LOCUS       sequence-442676-        5402 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3-exon minigene, with SRSF1 motif-rich middle exon, and hnRNPA1 
            motif-rich region in first intron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5402)
  TITLE     RNA molecules display distinctive organization at nuclear speckles
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: WT_H1-S1
FEATURES             Location/Qualifiers
     source          1..5402
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(19..37,38..60,61..79,80..102,103..121,122..144,
                     145..163,164..186,187..205,206..228,229..247,248..270,
                     271..289)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        322..360
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     357..381
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    2732..2866
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2756..2775)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2810..2829
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3299..3318)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(3412..3429)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3583..4171)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3663..3682)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4342..5133,5134..5202))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4965..4984
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5203..5307)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5375..5393
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttcactcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
       61 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
      121 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
      181 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
      241 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
      301 cgagctcggt acccgggtcg aggtaggcgt gtacggtggg aggcctatat aagcagagct
      361 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
      421 agacaccggg accgatccag cctccgcgta cggttcgacc gctgaactgc atcgtcgccg
      481 tgtcccagaa taagggcatc ggcaagaacg gagaccttcc ctggccaaag ctcaggtact
      541 ggctggattg ggttagggaa accgaggcgg ttcgctgaat cgggtcgagc acttggcgga
      601 gacgcgcggg ccaactactt agggacagtc atgaggggta ggcccgccgg ctgcagccct
      661 tgcccatgcc cgcggtgatc cccatgctgt gccagccttt gcccagaggc gctctagctg
      721 ggagcaaagt ccggtcactg ggcagcacca ccccccggac ttgcatgggt agccgctgag
      781 atggagcctg agcacacgcg gccgccaaac aattagggaa ccaaacaatt agggaaccaa
      841 acaattaggg aaccaaacaa ttagggaacc aaacaattag ggaaccaaac aattagggaa
      901 ccaaacaatt agggaaccaa acaattaggg aaccaaacaa ttagggaacc aaacaattag
      961 ggaaccaaac aattagggaa ccaaacaatt agggaaccaa acaattaggg aaccaaacaa
     1021 ttagggaacc aaacaattag ggaaccaaac aattagggaa ccaaacaatt agggaaccaa
     1081 acaattaggg aaccaaacaa ttagggaacc aaacaattag ggaaccaaac aattagggaa
     1141 ccaaacaatt agggaaccaa acaattaggg aaccaaacaa ttagggaacc aaacaatctc
     1201 tttttttcag gccaaacaag aggaacgcca aacaagagga acgccaaaca agaggaacgc
     1261 caaacaagag gaacgccaaa caagaggaac gccaaacaag aggaacgcca aacaagagga
     1321 acgccaaaca agaggaacgc caaacaagag gaacgccaaa caagaggaac gccaaacaag
     1381 aggaacgcca aacaagagga acgccaaaca agaggaacgc caaacaagag gaacgccaaa
     1441 caagaggaac gccaaacaac acaggtaagt ccaaacaagc tagctggcca gtgagatcca
     1501 agaatcttcc tgtctctgct gatccactga taggattaca agtacatgcc accaagccca
     1561 gcttcctctt accatctgct ggggaccaaa cttaggccct cattcctaca cagtgaatac
     1621 ttgactttgt tatcacccaa ccctaataaa taactcacta tccaaacaag ttgaaaccct
     1681 tagaattctg tgttgctcca gcatgatgtt gtggtaaacg ttaatacaat aagatgcaca
     1741 ggtcataagt gcacattagc taagtgttga caaagactta gacctacata acttaaccct
     1801 attagccctc cagaaagttc ctcattctcc attccaggca actttcatca caccacatca
     1861 tgtacaacta ctattgaagt tgttttccac tatagataca atgagatgtc acatacggct
     1921 ttgtgttttg atttgcaagt accaatcgag tatgaaatat ggagtggata ttggacattg
     1981 gccaccatct aaatactttg tgttaaaaga attggttttc ataatttgtt ttgtactgac
     2041 tgctggctag tcagattacc tgactagtat ggacaggatt ttgcaataat cataattctt
     2101 ttttcaggga accaccacaa ggagctcatt ttcttgccaa aagtctggac gaagccttaa
     2161 aacttattga acaaccagag ttagcagata aagtggagct gtcatggttt ggatagttgg
     2221 aggcagttcc gtttacaagg aagccatgaa tcagccaggc catctcagac tctttgtgac
     2281 aaggatcatg caggaatttg aaagtgacac gttcttccca gaaattgatt tggagaaata
     2341 taaacttctc ccagagtacc caggggtcct ttctgaagtc caggaggaaa aaggcatcaa
     2401 gtataaattt gaagtctatg agaagaaagg ctaacagaaa gatacttgct gattgacttc
     2461 aagttctact gctttcctcc taaaattatg catttttaca agaccatggg acttgtgttg
     2521 gctttagatc tatgagttat tctttcttta gagagggata gttaggaaga tgtatttgtt
     2581 ttgtggtacc agagatggaa cctgggatcc tgtgcatcct gggcaactgt tgtactctaa
     2641 gccactcccc aaagtcatgc cccagcccct gtataattct aaacaattag aattattttc
     2701 attttcatta gtctaaccag gttatctaga ggatccagac atgataagat acattgatga
     2761 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     2821 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     2881 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     2941 cctctacaaa tgtggtatgg ctgattatga tcctgcaagc ctcgtcgtcc tggccggacc
     3001 acgctatctg tgcaaggtcc ccggccccgg acgcgcgctc catgagcaga gcgcccgccg
     3061 ccgaggcgaa gactcgggcg gcgccctgcc cgtcccacca ggtcaacagg cggtaaccgg
     3121 cctcttcatc gggaatgcgc gccttcagca ttgccggcat gtccccctgg cggacgggaa
     3181 gtatccagct cgaccaagct tggcgagatt ttcaggagct aaggaagcta aaatggagaa
     3241 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
     3301 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgc attaatgaat
     3361 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     3421 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     3481 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     3541 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     3601 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     3661 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     3721 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     3781 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     3841 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     3901 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3961 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4021 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4081 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4141 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4201 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     4261 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     4321 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     4381 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     4441 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     4501 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     4561 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     4621 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     4681 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     4741 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     4801 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     4861 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     4921 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca
     4981 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     5041 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     5101 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     5161 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     5221 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     5281 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     5341 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     5401 tc
//
