LOCUS       sequence-442640-        6012 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  sro-1 fluorescent neural reporter driving nuclear mNeptune2.5 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 6012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY128
FEATURES             Location/Qualifiers
     source          1..6012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1687..1707
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1732..1752
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1774..1794
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3603..3625)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3725..3744
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3822..3840)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3908..4012
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4013..4081,4082..4873)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4231..4250)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5044..5632
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5533..5552
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5920..5941
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5956..5961,5962..5979,5980..5986)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5994..6010
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(5999..6012,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcaga aattgggtgg
      181 aagagatcac ttgtcttttc tcttcttttt ttctcacttc tatttttgtt atgattctat
      241 ttttttcctt tcttccttct ccttaaaatc atttttccag attatctaaa tttattcact
      301 ccacttgttg ttttcatgtt ccatccttct ttttcagatc tactcgagtt tctaatcatc
      361 ttcatcctct atactctcta gatttatccc tcccatcaag aaaaagaagc aaaaaagaga
      421 gaacggaaat ccatgaaaat agttcaaatc catcatcatc cttccatttt cggctagctc
      481 ttggttttac gtgtgtgtgt gtgtgtgagt cgactttcat tcctgcacac aaaataaatg
      541 agaaactgga gatgaggaat tgtaaaatgc gcgaacctaa attgatagat gaggcctaca
      601 gaatagatga gtgaaggtga agaatgaacc cgaaaattgg atttaaaaaa tgaacaatga
      661 gaaagaaaaa tattccaaga ttgattggca gaggcttttc ccactacatt ttgcagaatt
      721 gtatttcttt tcgcatgact agaatctagc tccagaagtt tgcaagttac acagcaatta
      781 agttttatgt gggaataaat tgcaggcgca gttagggtat taaaaccacg aacacagcag
      841 taagagatat aatagttctt tggagaagaa aaacgtttac cgtatttcct ctattagtct
      901 tgcatgcaag actaattttc aattggtccg taggggtgtt taaacaacaa aaaacctttt
      961 ccaatatttc atcaattatt tgaacgcttt tgatcaaaaa ctcgagttca atttacccag
     1021 aaatgggcca atttattaac gttgcagcct ctatgcaaga tattgctgga ctgggaaaaa
     1081 gtcgggtgca agactattag agactgctgc aagactaata gaggaaatac ggtaattatt
     1141 aaataattta atgttaatgt tctggataaa ttctgtgatc actgcatatc gtaggcacaa
     1201 aacatcttcc taaaaaagca aagtaaacct acctcaagtc tacaatcaag gtgttggcaa
     1261 aattatccaa gtcctgacgg aacttatttc ctactcgcca caagcttaca cttccaacaa
     1321 tattcaaaaa catcattaca ttttgacaga cacctcaaaa catctgaaat ttatagttca
     1381 cttcaatgta tagttgtctg agaacgcgga aacagcaaac acttctactc ttcttgagca
     1441 agttgtcaat gctgcgacca aacgtttctt tatgtttcca cctgtttcta ccaactttcc
     1501 atgacaacaa gaacttattt caatttttcc ccaagtttcg aaacaaaacc cgggattggc
     1561 caaaggaccc aaaggtatgt ttcgaatgat actaacataa catagaacat tttcaggagg
     1621 acccttggag ggtaccggtt ttcccagtca cgacgcgtgg taccgagctc agaaaaaatg
     1681 accgctccaa agaagaaacg caaagtaccg agctcagaaa aaatgacggc acctaaaaag
     1741 aaacgcaaag taccggtcgc cgagaagaca gccccaaaaa agaagcgtaa agttccacgc
     1801 gtcatgggag taagtttaaa cagttcggta ctaactaacc atacatattt aaattttcag
     1861 cgcgctgtct ccaagggaga ggagctcatc aaggagaaca tgcacaccaa gctctacatg
     1921 gagggaaccg tcaacaacca ccacttcaag tgcacccacg aaggggaggg caagccctac
     1981 gagggcaccc agaccaacag gattaaggtg gtggagggag gccccctgcc gttcgcattc
     2041 gacatcctgg ccacctgctt tatgtacggg agcaagacct tcatcaacca cacccaagga
     2101 atcccagact tcttcaagca atccttcgta agtttaaaca tgattttact aactaactaa
     2161 tctgatttaa attttcagcc tgagggcttc acatgggaga gagtcaccac atacgaagac
     2221 gggggcgtgc ttaccgttac ccaggacacc agcctccagg acggctgctt gatctacaac
     2281 gtcaagctca gaggggtgaa cttcccatcc aacggccctg tgatgcagaa gaaaacactc
     2341 ggctgggagg ccagtaccga gaccctgtac cccgctgacg gcggcctgga aggcagatgc
     2401 gacatggccc tgaagctcgt gggcgggggc cacctgcact gcaacctgaa gaccacatac
     2461 agatccaaga aacccgctaa gaacctcaag atgccaggag taagtttaaa catatatata
     2521 ctaactaacc ctgattattt aaattttcag gtctactttg tggaccgcag actggaaaga
     2581 atcaaggagg ccgacaatga gacctacgtc gagcagcacg aggtggctgt ggccagatac
     2641 tgcgacctcc ctagcaaact ggggcacaaa cttaatggca tggacgagct gtacaagtaa
     2701 gcgccggtcg ctaccattac caacttgtct ggtgtcaaaa ataatagggg ccgctgtcat
     2761 cagagtaagt ttaaactgag ttctactaac taacgagtaa tatttaaatt ttcagcatct
     2821 cgcgcccgtg cctctgactt ctaagtccaa ttactcttca acatccctac atgctctttc
     2881 tccctgtgct cccaccccct atttttgtta ttatcaaaaa acttctctta atttctttgt
     2941 tttttagctt cttttaagtc acctctaaca atgaaattgt gtagattcaa aaatagaatt
     3001 aattcgtaat aaaaagtcga aaaaaattgt gctccctccc cccattaata ataattctat
     3061 cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt ttttacttct gataaatttt
     3121 tttgaaacat catagaaaaa accgcacaca aaatacctta tcatatgtta cgtttcagtt
     3181 tatgaccgca atttttattt cttcgcacgt ctgggcctct catgacgtca aatcatgctc
     3241 atcgtgaaaa agttttggag tatttttgga atttttcaat caagtgaaag tttatgaaat
     3301 taattttcct gcttttgctt tttggggttt cccctattgt ttgtcaagat ttcgaggacg
     3361 gcgtttttct tgctaaaatc acaagtattg atgagcacga tgcaagaaag atcggaagaa
     3421 ggtttgggtt tgaggctcag tggaaggtga gtagaagttg ataatttgaa agtggagtag
     3481 tgtctatggg gtttttgcct taaatgacag aatacattcc caatatacca aacataactg
     3541 tttcctacta gtcggccgta cgggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     3601 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     3661 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     3721 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     3781 agatgcgtaa ggagaaaata ccgcatcagg cggccttaag ggcctcgtga tacgcctatt
     3841 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     3901 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     3961 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     4021 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4081 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     4141 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     4201 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     4261 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     4321 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     4381 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     4441 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     4501 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     4561 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     4621 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     4681 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     4741 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     4801 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     4861 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     4921 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     4981 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5041 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5101 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5161 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     5221 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5281 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5341 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5401 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     5461 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     5521 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     5581 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     5641 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     5701 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     5761 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gatgaagcgg aagagcgccc
     5821 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     5881 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     5941 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     6001 cggataacaa tt
//
