LOCUS       sequence-442772-        1590 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vitro synthesis of mRNA for fluorescent protein mScarlet3 
            (control).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Karapidaki I, Handberg-Thorsager M, Momose T, Yasuo H, Genikhovich 
            G, Assaf S, Deleau C, Pang Y, Clayton P, Lohmann B, Rusciano ML, 
            Stranges M, Mathieu J, Zilliox M, Ustyantsev K, Salmon B, 
            Laplace-Builhe B, Koenig M, Colgren JJ, Arnone MI, Berezikov E, 
            Brunet T, Bucher G, Burkhardt P, Dickinson DJ, Houliston E, Huisken 
            J, Leclere L, Averof M
  TITLE     A toolkit for testing membrane-localising tags across species
  JOURNAL   bioRxiv 2024.11.12.623055
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.11.12.623055"
COMMENT     Sequence Label: 0_T7-mScarlet3
FEATURES             Location/Qualifiers
     source          1..1590
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     87..108
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             153..839
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
                     VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     primer_bind     551..570
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 taatacgact cactataggg cgaattgggt aaagtgcaag ttaaagtgaa tcaattaaaa
       61 gtaaccagca accaagtaaa tcaactgcaa ctactgaaat ctgccaagaa gtaattattg
      121 aatacaagaa gagaactctg ggcgccacaa ccatggatag caccgaggca gtgatcaagg
      181 agttcatgcg gttcaaggtg cacatggagg gctccatgaa cggccacgag ttcgagatcg
      241 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg agggtgacca
      301 agggtggccc cctgcccttc tcctgggaca tcctgtcccc tcagttcatg tacggctcca
      361 gggccttcac gaagcacccc gccgacatcc ccgactactg gaagcagtcc ttccccgagg
      421 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgctgtgtcc gtggcccagg
      481 acacctccct ggaggacggc accctgatct acaaggtgaa gctccgcggc accaacttcc
      541 ctcctgacgg ccccgtaatg cagaagaaga caatgggctg ggaagcatcc accgagcggt
      601 tgtaccccga ggacgtcgtg ctgaagggcg acattaagat ggccctgcgc ctgaaggacg
      661 gcggtcgcta cctggcggac ttcaagacca cctacagggc caagaagccc gtgcagatgc
      721 ccggcgcttt caacatcgac cgcaagttgg acatcacatc ccacaacgag gactacaccg
      781 tggtggaaca gtacgaacgc tccgtggccc gccactccac cggcggctcc ggtggctcct
      841 aagcgatcgc tatcgatgtt ataagttctg aaccgagaaa gaggggagaa tgtagagccg
      901 atagctgccc aatactgcca cgcccccagc agcccaggca tggaaaataa tacagagaaa
      961 atccacactt attaggtgct actgtaattt tttttaaatc gcttaaaatt attcggcgtt
     1021 tgcctagtgc aaggcccatt tatggacaat tgtttgccaa actcaaagtt tgccggtcgg
     1081 aggaaaagtt acaacgccaa aatgtgttga aaaatgcaaa agaaattatt aaaaacatta
     1141 aaaaattatt aaataatcta tgtaattctt gaatgtaaac tctgtgtaaa tactaaaaat
     1201 gaaaaacaaa atggaaagcg tagatggaga acagttgaat cgttatatat ttaaattaat
     1261 attgcattta tttccaaatt acagtgggag aagcgctgac cgacttcttg cagtgtactc
     1321 tcgaaataaa acccctgtct gtaccacgta aaacccctac taaaaattag tctcgcccgc
     1381 tgctcacagt tagtttagtt caattatacg ctactgttta atttattaac tgtacttatt
     1441 aatgcgcgtc atgaataatg attgactgtc ccttattttt tttttttgct aatttctgta
     1501 ttaaatttaa tgatttacca taaaaatata aataaaaatg acggtcgagt tgtaaaatta
     1561 ataaaaaaaa aaaacgaatg ctgcggccgc
//
