LOCUS       sequence-442876-        9050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9050)
  AUTHORS   You J, Croyle JL, Nishimura A, Ozato K, Howley PM
  TITLE     Interaction of the bovine papillomavirus E2 protein with Brd4 
            tethers the viral DNA to host mitotic chromosomes.
  JOURNAL   Cell. 2004 Apr 30. 117(3):349-60.
  PUBMED    15109495
REFERENCE   2  (bases 1 to 9050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2004 
            Apr 30. 117(3):349-60."
COMMENT     Sequence Label: p5068 pGEX-6P-1 Brd4 full-length
FEATURES             Location/Qualifiers
     source          1..9050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     31..48
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        121..198
                     /gene="lacI"
                     /label=lacI promoter
     CDS             199..1281
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(218..237)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(1330..1335,1336..1353,1354..1360)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1368..1384
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1392..1408
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1392..1408
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1404..1931
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGISLSTARCTNASGVR
                     QPSEAVVWLCRS"
     primer_bind     complement(1424..1441)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1424..1440)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1433..1455)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        join(2033..2038,2039..2054,2055..2061)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    2069..2085
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             2108..2761
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     2719..2741
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             2768..2791
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             5012..5029
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6950..6972)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7117..7135)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7203..7307
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7308..7376,7377..8168)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7526..7545)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8339..8927
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8828..8847
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
       61 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg cataaattcc
      121 gacaccatcg aatggcgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt
      181 caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt
      241 gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg
      301 cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa
      361 caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac
      421 gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg
      481 gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt
      541 ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt
      601 gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca
      661 cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg
      721 gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg
      781 cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg
      841 gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat
      901 gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg
      961 cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac
     1021 gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc
     1081 ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag
     1141 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccacgctagc gcccaatacg
     1201 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     1261 cgactggaaa gcgggcagtg agcgcaacgc aattaatgta agttagctca ctcattaggc
     1321 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     1381 acaatttcac acaggaaaca gctatgacca tgattacgga ttcactggcc gtcgttttac
     1441 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     1501 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     1561 gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa
     1621 gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga
     1681 tgcacggtta cgatgcgccc atctacacca acgtgaccta tcccattacg gtcaatccgc
     1741 cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa
     1801 gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttggaatt agcttatcga
     1861 ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg gtatggctgt
     1921 gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt tctggataat
     1981 gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc tgttgacaat
     2041 taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     2101 agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc aacccactcg
     2161 acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc gcgatgaagg
     2221 tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc ttccttatta
     2281 tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata tagctgacaa
     2341 gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc ttgaaggagc
     2401 ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact ttgaaactct
     2461 caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag atcgtttatg
     2521 tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt tgtatgacgc
     2581 tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa aattagtttg
     2641 ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat ccagcaagta
     2701 tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc atcctccaaa
     2761 atcggatctg gaagttctgt tccaggggcc cctgggatcc atgtctgcgg agagcggccc
     2821 tgggacgaga ttgagaaatc tgccagtaat gggggatgga ctagaaactt cccaaatgtc
     2881 tacaacacag gcccaggccc aaccccagcc agccaacgca gccagcacca accccccgcc
     2941 cccagagacc tccaacccta acaagcccaa gaggcagacc aaccaactgc aatacctgct
     3001 cagagtggtg ctcaagacac tatggaaaca ccagtttgca tggcctttcc agcagcctgt
     3061 ggatgccgtc aagctgaacc tccctgatta ctataagatc attaaaacgc ctatggatat
     3121 gggaacaata aagaagcgct tggaaaacaa ctattactgg aatgctcagg aatgtatcca
     3181 ggacttcaac actatgttta caaattgtta catctacaac aagcctggag atgacatagt
     3241 cttaatggca gaagctctgg aaaagctctt cttgcaaaaa ataaatgagc tacccacaga
     3301 agaaaccgag atcatgatag tccaggcaaa aggaagagga cgtgggagga aagaaacagg
     3361 gacagcaaaa cctggcgttt ccacggtacc aaacacaact caagcatcga ctcctccgca
     3421 gacccagacc cctcagccga atcctcctcc tgtgcaggcc acgcctcacc ccttccctgc
     3481 cgtcaccccg gacctcatcg tccagacccc tgtcatgaca gtggtgcctc cccagccact
     3541 gcagacgccc ccgccagtgc ccccccagcc acaaccccca cccgctccag ctccccagcc
     3601 cgtacagagc cacccaccca tcatcgcggc caccccacag cctgtgaaga caaagaaggg
     3661 agtgaagagg aaagcagaca ccaccacccc caccaccatt gaccccattc acgagccacc
     3721 ctcgctgccc ccggagccca agaccaccaa gctgggccag cggcgggaga gcagccggcc
     3781 tgtgaaacct ccaaagaagg acgtgcccga ctctcagcag cacccagcac cagagaagag
     3841 cagcaaggtc tcggagcagc tcaagtgctg cagcggcatc ctcaaggaga tgtttgccaa
     3901 gaagcacgcc gcctacgcct ggcccttcta caagcctgtg gacgtggagg cactgggcct
     3961 acacgactac tgtgacatca tcaagcaccc catggacatg agcacaatca agtctaaact
     4021 ggaggcccgt gagtaccgtg atgctcagga gtttggtgct gacgtccgat tgatgttctc
     4081 caactgctat aagtacaacc ctcctgacca tgaggtggtg gccatggccc gcaagctcca
     4141 ggatgtgttc gaaatgcgct ttgccaagat gccggacgag cctgaggagc cagtggtggc
     4201 cgtgtcctcc ccggcagtgc cccctcccac caaggttgtg gccccgccct catccagcga
     4261 cagcagcagc gatagctcct cggacagtga cagttcgact gatgactctg aggaggagcg
     4321 agcccagcgg ctggctgagc tccaggagca gctcaaagcc gtgcacgagc agcttgcagc
     4381 cctctctcag ccccagcaga acaaaccaaa gaaaaaggag aaagacaaga aggaaaagaa
     4441 aaaagaaaag cacaaaagga aagaggaagt ggaagagaat aaaaaaagca aagccaagga
     4501 acctcctcct aaaaagacga agaaaaataa tagcagcaac agcaatgtga gcaagaagga
     4561 gccagcgccc atgaagagca agccccctcc cacgtatgag tcggaggaag aggacaagtg
     4621 caagcctatg tcctatgagg agaagcggca gctcagcttg gacatcaaca agctccccgg
     4681 cgagaagctg ggccgcgtgg tgcacatcat ccagtcacgg gagccctccc tgaagaattc
     4741 caaccccgac gagattgaaa tcgactttga gaccctgaag ccgtccacac tgcgtgagct
     4801 ggagcgctat gtcacctcct gtttgcggaa gaaaaggaaa cctcaagctg agaaagttga
     4861 tgtgattgcc ggctcctcca agatgaaggg cttctcgtcc tcagagtcgg agagctccag
     4921 tgagtccagc tcctctgaca gcgaagactc cgaaacagag atggctccga agtcaaaaaa
     4981 gaaggggcac cccgggaggg agcagaagaa gcaccatcat caccaccatc agcagatgca
     5041 gcaggccccg gctcctgtgc cccagcagcc gcccccgcct ccccagcagc ccccaccgcc
     5101 tccacctccg cagcagcaac agcagccgcc acccccgcct cccccaccct ccatgccgca
     5161 gcaggcagcc ccggcgatga agtcctcgcc cccacccttc attgccaccc aggtgcccgt
     5221 cctggagccc cagctcccag gcagcgtctt tgaccccatc ggccacttca cccagcccat
     5281 cctgcacctg ccgcagcctg agctgccccc tcacctgccc cagccgcctg agcacagcac
     5341 tccaccccat ctcaaccagc acgcagtggt ctctcctcca gctttgcaca acgcactacc
     5401 ccagcagcca tcacggccca gcaaccgagc cgctgccctg cctcccaagc ccgcccggcc
     5461 cccagccgtg tcaccagcct tgacccaaac acccctgctc ccacagcccc ccatggccca
     5521 acccccccaa gtgctgctgg aggatgaaga gccacctgcc ccacccctca cctccatgca
     5581 gatgcagctg tacctgcagc agctgcagaa ggtgcagccc cctacgccgc tactcccttc
     5641 cgtgaaggtg cagtcccagc ccccaccccc cctgccgccc ccaccccacc cctctgtgca
     5701 gcagcagctg cagcagcagc cgccaccacc cccaccaccc cagccccagc ctccacccca
     5761 gcagcagcat cagccccctc cacggcccgt gcacttgcag cccatgcagt tttccaccca
     5821 catccaacag cccccgccac cccagggcca gcagcccccc catccgcccc caggccagca
     5881 gccacccccg ccgcagcctg ccaagcctca gcaagtcatc cagcaccacc attcaccccg
     5941 gcaccacaag tcggacccct actcaaccgg tcacctccgc gaagccccct ccccgcttat
     6001 gatacattcc ccccagatgt cacagttcca gagcctgacc caccagtctc caccccagca
     6061 aaacgtccag cctaagaaac aggagctgcg tgctgcctcc gtggtccagc cccagcccct
     6121 cgtggtggtg aaggaggaga agatccactc acccatcatc cgcagcgagc ccttcagccc
     6181 ctcgctgcgg ccggagcccc ccaagcaccc ggagagcatc aaggcccccg tccacctgcc
     6241 ccagcggccg gaaatgaagc ctgtggatgt cgggaggcct gtgatccggc ccccagagca
     6301 gaacgcaccg ccaccagggg cccctgacaa ggacaaacag aaacaggagc cgaagactcc
     6361 agttgcgccc aaaaaggacc tgaaaatcaa gaacatgggc tcctgggcca gcctagtgca
     6421 gaagcatccg accaccccct cctccacagc caagtcatcc agcgacagct tcgagcagtt
     6481 ccgccgcgcc gctcgggaga aagaggagcg tgagaaggcc ctgaaggctc aggccgagca
     6541 cgctgagaag gagaaggagc ggctgcggca ggagcgcatg aggagccgag aggacgagga
     6601 tgcgctggag caggcccggc gggcccatga ggaggcacgt cggcgccagg agcagcagca
     6661 gcagcagcgc caggagcaac agcagcagca gcaacagcaa gcagctgcgg tggctgccgc
     6721 cgccacccca caggcccaga gctcccagcc ccagtccatg ctggaccagc agagggagtt
     6781 ggcccggaag cgggagcagg agcgaagacg ccgggaagcc atggcagcta ccattgacat
     6841 gaatttccag agtgatctat tgtcaatatt tgaagaaaat cttttctgag cggccgcatc
     6901 gtgactgact gacgatctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     6961 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     7021 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta
     7081 gcgatagcgg agtgtataat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
     7141 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     7201 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     7261 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     7321 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     7381 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     7441 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     7501 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
     7561 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     7621 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     7681 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     7741 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     7801 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg
     7861 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     7921 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     7981 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     8041 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     8101 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     8161 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     8221 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8281 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     8341 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     8401 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     8461 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     8521 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     8581 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     8641 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     8701 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     8761 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     8821 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     8881 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     8941 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     9001 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
//
