LOCUS       sequence-442785-        1644 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged 
            with the Lyn membrane localisation signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Karapidaki I, Handberg-Thorsager M, Momose T, Yasuo H, Genikhovich 
            G, Assaf S, Deleau C, Pang Y, Clayton P, Lohmann B, Rusciano ML, 
            Stranges M, Mathieu J, Zilliox M, Ustyantsev K, Salmon B, 
            Laplace-Builhe B, Koenig M, Colgren JJ, Arnone MI, Berezikov E, 
            Brunet T, Bucher G, Burkhardt P, Dickinson DJ, Houliston E, Huisken 
            J, Leclere L, Averof M
  TITLE     A toolkit for testing membrane-localising tags across species
  JOURNAL   bioRxiv 2024.11.12.623055
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.11.12.623055"
COMMENT     Sequence Label: 6_T7-Lyn-mScarlet3
FEATURES             Location/Qualifiers
     source          1..1644
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     87..108
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     regulatory      201..210
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             207..893
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
                     VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     primer_bind     605..624
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 taatacgact cactataggg cgaattgggt aaagtgcaag ttaaagtgaa tcaattaaaa
       61 gtaaccagca accaagtaaa tcaactgcaa ctactgaaat ctgccaagaa gtaattattg
      121 aatacaagaa gagaactctg ggcgccacaa ccatgggctg catcaagagc aagcgcaagg
      181 acaacctgaa cgacgacgag gccgccatgg atagcaccga ggcagtgatc aaggagttca
      241 tgcggttcaa ggtgcacatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
      301 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgagggtg accaagggtg
      361 gccccctgcc cttctcctgg gacatcctgt cccctcagtt catgtacggc tccagggcct
      421 tcacgaagca ccccgccgac atccccgact actggaagca gtccttcccc gagggcttca
      481 agtgggagcg cgtgatgaac ttcgaggacg gcggcgctgt gtccgtggcc caggacacct
      541 ccctggagga cggcaccctg atctacaagg tgaagctccg cggcaccaac ttccctcctg
      601 acggccccgt aatgcagaag aagacaatgg gctgggaagc atccaccgag cggttgtacc
      661 ccgaggacgt cgtgctgaag ggcgacatta agatggccct gcgcctgaag gacggcggtc
      721 gctacctggc ggacttcaag accacctaca gggccaagaa gcccgtgcag atgcccggcg
      781 ctttcaacat cgaccgcaag ttggacatca catcccacaa cgaggactac accgtggtgg
      841 aacagtacga acgctccgtg gcccgccact ccaccggcgg ctccggtggc tcctaagcga
      901 tcgctatcga tgttataagt tctgaaccga gaaagagggg agaatgtaga gccgatagct
      961 gcccaatact gccacgcccc cagcagccca ggcatggaaa ataatacaga gaaaatccac
     1021 acttattagg tgctactgta atttttttta aatcgcttaa aattattcgg cgtttgccta
     1081 gtgcaaggcc catttatgga caattgtttg ccaaactcaa agtttgccgg tcggaggaaa
     1141 agttacaacg ccaaaatgtg ttgaaaaatg caaaagaaat tattaaaaac attaaaaaat
     1201 tattaaataa tctatgtaat tcttgaatgt aaactctgtg taaatactaa aaatgaaaaa
     1261 caaaatggaa agcgtagatg gagaacagtt gaatcgttat atatttaaat taatattgca
     1321 tttatttcca aattacagtg ggagaagcgc tgaccgactt cttgcagtgt actctcgaaa
     1381 taaaacccct gtctgtacca cgtaaaaccc ctactaaaaa ttagtctcgc ccgctgctca
     1441 cagttagttt agttcaatta tacgctactg tttaatttat taactgtact tattaatgcg
     1501 cgtcatgaat aatgattgac tgtcccttat tttttttttt tgctaatttc tgtattaaat
     1561 ttaatgattt accataaaaa tataaataaa aatgacggtc gagttgtaaa attaataaaa
     1621 aaaaaaaacg aatgctgcgg ccgc
//
