LOCUS       sequence-442788-        2079 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged 
            with the LactC2 membrane localisation signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Karapidaki I, Handberg-Thorsager M, Momose T, Yasuo H, Genikhovich 
            G, Assaf S, Deleau C, Pang Y, Clayton P, Lohmann B, Rusciano ML, 
            Stranges M, Mathieu J, Zilliox M, Ustyantsev K, Salmon B, 
            Laplace-Builhe B, Koenig M, Colgren JJ, Arnone MI, Berezikov E, 
            Brunet T, Bucher G, Burkhardt P, Dickinson DJ, Houliston E, Huisken 
            J, Leclere L, Averof M
  TITLE     A toolkit for testing membrane-localising tags across species
  JOURNAL   bioRxiv 2024.11.12.623055
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.11.12.623055"
COMMENT     Sequence Label: 9_T7-mScarlet3-LactC2
FEATURES             Location/Qualifiers
     source          1..2079
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     87..108
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             153..839
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
                     VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     primer_bind     551..570
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 taatacgact cactataggg cgaattgggt aaagtgcaag ttaaagtgaa tcaattaaaa
       61 gtaaccagca accaagtaaa tcaactgcaa ctactgaaat ctgccaagaa gtaattattg
      121 aatacaagaa gagaactctg ggcgccacaa ccatggatag caccgaggca gtgatcaagg
      181 agttcatgcg gttcaaggtg cacatggagg gctccatgaa cggccacgag ttcgagatcg
      241 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg agggtgacca
      301 agggtggccc cctgcccttc tcctgggaca tcctgtcccc tcagttcatg tacggctcca
      361 gggccttcac gaagcacccc gccgacatcc ccgactactg gaagcagtcc ttccccgagg
      421 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgctgtgtcc gtggcccagg
      481 acacctccct ggaggacggc accctgatct acaaggtgaa gctccgcggc accaacttcc
      541 ctcctgacgg ccccgtaatg cagaagaaga caatgggctg ggaagcatcc accgagcggt
      601 tgtaccccga ggacgtcgtg ctgaagggcg acattaagat ggccctgcgc ctgaaggacg
      661 gcggtcgcta cctggcggac ttcaagacca cctacagggc caagaagccc gtgcagatgc
      721 ccggcgcttt caacatcgac cgcaagttgg acatcacatc ccacaacgag gactacaccg
      781 tggtggaaca gtacgaacgc tccgtggccc gccactccac cggcggctcc ggtggctcct
      841 ccggactcag atcttgcact gaacccctag gcctgaagga taataccatc cccaacaagc
      901 agatcacagc ctccagctac tacaaaacct ggggcctgag tgcctttagc tggtttccct
      961 actacgcacg actggataat cagggcaagt tcaacgcctg gaccgcccag accaacagtg
     1021 cctctgagtg gctgcagatt gacctgggct cccagaagcg agtcacgggc atcatcaccc
     1081 agggtgcccg agactttggc cacattcaat atgtggctgc ctacagggtg gcctatggtg
     1141 atgatggtgt gacctggact gagtacaagg acccgggggc ctcagaaagc aagattttcc
     1201 ctggtaacat ggacaataat tcccacaaga agaacatatt tgagacgccg ttccaggctc
     1261 gcttcgtgcg gatccagccc gtggcctggc acaaccgtat caccctgcga gtggagctgc
     1321 tgggctgtta agcgatcgct atcgatgtta taagttctga accgagaaag aggggagaat
     1381 gtagagccga tagctgccca atactgccac gcccccagca gcccaggcat ggaaaataat
     1441 acagagaaaa tccacactta ttaggtgcta ctgtaatttt ttttaaatcg cttaaaatta
     1501 ttcggcgttt gcctagtgca aggcccattt atggacaatt gtttgccaaa ctcaaagttt
     1561 gccggtcgga ggaaaagtta caacgccaaa atgtgttgaa aaatgcaaaa gaaattatta
     1621 aaaacattaa aaaattatta aataatctat gtaattcttg aatgtaaact ctgtgtaaat
     1681 actaaaaatg aaaaacaaaa tggaaagcgt agatggagaa cagttgaatc gttatatatt
     1741 taaattaata ttgcatttat ttccaaatta cagtgggaga agcgctgacc gacttcttgc
     1801 agtgtactct cgaaataaaa cccctgtctg taccacgtaa aacccctact aaaaattagt
     1861 ctcgcccgct gctcacagtt agtttagttc aattatacgc tactgtttaa tttattaact
     1921 gtacttatta atgcgcgtca tgaataatga ttgactgtcc cttatttttt ttttttgcta
     1981 atttctgtat taaatttaat gatttaccat aaaaatataa ataaaaatga cggtcgagtt
     2041 gtaaaattaa taaaaaaaaa aaacgaatgc tgcggccgc
//
