LOCUS       sequence-442777-        1677 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged 
            with the H-Ras membrane localisation signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Karapidaki I, Handberg-Thorsager M, Momose T, Yasuo H, Genikhovich 
            G, Assaf S, Deleau C, Pang Y, Clayton P, Lohmann B, Rusciano ML, 
            Stranges M, Mathieu J, Zilliox M, Ustyantsev K, Salmon B, 
            Laplace-Builhe B, Koenig M, Colgren JJ, Arnone MI, Berezikov E, 
            Brunet T, Bucher G, Burkhardt P, Dickinson DJ, Houliston E, Huisken 
            J, Leclere L, Averof M
  TITLE     A toolkit for testing membrane-localising tags across species
  JOURNAL   bioRxiv 2024.11.12.623055
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.11.12.623055"
COMMENT     Sequence Label: 1_T7-mScarlet3-Hras
FEATURES             Location/Qualifiers
     source          1..1677
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     87..108
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             153..839
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
                     VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     primer_bind     551..570
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 taatacgact cactataggg cgaattgggt aaagtgcaag ttaaagtgaa tcaattaaaa
       61 gtaaccagca accaagtaaa tcaactgcaa ctactgaaat ctgccaagaa gtaattattg
      121 aatacaagaa gagaactctg ggcgccacaa ccatggatag caccgaggca gtgatcaagg
      181 agttcatgcg gttcaaggtg cacatggagg gctccatgaa cggccacgag ttcgagatcg
      241 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg agggtgacca
      301 agggtggccc cctgcccttc tcctgggaca tcctgtcccc tcagttcatg tacggctcca
      361 gggccttcac gaagcacccc gccgacatcc ccgactactg gaagcagtcc ttccccgagg
      421 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgctgtgtcc gtggcccagg
      481 acacctccct ggaggacggc accctgatct acaaggtgaa gctccgcggc accaacttcc
      541 ctcctgacgg ccccgtaatg cagaagaaga caatgggctg ggaagcatcc accgagcggt
      601 tgtaccccga ggacgtcgtg ctgaagggcg acattaagat ggccctgcgc ctgaaggacg
      661 gcggtcgcta cctggcggac ttcaagacca cctacagggc caagaagccc gtgcagatgc
      721 ccggcgcttt caacatcgac cgcaagttgg acatcacatc ccacaacgag gactacaccg
      781 tggtggaaca gtacgaacgc tccgtggccc gccactccac cggcggctcc ggtggctccc
      841 gtgagatccg gcagcacaag ctgcggaagc tgaaccctcc tgatgagagt ggccccggct
      901 gcatgagctg caagtgtgtg ctctcctaag cgatcgctat cgatgttata agttctgaac
      961 cgagaaagag gggagaatgt agagccgata gctgcccaat actgccacgc ccccagcagc
     1021 ccaggcatgg aaaataatac agagaaaatc cacacttatt aggtgctact gtaatttttt
     1081 ttaaatcgct taaaattatt cggcgtttgc ctagtgcaag gcccatttat ggacaattgt
     1141 ttgccaaact caaagtttgc cggtcggagg aaaagttaca acgccaaaat gtgttgaaaa
     1201 atgcaaaaga aattattaaa aacattaaaa aattattaaa taatctatgt aattcttgaa
     1261 tgtaaactct gtgtaaatac taaaaatgaa aaacaaaatg gaaagcgtag atggagaaca
     1321 gttgaatcgt tatatattta aattaatatt gcatttattt ccaaattaca gtgggagaag
     1381 cgctgaccga cttcttgcag tgtactctcg aaataaaacc cctgtctgta ccacgtaaaa
     1441 cccctactaa aaattagtct cgcccgctgc tcacagttag tttagttcaa ttatacgcta
     1501 ctgtttaatt tattaactgt acttattaat gcgcgtcatg aataatgatt gactgtccct
     1561 tatttttttt ttttgctaat ttctgtatta aatttaatga tttaccataa aaatataaat
     1621 aaaaatgacg gtcgagttgt aaaattaata aaaaaaaaaa acgaatgctg cggccgc
//
