LOCUS       sequence-442786-        1851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged 
            with the Src64B membrane localisation signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Karapidaki I, Handberg-Thorsager M, Momose T, Yasuo H, Genikhovich 
            G, Assaf S, Deleau C, Pang Y, Clayton P, Lohmann B, Rusciano ML, 
            Stranges M, Mathieu J, Zilliox M, Ustyantsev K, Salmon B, 
            Laplace-Builhe B, Koenig M, Colgren JJ, Arnone MI, Berezikov E, 
            Brunet T, Bucher G, Burkhardt P, Dickinson DJ, Houliston E, Huisken 
            J, Leclere L, Averof M
  TITLE     A toolkit for testing membrane-localising tags across species
  JOURNAL   bioRxiv 2024.11.12.623055
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.11.12.623055"
COMMENT     Sequence Label: 7_T7-Src64B-mScarlet3
FEATURES             Location/Qualifiers
     source          1..1851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     87..108
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             414..1100
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
                     VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     primer_bind     812..831
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 taatacgact cactataggg cgaattgggt aaagtgcaag ttaaagtgaa tcaattaaaa
       61 gtaaccagca accaagtaaa tcaactgcaa ctactgaaat ctgccaagaa gtaattattg
      121 aatacaagaa gagaactctg ggcgccacaa ccatgggcaa caaatgctgc agcaagcgac
      181 aggatcagga actggcactg gcctatccca ctgggggcta caagaaatcc gactacacct
      241 ttggccagac gcacatcaac agcagcggcg gcggcaacat gggcggcgtt cttggccaga
      301 agcataacaa cggtggctcg ctggactcgc gctacacgcc cgatcccaat catcggggtc
      361 cgttgaaaat cggcggaaag ggcggcgttg acatcatcag accacgcgga tccatggata
      421 gcaccgaggc agtgatcaag gagttcatgc ggttcaaggt gcacatggag ggctccatga
      481 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
      541 ccgccaagct gagggtgacc aagggtggcc ccctgccctt ctcctgggac atcctgtccc
      601 ctcagttcat gtacggctcc agggccttca cgaagcaccc cgccgacatc cccgactact
      661 ggaagcagtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
      721 gcgctgtgtc cgtggcccag gacacctccc tggaggacgg caccctgatc tacaaggtga
      781 agctccgcgg caccaacttc cctcctgacg gccccgtaat gcagaagaag acaatgggct
      841 gggaagcatc caccgagcgg ttgtaccccg aggacgtcgt gctgaagggc gacattaaga
      901 tggccctgcg cctgaaggac ggcggtcgct acctggcgga cttcaagacc acctacaggg
      961 ccaagaagcc cgtgcagatg cccggcgctt tcaacatcga ccgcaagttg gacatcacat
     1021 cccacaacga ggactacacc gtggtggaac agtacgaacg ctccgtggcc cgccactcca
     1081 ccggcggctc cggtggctcc taagcgatcg ctatcgatgt tataagttct gaaccgagaa
     1141 agaggggaga atgtagagcc gatagctgcc caatactgcc acgcccccag cagcccaggc
     1201 atggaaaata atacagagaa aatccacact tattaggtgc tactgtaatt ttttttaaat
     1261 cgcttaaaat tattcggcgt ttgcctagtg caaggcccat ttatggacaa ttgtttgcca
     1321 aactcaaagt ttgccggtcg gaggaaaagt tacaacgcca aaatgtgttg aaaaatgcaa
     1381 aagaaattat taaaaacatt aaaaaattat taaataatct atgtaattct tgaatgtaaa
     1441 ctctgtgtaa atactaaaaa tgaaaaacaa aatggaaagc gtagatggag aacagttgaa
     1501 tcgttatata tttaaattaa tattgcattt atttccaaat tacagtggga gaagcgctga
     1561 ccgacttctt gcagtgtact ctcgaaataa aacccctgtc tgtaccacgt aaaaccccta
     1621 ctaaaaatta gtctcgcccg ctgctcacag ttagtttagt tcaattatac gctactgttt
     1681 aatttattaa ctgtacttat taatgcgcgt catgaataat gattgactgt cccttatttt
     1741 ttttttttgc taatttctgt attaaattta atgatttacc ataaaaatat aaataaaaat
     1801 gacggtcgag ttgtaaaatt aataaaaaaa aaaaacgaat gctgcggccg c
//
