LOCUS       sequence-44281-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Wang JW, Beck ES, McCabe BD
  TITLE     A modular toolset for recombination transgenesis and neurogenetic 
            analysis of Drosophila.
  JOURNAL   PLoS One. 2012;7(7):e42102. Epub 2012 Jul 25.
  PUBMED    22848718
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(7)"; pages: "e42102. Epub 2012 Jul 25"
COMMENT     Sequence Label: pBID-UAS
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    147..180
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    193..623
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    669..763
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    787..881
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
ORIGIN
        1 catccagtgt ttgttccttg tgtagatgca tctcaaaaaa atggtgggca taatagtgtt
       61 gtttatatat atcaaaaata acaactataa taataagaat acatttaatt tagaaaatgc
      121 ttggatttca ctggaactag gctagcataa cttcgtataa tgtatgctat acgaagttat
      181 gctagcggat cctggccacg taataagtgt gcgttgaatt tattcgcaaa aacattgcat
      241 attttcggca aagtaaaatt ttgttgcata ccttatcaaa aaataagtgc tgcatacttt
      301 ttagagaaac caaataattt tttattgcat acccgttttt aataaaatac attgcatacc
      361 ctcttttaat aaaaaatatt gcatactttg acgaaacaaa ttttcgttgc atacccaata
      421 aaagattatt atattgcata cccgttttta ataaaataca ttgcataccc tcttttaata
      481 aaaaatattg catacgttga cgaaacaaat tttcgttgca tacccaataa aagattatta
      541 tattgcatac cttttcttgc cataccattt agccgatcaa ttgtgctcgg caacagtata
      601 tttgtggtgt gccaaccaac aacagatcca agctggccgc ggctcgatcc gcttgcatgc
      661 ctgcaggtcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct
      721 ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggaagc ttgcatgcct
      781 gcaggtcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc
      841 gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagactc tagcgagcgc
      901 cggagtataa atagaggc
//
