc gcgctgtgtc
     1381 aaaaggagaa gagcttatta aggagaatat gcgttccaag ctctacctcg agggatccgt
     1441 caacggacac caattcaagt gcacccacga gggagaggga aagccatacg agggaaagca
     1501 aaccaaccgt atcaaggtcg tcgagggagg accactccca ttcgccttcg acatcctcgc
     1561 cacccacttc atgtacggat ccaaggtaag tttaaacata tatatactaa ctaaccctga
     1621 ttatttaaat tttcaggtct tcatcaagta cccagccgac ctcccagact acttcaagca
     1681 atccttccca gagggattca cctgggagcg tgtcatggtc ttcgaggacg gaggagtcct
     1741 caccgccacc caagacacct ccctccaaga cggagagctc atctacaacg tcaaggtccg
     1801 tggagtcaac ttcccagcca acggaccagt catgcaaaag aagaccctcg gatgggagcc
     1861 atccaccgag accatgtacc cagccgacgg aggactcgag ggacgttgcg acaaggtaag
     1921 tttaaacatg attttactaa ctaactaatc tgatttaaat tttcaggccc tcaagctcgt
     1981 cggaggagga cacctccacg tcaacttcaa gaccacctac aagtccaaga agccagtcaa
     2041 gatgccagga gtccactacg tcgaccgtcg tctcgagcgt atcaaggagg ccgacaacga
     2101 gacctacgtc gagcaatacg agcacgccgt cgcccgttac tccaacctcg gaggaggaat
     2161 ggacgagctc tacaagccta ggataccggt gggtgaagac ccaccaaagc catctgccaa
     2221 gggagccaag aaggccgcca agaccgttac gaagccaaag gacggaaaga agagacgtca
     2281 tgcccgtaag gaatcatact ccgtctacat ctaccgtgtc ctcaagcaag ttcatccaga
     2341 cactggagtt tcctccaaag ccatgtctat catgaactct tttgtcaacg atgtcttcga
     2401 gcgtattgct gctgaagcat cccgtcttgc tcactacaac aagcgttcca caatctcatc
     2461 ccgcgaaatt cagaccgctg tccgtctgat ccttccagga gagcttgcca agcacgccgt
     2521 gtctgaggga accaaggccg ttaccaagta cacttccagc aagtaggaat tccaactgag
     2581 cgccggtcgc taccattacc aacttgtctg gtgtcaaaaa taataggggc cgctgtcatc
     2641 agagtaagtt taaactgagt tctactaact aacgagtaat atttaaattt tcagcatctc
     2701 gcgcccgtgc ctctgacttc taagtccaat tactcttcaa catccctaca tgctctttct
     2761 ccctgtgctc ccacccccta tttttgttat tatcaaaaaa cttctcttaa tttctttgtt
     2821 ttttagcttc ttttaagtca cctctaacaa tgaaattgtg tagattcaaa aatagaatta
     2881 attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa taattctatc
     2941 ccaaaatcta cacaatgttc tgtgtacact tcttatgttt tttacttctg ataaattttt
     3001 ttgaaacatc atagaaaaaa ccgcacacaa aataccttat catatgttac gtttcagttt
     3061 atgaccgcaa tttttatttc ttcgcacgtc tgggcctctc atgacgtcaa atcatgctca
     3121 tcgtgaaaaa gttttggagt atttttggaa tttttcaatc aagtgaaagt ttatgaaatt
     3181 aattttcctg cttttgcttt ttggggtttc ccctattgtt tgtcaagatt tcgaggacgg
     3241 cgtttttctt gctaaaatca caagtattga tgagcacgat gcaagaaaga tcggaagaag
     3301 gtttgggttt gaggctcagt ggaaggtgag tagaagttga taatttgaaa gtggagtagt
     3361 gtctatgggg tttttgcctt aaatgacaga atacattccc aatataccaa acataactgt
     3421 ttcctactag tcggccgtac gggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     3481 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     3541 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     3601 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     3661 gatgcgtaag gagaaaatac cgcatcaggc ggccttaagg gcctcgtgat acgcctattt
     3721 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     3781 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     3841 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     3901 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     3961 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4021 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4081 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     4141 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     4201 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     4261 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     4321 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     4381 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     4441 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4501 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     4561 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     4621 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     4681 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     4741 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     4801 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     4861 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     4921 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     4981 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5041 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     5101 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     5161 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     5221 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     5281 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     5341 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     5401 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     5461 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     5521 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     5581 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     5641 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     5701 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     5761 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     5821 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     5881 ggataacaat t
//
