LOCUS       sequence-44285-e         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Wang JW, Beck ES, McCabe BD
  TITLE     A modular toolset for recombination transgenesis and neurogenetic 
            analysis of Drosophila.
  JOURNAL   PLoS One. 2012;7(7):e42102. Epub 2012 Jul 25.
  PUBMED    22848718
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(7)"; pages: "e42102. Epub 2012 Jul 25"
COMMENT     Sequence Label: pBID-UASC-FG
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             369..674
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     553..572
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ctgcgatgag tggcaggcgg ggcgtaatct agaggatccg gcttactaaa agccagataa
       61 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
      121 cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg
      181 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
      241 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
      301 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg
      361 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
      421 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
      481 gtgcacgtct gctgtcagat aaagtccccc gtgaacttta cccggtggtg catatcgggg
      541 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
      601 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
      661 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgacca
//
