LOCUS       sequence-442858-       11543 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for generating 10xUAS-5-HT7R-7xGFP11-HA transgenic flies 
            with attP/attB integration. It carries the attB sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11543)
  AUTHORS   Mallick A, Tan HL, Epstein JM, Gaudry Q, Dacks AM
  TITLE     Serotonin acts through multiple cellular targets during an olfactory
            critical period.
  JOURNAL   bioRxiv [Preprint]. 2024 Apr 14:2024.04.14.589413. doi: 
            10.1101/2024.04.14.589413.
  PUBMED    38645269
REFERENCE   2  (bases 1 to 11543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Apr 14:2024.04.14.589413. doi: 
            10.1101/2024.04.14.589413."
COMMENT     Sequence Label: pACUH 5-HT7R-7xGFP11-HA
FEATURES             Location/Qualifiers
     source          1..11543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    2434..2503
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    join(3047..3077,3078..3279)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3360..3454
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    3478..3572
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3591..3829
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     3776..3797
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     regulatory      3864..3873
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5607..5654
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             5688..5735
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             5751..5798
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             5814..5861
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             5877..5924
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             5940..5987
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             6003..6050
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             6054..6080
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          6189..6254
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6384..6404
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6676..6810
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6713..6732)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6767..6786
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6837..6855)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(7307..7378,7780..8053,8128..8782,8844..9159,9363..9494,
                     9565..10179)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(10958..11512,11513..11543)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tcgacatgcc cgccgtgacc gtcgagaacc cgctgacgct
     2281 gccccgcgta tccgcacccg ccgacgccgt cgcacgtccc gtgctcaccg tgaccaccgc
     2341 gcccagcggt ttcgagggcg agggcttccc ggtgcgccgc gcgttcgccg ggatcaacta
     2401 ccgccacctc gacccgttca tcatgatgga ccagatgggt gaggtggagt acgcgcccgg
     2461 ggagcccaag ggcacgccct ggcacccgca ccgcggcttc gagaccgtga cctacatcgt
     2521 cgatatgcgg tgtgaaatac cgcaccgaat cgcgcggaac taacgacagt cgctccaagg
     2581 tcgtcgaaca aaaggtgaat gtgttgcgga gagcgggtgg gagacagcga aagagcaact
     2641 acgaaacgtg gtgtggtgga ggtgaattat gaagagggcg cgcgatttga aaagtatgta
     2701 tataaaaaat atatcccggt gttttatgta gcgataaacg agtttttgat gtaaggtatg
     2761 caggtgtgta agtcttttgg ttagaagaca aatccaaagt ctacttgtgg ggatgttcga
     2821 aggggaaata cttgtattct ataggtcata tcttgttttt attggcacaa atataattac
     2881 attagctttt tgagggggca ataaacagta aacacgatgg taataatggt aaaaaaaaaa
     2941 aacaagcagt tatttcggat atatgtcggc tactccttgc gtcgggcccg aagtcttaga
     3001 gccagatatg cgagcacccg gaagctcacg atgagaatgg ccagaccatg atgaaataac
     3061 ataaggtggt cccgtcggca agagacatcc acttaacgta tgcttgcaat aagtgcgagt
     3121 gaaaggaata gtattctgag tgtcgtattg agtctgagtg agacagcgat atgattgttg
     3181 attaaccctt agcatgtccg tggggtttga attaactcat aatattaatt agacgaaatt
     3241 atttttaaag ttttattttt aataatttgc gagtacgcaa agcttggctg catccaacgc
     3301 gttgggagct ctccggatcc aagctggccg cggctcgatc cgcttgcatg cctgcaggtc
     3361 ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga
     3421 gtactgtcct ccgagcggag tactgtcctc cgagcggaag cttgcatgcc tgcaggtcgg
     3481 agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt
     3541 actgtcctcc gagcggagta ctgtcctccg agcggagact ctagcgagcg ccggagtata
     3601 aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc
     3661 gctaagcgaa agctaagcaa ataaacaagc gcagctgaac aagctaaaca atctgcagta
     3721 aagtgcaagt taaagtgaat caattaaaag taaccagcaa ccaagtaaat caactgcaac
     3781 tactgaaatc tgccaagaag taattattga atacaagaag agaactctga atagggaatt
     3841 gggaattccg gaatttcctc gaggccacca tggctttatc tggacaggac tggcggcgcc
     3901 atcagagcca ccgccagcac agaaaccaca gaacccaggg aaaccaccag aaactgatct
     3961 ccaccgccac gctgactctg ttcgtgctct ttctgagcag ctggatagcc tatgcggcgg
     4021 gcaaggccac cgttcccgct ccgctcgtgg aaggcgagac ggagtcggcc acttcgcagg
     4081 actttaatag cagtagcgcc ttcctggggg ccatagcctc ggcctcatcg acgggatcgg
     4141 gatcgagctc gggctcggga tcgggatcgg gatcgggatc gggatcctat ggcctggcct
     4201 cgatgaacag cagccccatt gccatagtct cgtatcaagg catcaccagc accaatttgg
     4261 gcgacagcaa tacgactttg gtgccgctat cggacactcc tttgcttttg gaggaatttg
     4321 cagccggcga gtttgtactg cccccgttga cgtccatatt cgtgagcatt gtccttctca
     4381 tcgtgatcct gggcactgtt gtgggcaatg tcctggtctg catagccgtt tgcatggtgc
     4441 ggaaattgag gagaccctgc aattaccttt tggtgtcctt ggctctttcg gatctctgtg
     4501 tggctcttct ggtgatgccc atggccttgc tttatgaagt gctggagaag tggaactttg
     4561 gaccgctgct ctgcgacatc tgggtgtcct tcgatgtgct gtgctgcacg gcctcgattc
     4621 tgaatctgtg tgccatttcc gtggatcgat acctggccat cacgaagccc ctggagtacg
     4681 gcgtgaagag gactcctcgg cgaatgatgc tgtgcgtggg catcgtctgg ctggcagccg
     4741 cctgcatctc attgcctcct ctgctgatcc tgggcaacga gcacgaggac gaggagggcc
     4801 agcccatctg cacggtgtgc cagaacttcg catatcagat ctacgccacc ctgggctcat
     4861 tttacatacc actctcggtg atgctgtttg tgtactatca gatcttcagg gcggcgaggc
     4921 ggattgtcct ggaagagaaa cgcgcccaga cgcatttgca gcaggcgctc aatggcacag
     4981 gatcgccgtc ggccccgcag gctccgccct tgggccacac ggagctggcc agctctggaa
     5041 atggtcagag gcacagcagc gtgggcaaca cttcgctcac ctactccacc tgtgggggtc
     5101 tgagcagcgg aggaggcgca ctggctgggc acggcagtgg gggtggcgtt agtggctcga
     5161 ccggactcct gggctctccg caccacaaga agctgcggtt tcaactggcc aaagagaaga
     5221 aggcctccac aactctgggc atcatcatgt cggcctttac cgtctgctgg ctgccattct
     5281 ttatcctggc cctaattcgt cctttcgaga caatgcacgt accggcatcg ctctcgtctc
     5341 tattcctctg gctgggctat gccaactccc tgctgaaccc tattatctac gccactctga
     5401 acagggattt ccgcaaaccc ttccaggaga tcctctactt ccgctgctcc agcctgaaca
     5461 ccatgatgcg ggagaactac taccaggatc agtatggcga gcctccgtcg cagcgggtga
     5521 tgctgggcga tgagaggcac ggggcgaggg agagctttct cggcgatgga ggaagcggag
     5581 gcgacggagg cagcggagga ggcagccggg accacatggt gctgcacgag tacgtgaacg
     5641 ccgccggcat cactagcgga ggcgacggag gcagcggagg aggcagccgg gaccacatgg
     5701 tgctgcacga gtacgtgaac gccgccggca tcactagcgg tagtggcggc cgtgaccaca
     5761 tggtccttca tgagtatgta aatgctgctg ggattacagg tggctctgga ggtagagatc
     5821 atatggttct ccacgaatac gttaacgccg caggcatcac tggcggtagt ggaggacgcg
     5881 accatatggt actacatgaa tatgtcaatg cagccggaat aaccggaggg tccggaggcc
     5941 gggatcacat ggtgctgcat gagtatgtga acgcggcggg tataactggt gggtcgggcg
     6001 gacgagacca tatggtgctt cacgaatacg taaacgcagc tggcattact agttacccat
     6061 acgatgttcc agattacgct taagcggccg cggctcgagg gtacctctag aggatctttg
     6121 tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa
     6181 gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg
     6241 tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct
     6301 ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg
     6361 ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact
     6421 ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt
     6481 gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa
     6541 aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag
     6601 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     6661 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     6721 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     6781 ctcatcaatg tatcttatca tgtctggatc cactagaagg cctaattcta gtatgtatgt
     6841 aagttaataa aacccatttt tgcggaaagt agataaaaaa aacatttttt ttttttactg
     6901 cactggatat cattgaactt atctgatcag ttttaaattt acttcgatcc aagggtattt
     6961 gatgtaccag gttctttcga ttacctctca ctcaaaatga cattccactc aaagtcagcg
     7021 ctgtttgcct ccttctctgt ccacagaaat atcgccgtct ctttcgccgc tgcgtccgct
     7081 atctctttcg ccaccgtttg tagcgttacg tagcgtcaat gtccgccttc agttgcattt
     7141 tgtcagcggt ttcgtgacga agctccaagc ggtttacgcc atcaattaaa cacaaagtgc
     7201 tgtgccaaaa ctcctctcgc ttcttatttt tgtttgtttt ttgagtgatt ggggtggtga
     7261 ttggttttgg gtgggtaagc aggggaaagt gtgaaaaatc ccggcaatgg gccaagagga
     7321 tcaggagcta ttaattcgcg gaggcagcaa acacccatct gccgagcatc tgaacaatgt
     7381 gagtagtaca tgtgcataca tcttaagttc acttgatcta taggaactgc gattgcaaca
     7441 tcaaattgtc tgcggcgtga gaactgcgac ccacaaaaat cccaaaccgc aattgcacaa
     7501 acaaatagtg acacgaaaca gattattctg gtagctgttc tcgctatata agacaatttt
     7561 tgagatcata tcatgatcaa gacatctaaa ggcattcatt ttcgactata ttctttttta
     7621 caaaaaatat aacaaccaga tattttaagc tgatcctaga tgcacaaaaa ataaataaaa
     7681 gtataaacct acttcgtagg atacttcggg gtactttttg ttcggggtta gatgagcata
     7741 acgcttgtag ttgatatttg agatccccta tcattgcagg gtgacagcgg agcggcttcg
     7801 cagagctgca ttaaccaggg cttcgggcag gccaaaaact acggcacgct ccggccaccc
     7861 agtccgccgg aggactccgg ttcagggagc ggccaactag ccgagaacct cacctatgcc
     7921 tggcacaata tggacatctt tggggcggtc aatcagccgg gctccggatg gcggcagctg
     7981 gtcaaccgga cacgcggact attctgcaac gagcgacaca taccggcgcc caggaaacat
     8041 ttgctcaaga acggtgagtt tctattcgca gtcggctgat ctgtgtgaaa tcttaataaa
     8101 gggtccaatt accaatttga aactcagttt gcggcgtggc ctatccgggc gaacttttgg
     8161 ccgtgatggg cagttccggt gccggaaaga cgaccctgct gaatgccctt gcctttcgat
     8221 cgccgcaggg catccaagta tcgccatccg ggatgcgact gctcaatggc caacctgtgg
     8281 acgccaagga gatgcaggcc aggtgcgcct atgtccagca ggatgacctc tttatcggct
     8341 ccctaacggc cagggaacac ctgattttcc aagccatggt gcggatgcca cgacatctga
     8401 cctatcggca gcgagtggcc cgcgtggatc aggtgatcca ggagctttcg ctcagcaaat
     8461 gtcagcacac gatcatcggt gtgcccggca gggtgaaagg tctgtccggc ggagaaagga
     8521 agcgtctggc attcgcctcc gaggctctaa ccgatccgcc gcttctgatc tgcgatgagc
     8581 ccacctccgg actggactcc tttaccgccc acagcgtcgt ccaggtgctg aagaagctgt
     8641 cgcagaaggg caagaccgtc atcctgacca ttcatcagcc gtcttccgag ctgtttgagc
     8701 tctttgacaa gatccttctg atggccgagg gcagggtagc tttcttgggc actcccagcg
     8761 aagccgtcga cttcttttcc tagtgagttc gatgtgttta ttaagggtat ctagtattac
     8821 ataacatctc aactcctatc cagcgtgggt gcccagtgtc ctaccaacta caatccggcg
     8881 gacttttacg tacaggtgtt ggccgttgtg cccggacggg agatcgagtc ccgtgatcgg
     8941 atcgccaaga tatgcgacaa ttttgccatt agcaaagtag cccgggatat ggagcagttg
     9001 ttggccacca aaaatctgga gaagccactg gagcagccgg agaatgggta cacctacaag
     9061 gccacctggt tcatgcagtt ccgggcggtc ctgtggcgat cctggctgtc ggtgctcaag
     9121 gaaccactcc tcgtaaaagt gcgacttatt cagacaacgg tgagtggttc cagtggaaac
     9181 aaatgatata acgcttacaa ttcttggaaa caaattcgct agattttaga tagaattgcc
     9241 tgattccaca cccttcttag tttttttcaa tgagatgtat agtttatagt tttgcagaag
     9301 ataaataaat ttcatttaac tcgcgaatat taatgagatg cgagtaacat tttaatttgc
     9361 agatggttgc catcttgatt ggcctcatct ttttgggcca acaactcacg caagtgggtg
     9421 tgatgaatat caacggagcc atcttcctct tcctgaccaa catgaccttt caaaacgtct
     9481 ttgccacgat aaatgtaagt catgtttaga atacatttgc atttcaataa tttactaact
     9541 ttctaatgaa tcgattcgat ttaggtgttc acctcagagc tgccagtttt tatgagggag
     9601 gcccgaagtc gactttatcg ctgtgacaca tactttctgg gcaaaacgat tgccgaattg
     9661 ccgctttttc tcacagtgcc actggtcttc acggcgattg cctatccgat gatcggactg
     9721 cgggccggag tgctgcactt cttcaactgc ctggcgctgg tcactctggt ggccaatgtg
     9781 tcaacgtcct tcggatatct aatatcctgc gccagctcct cgacctcgat ggcgctgtct
     9841 gtgggtccgc cggttatcat accattcctg ctctttggcg gcttcttctt gaactcgggc
     9901 tcggtgccag tatacctcaa atggttgtcg tacctctcat ggttccgtta cgccaacgag
     9961 ggtctgctga ttaaccaatg ggcggacgtg gagccgggcg aaattagctg cacatcgtcg
    10021 aacaccacgt gccccagttc gggcaaggtc atcctggaga cgcttaactt ctccgccgcc
    10081 gatctgccgc tggactacgt gggtctggcc attctcatcg tgagcttccg ggtgctcgca
    10141 tatctggctc taagacttcg ggcccgacgc aaggagtagc cgacatatat ccgaaataac
    10201 tgcttgtttt ttttttttac cattattacc atcgtgttta ctgtttattg ccccctcaaa
    10261 aagctaatgt aattatattt gtgccaataa aaacaagata tgacctatag aatacaagta
    10321 tttccccttc gaacatcccc acaagtagac tttggatttg tcttctaacc aaaagactta
    10381 cacacctgca taccttacat caaaaactcg tttatcgcta cataaaacac cgggatatat
    10441 tttttatata catacttttc aaatcgcgcg ccctcttcat aattcacctc caccacacca
    10501 cgtttcgtag ttgctctttc gctgtctccc acccgctctc cgcaacacat tcaccttttg
    10561 ttcgacgacc ttggagcgac tgtcgttagt tccgcgcgat tcggttcgct caaatggttc
    10621 cgagtggttc atttcgtctc aatagaaatt agtaataaat atttgtatgt acaatttatt
    10681 tgctccaata tatttgtata tatttccctc acagctatat ttattctaat ttaatattat
    10741 gactttttaa ggtaattttt tgtgacctgt tcggagtgat tagcgttaca atttgaactg
    10801 aaagtgacat ccagtgtttg ttccttgtgt agatgcatct caaaaaaatg gtgggcataa
    10861 tagtgttgtt tatatatatc aaaaataaca actataataa taagaataca tttaatttag
    10921 aaaatgcttg gatttcactg gaactagaat taattcggct gctgctctaa acgacgcatt
    10981 tcgtactcca aagtacgaat tttttccctc aagctcttat tttcattaaa caatgaacag
    11041 gacctaacgc acagtcacgt tattgtttac ataaatgatt ttttttacta ttcaaactta
    11101 ctctgtttgt gtactcccac tggtatagcc ttcttttatc ttttctggtt caggctctat
    11161 cactttacta ggtacggcat ctgcgttgag tcgcctcctt ttaaatgtct gaccttttgc
    11221 aggtgcagcc ttccactgcg aatcattaaa gtgggtatca caaatttggg agttttcacc
    11281 aaggctgcac ccaaggctct gctcccacaa ttttctctta atagcacact tcggcacgtg
    11341 aattaatttt actccagtca cagctttgca gcaaaatttg caatatttca ttttttttta
    11401 ttccacgtaa gggttaatgt tttcaaaaaa aaattcgtcc gcacacaacc tttcctctca
    11461 acaagcaaac gtgcactgaa tttaagtgta tacttcggta agcttcggct atcgacggga
    11521 ccaccttatg ttatttcatc atg
//
