LOCUS       sequence-442866-        7250 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector for expression of transposase (CMV 
            promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7250)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 7250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pCMV-P1_AG-Tn
FEATURES             Location/Qualifiers
     source          1..7250
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3438..3456)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3439..3456)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3476..3493)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3482..3706
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3752..4180
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3839..3858)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4049..4070
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4189..4209)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4194..4523
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4374..4509
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4436..4455
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    4689..4810
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4726..4745)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4780..4799
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4859..4875)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4859..4875)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4872..4894)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4883..4899
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4907..4913,4914..4931,4932..4937))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4952..4973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5090..5107)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5261..5849)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5341..5360)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6020..6811,6812..6880))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6643..6662
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6881..6985)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7060..7079)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacggc cgccagtgtg ctggaattcg ccgccaccat
      721 gagcagcaac tacaagtgct gcgtggcctt ctgcaagaac aacagataca acgtgaagaa
      781 ggtgggcgtg aacgtgtgct tccacaagtt ccccgagggc aaggagacaa agcagaagtg
      841 gatcgccttc tgccagagag agatcagctg gatccccagc agcagcaatg ttgtgtgcag
      901 ccagcacttt ctgcctagcg actaccaact gagcagcagc cacaacacaa agagaggcgc
      961 caactggctt aatcctgagg ctgtgccctc tattctgctg cctcaggaca caggactgaa
     1021 cctgagccag ttcaacgagc accagcagaa caacaacaac gacgagagca gcagagccga
     1081 actgcaagga gctacaagca gccatctgct gcctgacgag aacgacctga tcctgatcgg
     1141 caaggagaac gtgtgcaaga agtgcaaggg cctgcagcag aagatcttca acctggaggc
     1201 caagctgaat gagctggagg agagaaacaa gcagctggtg accatcaaca acaagctgag
     1261 cgacaccctg aaggaggtga acgagaagga gaaggagcac ctgaagaagc tggaggagct
     1321 ggacaaggcc accaagaaga tcaaggagga gtggcccaga gctaacttcg tgcagaacat
     1381 gaaggacagc ctgaagggaa gcctgagcag caaccagatc gacctgatcc tgggcatcaa
     1441 gaagaccgtg agatggacca aggaggagct gagcctggct ttcaccctga gatacttcag
     1501 ccagagagcc tacagataca tcggcgacga catgaagatt cccgtgcccg tgcctagaac
     1561 cctgcagaga tacagcagca agatcgacct gaagcagggc atcctggagg acatcctgaa
     1621 gttcatcggc agctacagcc aaaccctgaa gcccatggac agagagtgcg tgctgagctt
     1681 cgacgagatg aaggtgagca gagtgctgga atatgacccc agcgccgatg aaattgtggg
     1741 ccccttcaac tatctgcagg tggtgatgat gagaggcctg ttcaaacagt ggaagcagcc
     1801 catcttcatc ggcttcgaca ccaagatgac caaggacatc atcatcgaga tcatcagcag
     1861 actgagcgag aaggacatca acgtggtggc catcatcagc gacaactgcc agaccaacat
     1921 cggctgctgg aaagaactgg gcgccagaga tgatgtggag aagccctatt tcccccaccc
     1981 caagaccaac aagaacgtgt acgtgatccc cgacacccct catcttctga agctgctgag
     2041 aaactggctt ctggaccacg gcttcgagta taacggccag ctgatcgaga caaccaacct
     2101 gctgagaatg gtggccaaga gaatggagag cgagatgacc cccctgttca agcttaccac
     2161 cagccacatt gacatgaccc cccaggagag acagaacgtg agaagagctg ccgagctgct
     2221 gtctagaacc acagccgtgg ctctgagaac atacttcccc gacgacgaca acgctaagat
     2281 cctggccaac ttcatcgaga aggtggacgt gtggttcagc atcagcaaca gctacacccc
     2341 cttcgccaag ctggacttca agaaggccta caccgccagc gatgatcagg ttagagccct
     2401 gaccgacatg tacgacatca tcagcaacat gaccatcccc ggcaagaatg gcctgcagat
     2461 cttccagaga agcatcatga tgcagatcaa gagcctgcag atggtgttcg ccgacatgaa
     2521 gctgaagcac gacgccaagt tcatctgcac ccacaagctg aaccaggacg tgctggagaa
     2581 cttcttcagc cagctgagac aaatcggcgg cgtgtacgat catcctagcc ccctgagctg
     2641 catctacaga atcagactga tgatcctggg caagaccccc accattctgc acaaccagac
     2701 cacaacagtg gaggccgcta attgtgagca ggacgagttc atcaccacca ccgagagcaa
     2761 tgttgccggc gctagaagca atgacgtgtt catcagcgcc agcatgttcg agaaggccga
     2821 gatcacaccc gaactgcctg acgtgaaggc tatggaggag gccaacaact tcgtgagcca
     2881 ggacgtgagc tctcttagca gcgtgagcga cacaagcatc gagctgcctc accaggaagc
     2941 tgatggcctg agctatatcc tgggctacct ggccaagaaa caccacagcc agttcagcca
     3001 cctgaatctg ggcgagcaca ccttcaagac cagaatcgac cacaactact gccagcctcc
     3061 cacctttgtg catcacctga gctgtggcgg cctgattgaa cctagcgacg agttcctgaa
     3121 cctgggcaag aagatggaga agatcttcct gaagatgaac cccgatggcg gacttctgaa
     3181 aggcgagaga atcgtggaca gaatcaccaa caagatcaag agacacctga ccgaactgcc
     3241 cgtggagatc atcagaagct tcgccaagca gagagtgatc gtgagaatga gatacctgaa
     3301 cctgaaggcc acagctgagc agctgaacaa gatgaagcac aagaagagaa agttcgtgac
     3361 cgagaacacc aaggccgcca agaagatgaa gaagatcatc aacgcggccg ctcgagcatg
     3421 catctagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg
     3481 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3541 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3601 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     3661 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     3721 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     3781 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     3841 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     3901 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     3961 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4021 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4081 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4141 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     4201 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4261 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4321 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     4381 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     4441 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     4501 ttggaggcct aggcttttgc aaaaagctcc ccgaaatgac cgaccaagcg acgcccaacc
     4561 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     4621 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     4681 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     4741 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     4801 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     4861 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     4921 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     4981 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     5041 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     5101 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     5161 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     5221 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     5281 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     5341 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     5401 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     5461 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     5521 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     5581 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     5641 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     5701 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     5761 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     5821 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     5881 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     5941 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     6001 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     6061 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     6121 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     6181 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     6241 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     6301 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     6361 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     6421 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     6481 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     6541 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     6601 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     6661 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     6721 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     6781 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     6841 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     6901 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     6961 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     7021 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
     7081 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
     7141 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
     7201 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
//
