LOCUS       sequence-442632-        5391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  srj-24 fluorescent neural reporter driving nuclear mNeptune2.5 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY127
FEATURES             Location/Qualifiers
     source          1..5391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1066..1086
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1111..1131
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1153..1173
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(2982..3004)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3104..3123
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3201..3219)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3287..3391
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3392..3460,3461..4252)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3610..3629)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4423..5011
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4912..4931
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5299..5320
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5335..5340,5341..5358,5359..5365)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5373..5389
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(5378..5391,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagc gcaatgttcc
      181 agctatcaat tataaagcag ctaatattag agaaataaat aattaaagtg aataattgga
      241 gtagagttgt catttttcaa gatgctccta gttagatatc aggtacttat tgaaaatttt
      301 ctggttgact catattccgt catcctcaac tgggggcgct atagaagttg ggcgcactgt
      361 attgttgatt atcgccagta aaaaacaaat taaaatatca aaataaaaat tgccaaaaaa
      421 tattggacat tttttataat ccaaaaaatc gaaacatctt ttaaaaaaat gttcagaaac
      481 ataactgaaa cattcaaata tttacctaac aaaaaattta aggtattttt cgaaaaaaaa
      541 gcaaaaaatc aagaaaatcg aaaagatcat caagaatttt tgaatatttg atttttgttg
      601 gaatattaat attttcaaca taaaaggaac ataaaacagg aagacaattt ttttcgaact
      661 gaagactgcc aaaaaatctg taaaacttat aaaattcctg ccaaattttt aatgaattat
      721 ataatactgt atatatgtca cctatttttc ctattgattc tgctctttct aaaatctttc
      781 tgatgttcct ctcgcccacg gagattcccc caacaagtat tgaaacacga ggtgttctct
      841 aatttcctat atgtttgtgt atatcctgtc tgtaatcaca ttaaaatgtt agaaatgtat
      901 tctatttact tacttaacgc tgtaaaaccc gggattggcc aaaggaccca aaggtatgtt
      961 tcgaatgata ctaacataac atagaacatt ttcaggagga cccttggagg gtaccggttt
     1021 tcccagtcac gacgcgtggt accgagctca gaaaaaatga ccgctccaaa gaagaaacgc
     1081 aaagtaccga gctcagaaaa aatgacggca cctaaaaaga aacgcaaagt accggtcgcc
     1141 gagaagacag ccccaaaaaa gaagcgtaaa gttccacgcg tcatgggagt aagtttaaac
     1201 agttcggtac taactaacca tacatattta aattttcagc gcgctgtctc caagggagag
     1261 gagctcatca aggagaacat gcacaccaag ctctacatgg agggaaccgt caacaaccac
     1321 cacttcaagt gcacccacga aggggagggc aagccctacg agggcaccca gaccaacagg
     1381 attaaggtgg tggagggagg ccccctgccg ttcgcattcg acatcctggc cacctgcttt
     1441 atgtacggga gcaagacctt catcaaccac acccaaggaa tcccagactt cttcaagcaa
     1501 tccttcgtaa gtttaaacat gattttacta actaactaat ctgatttaaa ttttcagcct
     1561 gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct taccgttacc
     1621 caggacacca gcctccagga cggctgcttg atctacaacg tcaagctcag aggggtgaac
     1681 ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc cagtaccgag
     1741 accctgtacc ccgctgacgg cggcctggaa ggcagatgcg acatggccct gaagctcgtg
     1801 ggcgggggcc acctgcactg caacctgaag accacataca gatccaagaa acccgctaag
     1861 aacctcaaga tgccaggagt aagtttaaac atatatatac taactaaccc tgattattta
     1921 aattttcagg tctactttgt ggaccgcaga ctggaaagaa tcaaggaggc cgacaatgag
     1981 acctacgtcg agcagcacga ggtggctgtg gccagatact gcgacctccc tagcaaactg
     2041 gggcacaaac ttaatggcat ggacgagctg tacaagtaag cgccggtcgc taccattacc
     2101 aacttgtctg gtgtcaaaaa taataggggc cgctgtcatc agagtaagtt taaactgagt
     2161 tctactaact aacgagtaat atttaaattt tcagcatctc gcgcccgtgc ctctgacttc
     2221 taagtccaat tactcttcaa catccctaca tgctctttct ccctgtgctc ccacccccta
     2281 tttttgttat tatcaaaaaa cttctcttaa tttctttgtt ttttagcttc ttttaagtca
     2341 cctctaacaa tgaaattgtg tagattcaaa aatagaatta attcgtaata aaaagtcgaa
     2401 aaaaattgtg ctccctcccc ccattaataa taattctatc ccaaaatcta cacaatgttc
     2461 tgtgtacact tcttatgttt tttacttctg ataaattttt ttgaaacatc atagaaaaaa
     2521 ccgcacacaa aataccttat catatgttac gtttcagttt atgaccgcaa tttttatttc
     2581 ttcgcacgtc tgggcctctc atgacgtcaa atcatgctca tcgtgaaaaa gttttggagt
     2641 atttttggaa tttttcaatc aagtgaaagt ttatgaaatt aattttcctg cttttgcttt
     2701 ttggggtttc ccctattgtt tgtcaagatt tcgaggacgg cgtttttctt gctaaaatca
     2761 caagtattga tgagcacgat gcaagaaaga tcggaagaag gtttgggttt gaggctcagt
     2821 ggaaggtgag tagaagttga taatttgaaa gtggagtagt gtctatgggg tttttgcctt
     2881 aaatgacaga atacattccc aatataccaa acataactgt ttcctactag tcggccgtac
     2941 gggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     3001 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     3061 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     3121 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     3181 cgcatcaggc ggccttaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     3241 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     3301 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     3361 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     3421 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     3481 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     3541 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     3601 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     3661 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     3721 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     3781 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     3841 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     3901 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     3961 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     4021 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     4081 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     4141 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     4201 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     4261 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     4321 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     4381 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4441 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     4501 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     4561 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     4621 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     4681 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4741 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     4801 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     4861 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4921 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4981 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     5041 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     5101 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     5161 gcgcagcgag tcagtgagcg atgaagcgga agagcgccca atacgcaaac cgcctctccc
     5221 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     5281 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     5341 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat t
//
