LOCUS       sequence-442649-        7041 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7041)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 7041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRC1 CTFdeltaTA-FLAG
FEATURES             Location/Qualifiers
     source          1..7041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1733..1756
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    2471..2592
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2482..2501)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2536..2555
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     polyA_signal    2696..3090
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(3104..3478)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     enhancer        4071..4374
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(4454..5042)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4534..4553)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5150..5198
                     /note="synthetic polyadenylation signal"
     misc_feature    5209..5300
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5249..5268
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        5337..5548
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5561..5829
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     sig_peptide     5879..5938
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             5939..5965
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ggatgaagcg ctgaaagatg cgcagaccaa cgagaacctt tacttccaag acatcagccg
       61 tagagagaac ggagaagtgc tgcctctgaa aatcgtgaca tacgccgccg tgtccctctc
      121 cctggctgcc ctgctggtgg ctttcgtgct gctgtctctg gtgcggatgc ttcggagcaa
      181 cctgcatagc atccacaagc acctggccgt ggccctgttc ctgagccagc tggtctttgt
      241 gattggcatc aaccagacag agaatccttt cctgtgtaca gtggtggcca tcctgctcca
      301 ctatatctac atgagcacat tcgcctggac tctggtggaa agcctgcacg tgtacagaat
      361 gctgacagag gtgcgcaaca ttgacaccgg ccctatgcgg ttctactacg tggtcggatg
      421 gggcatcccc gctatcgtga ccggcctggc cgtgggcttg gatccccagg gctacggcaa
      481 ccccgacttc tgctggctga gcttgcagga caccctgatc tggtctttcg ccggccccat
      541 cggcgccgta atcatcatca acaccgttac atctgtgctg tctgctaagg tgtcctgtca
      601 gagaaagcac cactactacg gaaaaaaggg catcgtgagc ctgctgcgaa ccgccttcct
      661 gctactgctc ctgatcagcg ccacatggct gctgggcctg ctggccgtga acagagatgc
      721 cctgagcttt cactacctgt ttgccatctt cagcggcctg cagggcccct tcgtgctgct
      781 gttccactgc gtgctgaacc aggaggtgag aaagcacctg aagggcgtgc tgggaggcag
      841 gaagctgcac ctggaagatt ccgccaccac aagagccacc ctgctgacca gaagcctgaa
      901 ctgcaacacc accttcggcg acggacctga catgctgaga acagatctgg gcgagtctac
      961 cgccagcctg gactccatcg tgcgggacga gggcatccag aaactgggag tttcctccgg
     1021 cctggtgcgg ggatcccacg gcgaacccga cgccagcctc atgcctcgga gctgcaagga
     1081 cccaccaggc cacgacagcg attccgacag cgagctgagc ctggacgagc agagcagcag
     1141 ctacgcctct agccacagct ctgatagcga ggacgacggc gtgggcgccg aggaaaagtg
     1201 ggaccccgcc agaggcgccg tgcacagcac ccctaagggc gatgccgttg ctaatcacgt
     1261 gccagctggc tggcctgatc aatctctggc tgagagcgac agcgaggacc ccagcggcaa
     1321 gcctagactg aaggtggaaa ccaaggtgtc tgtggaactg cacagagagg aacaagggag
     1381 ccatagaggc gagtaccctc ctgatcagga gagcggcggc gcggctagac tggcctccag
     1441 ccagcctcct gagcagcgga aaggcatcct gaagaacaag gtcacatatc ctcctccact
     1501 gaccctgacc gagcagaccc tgaagggaag actgagagaa aagctggctg attgcgagca
     1561 aagtcctacc agctctagaa ccagctccct gggaagcgga ggtcctgatt gcgccatcac
     1621 agtgaagagc cccggcagag aacctggccg ggaccaccta aatggcgtcg ccatgaacgt
     1681 gcggaccggc tctgcccagg ccgacggcag cgactctgaa aaacctggtt ctgactacaa
     1741 agacgatgac gacaagtgaa gatctgacaa aactcacaca tgcccaccgt gcccagcacc
     1801 tgaactcctg gggggaccgt cagtcttcct cttcccccca aaacccaagg acaccctcat
     1861 gatctcccgg acccctgagg tcacatgcgt ggtggtggac gtgagccacg aagaccctga
     1921 ggtcaagttc aactggtacg tggacggcgt ggaggtgcat aatgccaaga caaagccgcg
     1981 ggaggagcag tacaacagca cgtaccgtgt ggtcagcgtc ctcaccgtcc tgcaccagga
     2041 ctggctgaat ggcaaggagt acaagtgcaa ggtctccaac aaagccctcc cagcccccat
     2101 cgagaaaacc atctccaaag ccaaagggca gccccgagaa ccacaggtgt acaccctgcc
     2161 cccatcccgg gaggagatga ccaagaacca ggtcagcctg acctgcctgg tcaaaggctt
     2221 ctatcccagc gacatcgccg tggagtggga gagcaatggg cagccggaga acaactacaa
     2281 gaccacgcct cccgtgctgg actccgacgg ctccttcttc ctctacagca agctcaccgt
     2341 ggacaagagc aggtggcagc aggggaacgt cttctcatgc tccgtgatgc acgaggctct
     2401 gcacaaccac tacacgcaga agagcctctc cctgtctccg ggtaaatgag tgctagctgg
     2461 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     2521 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     2581 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     2641 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggaatt aattctaaaa
     2701 tacagcatag caaaacttta acctccaaat caagcctcta cttgaatcct tttctgaggg
     2761 atgaataagg cataggcatc aggggctgtt gccaatgtgc attagctgtt tgcagcctca
     2821 ccttctttca tggagtttaa gatatagtgt attttcccaa ggtttgaact agctcttcat
     2881 ttctttatgt tttaaatgca ctgacctccc acattccctt tttagtaaaa tattcagaaa
     2941 taatttaaat acatcattgc aatgaaaata aatgtttttt attaggcaga atccagatgc
     3001 tcaaggccct tcataatatc ccccagttta gtagttggac ttagggaaca aaggaacctt
     3061 taatagaaat tggacagcaa gaaagcgagc ttctagctta tcctcagtcc tgctcctctg
     3121 ccacaaagtg cacgcagttg ccggccgggt cgcgcagggc gaactcccgc ccccacggct
     3181 gctcgccgat ctcggtcatg gccggcccgg aggcgtcccg gaagttcgtg gacacgacct
     3241 ccgaccactc ggcgtacagc tcgtccaggc cgcgcaccca cacccaggcc agggtgttgt
     3301 ccggcaccac ctggtcctgg accgcgctga tgaacagggt cacgtcgtcc cggaccacac
     3361 cggcgaagtc gtcctccacg aagtcccggg agaacccgag ccggtcggtc cagaactcga
     3421 ccgctccggc gacgtcgcgc gcggtgagca ccggaacggc actggtcaac ttggccatga
     3481 tggctcctcc tgtcaggaga ggaaagagaa gaaggttagt acaattgcta tagtgagttg
     3541 tattatacta tgcagatata ctatgccaat gattaattgt caaactaggg ctgcagggtt
     3601 catagtgcca cttttcctgc actgccccat ctcctgccca ccctttccca ggcatagaca
     3661 gtcagtgact taccaaactc acaggaggga gaaggcagaa gcttgagaca gacccgcggg
     3721 accgccgaac tgcgagggga cgtggctagg gcggcttctt ttatggtgcg ccggccctcg
     3781 gaggcagggc gctcggggag gcctagcggc caatctgcgg tggcaggagg cggggccgaa
     3841 ggccgtgcct gaccaatccg gagcacatag gagtctcagc cccccgcccc aaagcaaggg
     3901 gaagtcacgc gcctgtagcg ccagcgtgtt gtgaaatggg ggcttggggg ggttggggcc
     3961 ctgactagtc aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt
     4021 gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcat catggtaata
     4081 gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg
     4141 gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata
     4201 tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt
     4261 caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg
     4321 cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcctgca
     4381 ggttaattaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4441 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4501 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4561 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4621 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4681 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4741 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4801 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4861 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     4921 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4981 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     5041 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     5101 aagggatttt ggtcatggct agttaattaa catttaaatc agcggccgca ataaaatatc
     5161 tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgtaactaa catacgctct
     5221 ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc
     5281 aggtgccaga acatttctct atcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc
     5341 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg
     5401 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     5461 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     5521 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc
     5581 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc
     5641 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc
     5701 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac
     5761 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg
     5821 ttacagatcc aagctgtgac cggcgcctac ctgagatcac cggcgaagga gggccaccat
     5881 gtacaggatg caactcctgt cttgcattgc actaagtctt gcacttgtca cgaattcgta
     5941 cccatacgat gttccagatt acgctggatc caaaattgaa gaaggcaaac tggtgatttg
     6001 gattaacggc gataaaggct ataacggcct ggcggaagtg ggcaaaaaat ttgaaaaaga
     6061 taccggcatt aaagtgaccg tggaacatcc ggataaactg gaagaaaaat ttccgcaggt
     6121 ggcggcgacc ggcgatggcc cggatattat tttttgggcg catgatcgct ttggcggcta
     6181 tgcgcagagc ggcctgctgg cggaaattac cccggataaa gcgtttcagg ataaactgta
     6241 tccgtttacc tgggatgcgg tgcgctataa cggcaaactg attgcgtatc cgattgcggt
     6301 ggaagcgctg agcctgattt ataacaaaga tctgctgccg aacccgccga aaacctggga
     6361 agaaattccg gcgctggata aagaactgaa agcgaaaggc aaaagcgcgc tgatgtttaa
     6421 cctgcaggaa ccgtatttta cctggccgct gattgcggcg gatggcggct atgcgtttaa
     6481 atatgaaaac ggcaaatatg atattaaaga tgtgggcgtg gataacgcgg gcgcgaaagc
     6541 gggcctgacc tttctggtgg atctgattaa aaacaaacat atgaacgcgg ataccgatta
     6601 tagcattgcg gaagcggcgt ttaacaaagg cgaaaccgcg atgaccatta acggcccgtg
     6661 ggcgtggagc aacattgata ccagcaaagt gaactatggc gtgaccgtgc tgccgacctt
     6721 taaaggccag ccgagcaaac cgtttgtggg cgtgctgagc gcgggcatta acgcggcgag
     6781 cccgaacaaa gaactggcga aagaatttct ggaaaactat ctgctgaccg atgaaggcct
     6841 ggaagcggtg aacaaagata aaccgctggg cgcggtggcg ctgaaaagct atgaagaaga
     6901 actggcgaaa gatccgcgca ttgcggcgac catggaaaac gcgcagaaag gcgaaattat
     6961 gccgaacatt ccgcagatga gcgcgttttg gtatgcggtg cgcaccgcgg tgattaacgc
     7021 ggcgagcggc cgccagaccg t
//
