LOCUS       sequence-442790-       13538 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast recombinational cloning compatible Agrobacterium tumefaciens 
            ternary vector containing sulfonylurea resistance gene (ILV alelle 
            from Magnaporthe oyrzae) on transfer DNA (TDNA)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13538)
  AUTHORS   Sidhu YS, Cairns TC, Chaudhari YK, Usher J, Talbot NJ, Studholme DJ,
            Csukai M, Haynes K
  TITLE     Exploitation of sulfonylurea resistance marker and non-homologous 
            end joining mutants for functional analysis in Zymoseptoria tritici.
  JOURNAL   Fungal Genet Biol. 2015 Jun;79:102-9. doi: 
            10.1016/j.fgb.2015.04.015.
  PUBMED    26092796
REFERENCE   2  (bases 1 to 13538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2015-06"; volume: "79:102-9. doi"; pages: " 
            10.1016/j.fgb.2015.04.015"
COMMENT     Sequence Label: pC-SUR-YR
FEATURES             Location/Qualifiers
     source          1..13538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    48..72
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(1094..1105)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             3151..3168
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3203..3455
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3477..3501
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4802..5431
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5860..6933
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6999..7193
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7429..7451)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7537..7677
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7592..7611
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7692..7709)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7863..8451)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7943..7962)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9876..10670)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             complement(10857..11660)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(11661..11876)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
ORIGIN
        1 ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat
       61 tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg
      121 tactgaatta acgccgaatt aattcctagg ccaccatgtt gggcccggcg cgccgaattc
      181 gagctcggta cccaactgat attgaaggag gtcgacgtgc caacgccaca gtgccccaca
      241 tctcccggct ggattcaccg ttgagcgggg attgtgtgga gggacttggt cgaaaatttg
      301 tgaacctccg atggcggcaa acatgactcc acgattcaca gcccaaaggc ctggccctgc
      361 ttcaattgtc atcgtctgac aggtccagga aaaatagtta gctggaggct cgttactttt
      421 ttttcccgct tcaacggcca agcaagcaag ctcatcgtct ctcgttcttt tctccattat
      481 tgacgactga gaacagattc gaaatgcttc gtactgttgg ccgcaaagcc ctgaggggct
      541 catccaaggg atgttcacga accatctcga ctctcaagcc cgccacggca actattgcca
      601 agcccggcag caggaccctt tcgacgccag cgacggcaac agcaacgtaa gtcagaatca
      661 cgagcgagcg aacaaacagg cagagttaca aacgccatca ctacgccttg tcttttcgtc
      721 tctgttacgt gtcgccaccc ccatgcgagc tacacgtctg cgcctgaagt cacaaggctt
      781 gcaatttcat gacgatgaac aacactgaca cttctgttac agagcacctc gaactaagcc
      841 cagcgccagc ttcaatgctc gccgcgatcc ccagcctctg gtcaaccctc gctcaggtga
      901 ggcagacgaa tcgtaagttg cgccaacacc gctcctacac cgccaagacc cataccgccg
      961 tcgcatttgg acaagcacaa gactgatttg accgtggcat agattcattg gcaagacggg
     1021 aggagagatt ttccacgaga tgatgctgag gcaaaacgtc aagcatatct gtaagcgtct
     1081 aattctacaa attcctaccc tcgatttcaa ccacatattc aaccacatat tctgacacat
     1141 gagtcacagt cggttaccct ggcggtgcta tccttcccgt gttcgacgcg atctacaact
     1201 cgaagcacat cgactttgtt ctgcccaagc atgagcaagg cgccggccac atggcagagg
     1261 gctatgctcg cgcttcaggc aaacccggcg ttgttctcgt cacctccggc cccggtgcca
     1321 caaatgtcat cactcccatg gccgacgctc ttgccgacgg tacacctctg gttgtattct
     1381 caggacaggt tgttacctct gatattggaa gcgacgcctt ccaggaggcc gacgtcatag
     1441 gcatctcccg gtcttgcacc aagtggaacg tcatggttaa gagcgctgac gagctcccga
     1501 ggagaattaa cgaggccttt gagattgcca ccagtgggcg acctgggcct gtcttggtcg
     1561 atctgcccaa ggacgtcacg gctagtgtgc tgaggagggc tatccccacc gagacctcga
     1621 ttccctctat tagcgcagca gcacgggctg tccaagaggc aggccgaaag cagcttgagc
     1681 actccatcaa acgcgtagcc gatctcgtca acattgccaa gaagcccgtc atatatgccg
     1741 gccaaggtgt cattttgtcg gaaggcggcg ttgaacttct caaggcgctt gccgacaagg
     1801 cctcgattcc tgtcaccacc actctgcatg gtctgggagc ctttgacgag ctcgacgaga
     1861 aggcactgca catgcttggt atgcacggtt cggcttatgc caacatgtcc atgcaagagg
     1921 ccgatttgat cattgccctt ggtggccgct tcgatgaccg tgtcactggc agcatcccca
     1981 aatttgctcc tgccgccaag ctagctgctg ctgaaggacg cggaggtatt gtccacttcg
     2041 agattatgcc caagaacatc aacaaggtcg tccaagcaac agaggccatt gagggcgacg
     2101 ttgcttcgaa cttgaagctg ttgctcccca agattgaaca acgatccatg accgatcgca
     2161 aggagtggtt cgaccagatc aaggagtgga aggagaagtg gcctctgtca cattatgaga
     2221 gggccgagcg tagtggtctc atcaagcctc agactctgat cgaggagctg agcaacctga
     2281 ctgctgaccg caaggacatg acctacatca caaccggtgt tggccagcac caaatgtgga
     2341 cagcacaaca tttcaggtgg aggcacccac ggtccatgat cacctctggc ggtttgggaa
     2401 ccatgggata tggtctgccg gcagcgattg gcgccaaggt tgctaggcca gatgctttgg
     2461 tcattgacat cgacggcgac gcatcgttca acatgactct gacagagctt tcgacggcgg
     2521 cacagttcaa cattggcgtc aaggtcattg tcttgaacaa cgaggagcag ggaatggtga
     2581 cccaatggca gaacttgttc tacgaggacc gctactcaca tacacaccag cgcaacccag
     2641 acttcatgaa gctcgccgat gcaatggacg ttcaacatcg ccgtgtttcg aagcctgacg
     2701 atgtcgttga tgctctgacg tggctgatca acaccgacgg ccccgctctg cttgaggtga
     2761 tgactgataa gaaggttcct gttctgccca tggtgcccgg aggtaacggc ctgcacgagt
     2821 tcatcacgtt tgatgccagt aagtaatata tccaatgcac tcctgtggcc cattttgagc
     2881 cttgggtatg attctagttc aacttgctaa ccagtgcatc aactcgtata ggcaaggata
     2941 agcaacggag agagctgatg cgcgcgagga cgaatggcct gcacggttaa gttgataatg
     3001 ggaattgatt attgcacggg aattgcatgc tctcacgtcg acagtagatg ccgaccggga
     3061 accagttggg gatcctctag agtcgacctg caggcatgca agctttaaga ggagtccacc
     3121 atggtagatc tgactagtgt taacgctagc caccaccacc accaccacgt gtgaattaca
     3181 ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt
     3241 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
     3301 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
     3361 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
     3421 attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttaaactatc agtgtttgac
     3481 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt
     3541 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt
     3601 tcccctcggg atcaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc
     3661 tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga
     3721 caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag
     3781 tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc
     3841 ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa
     3901 ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac
     3961 cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc
     4021 aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag
     4081 gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc
     4141 ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc
     4201 gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc
     4261 cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc
     4321 accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt
     4381 gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac
     4441 gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca
     4501 tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg
     4561 agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc
     4621 atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg
     4681 ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc
     4741 gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg
     4801 catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac
     4861 ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc
     4921 ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg
     4981 catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt
     5041 gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt
     5101 cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga
     5161 gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc
     5221 gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga
     5281 gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc
     5341 cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct
     5401 ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa
     5461 agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg
     5521 gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat
     5581 cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct
     5641 gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt
     5701 agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg
     5761 ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg cctgccggcc
     5821 ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc
     5881 ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg
     5941 caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg
     6001 gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt
     6061 aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg
     6121 ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac
     6181 cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca
     6241 gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat
     6301 ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc
     6361 cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa
     6421 gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg
     6481 aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc
     6541 tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat
     6601 tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat
     6661 tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca
     6721 ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga
     6781 gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag
     6841 tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac
     6901 ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt
     6961 gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg
     7021 aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt
     7081 gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc
     7141 taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct
     7201 ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta
     7261 cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta
     7321 cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc
     7381 ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     7441 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     7501 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
     7561 cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg
     7621 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     7681 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7741 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7801 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     7861 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     7921 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7981 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8041 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     8101 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     8161 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     8221 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8281 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     8341 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8401 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8461 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggctgatg
     8521 aatcccctaa tgattttggt aaaaatcatt aagttaaggt ggatacacat cttgtcatat
     8581 gatcaaatgg tttcgcgaaa aatcaataat cagacaacaa gatgtgcgaa ctcgatattt
     8641 tacacgactc tctttaccaa ttctgccccg aattacactt aaaacgactc aacagcttaa
     8701 cgttggcttg ccacgcctta cttgactgta aaactctcac tcttaccgaa cttggccgta
     8761 acctgccaac caaagcgaga acaaaacata acatcaaacg aatcgaccga ttgttaggta
     8821 atcgtcacct ccacaaagag cgactcgctg tataccgttg gcatgctagc tttatctgtt
     8881 cgggcaatac gatgcccatt gtacttgttg actggtctga tattcgtgag caaaaacgac
     8941 ttatggtatt gcgagcttca gtcgcactac acggtcgttc tgttactctt tatgagaaag
     9001 cgttcccgct ttcagagcaa tgttcaaaga aagctcatga ccaatttcta gccgaccttg
     9061 cgagcattct accgagtaac accacaccgc tcattgtcag tgatgctggc tttaaagtgc
     9121 catggtataa atccgttgag aagctgggtt ggtactggtt aagtcgagta agaggaaaag
     9181 tacaatatgc agacctagga gcggaaaact ggaaacctat cagcaactta catgatatgt
     9241 catctagtca ctcaaagact ttaggctata agaggctgac taaaagcaat ccaatctcat
     9301 gccaaattct attgtataaa tctcgctcta aaggccgaaa aaatcagcgc tcgacacgga
     9361 ctcattgtca ccacccgtca cctaaaatct actcagcgtc ggcaaaggag ccatgggttc
     9421 tagcaactaa cttacctgtt gaaattcgaa cacccaaaca acttgttaat atctattcga
     9481 agcgaatgca gattgaagaa accttccgag acttgaaaag tcctgcctac ggactaggcc
     9541 tacgccatag ccgaacgagc agctcagagc gttttgatat catgctgcta atcgccctga
     9601 tgcttcaact aacatgttgg cttgcgggcg ttcatgctca gaaacaaggt tgggacaagc
     9661 acttccaggc taacacagtc agaaatcgaa acgtactctc aacagttcgc ttaggcatgg
     9721 aagttttgcg gcattctggc tacacaataa caagggaaga cttactcgtg gctgcaaccc
     9781 tactagctca aaatttattc acacatggtt acgctttggg gaaattatga ggggatctct
     9841 cagcgttaag ggattttggt catgcattct aggtactaaa acaattcatc cagtaaaata
     9901 taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca
     9961 tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac
    10021 ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca
    10081 aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc
    10141 gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt
    10201 tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg
    10261 tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac
    10321 tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa
    10381 aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc
    10441 aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc
    10501 acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt
    10561 tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg
    10621 tatttttcga tcagtttttt caattccggt gatattctca ttttagccat ttattatttc
    10681 cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact
    10741 ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt
    10801 tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggttag
    10861 ttttgctggc cgcatcttct caaatatgct tcccagcctg cttttctgta acgttcaccc
    10921 tctaccttag catcccttcc ctttgcaaat agtcctcttc caacaataat aatgtcagat
    10981 cctgtagaga ccacatcatc cacggttcta tactgttgac ccaatgcgtc tcccttgtca
    11041 tctaaaccca caccgggtgt cataatcaac caatcgtaac cttcatctct tccacccatg
    11101 tctctttgag caataaagcc gataacaaaa tctttgtcgc tcttcgcaat gtcaacagta
    11161 cccttagtat attctccagt agatagggag cccttgcatg acaattctgc taacatcaaa
    11221 aggcctctag gttcctttgt tacttcttct gccgcctgct tcaaaccgct aacaatacct
    11281 gggcccacca caccgtgtgc attcgtaatg tctgcccatt ctgctattct gtatacaccc
    11341 gcagagtact gcaatttgac tgtattacca atgtcagcaa attttctgtc ttcgaagagt
    11401 aaaaaattgt acttggcgga taatgccttt agcggcttaa ctgtgccctc catggaaaaa
    11461 tcagtcaaga tatccacatg tgtttttagt aaacaaattt tgggacctaa tgcttcaact
    11521 aactccagta attccttggt ggtacgaaca tccaatgaag cacacaagtt tgtttgcttt
    11581 tcgtgcatga tattaaatag cttggcagca acaggactag gatgagtagc agcacgttcc
    11641 ttatatgtag ctttcgacat gatttatctt cgtttcctgc aggtttttgt tctgtgcagt
    11701 tgggttaaga atactgggca atttcatgtt tcttcaacac tacatatgcg tatatatacc
    11761 aatctaagtc tgtgctcctt ccttcgttct tccttctgtt cggagattac cgaatcaaaa
    11821 aaatttcaag gaaaccgaaa tcaaaaaaaa gaataaaaaa aaaatgatga attgaaaagc
    11881 tagcttatcg atgataagct gtcaaacatg agaattaatt ccacggacta tagactatac
    11941 ctagtatact ccgtctactg tacgatacac ttccgctcag gtccttgtcc tttaacgagg
    12001 ccttaccact cttttgttac tctattgatc cagctcagca aaggcagtgt gatctaagat
    12061 tctattcgcg atgtagtaaa actagctaga ccgagaaaga gactagaaat gcaaaaggca
    12121 cttctacaat ggctgccatc attattatcc gatgtgacgc tgcagcttct caatgatatt
    12181 cgaatacgct ttgaggagat acagcctaat atccgacaaa ctgttttaca gatttacgat
    12241 cgtacttgtt acccatcatt gaattttgaa catccgaacc tgggagtttt ccctgaaaca
    12301 gatagtatat ttgaacctgt ataataatat atagtctagc gctttacgga agacaatgta
    12361 tgtatttcgg ttcctggaga aactattgca tctattgcat aggtaatctt gcacgtcgca
    12421 tccccggttc attttctgcg tttccatctt gcacttcaat agcatatctt tgttaacgaa
    12481 gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca
    12541 aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct attttaccaa
    12601 cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg ctaatttttc
    12661 aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaga gcgctatttt
    12721 accaacaaag aatctatact tcttttttgt tctacaaaaa tgcatcccga gagcgctatt
    12781 tttctaacaa agcatcttag attacttttt ttctcctttg tgcgctctat aatgcagtct
    12841 cttgataact ttttgcactg taggtccgtt aaggttagaa gaaggctact ttggtgtcta
    12901 ttttctcttc cataaaaaaa gcctgactcc acttcccgcg tttactgatt actagcgaag
    12961 ctgcgggtgc attttttcaa gataaaggca tccccgatta tattctatac cgatgtggat
    13021 tgcgcatact ttgtgaacag aaagtgatag cgttgatgat tcttcattgg tcagaaaatt
    13081 atgaacggtt tcttctattt tgtctctata tactacgtat aggaaatgtt tacattttcg
    13141 tattgttttc gattcactct atgaatagtt cttactacaa tttttttgtc taaagagtaa
    13201 tactagagat aaacataaaa aatgtagagg tcgagtttag atgcaagttc aaggagcgaa
    13261 aggtggatgg gtaggttata tagggatata gcacagagat atatagcaaa gagatacttt
    13321 tgagcaatgt ttgtggaagc gccgcggtga tcacaggcag caacgctctg tcatcgttac
    13381 aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg
    13441 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc
    13501 tgacgccgtc ccggactgat gggctgcctg tatcgagt
//
