LOCUS       sequence-442856-        5026 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 
            5-HT1AR coding frame.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Mallick A, Tan HL, Epstein JM, Gaudry Q, Dacks AM
  TITLE     Serotonin acts through multiple cellular targets during an olfactory
            critical period.
  JOURNAL   bioRxiv [Preprint]. 2024 Apr 14:2024.04.14.589413. doi: 
            10.1101/2024.04.14.589413.
  PUBMED    38645269
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Apr 14:2024.04.14.589413. doi: 
            10.1101/2024.04.14.589413."
COMMENT     Sequence Label: pTwist Amp_d5-HT1AR-7xsfGFP11-HA-HDR-donor
FEATURES             Location/Qualifiers
     source          1..5026
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1219..1266
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             1300..1347
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             1363..1410
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             1426..1473
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             1489..1536
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             1552..1599
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             1615..1662
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             1666..1692
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(2865..2881)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2865..2881)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2878..2900)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(3053..3641)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3133..3152)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(3758..3782)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(3882..4673,4674..4742))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSATITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4505..4524
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4743..4847)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4946..4968
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4960..4977
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4961..4977
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aattatgcta gctagaggac caggacgagc aggttggccc gcagcccaca accgcaacat
       61 cagcaatgac agcggcaggc accaacgagt cggaggacca atgcaaggcc aacggggtgg
      121 aggtcctgga ggatccgcag ttgcaacagc agctcgaaca agtgcagcag ctgcagaaat
      181 cggttaaatc cggcggtgga ggtggggcca gtacgtcaaa tgccaccaca attacgtcga
      241 tatcagcgct atcgccacaa acgccaacat cgcagggagt tggaatcgcc gccgccgccg
      301 ctggaccgat gacggccaaa accagcacgc tgacgagctg caaccagtcg catccgctat
      361 gcggcaccgc caacgaatcc ccatcgacac cagagccacg tagccgacaa ccgacgaccc
      421 cgcagcagca gccgcatcag caggcgcacc agcagcagca gcagcagcag caactctcca
      481 gcatcgccaa tccgatgcag aaggtgaaca agcgaaagga gaccctggag gccaaaaggg
      541 aacggaaggc ggccaagacg ctggccatca ttaccggcgc cttcgtcgtc tgctggctgc
      601 ccttcttcgt catggcactg accatgccgc tctgtgccgc ctgccagatc agcgattccg
      661 ttgcctcgct cttcctttgg ctgggctact tcaactccac cctcaatccg gtaaatggaa
      721 taataaataa atggctaagt aactgcagga atagtgcaaa actttgcata tatagagaaa
      781 ttttaagact tttaaacttc gcaacttgtc tgtgttcttt gaaaagagtt tttaaactat
      841 tagttacttt gaattttaat aatgaacttt taaaaatttg agagcatagt ttataataat
      901 atactcaaac tacagataga cttcttaatc ttaatctatt tcaaataatc agacaattta
      961 tgaaaaaaat tagagtttct taactcgttt tagttactta aattgttgtc aaatcaaatg
     1021 gttatgagtg cagttttcat tcccgtgcat tgttgattaa gtaattcccc cttgcaggtt
     1081 atttacacca tcttcagtcc ggagttccga caggccttca agcgcatcct cttcggcggc
     1141 caccgaccag tccactaccg cagcgggaag ctcggcgatg gaggaagcgg aggcgacgga
     1201 ggcagcggag gaggcagccg ggaccacatg gtgctgcacg agtacgtgaa cgccgccggc
     1261 atcactagcg gaggcgacgg aggcagcgga ggaggcagcc gggaccacat ggtgctgcac
     1321 gagtacgtga acgccgccgg catcactagc ggtagtggcg gccgtgacca catggtcctt
     1381 catgagtatg taaatgctgc tgggattaca ggtggctctg gaggtagaga tcatatggtt
     1441 ctccacgaat acgttaacgc cgcaggcatc actggcggta gtggaggacg cgaccatatg
     1501 gtactacatg aatatgtcaa tgcagccgga ataaccggag ggtccggagg ccgggatcac
     1561 atggtgctgc atgagtatgt gaacgcggcg ggtataactg gtgggtcggg cggacgagac
     1621 catatggtgc ttcacgaata cgtaaacgca gctggcatta ctagttaccc atacgatgtt
     1681 ccagattacg cttaaagcgg aaagctctag atagtgcatg cgaagaggcg aaggtgagtg
     1741 ctccttgggg aagccaatac tcggcaatga aggtgcactg acagaaaaat atatgaccca
     1801 cttctcacat aaccataaat actaacaaga atttctctgt taggttatga aattaatcat
     1861 aaagctgttc aaaagtacgc agtaaaattt tattgcttaa aacgttgcat acttttaggt
     1921 tactttaact tatttacatg gctttatcta gcagactttc gttattctag caattgttta
     1981 cttatattgt ttaaaaaata tgaaaattgt tttccatgca ccttgtcctt gtgtgccgca
     2041 gttattgtta ttgagttaga caatttagcc gacaaccatt ggaaacattt tgaatcgatt
     2101 caacgcacac gcaattgcat tctttttctg ctgttgtccc tagctgacaa gtttcaattt
     2161 tcaatgctcg aagccagacc cgaaattgat attgcctgag ttgagtggaa agtgcagcag
     2221 tttgcaaagg aaccgaaacc attgtttagc tttaaaaaaa aaaaaaagaa ccaaagggga
     2281 gaactttctg aacctagagc caaagtttta tcagaccttt atcggacctg tcatctgcac
     2341 ccagcaaacc atccaagcaa ccgaccagcc atgttggtca atgcattaga attatgccac
     2401 acagctcggt ggaaacattg ccgcttgaaa agcaagctaa accgaaatct aaatcgggaa
     2461 atgggttttt atctacaaaa agtgagcggg aatggcgcgc acaagttttg aatagttacc
     2521 atttgagttt gctgaaggaa gagccatctg gatgtggcag cagttaaatc tttgccattc
     2581 attcgttttc atttgcaaaa ggcaacatga aagttaaatt gagtaacatt aatttgaaga
     2641 atttcctgct tagttgggcg ccccgccatt tcgatgaact tttctgtgaa tgcacaaagt
     2701 ttttgagcac cccaatgccc cccctcaatt cacacttcac taatgaatcc ttgcaattgt
     2761 aacatccttg cagacgcttc tgctaaaccg accagcggcc gcaattatag gctaggtgga
     2821 ggctcagtga tgataagtct gcgatggtgg atgcatgtgt catggtcata gctgtttcct
     2881 gtgtgaaatt gttatccgct cagagggcac aatcctattc cgcgctatcc gacaatctcc
     2941 aagacattag gtggagttca gttcggcgta tggcatatgt cgctggaaag aacatgtgag
     3001 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     3061 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     3121 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     3181 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     3241 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     3301 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     3361 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     3421 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     3481 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     3541 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     3601 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3661 ctacggggtc tgacgctcta ttcaacaaag ccgccgtccc gtcaagtcag cgtaaatggg
     3721 tagggggctt caaatcgtcc tcgtgatacc aattcggagc ctgctttttt gtacaaactt
     3781 gttgataatg gcaattcaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     3841 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     3901 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     3961 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     4021 cgagagccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     4081 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     4141 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     4201 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     4261 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     4321 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggtagcactg
     4381 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     4441 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     4501 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     4561 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     4621 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     4681 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     4741 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     4801 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     4861 aaagtgccag atacctgaaa caaaacccat cgtacggcca aggaagtctc caataactgt
     4921 gatccaccac aagcgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtcat
     4981 gcataatccg cacgcatctg gaaggaagtg ccattccgcc tgacct
//
