LOCUS       sequence-44287-e        5026 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Danielian PS, Friesenhahn LB, Faust AM, West JC, Caron AM, Bronson 
            RT, Lees JA
  TITLE     E2f3a and E2f3b make overlapping but different contributions to 
            total E2f3 activity.
  JOURNAL   Oncogene. 2008 Nov 20;27(51):6561-70. Epub 2008 Jul 28.
  PUBMED    18663357
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene.";
            date: "2008-11-20"; volume: "27(51)"; pages: "6561-70. Epub 2008 Jul
            28"
COMMENT     Sequence Label: pBR.DT-A
FEATURES             Location/Qualifiers
     source          1..5026
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(10..15,16..32,33..38)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     194..213
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        194..212
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    402..626
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     615..632
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     intron          700..765
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(842..1414)
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     promoter        complement(1441..1940)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1489..1508)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     1882..1905
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     complement(1965..1981)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1966..1982)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(2011..2031)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2011..2029)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             2580..2771
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(2765..2787)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    2873..3013
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2928..2947
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3028..3045)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3199..3787)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3279..3298)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3958..4749,4750..4818))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4581..4600
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4819..4923)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4991..5009
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
      181 gcgggatcgc gcgtaatacg actcactata gggcgaattg gagctccacc gcggtggcgg
      241 ccgctctaga ggccgcaatg gccttaatta agtttaaacg gccgacttgg ccggccggcc
      301 tctagaggcc caatggcctt aattaagttt aaacggccga cttggccggc cggcctctag
      361 atgcatgctc gagccccagc tggttctttc cgcctcagaa gccatagagc ccaccgcatc
      421 cccagcatgc ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc
      481 ccccagaata gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga
      541 aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac
      601 agatggctgg caactagaag gcacagtcga ggctgatcag cgagctctag gatctgcatt
      661 ccaccactgc tcccattcat cagttccata ggttggaatc taaaatacac aaacaattag
      721 aatcagtagt ttaacacatt atacacttaa aaattttata tttaccttag agctttaaat
      781 ctctgtaggt agtttgtcca attatgtcac accacagaag taaggttcct tcacaaagag
      841 atcgcctgac acgatttcct gcacaggctt gagccatata ctcatacatc gcatcttggc
      901 cacgttttcc acgggtttca aaattaatct caagttctac gcttaacgct ttcgcctgtt
      961 cccagttatt aatatattca acgctagaac tcccctcagc gaagggaagg ctgagcacta
     1021 cacgcgaagc accatcaccg aaccttttga taaactcttc cgttccgact tgctccatca
     1081 acggttcagt gagacttaaa cctaactctt tcttaatagt ttcggcatta tccactttta
     1141 gtgcgagaac tttcgtcagt cctggatacg tcactttgac cacgcctcca gcttttccag
     1201 agagcgggtt ttcattatct acagagtatc ccgcagcgtc gtatttattg tcggtactat
     1261 aaaacccttt ccaatcatcg tcataatttc cttgtgtacc agattttggc ttttgtatac
     1321 ctttttgaat ggaatctaca taaccaggtt tagtcccgtg gtacgaagaa aagttttcca
     1381 tcacaaaaga tttagaagaa tcaacaacat catcaggatc catggcgagg acctgcaggt
     1441 cgaaaggccc ggagatgagg aagaggagaa cagcgcggca gacgtgcgct tttgaagcgt
     1501 gcagaatgcc gggcctccgg aggaccttcg ggcgcccgcc ccgcccctga gcccgcccct
     1561 gagcccgccc ccggacccac cccttcccag cctctgagcc cagaaagcga aggagcaaag
     1621 ctgctattgg ccgctgcccc aaaggcctac ccgcttccat tgctcagcgg tgctgtccat
     1681 ctgcacgaga ctagtgagac gtgctacttc catttgtcac gtcctgcacg acgcgagctg
     1741 cggggcgggg gggaacttcc tgactagggg aggagtagaa ggtggcgcga aggggccacc
     1801 aaagaacgga gccggttggc gcctaccggt ggatgtggaa tgtgtgcgag gccagaggcc
     1861 acttgtgtag cgccaagtgc ccagcggggc tgctaaagcg catgctccag actgccttgg
     1921 gaaaagcgcc tcccctaccc ggtagaattc gatatcaagc ttatcgatac cgtcgacctc
     1981 gagggggggc ccggtaccca gcttttgttc cctttagtga gggttaattg cgcgcttggc
     2041 agaacatatc catcgcgtcc gccatctcca gcagccgcac gcggcgcatc tcgggcagcg
     2101 ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct
     2161 ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg
     2221 actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg
     2281 tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc cggatctgca
     2341 tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg aagcgctggc
     2401 attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt tgtttaccct
     2461 cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg agcatcctct
     2521 ctcgtttcat cggtatcatt acccccatga acagaaatcc cccttacacg gaggcatcag
     2581 tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc cagacattaa
     2641 cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc tgtgaatcgc
     2701 ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg
     2761 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     2821 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     2881 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     2941 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     3001 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     3061 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     3121 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     3181 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3241 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3301 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3361 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     3421 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3481 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3541 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3601 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     3661 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     3721 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3781 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     3841 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     3901 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     3961 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     4021 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     4081 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     4141 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     4201 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     4261 tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     4321 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     4381 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     4441 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     4501 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     4561 ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     4621 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     4681 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     4741 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     4801 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     4861 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     4921 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     4981 aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaa
//
