LOCUS       sequence-44280-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Larson DR, Zenklusen D, Wu B, Chao JA, Singer RH
  TITLE     Real-time observation of transcription initiation and elongation on 
            an endogenous yeast gene.
  JOURNAL   Science. 2011 Apr 22;332(6028):475-8.
  PUBMED    21512033
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-04-22"; volume: "332(6028)"; pages: "475-8"
COMMENT     Sequence Label: pDZ264 (pKAN tdtomato cyc loxP KAN loxP)
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(20..53)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(116..313)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 gtggatctga tatcacctaa taacttcgta tagcatacat tatacgaagt tatattaagg
       61 gttctcgaga gctcgttttc gacactggat ggcggcgtta gtatcgaatc gacagcagta
      121 tagcgaccag cattcacata cgattgacgc atgatattac tttctgcgca cttaacttcg
      181 catctgggca gatgatgtcg aggcgaaaaa aaatataaat cacgctaaca tttgattaaa
      241 atagaacaac tacaatataa aaaaactata caaatgacaa gttcttgaaa acaagaatct
      301 ttttattgtc agtactgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
      361 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
      421 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
      481 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
      541 atcaccatga gtgacgactg aatccggtga gaatggcaaa agcttatgca tttctttcca
      601 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
      661 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca
      721 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
      781 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttgccgg ggatcgcagt
      841 ggtgagtaac catgcatcat caggagtacg gat
//
