LOCUS       sequence-442642-        8550 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tab-1 fluorescent neural reporter driving nuclear mTagBFP2 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8550)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 8550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY135
FEATURES             Location/Qualifiers
     source          1..8550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3915..3935
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3957..3977
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6141..6163)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6263..6282
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6360..6378)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6446..6550
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6551..6619,6620..7411)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6769..6788)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7582..8170
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8071..8090
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8324..8341
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8458..8479
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8494..8499,8500..8517,8518..8524)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8532..8548
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(8537..8550,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcaga gtcatatcca
      181 tgtccatttt ctgcaaatcg atatttttgg attaaagaga agttgaaaaa gagcaagaag
      241 acattacaac cgaacgggcc attgattaga taaagtttat ttacacttca caagcatatc
      301 acatgctatt ttcaaaaaaa aaaacgttcg gaaaatataa aaaatgaaac gagaaagagt
      361 aagaatcaat gcagaatcga gagaaatcgg atgaatggga atggtcagag agaagagaaa
      421 tgtctagtta ttttatcgtg tcgatttacg agacagacta cgagtcattc tccgtctgtg
      481 tcttctcatt tctccatgca atacccaagt ctatcaccaa cgctaggcaa gcaagcgagc
      541 aggcaggaaa agggcatttg gatgtgggaa aatggtttga tagtcagaga gacagatgac
      601 attttgttac tttcattgag aacgaatgta aacaataacg gtgtgtgata caacttcctg
      661 atgtgataac actattgaat taacctgcaa attttgttta taattcattc agtatttctc
      721 gagtagtaaa attgatattg gttttgaaaa tgaaaagttt gaacctatga tagattttat
      781 aagaatatac aaatagtttg aacacatttt atcaaaacgg aatgcgaagt tatttcgagc
      841 attagtttat gctgattcaa gtcgaaaaat tagttgcaat ctgagttaaa aaatatggta
      901 attcgcattg cacaataatt taataaaatt tcagaattct gggaaaagtc tgcgataatg
      961 cgtttacgta ctaggcccag aatgattttt caaaaaatta tttatcaaca cgttgagttc
     1021 gaagtttacg gtcaaaatgg gttttgagct attactgaga acgtttcata aattatcgag
     1081 gtaatgccaa aatgttgcac agctgtaatg tgttctaccg taacaacttt gttacacata
     1141 aattccaaaa aatgtcggca atcattaagt aattgaggtg aaccagaaat ttaataatgt
     1201 tttattgatc tagaaagcac accccctttc acatcgcaac aacagttctc aataagtttt
     1261 cccatttgct catccaatta ggaagaccgt aatccaacag tatgttattc tattgttttt
     1321 gtaggtgtct gtgagtgtgt cggcacatgc gtgccattag tgcaatattt gctcaattca
     1381 ttagttgttg ctacacatcc caatcaaaaa gaagagctgt ctgtcaaaag attacggaaa
     1441 gcaagagaga tgtcgcacca gacgcactta ctttatctca acactaatcc ttcggataca
     1501 gtttttgaaa atgcgtttat cggtttgccg actcttctca cctgcacacg ggttcaagtg
     1561 ggaatcattt ggaattagtg ctaaaggtgg tgatcagaat acgaaagggt cattatggtt
     1621 cccgcatttt agaatgtaca tataatttga aatgtatacg attatcgtgt tagcaaataa
     1681 ctaggaaatt ttttctgact acatttaaaa attttatctt aaataatcca tccattagtg
     1741 atattttggg acaagtgacg cggtcatgca tcgagttcag gcaagtttag taatagtggg
     1801 agtgtgcata gtttttaaat atgaaccctt ctttttaaat aatgaaccca acaagcaggt
     1861 ataactaggg gctgataatg gctggcttcc tgctggtccg ttctttatca aatttgtaca
     1921 tggaaaatac ttgaagatgt ttattttttt gaaaagactg tcaagagtgt gagaatttgc
     1981 tttttgaaaa tttgaatctg aatttttcaa atgtttaata actattttct tctactttct
     2041 gaaaaatctc ctttgcttaa aatccttcat taattgcaga attctcttct cactaatttg
     2101 caaaacatct tctcatcaaa tcttgttttc cactaaaatc acacagattt tcgtaagaat
     2161 aatgagcgcg ttttcaatga tgagctatat accgacaaaa cagcatgcca attgtgatga
     2221 aatagaagct tcaatacaac gactacttct tctacggtag tgaaatccgc taactaattg
     2281 ggaataattg ctgtattgca tatgcataag tttttgtatt aacaccacga gtatgaggga
     2341 ggagtgatga tgaagtaatt cttctggaac tatgttacat tggagctcac cacaggacac
     2401 ctttttgcgg tgactcaaga aacaatagag caagtcatga ggtgtcaaat gttcaaagta
     2461 cccatagagc cagttataat taagtgtgac atgtaaacgg aaggaacttt tcttaatatt
     2521 ctttgtcttg tatttgatga cgtggcaaca atattttgca cagcaggtct tactcctctt
     2581 gttgctaata agtttgtgtg agtatgtcta ctcaacataa taagttaaaa gccttctagc
     2641 gcttctgccg tctataatgc aataatagtc ttcgttactc gatgctcaat ttcttcgttt
     2701 agtcaggtca gccagtcgtc tttccctatt ttctctccaa gctttaaaac gtttgaaggg
     2761 gtacagtgat atgcacaaaa aatgcacaag ttgttgggga agtggtgaac atctgtggaa
     2821 aggttcaaat tgctcagttg cacaatgact attactagac gcgtgtcgct ctcaacacgt
     2881 tcacacttgt tattgttatt gtactggctc aatcgcattg tattgtaaca taaacacgta
     2941 attagaggga gatgatgagc atgagaggtc gaattgtgcc aaaagtggaa aacggctgta
     3001 agtaggcttt tcatgtaggc acaaacactg cctgtggcct ttgtctacca caaaagtact
     3061 gaaaggtgcg tagtaggcat gcctgaataa gtacaaaatt ttaagaataa gtacaagact
     3121 tacatcgtta gtgaatttga agagtttgca aaatcaaaat tattttgtca atcaagttaa
     3181 tacttctaga gtatttggcc aagaatcttt tgtagaccat ttgaaaattt tcagaaattg
     3241 agttgattgc taagcttttc caatttcttg atgagatatt tgaggtttga aaacattatg
     3301 taaattacgt tttgttttta ttggaatttt aatttcacct atatgctaga tatactattc
     3361 cagatcatca aaactggcaa gaaaagaaga ctccagacca tatacaaaaa atgaacacgt
     3421 catcataata cacgactgat cgaaaatgac acaactgatt agtttcaata ttttattcta
     3481 gtactgaaca ttacgagaca atcaatttcc atattttgct catcgtcact taagtggttg
     3541 tttattcttt catcaaccgc ctacaatatt gcctacggca atactgtcat tcacaatcat
     3601 tatccctctc atcaactctt ttttcttctt ctccttgtcg tctttcacgg gaccttcatc
     3661 tctattcgcc gactccgccc ccttccgatg ccgtctgaag ctccacccat tgtgctccta
     3721 tgggagcacc atattaccgt gttagctcat ttacacgaga atcttacctc ccgtaaaata
     3781 atcttcaaac ttatttgaaa cccgggattg gccaaaggac ccaaaggtat gtttcgaatg
     3841 atactaacat aacatagaac attttcagga ggacccttgc ttggagggta ccgagctcag
     3901 aaaaaatgac cgctccaaag aagaagcgca aggttccagt cgagaagatg actgccccaa
     3961 aaaagaaacg taaggtcccg cggatgtcag agcttattaa ggagaatatg catatgaaac
     4021 tgtacatgga aggaaccgtg gacaaccatc actttaagtg cacttcggag ggggaaggca
     4081 aaccgtacga gggcacccag accatgcgaa tcaaggtggt cgagggaggc ccactgccct
     4141 tcgccttcga cattctggta agtttaaaca tatatatact aactaaccct gattatttaa
     4201 attttcaggc tactagcttc ctgtacggca gcaagacttt tattaatcac acccagggca
     4261 tcccggactt cttcaaacag tcgttccctg agggcttcac atgggagcgt gtgaccacat
     4321 acgaagacgg tggcgttctc accgctaccc aggacaccag cctccaggac ggttgcctca
     4381 tatataatgt ggtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc
     4441 agaagatccg gggagtaaac ttcacatcca atggtccggt tatgcagaag aaaacactcg
     4501 gctgggaggc cttcaccgag acgctgtatc cggctgacgg aggcctggaa ggtcgaaacg
     4561 acatggccct gaagctcgtc ggcggtagcc atctcatcgc aaacgctaag accacatata
     4621 gatccaagaa accggtaagt ttaaacatga ttttactaac taactaatct gatttaaatt
     4681 ttcaggctaa gaaccttaaa atgccgggag tctactatgt tgactacaga ctggaacgga
     4741 tcaaggaggc caacaacgag acctacgtcg agcagcacga ggttgcagtt gccagatact
     4801 gcgacctccc gagcaaactg ggtcacaagc ttaatcctag gataccggtg ggtgaagacc
     4861 caccaaagcc atctgccaag ggagccaaga aggccgccaa gaccgttacg aagccaaagg
     4921 acggaaagaa gagacgtcat gcccgtaagg aatcatactc cgtctacatc taccgtgtcc
     4981 tcaagcaagt tcatccagac actggagttt cctccaaagc catgtctatc atgaactctt
     5041 ttgtcaacga tgtcttcgag cgtattgctg ctgaagcatc ccgtcttgct cactacaaca
     5101 agcgttccac aatctcatcc cgcgaaattc agaccgctgt ccgtctgatc cttccaggag
     5161 agcttgccaa gcacgccgtg tctgagggaa ccaaggccgt taccaagtac acttccagca
     5221 agtaggaatt ccaactgagc gccggtcgct accattacca acttgtctgg tgtcaaaaat
     5281 aataggggcc gctgtcatca gagtaagttt aaactgagtt ctactaacta acgagtaata
     5341 tttaaatttt cagcatctcg cgcccgtgcc tctgacttct aagtccaatt actcttcaac
     5401 atccctacat gctctttctc cctgtgctcc caccccctat ttttgttatt atcaaaaaac
     5461 ttctcttaat ttctttgttt tttagcttct tttaagtcac ctctaacaat gaaattgtgt
     5521 agattcaaaa atagaattaa ttcgtaataa aaagtcgaaa aaaattgtgc tccctccccc
     5581 cattaataat aattctatcc caaaatctac acaatgttct gtgtacactt cttatgtttt
     5641 ttacttctga taaatttttt tgaaacatca tagaaaaaac cgcacacaaa ataccttatc
     5701 atatgttacg tttcagttta tgaccgcaat ttttatttct tcgcacgtct gggcctctca
     5761 tgacgtcaaa tcatgctcat cgtgaaaaag ttttggagta tttttggaat ttttcaatca
     5821 agtgaaagtt tatgaaatta attttcctgc ttttgctttt tggggtttcc cctattgttt
     5881 gtcaagattt cgaggacggc gtttttcttg ctaaaatcac aagtattgat gagcacgatg
     5941 caagaaagat cggaagaagg tttgggtttg aggctcagtg gaaggtgagt agaagttgat
     6001 aatttgaaag tggagtagtg tctatggggt ttttgcctta aatgacagaa tacattccca
     6061 atataccaaa cataactgtt tcctactagt cggccgtacg ggccctttcg tctcgcgcgt
     6121 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     6181 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     6241 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     6301 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg gccttaaggg
     6361 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     6421 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     6481 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     6541 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     6601 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     6661 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     6721 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     6781 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     6841 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     6901 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     6961 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     7021 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     7081 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     7141 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     7201 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     7261 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     7321 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     7381 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     7441 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     7501 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     7561 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     7621 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     7681 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     7741 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     7801 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     7861 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     7921 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     7981 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8041 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8101 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8161 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     8221 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     8281 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     8341 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     8401 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     8461 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     8521 gttgtgtgga attgtgagcg gataacaatt
//
