LOCUS       sequence-442755-       11602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast recombinational cloning compatible Agrobacterium tumefaciens 
            ternary vector containing bar gene (for resistance against 
            glufosinate ammonium) on transfer DNA (TDNA)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11602)
  AUTHORS   Sidhu YS, Cairns TC, Chaudhari YK, Usher J, Talbot NJ, Studholme DJ,
            Csukai M, Haynes K
  TITLE     Exploitation of sulfonylurea resistance marker and non-homologous 
            end joining mutants for functional analysis in Zymoseptoria tritici.
  JOURNAL   Fungal Genet Biol. 2015 Jun;79:102-9. doi: 
            10.1016/j.fgb.2015.04.015.
  PUBMED    26092796
REFERENCE   2  (bases 1 to 11602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2015-06"; volume: "79:102-9. doi"; pages: " 
            10.1016/j.fgb.2015.04.015"
COMMENT     Sequence Label: pC-BAR-YR
FEATURES             Location/Qualifiers
     source          1..11602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             77..94
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      129..381
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    403..427
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1728..2357
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2786..3859
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3925..4119
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4355..4377)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4463..4603
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4518..4537
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4618..4635)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4789..5377)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4869..4888)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6802..7596)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             complement(7783..8586)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(8587..8802)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     misc_feature    10512..10536
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             11019..11570
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
ORIGIN
        1 ctctagagtc gacctgcagg catgcaagct ttaagaggag tccaccatgg tagatctgac
       61 tagtgttaac gctagccacc accaccacca ccacgtgtga attacaggtg accagctcga
      121 atttccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
      181 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
      241 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
      301 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
      361 tgtcatctat gttactagat cgggaattaa actatcagtg tttgacagga tatattggcg
      421 ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa
      481 aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca
      541 aagtactttg atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca
      601 gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct
      661 gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga
      721 ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc
      781 ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg
      841 ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc
      901 caggatgctt gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct
      961 ggcccgcagc acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg
     1021 cctgcgtagc ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt
     1081 gaccgtgttc gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag
     1141 cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc
     1201 ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc
     1261 ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga
     1321 agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc
     1381 cgacgccctg gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag
     1441 gacggccagg acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc
     1501 gggtacgtgt tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc
     1561 ggtttgtctg atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag
     1621 cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct
     1681 gcgtatatga tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg
     1741 ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg
     1801 ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc
     1861 gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga
     1921 cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc
     1981 cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg
     2041 tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc
     2101 gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag
     2161 gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg
     2221 agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc
     2281 ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta
     2341 aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct
     2401 aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga
     2461 cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa
     2521 gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca
     2581 gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag
     2641 gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag
     2701 gaacgggcgg ttggccaggc gtaagcggct gggttgcctg ccggccctgc aatggcactg
     2761 gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat
     2821 cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg
     2881 gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat
     2941 ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa
     3001 gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag
     3061 tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga
     3121 ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat
     3181 ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat
     3241 gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc
     3301 ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga
     3361 aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa
     3421 cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa
     3481 gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga
     3541 gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga
     3601 tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc
     3661 cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt
     3721 ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga
     3781 ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga
     3841 agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga
     3901 aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac attgggaacc caaagccgta
     3961 cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca
     4021 catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa
     4081 acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc
     4141 cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc
     4201 gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct
     4261 accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac
     4321 cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     4381 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     4441 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
     4501 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     4561 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc
     4621 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     4681 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     4741 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     4801 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     4861 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     4921 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     4981 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     5041 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     5101 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     5161 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     5221 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     5281 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     5341 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     5401 ggggtctgac gctcagtgga acgaaaactc acgttaaggg ctgatgaatc ccctaatgat
     5461 tttggtaaaa atcattaagt taaggtggat acacatcttg tcatatgatc aaatggtttc
     5521 gcgaaaaatc aataatcaga caacaagatg tgcgaactcg atattttaca cgactctctt
     5581 taccaattct gccccgaatt acacttaaaa cgactcaaca gcttaacgtt ggcttgccac
     5641 gccttacttg actgtaaaac tctcactctt accgaacttg gccgtaacct gccaaccaaa
     5701 gcgagaacaa aacataacat caaacgaatc gaccgattgt taggtaatcg tcacctccac
     5761 aaagagcgac tcgctgtata ccgttggcat gctagcttta tctgttcggg caatacgatg
     5821 cccattgtac ttgttgactg gtctgatatt cgtgagcaaa aacgacttat ggtattgcga
     5881 gcttcagtcg cactacacgg tcgttctgtt actctttatg agaaagcgtt cccgctttca
     5941 gagcaatgtt caaagaaagc tcatgaccaa tttctagccg accttgcgag cattctaccg
     6001 agtaacacca caccgctcat tgtcagtgat gctggcttta aagtgccatg gtataaatcc
     6061 gttgagaagc tgggttggta ctggttaagt cgagtaagag gaaaagtaca atatgcagac
     6121 ctaggagcgg aaaactggaa acctatcagc aacttacatg atatgtcatc tagtcactca
     6181 aagactttag gctataagag gctgactaaa agcaatccaa tctcatgcca aattctattg
     6241 tataaatctc gctctaaagg ccgaaaaaat cagcgctcga cacggactca ttgtcaccac
     6301 ccgtcaccta aaatctactc agcgtcggca aaggagccat gggttctagc aactaactta
     6361 cctgttgaaa ttcgaacacc caaacaactt gttaatatct attcgaagcg aatgcagatt
     6421 gaagaaacct tccgagactt gaaaagtcct gcctacggac taggcctacg ccatagccga
     6481 acgagcagct cagagcgttt tgatatcatg ctgctaatcg ccctgatgct tcaactaaca
     6541 tgttggcttg cgggcgttca tgctcagaaa caaggttggg acaagcactt ccaggctaac
     6601 acagtcagaa atcgaaacgt actctcaaca gttcgcttag gcatggaagt tttgcggcat
     6661 tctggctaca caataacaag ggaagactta ctcgtggctg caaccctact agctcaaaat
     6721 ttattcacac atggttacgc tttggggaaa ttatgagggg atctctcagc gttaagggat
     6781 tttggtcatg cattctaggt actaaaacaa ttcatccagt aaaatataat attttatttt
     6841 ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact gttcttcccc
     6901 gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc
     6961 gcttctccca agatcaataa agccacttac tttgccatct ttcacaaaga tgttgctgtc
     7021 tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc
     7081 atacagctcg cgcggatctt taaatggagt gtcttcttcc cagttttcgc aatccacatc
     7141 ggccagatcg ttattcagta agtaatccaa ttcggctaag cggctgtcta agctattcgt
     7201 atagggacaa tccgatatgt cgatggagtg aaagagcctg atgcactccg catacagctc
     7261 gataatcttt tcagggcttt gttcatcttc atactcttcc gagcaaagga cgccatcggc
     7321 ctcactcatg agcagattgc tccagccatc atgccgttca aagtgcagga cctttggaac
     7381 aggcagcttt ccttccagcc atagcatcat gtccttttcc cgttccacat cataggtggt
     7441 ccctttatac cggctgtccg tcatttttaa atataggttt tcattttctc ccaccagctt
     7501 atatacctta gcaggagaca ttccttccgt atcttttacg cagcggtatt tttcgatcag
     7561 ttttttcaat tccggtgata ttctcatttt agccatttat tatttccttc ctcttttcta
     7621 cagtatttaa agatacccca agaagctaat tataacaaga cgaactccaa ttcactgttc
     7681 cttgcattct aaaaccttaa ataccagaaa acagcttttt caaagttgtt ttcaaagttg
     7741 gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc ggttagtttt gctggccgca
     7801 tcttctcaaa tatgcttccc agcctgcttt tctgtaacgt tcaccctcta ccttagcatc
     7861 ccttcccttt gcaaatagtc ctcttccaac aataataatg tcagatcctg tagagaccac
     7921 atcatccacg gttctatact gttgacccaa tgcgtctccc ttgtcatcta aacccacacc
     7981 gggtgtcata atcaaccaat cgtaaccttc atctcttcca cccatgtctc tttgagcaat
     8041 aaagccgata acaaaatctt tgtcgctctt cgcaatgtca acagtaccct tagtatattc
     8101 tccagtagat agggagccct tgcatgacaa ttctgctaac atcaaaaggc ctctaggttc
     8161 ctttgttact tcttctgccg cctgcttcaa accgctaaca atacctgggc ccaccacacc
     8221 gtgtgcattc gtaatgtctg cccattctgc tattctgtat acacccgcag agtactgcaa
     8281 tttgactgta ttaccaatgt cagcaaattt tctgtcttcg aagagtaaaa aattgtactt
     8341 ggcggataat gcctttagcg gcttaactgt gccctccatg gaaaaatcag tcaagatatc
     8401 cacatgtgtt tttagtaaac aaattttggg acctaatgct tcaactaact ccagtaattc
     8461 cttggtggta cgaacatcca atgaagcaca caagtttgtt tgcttttcgt gcatgatatt
     8521 aaatagcttg gcagcaacag gactaggatg agtagcagca cgttccttat atgtagcttt
     8581 cgacatgatt tatcttcgtt tcctgcaggt ttttgttctg tgcagttggg ttaagaatac
     8641 tgggcaattt catgtttctt caacactaca tatgcgtata tataccaatc taagtctgtg
     8701 ctccttcctt cgttcttcct tctgttcgga gattaccgaa tcaaaaaaat ttcaaggaaa
     8761 ccgaaatcaa aaaaaagaat aaaaaaaaaa tgatgaattg aaaagctagc ttatcgatga
     8821 taagctgtca aacatgagaa ttaattccac ggactataga ctatacctag tatactccgt
     8881 ctactgtacg atacacttcc gctcaggtcc ttgtccttta acgaggcctt accactcttt
     8941 tgttactcta ttgatccagc tcagcaaagg cagtgtgatc taagattcta ttcgcgatgt
     9001 agtaaaacta gctagaccga gaaagagact agaaatgcaa aaggcacttc tacaatggct
     9061 gccatcatta ttatccgatg tgacgctgca gcttctcaat gatattcgaa tacgctttga
     9121 ggagatacag cctaatatcc gacaaactgt tttacagatt tacgatcgta cttgttaccc
     9181 atcattgaat tttgaacatc cgaacctggg agttttccct gaaacagata gtatatttga
     9241 acctgtataa taatatatag tctagcgctt tacggaagac aatgtatgta tttcggttcc
     9301 tggagaaact attgcatcta ttgcataggt aatcttgcac gtcgcatccc cggttcattt
     9361 tctgcgtttc catcttgcac ttcaatagca tatctttgtt aacgaagcat ctgtgcttca
     9421 ttttgtagaa caaaaatgca acgcgagagc gctaattttt caaacaaaga atctgagctg
     9481 catttttaca gaacagaaat gcaacgcgaa agcgctattt taccaacgaa gaatctgtgc
     9541 ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaagaatctg
     9601 agctgcattt ttacagaaca gaaatgcaac gcgagagcgc tattttacca acaaagaatc
     9661 tatacttctt ttttgttcta caaaaatgca tcccgagagc gctatttttc taacaaagca
     9721 tcttagatta ctttttttct cctttgtgcg ctctataatg cagtctcttg ataacttttt
     9781 gcactgtagg tccgttaagg ttagaagaag gctactttgg tgtctatttt ctcttccata
     9841 aaaaaagcct gactccactt cccgcgttta ctgattacta gcgaagctgc gggtgcattt
     9901 tttcaagata aaggcatccc cgattatatt ctataccgat gtggattgcg catactttgt
     9961 gaacagaaag tgatagcgtt gatgattctt cattggtcag aaaattatga acggtttctt
    10021 ctattttgtc tctatatact acgtatagga aatgtttaca ttttcgtatt gttttcgatt
    10081 cactctatga atagttctta ctacaatttt tttgtctaaa gagtaatact agagataaac
    10141 ataaaaaatg tagaggtcga gtttagatgc aagttcaagg agcgaaaggt ggatgggtag
    10201 gttatatagg gatatagcac agagatatat agcaaagaga tacttttgag caatgtttgt
    10261 ggaagcgccg cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac
    10321 cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc
    10381 atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg
    10441 actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt
    10501 tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat
    10561 aacacattgc ggacgttttt aatgtactga attaacgccg aattaattcc taggccacca
    10621 tgttgggccc ggcgcgccga attcgagctc ggtacccaac tgatattgaa ggagcatttt
    10681 ttgggcttgg ctggagctag tggaggtcaa caatgaatgc ctattttggt ttagtcgtcc
    10741 aggcggtgag cacaaaattt gtgtcgtttg acaagatggt tcatttaggc aactggtcag
    10801 atcagcccca cttgtagcag tagcggcggc gctcgaagtg tgactcttat tagcagacag
    10861 gaacgaggac attattatca tctgctgctt ggtgcacgat aacttggtgc gtttgtcaag
    10921 caaggtaagt ggacgacccg gtcatacctt cttaagttcg cccttcctcc ctttatttca
    10981 gattcaatct gacttaccta ttctacccaa gcatccaaat gagcccagaa cgacgcccgg
    11041 ccgacatccg ccgtgccacc gaggcggaca tgccggcggt ctgcaccatc gtcaaccact
    11101 acatcgagac aagcacggtc aacttccgta ccgagccgca ggaaccgcag gagtggacgg
    11161 acgacctcgt ccgtctgcgg gagcgctatc cctggctcgt cgccgaggtg gacggcgagg
    11221 tcgccggcat cgcctacgcg ggtccctgga aggcacgcaa cgcctacgac tggacggccg
    11281 aatcgaccgt gtacgtctcc ccccgccacc agcggacggg actgggctcc acgctctaca
    11341 cccacctgct gaagtccctg gaggcacagg gcttcaagag cgtggtcgct gtcatcgggc
    11401 tgcccaacga cccgagcgtg cgcatgcacg aggcgctcgg atatgccccc cgcggcatgc
    11461 tgcgggcggc cggcttcaag cacgggaact ggcatgacgt gggtttctgg cagctggact
    11521 tcagcctgcc ggttccgccc cgtccggtcc tgcccgtcac cgagatttag agtagatgcc
    11581 gaccgggaac cagttgggga tc
//
