LOCUS       sequence-442787-        2118 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged 
            with the PH membrane localisation signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Karapidaki I, Handberg-Thorsager M, Momose T, Yasuo H, Genikhovich 
            G, Assaf S, Deleau C, Pang Y, Clayton P, Lohmann B, Rusciano ML, 
            Stranges M, Mathieu J, Zilliox M, Ustyantsev K, Salmon B, 
            Laplace-Builhe B, Koenig M, Colgren JJ, Arnone MI, Berezikov E, 
            Brunet T, Bucher G, Burkhardt P, Dickinson DJ, Houliston E, Huisken 
            J, Leclere L, Averof M
  TITLE     A toolkit for testing membrane-localising tags across species
  JOURNAL   bioRxiv 2024.11.12.623055
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.11.12.623055"
COMMENT     Sequence Label: 8_T7-PH-mScarlet3
FEATURES             Location/Qualifiers
     source          1..2118
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     87..108
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             681..1367
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
                     VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     primer_bind     1079..1098
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 taatacgact cactataggg cgaattgggt aaagtgcaag ttaaagtgaa tcaattaaaa
       61 gtaaccagca accaagtaaa tcaactgcaa ctactgaaat ctgccaagaa gtaattattg
      121 aatacaagaa gagaactctg ggcgccacaa ccatggactc gggccgggac ttcctgaccc
      181 tgcacggcct acaggatgat gaggatctac aggcgctgct gaagggcagc cagctcctga
      241 aggtgaagtc cagctcatgg aggagagagc gcttctacaa gttgcaggag gactgcaaga
      301 ccatctggca ggagtcccgc aaggtcatgc ggaccccgga gtcccagctg ttctccatcg
      361 aggacattca ggaggtgcga atggggcacc gcacggaggg tctggagaag ttcgcccgtg
      421 atgtgcccga ggaccgctgc ttctccattg tcttcaagga ccagcgcaat acactagacc
      481 tcatcgcccc atcgccagct gatgcccagc actgggtgct ggggctgcac aagatcatcc
      541 accactcagg ctccatggac cagcgtcaga agctacagca ctggattcac tcctgcttgc
      601 gaaaagctga caaaaacaag gacaacaaga tgagcttcaa ggagctgcag aacttcctga
      661 aggagctcaa catcctcgag atggatagca ccgaggcagt gatcaaggag ttcatgcggt
      721 tcaaggtgca catggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg
      781 agggccgccc ctacgagggc acccagaccg ccaagctgag ggtgaccaag ggtggccccc
      841 tgcccttctc ctgggacatc ctgtcccctc agttcatgta cggctccagg gccttcacga
      901 agcaccccgc cgacatcccc gactactgga agcagtcctt ccccgagggc ttcaagtggg
      961 agcgcgtgat gaacttcgag gacggcggcg ctgtgtccgt ggcccaggac acctccctgg
     1021 aggacggcac cctgatctac aaggtgaagc tccgcggcac caacttccct cctgacggcc
     1081 ccgtaatgca gaagaagaca atgggctggg aagcatccac cgagcggttg taccccgagg
     1141 acgtcgtgct gaagggcgac attaagatgg ccctgcgcct gaaggacggc ggtcgctacc
     1201 tggcggactt caagaccacc tacagggcca agaagcccgt gcagatgccc ggcgctttca
     1261 acatcgaccg caagttggac atcacatccc acaacgagga ctacaccgtg gtggaacagt
     1321 acgaacgctc cgtggcccgc cactccaccg gcggctccgg tggctcctaa gcgatcgcta
     1381 tcgatgttat aagttctgaa ccgagaaaga ggggagaatg tagagccgat agctgcccaa
     1441 tactgccacg cccccagcag cccaggcatg gaaaataata cagagaaaat ccacacttat
     1501 taggtgctac tgtaattttt tttaaatcgc ttaaaattat tcggcgtttg cctagtgcaa
     1561 ggcccattta tggacaattg tttgccaaac tcaaagtttg ccggtcggag gaaaagttac
     1621 aacgccaaaa tgtgttgaaa aatgcaaaag aaattattaa aaacattaaa aaattattaa
     1681 ataatctatg taattcttga atgtaaactc tgtgtaaata ctaaaaatga aaaacaaaat
     1741 ggaaagcgta gatggagaac agttgaatcg ttatatattt aaattaatat tgcatttatt
     1801 tccaaattac agtgggagaa gcgctgaccg acttcttgca gtgtactctc gaaataaaac
     1861 ccctgtctgt accacgtaaa acccctacta aaaattagtc tcgcccgctg ctcacagtta
     1921 gtttagttca attatacgct actgtttaat ttattaactg tacttattaa tgcgcgtcat
     1981 gaataatgat tgactgtccc ttattttttt tttttgctaa tttctgtatt aaatttaatg
     2041 atttaccata aaaatataaa taaaaatgac ggtcgagttg taaaattaat aaaaaaaaaa
     2101 aacgaatgct gcggccgc
//
