LOCUS       sequence-442837-       10192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway backbone for C-terminal dTag cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10192)
  AUTHORS   Pulice JL, Meyerson M
  TITLE     Dosage amplification dictates oncogenic regulation by the NKX2-1 
            lineage factor in lung adenocarcinoma.
  JOURNAL   bioRxiv [Preprint]. 2023 Oct 27:2023.10.26.563996. doi: 
            10.1101/2023.10.26.563996.
  PUBMED    37994369
REFERENCE   2  (bases 1 to 10192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2023 Oct 27:2023.10.26.563996. doi: 
            10.1101/2023.10.26.563996."
COMMENT     Sequence Label: pLX311_CdTag-HA_EmptyVector
FEATURES             Location/Qualifiers
     source          1..10192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1766..1976
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1788..1984
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1835..1970
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2006..2401
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    2475..2592
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2730..3908
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2961..3899
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3856..3876
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3928..4052
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4077..4082,4083..4100,4101..4107)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4161..4820
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4227..4246)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5162..5467
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5346..5365
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5508..5632)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             5640..5960
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             5967..5993
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             5967..5993
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5997..6023
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    6045..6633
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6098..6118)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6516..6527)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6705..6938
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7016..7137
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7053..7072)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7107..7126
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7177..7312
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7332..7351)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7333..7351)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7361..7378)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7361..7377)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7370..7392)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7519..7974
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7606..7625)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7816..7837
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8000..8104
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8105..8173,8174..8965)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRILYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8323..8342)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9136..9724
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9625..9644
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9878..9895
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10012..10033
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10048..10053,10054..10071,10072..10078)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10086..10102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10091..10113
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10110..10126
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10110..10126
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10145..10165
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10147..10165
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggaccca tgcattgcat ctcaattagt cagcaaccag
     1741 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta
     1801 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     1861 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     1921 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     1981 caaaaagctt tctagaggta ccaccatggc caagcctttg tctcaagaag aatccaccct
     2041 cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc
     2101 cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac
     2161 tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa
     2221 cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg
     2281 gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga
     2341 tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg
     2401 cctgcagctg cagtagtaag gcgcgccgtt aacgaattct agatcttgag acaaatggca
     2461 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     2521 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     2581 attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg ccggctcgag
     2641 gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga
     2701 ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca
     2761 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     2821 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2881 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2941 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
     3001 cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg
     3061 atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc
     3121 cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct
     3181 ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt
     3241 gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc
     3301 tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc
     3361 gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt
     3421 ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct
     3481 gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg
     3541 gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt
     3601 cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg
     3661 agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt
     3721 taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg
     3781 aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg
     3841 gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg
     3901 tgtcgtgagg ctagcatcga ttgatcaaca agtttgtaca aaaaagctga acgagaaacg
     3961 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
     4021 tactgtaaaa cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta
     4081 cactttatgc ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt
     4141 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat
     4201 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
     4261 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
     4321 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc
     4381 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
     4441 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
     4501 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
     4561 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca
     4621 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg
     4681 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg
     4741 ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg
     4801 agtggcaggg cggggcgtaa agatctggat ccggcttact aaaagccaga taacagtatg
     4861 cgtatttgcg cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta
     4921 tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta
     4981 tcagttgctc aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag
     5041 aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct
     5101 gaggtcgccc ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga
     5161 aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt
     5221 acagagtgat attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg
     5281 tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag
     5341 ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt
     5401 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg
     5461 aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga
     5521 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat
     5581 tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg gttaccggtg
     5641 gagtgcaggt ggaaaccatc tccccaggag acgggcgcac cttccccaag cgcggccaga
     5701 cctgcgtggt gcactacacc gggatgcttg aagatggaaa gaaagttgat tcctcccggg
     5761 acagaaacaa gccctttaag tttatgctag gcaagcagga ggtgatccga ggctgggaag
     5821 aaggggttgc ccagatgagt gtgggtcaga gagccaaact gactatatct ccagattatg
     5881 cctatggtgc cactgggcac ccaggcatca tcccaccaca tgccactctc gtcttcgatg
     5941 tggagcttct aaaactggaa ggcggctacc cctacgacgt gcccgactac gccggctatc
     6001 cgtatgatgt cccggactat gcaggctaaa cgcgttaagt cgacaatcaa cctctggatt
     6061 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     6121 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     6181 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     6241 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     6301 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     6361 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     6421 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     6481 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     6541 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     6601 cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa
     6661 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     6721 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     6781 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     6841 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6901 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca
     6961 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     7021 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7081 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7141 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     7201 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7261 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
     7321 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     7381 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     7441 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     7501 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     7561 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     7621 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     7681 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     7741 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     7801 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     7861 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     7921 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     7981 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     8041 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     8101 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     8161 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     8221 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     8281 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     8341 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atactctatt ctcagaatga
     8401 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     8461 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     8521 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     8581 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     8641 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     8701 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     8761 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     8821 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     8881 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     8941 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     9001 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     9061 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     9121 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     9181 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     9241 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     9301 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     9361 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     9421 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     9481 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     9541 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     9601 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     9661 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     9721 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     9781 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     9841 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     9901 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     9961 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
    10021 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
    10081 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
    10141 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aa
//
