LOCUS       sequence-442789-        1839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged 
            with a signal peptide and the CD8 transmembrane domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Karapidaki I, Handberg-Thorsager M, Momose T, Yasuo H, Genikhovich 
            G, Assaf S, Deleau C, Pang Y, Clayton P, Lohmann B, Rusciano ML, 
            Stranges M, Mathieu J, Zilliox M, Ustyantsev K, Salmon B, 
            Laplace-Builhe B, Koenig M, Colgren JJ, Arnone MI, Berezikov E, 
            Brunet T, Bucher G, Burkhardt P, Dickinson DJ, Houliston E, Huisken 
            J, Leclere L, Averof M
  TITLE     A toolkit for testing membrane-localising tags across species
  JOURNAL   bioRxiv 2024.11.12.623055
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.11.12.623055"
COMMENT     Sequence Label: 10_T7-SP-CD8tm-mScarlet3
FEATURES             Location/Qualifiers
     source          1..1839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     87..108
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             405..1088
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="DSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAKL
                     RVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGAV
                     SVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKMA
                     LRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHST
                     GGSGGS"
     primer_bind     800..819
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 taatacgact cactataggg cgaattgggt aaagtgcaag ttaaagtgaa tcaattaaaa
       61 gtaaccagca accaagtaaa tcaactgcaa ctactgaaat ctgccaagaa gtaattattg
      121 aatacaagaa gagaactctg ggcgccacaa ccatggatat cagctatatc ttcgtcatct
      181 gcctgatggc cctgtgcagc ggcggcagca gtctcagcca agtcgagggc aaacagaaat
      241 ccggaagggg ccggggctcc atgtggggca aggggaccgg attggacttc gcctgtgata
      301 tctacatctg ggcacccttg gccggcatct gcgtggccct tctgctgtcc ttgatcatca
      361 ctctcatctg ctaccacagc cgcacgggcc gcggtggtgg cggagatagc accgaggcag
      421 tgatcaagga gttcatgcgg ttcaaggtgc acatggaggg ctccatgaac ggccacgagt
      481 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
      541 gggtgaccaa gggtggcccc ctgcccttct cctgggacat cctgtcccct cagttcatgt
      601 acggctccag ggccttcacg aagcaccccg ccgacatccc cgactactgg aagcagtcct
      661 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gctgtgtccg
      721 tggcccagga cacctccctg gaggacggca ccctgatcta caaggtgaag ctccgcggca
      781 ccaacttccc tcctgacggc cccgtaatgc agaagaagac aatgggctgg gaagcatcca
      841 ccgagcggtt gtaccccgag gacgtcgtgc tgaagggcga cattaagatg gccctgcgcc
      901 tgaaggacgg cggtcgctac ctggcggact tcaagaccac ctacagggcc aagaagcccg
      961 tgcagatgcc cggcgctttc aacatcgacc gcaagttgga catcacatcc cacaacgagg
     1021 actacaccgt ggtggaacag tacgaacgct ccgtggcccg ccactccacc ggcggctccg
     1081 gtggctccta agcgatcgct atcgatgtta taagttctga accgagaaag aggggagaat
     1141 gtagagccga tagctgccca atactgccac gcccccagca gcccaggcat ggaaaataat
     1201 acagagaaaa tccacactta ttaggtgcta ctgtaatttt ttttaaatcg cttaaaatta
     1261 ttcggcgttt gcctagtgca aggcccattt atggacaatt gtttgccaaa ctcaaagttt
     1321 gccggtcgga ggaaaagtta caacgccaaa atgtgttgaa aaatgcaaaa gaaattatta
     1381 aaaacattaa aaaattatta aataatctat gtaattcttg aatgtaaact ctgtgtaaat
     1441 actaaaaatg aaaaacaaaa tggaaagcgt agatggagaa cagttgaatc gttatatatt
     1501 taaattaata ttgcatttat ttccaaatta cagtgggaga agcgctgacc gacttcttgc
     1561 agtgtactct cgaaataaaa cccctgtctg taccacgtaa aacccctact aaaaattagt
     1621 ctcgcccgct gctcacagtt agtttagttc aattatacgc tactgtttaa tttattaact
     1681 gtacttatta atgcgcgtca tgaataatga ttgactgtcc cttatttttt ttttttgcta
     1741 atttctgtat taaatttaat gatttaccat aaaaatataa ataaaaatga cggtcgagtt
     1801 gtaaaattaa taaaaaaaaa aaacgaatgc tgcggccgc
//
