LOCUS       sequence-44282-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Wang JW, Beck ES, McCabe BD
  TITLE     A modular toolset for recombination transgenesis and neurogenetic 
            analysis of Drosophila.
  JOURNAL   PLoS One. 2012;7(7):e42102. Epub 2012 Jul 25.
  PUBMED    22848718
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(7)"; pages: "e42102. Epub 2012 Jul 25"
COMMENT     Sequence Label: pBID-UASC
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    138..171
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    184..614
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    660..754
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    778..872
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
ORIGIN
        1 tttgttcctt gtgtagatgc atctcaaaaa aatggtgggc ataatagtgt tgtttatata
       61 tatcaaaaat aacaactata ataataagaa tacatttaat ttagaaaatg cttggatttc
      121 actggaacta ggctagcata acttcgtata atgtatgcta tacgaagtta tgctagcgga
      181 tcctggccac gtaataagtg tgcgttgaat ttattcgcaa aaacattgca tattttcggc
      241 aaagtaaaat tttgttgcat accttatcaa aaaataagtg ctgcatactt tttagagaaa
      301 ccaaataatt ttttattgca tacccgtttt taataaaata cattgcatac cctcttttaa
      361 taaaaaatat tgcatacttt gacgaaacaa attttcgttg catacccaat aaaagattat
      421 tatattgcat acccgttttt aataaaatac attgcatacc ctcttttaat aaaaaatatt
      481 gcatacgttg acgaaacaaa ttttcgttgc atacccaata aaagattatt atattgcata
      541 ccttttcttg ccataccatt tagccgatca attgtgctcg gcaacagtat atttgtggtg
      601 tgccaaccaa caacagatcc aagctggccg cggctcgatc cgcttgcatg cctgcaggtc
      661 ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga
      721 gtactgtcct ccgagcggag tactgtcctc cgagcggaag cttgcatgcc tgcaggtcgg
      781 agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt
      841 actgtcctcc gagcggagta ctgtcctccg agcggagact ctagcgagct cgcccg
//
