LOCUS       sequence-442641-        5598 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  srz-103 fluorescent neural reporter driving nuclear mNeptune2.5 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY132
FEATURES             Location/Qualifiers
     source          1..5598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1273..1293
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1318..1338
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1360..1380
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3189..3211)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3311..3330
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3408..3426)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3494..3598
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3599..3667,3668..4459)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3817..3836)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4630..5218
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5119..5138
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5506..5527
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5542..5547,5548..5565,5566..5572)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5580..5596
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(5585..5598,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagc tgtagacttg
      181 ctgttcggca tgtcggcgca gtggtgttct ttgtgagtga gtgatttctg tctgttattt
      241 ttctacaatc caaaaatgta taatgaaatt ttcgttaact tgtttttctg ccagttggag
      301 actaagtgga aaattaaatt attttaagtg aactatttga atagaattta atgttatgta
      361 tgtctccaaa acttctctga aatggcgaac atcgttgata gatttggctg atcatgggtt
      421 ctaaaatgtt aaaccaaaag tggtgaaaac tgagtttgcg ccacttttca agaaatatta
      481 agcactgtcg catgttcaaa ctcttatact gttaaaatac gaaaggttta gacacgatta
      541 aaggtggagt accgaaatct ggaaaatatt ttcaaacgac tccaaatttg cccctgatta
      601 cgaatatcta tgtgaaaaaa tcaaaaaaaa aattccctga ctttttatat tagagcttga
      661 aatcgcgatt tttgtttgcg tcaccatgaa atatttcaaa tgcgcgccga aataaattat
      721 ccttggagcg tgtttgcctc atttaatttt ctctatatat ttttactttt ttcagttttt
      781 cagctaatag taaatcaagt gttttattca gtgggatata gcaaggatta cgtggctaaa
      841 attatattta aagcattttt gctgaaattg aacaggtgga aaaatggaag ttttgcctga
      901 tttatttaaa attgaaatgt tgaaactagg gctcatgccg taaagtactc aagctttgta
      961 cgtaacttct tgtgcttgaa caaaaatcaa tataatattt atcggaactg gtacttcagt
     1021 taatgttcag ttcgcaaggg tgatactgat caatatttca cctgctgctt tcaggaaatc
     1081 ttcggtaatt tttgaagtca aacattagac acaggttctt tacaaacact taaacccggg
     1141 attggccaaa ggacccaaag gtatgtttcg aatgatacta acataacata gaacattttc
     1201 aggaggaccc ttggagggta ccggttttcc cagtcacgac gcgtggtacc gagctcagaa
     1261 aaaatgaccg ctccaaagaa gaaacgcaaa gtaccgagct cagaaaaaat gacggcacct
     1321 aaaaagaaac gcaaagtacc ggtcgccgag aagacagccc caaaaaagaa gcgtaaagtt
     1381 ccacgcgtca tgggagtaag tttaaacagt tcggtactaa ctaaccatac atatttaaat
     1441 tttcagcgcg ctgtctccaa gggagaggag ctcatcaagg agaacatgca caccaagctc
     1501 tacatggagg gaaccgtcaa caaccaccac ttcaagtgca cccacgaagg ggagggcaag
     1561 ccctacgagg gcacccagac caacaggatt aaggtggtgg agggaggccc cctgccgttc
     1621 gcattcgaca tcctggccac ctgctttatg tacgggagca agaccttcat caaccacacc
     1681 caaggaatcc cagacttctt caagcaatcc ttcgtaagtt taaacatgat tttactaact
     1741 aactaatctg atttaaattt tcagcctgag ggcttcacat gggagagagt caccacatac
     1801 gaagacgggg gcgtgcttac cgttacccag gacaccagcc tccaggacgg ctgcttgatc
     1861 tacaacgtca agctcagagg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa
     1921 acactcggct gggaggccag taccgagacc ctgtaccccg ctgacggcgg cctggaaggc
     1981 agatgcgaca tggccctgaa gctcgtgggc gggggccacc tgcactgcaa cctgaagacc
     2041 acatacagat ccaagaaacc cgctaagaac ctcaagatgc caggagtaag tttaaacata
     2101 tatatactaa ctaaccctga ttatttaaat tttcaggtct actttgtgga ccgcagactg
     2161 gaaagaatca aggaggccga caatgagacc tacgtcgagc agcacgaggt ggctgtggcc
     2221 agatactgcg acctccctag caaactgggg cacaaactta atggcatgga cgagctgtac
     2281 aagtaagcgc cggtcgctac cattaccaac ttgtctggtg tcaaaaataa taggggccgc
     2341 tgtcatcaga gtaagtttaa actgagttct actaactaac gagtaatatt taaattttca
     2401 gcatctcgcg cccgtgcctc tgacttctaa gtccaattac tcttcaacat ccctacatgc
     2461 tctttctccc tgtgctccca ccccctattt ttgttattat caaaaaactt ctcttaattt
     2521 ctttgttttt tagcttcttt taagtcacct ctaacaatga aattgtgtag attcaaaaat
     2581 agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc cctcccccca ttaataataa
     2641 ttctatccca aaatctacac aatgttctgt gtacacttct tatgtttttt acttctgata
     2701 aatttttttg aaacatcata gaaaaaaccg cacacaaaat accttatcat atgttacgtt
     2761 tcagtttatg accgcaattt ttatttcttc gcacgtctgg gcctctcatg acgtcaaatc
     2821 atgctcatcg tgaaaaagtt ttggagtatt tttggaattt ttcaatcaag tgaaagttta
     2881 tgaaattaat tttcctgctt ttgctttttg gggtttcccc tattgtttgt caagatttcg
     2941 aggacggcgt ttttcttgct aaaatcacaa gtattgatga gcacgatgca agaaagatcg
     3001 gaagaaggtt tgggtttgag gctcagtgga aggtgagtag aagttgataa tttgaaagtg
     3061 gagtagtgtc tatggggttt ttgccttaaa tgacagaata cattcccaat ataccaaaca
     3121 taactgtttc ctactagtcg gccgtacggg ccctttcgtc tcgcgcgttt cggtgatgac
     3181 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     3241 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     3301 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     3361 ccgcacagat gcgtaaggag aaaataccgc atcaggcggc cttaagggcc tcgtgatacg
     3421 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     3481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     3541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     3601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     3661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     3721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     3781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     3841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     3901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     3961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     4021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     4081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     4141 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     4201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     4261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     4321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     4381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     4441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     4501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     4561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     4621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     4681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4741 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     4801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     5041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     5101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     5161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     5221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     5281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     5341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgatg aagcggaaga
     5401 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     5461 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     5521 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     5581 tgtgagcgga taacaatt
//
